Article
Infectious Diseases
Agata Pruss, Pawel Kwiatkowski, Helena Masiuk, Iwona Bilska, Stefania Giedrys-Kalemba, Barbara Dolegowska
Summary: This study describes the successful containment and elimination of an outbreak of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae (ESBL-KP) in a neonatal ward. Through testing 26 neonates and 42 environmental swabs, it was confirmed that 18 neonates were colonized with ESBL-KP and developed infections. Strengthening hospital hygiene protocols, screening of hospitalized neonates, and cohorting ESBL-KP-positive patients proved effective in controlling and ending the outbreak.
Article
Infectious Diseases
Elena Priante, Chiara Minotti, Cristina Contessa, Margherita Boschetto, Paola Stano, Federico Dal Bello, Ettore De Canale, Elisabetta Lolli, Vincenzo Baldo, Eugenio Baraldi, Daniele Dona
Summary: This study reports the detection and control of an outbreak caused by ESBL-producing Klebsiella pneumoniae (ESBL-KP) in an Italian NICU. Prompt recognition of the event onset and the adoption of infection control interventions helped contain the bacteria spread on the ward. Multidisciplinary management and effective control measures were successful in preventing further cases and controlling the outbreak in a short period of time.
Article
Immunology
Lan Chen, Wenxiu Ai, Ying Zhou, Chunyang Wu, Yinjuan Guo, Xiaocui Wu, Bingjie Wang, Lulin Rao, Yanlei Xu, Jiao Zhang, Liang Chen, Fangyou Yu
Summary: An outbreak of KPC-3-producing Enterobacterales infection occurred in China, with the involvement of clonal expansion and transmission across different species. These KPC-3 strains exhibited high virulence and readily developed resistance. Close monitoring of the spread of bla(KPC-3)-harboring plasmids and these strains is necessary in China and globally.
EMERGING INFECTIOUS DISEASES
(2022)
Article
Multidisciplinary Sciences
F. Gravey, S. Wilson, A. Denisenko, C. Lehoussel, Marguerite Fines-Guyon, S. Le Hello, C. Jeanne-Leroyer
Summary: Whole genome sequencing is now the standard method used for microbiological investigations, enabling the detection of undisclosed outbreaks. By utilizing this approach, we were able to investigate and ultimately halt an uncommon epidemic caused by the extended-spectrum beta-lactamase-producing Klebsiella pneumoniae ST584 strain that spanned over four months in two intensive care units.
Article
Public, Environmental & Occupational Health
Hongmin Xi, Yi Tian, Hui Shao, Xiangyun Yin, Lili Ma, Ping Yang, Xianghong Li
Summary: We describe an outbreak of echovirus 18 infection involving 10 patients in our neonatal intensive care unit, with an attack rate of 33%. The average age at the onset of illness was 26.8 days, and 80% were preterm infants. All patients were discharged home without any lasting effects. There were no significant differences in gestation age, birth weight, delivery mode, use of antibiotics, and parenteral nutrition between the enterovirus (EV) group and non-EV group, but the rate of breastfeeding was significantly higher in the EV group. Separation care and reinforcement of hand-washing seemed to be effective in preventing further spread of the virus. Visiting policy, hygiene practice, and handling of expressed breastmilk should be reinforced.
EPIDEMIOLOGY AND INFECTION
(2023)
Article
Microbiology
Zhien He, Weifeng Xu, Hang Zhao, Wei Li, Yuanyuan Dai, Huaiwei Lu, Liping Zhao, Changfeng Zhang, Yujie Li, Baolin Sun
Summary: Klebsiella pneumoniae, a highly virulent and drug-resistant bacteria, poses a significant threat to global health. This study analyzed 76 isolates of multidrug-resistant hypervirulent K. pneumoniae (MDR-hvKp) and identified multiple plasmids carrying drug resistance genes and virulence genes. The analysis of phenotypic characteristics demonstrated that ST11 MDR-hvKp derived from hypervirulence and multidrug resistance plasmids presents unprecedented challenges in treatment and public health.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Public, Environmental & Occupational Health
I Nakamura, T. Yamaguchi, Y. Miura, H. Watanabe
Summary: This study investigated the transmission of ESBL-producing Klebsiella pneumoniae strains from sinks and drains in a general surgical hospital ward. Through whole-genome analysis, it was found that the isolates were indistinguishable genetically, confirming this mode of transmission.
JOURNAL OF HOSPITAL INFECTION
(2021)
Article
Infectious Diseases
Aminu Seman, Adane Mihret, Shemse Sebre, Tewachew Awoke, Biruk Yeshitela, Berhanu Yitayew, Abraham Aseffa, Daniel Asrat, Tamrat Abebe
Summary: This study reveals a high rate of ESBL and carbapenemase production among Enterobacteriaceae causing bloodstream infections in Ethiopia. Most of the isolates carry multiple types of ESBL genes. These findings are important for understanding and controlling the spread of drug-resistant strains.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Pediatrics
Frida Oldendorff, Agnes Linner, Mikael Finder, Peter Eisenlauer, Malin Kjellberg, Christian G. Giske, Viveka Nordberg
Summary: This case report describes a fatal meningitis caused by ESBL-E. coli ST 1193 in a newborn, highlighting the importance of clinical knowledge about multidrug-resistant clones.
FRONTIERS IN PEDIATRICS
(2022)
Article
Immunology
Xiaoyan Wu, Xiangchen Li, Junjie Yu, Mengli Shen, Chenliang Fan, Yewei Lu, Junshun Gao, Xiaosi Li, Hongsheng Li
Summary: This study investigates the first outbreak of OXA-232-producing carbapenem-resistant Klebsiella pneumoniae (CRKP) in the Second Affiliated Hospital of Jiaxing University. Whole-genome sequencing revealed that all 21 OXA-232-producing CRKP strains belonged to sequence type 15 (ST15) and shared similar resistance, virulence genes, and plasmid types, suggesting clonal transmission between the environment and patients. Integrated genomic and epidemiological analysis traced the outbreak to two clonal transmission clusters, including 14 and 2 patients.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Tom J. B. de Man, Anna Q. Yaffee, Wenming Zhu, Dhwani Batra, Efe Alyanak, Lori A. Rowe, Gillian McAllister, Heather Moulton-Meissner, Sandra Boyd, Andrea Flinchum, Rachel B. Slayton, Steven Hancock, Maroya Spalding Walters, Alison Laufer Halpin, James Kamile Rasheed, Judith Noble-Wang, Alexander J. Kallen, Brandi M. Limbago
Summary: This investigation identified the largest known outbreak of VIM-producing CRE in the United States, showcasing evidence of in-hospital plasmid transmission and environmental contamination.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Immunology
Rindidzani E. Magobo, Husna Ismail, Michelle Lowe, Wilhelmina Strasheim, Ruth Mogokotleng, Olga Perovic, Stanford Kwenda, Arshad Ismail, Manala Makua, Abram Bore, Rose Phayane, Harishia Naidoo, Tanya Dennis, Makhosazane Ngobese, Wim Wijnant, Nelesh P. Govender, Baby Baby GERMS-SA
Summary: Following an increase in carbapenem-resistant Klebsiella pneumoniae (CRKP) bloodstream infections and associated deaths in a neonatal unit of a South Africa hospital, an outbreak investigation was conducted from October 2019 to February 2020, with cross-sectional follow-up from March 2020 to May 2021. Genomic and epidemiologic data were used to analyze transmission networks of outbreak-related clones. A total of 31 CRKP infection cases and 14 deaths were documented, with two major clones (blaNDM-1 ST152 and blaOXA-181 ST307) co-circulating. The blaNDM-1 ST152 clone accounted for the majority of deaths.
EMERGING INFECTIOUS DISEASES
(2023)
Article
Multidisciplinary Sciences
Jacky Dwiyanto, Jia Wei Hor, Daniel Reidpath, Tin Tin Su, Shaun Wen Huey Lee, Qasim Ayub, Faizah Binti Mustapha, Sui Mae Lee, Su Chern Foo, Chun Wie Chong, Sadequr Rahman
Summary: This study compared the resistome and genomic profiles of extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-EC) in the community and clinical settings through whole-genome sequencing. The results showed that ESBL-EC isolates from both settings shared similar resistome profiles, suggesting the exchange of genetic materials through horizontal gene transfer.
Article
Microbiology
Alia Batool, Asma Yaqoob, Zahid Anwar, Lovleen Tina Joshi, Ramsha Batool, Durreshahwar Lone, Zikria Saleem, Qasim Ahmed, Yousef A. Bin Jardn, Mohammed Bourhia, Muhammad Usman Qamar
Summary: This study investigated the outbreak of Burkholderia cepacia complex (BCC) in the neonatal intensive care unit (NICU), including mortality, antimicrobial resistance, and associated risk factors. The findings revealed that the outbreak of NDM-producing BCC resulted in increased neonatal mortality.
FUTURE MICROBIOLOGY
(2023)
Article
Infectious Diseases
Mengsi Zhao, Jing He, Ran Zhang, Junhua Feng, Yanli Deng, Jinyan Zhang
Summary: The epidemiological characteristics of New Delhi Metallo-beta-Lactamase-Producing (NDM) Enterobacteriaceae were analyzed to clarify the distribution characteristics of carbapenem-resistant Enterobacteriaceae (CRE) in hospitals and identify susceptible patients. 42 strains of NDM-producing Enterobacteriaceae, primarily Escherichia coli, Klebsiella pneumoniae, and Enterobacter cloacae, were collected from a hospital in Hebei Medical University. The antimicrobial susceptibility testing results showed high resistance to multiple antibiotics but high sensitivity to amikacin in these strains.
BMC INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
S. Gallah, Y. Benzerara, J. Tankovic, P. -L. Woerther, H. Bensekri, J. -L. Mainardi, G. Arlet, S. Vimont, M. Garnier
CLINICAL MICROBIOLOGY AND INFECTION
(2018)
Article
Infectious Diseases
Paul-Louis Woerther, Taha Jardak, Ines Ben Hassine, Sebastien Forget, Elisabeth Chachaty, Guillaume Arlet, Dominique Decre
MICROBIAL DRUG RESISTANCE
(2018)
Article
Infectious Diseases
Zaineb Hamzaoui, Alain Ocampo-Sosa, Elaa Maamar, Marta Fernandez Martinez, Sana Ferjani, Samia Hammami, Sarra Harbaoui, Nathalie Genel, Guillaume Arlet, Mabrouka Saidani, Amine Slim, Ilhem Boutiba-Ben Boubaker, Luis Martinez-Martinez
MICROBIAL DRUG RESISTANCE
(2018)
Article
Infectious Diseases
Naim Ouldali, Corinne Levy, Emmanuelle Varon, Stephane Bonacorsi, Stephane Bechet, Robert Cohen, Francois Angoulvant
LANCET INFECTIOUS DISEASES
(2018)
Article
Microbiology
Laure Surgers, Anders Boyd, Pierre-Marie Girard, Guillaume Arlet, Dominique Decre
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2019)
Article
Infectious Diseases
Laure Surgers, Peter Boersma, Pierre-Marie Girard, Audrey Homor, Delphine Geneste, Guillaume Arlet, Dominique Decre, Anders Boyd
INFECTION AND DRUG RESISTANCE
(2019)
Article
Infectious Diseases
Maria A. Jure, Marta E. Castillo, Humberto E. Musa, Carolina G. Lopez, Mariel A. Caceres, Silvana D. Mochi, Aurore A. Bousquet, Nathalie A. Genel, Guillaume A. Arlet, Dominique C. Decre
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2019)
Article
Genetics & Heredity
Catherine Branger, Alice Ledda, Typhaine Billard-Pomares, Benoit Doublet, Valerie Barbe, David Roche, Claudine Medigue, Guillaume Arlet, Erick Denamur
MICROBIAL GENOMICS
(2019)
Letter
Infectious Diseases
Cecile Emeraud, Khanh Villageois-Tran, Nathalie Genel, Laure Surgers, Wladimir Sougakoff, Guillaume Arlet, Remy A. Bonnin, Dominique Decre, Catherine Eckert
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2020)
Article
Microbiology
Salah Gallah, Khanh Villageois-Tran, Alexandre Godmer, Guillaume Arlet, Martin Rottman, Yahia Benzerara, Marc Garnier
Summary: The study validated a rapid detection method for carbapenemase-producing Gram-negative bacilli (C-PGNB) using rectal swabs, demonstrating high sensitivity and specificity in accurately detecting C-PGNB colonization in clinical settings.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Infectious Diseases
Rishma Amarsy, David Trystram, Emmanuelle Cambau, Catherine Monteil, Sandra Fournier, Juliette Oliary, Helga Junot, Pierre Sabatier, Raphael Porcher, Jerome Robert, Vincent Jarlier
Summary: This study measured the impact of the first wave of COVID-19 on the incidence of bloodstream infections at the largest multisite public healthcare institution in France. The results showed that the COVID-19 pandemic had a strong impact on hospital management and had unfavorable effects on severe infections, antimicrobial resistance, and laboratory work diagnostics.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Review
Microbiology
Alain Philippon, Guillaume Arlet, Roger Labia, Bogdan I. Iorga
Summary: Class C β-lactamases exhibit molecular variability and can be categorized into two functional groups. These enzymes are encoded by chromosomal and inducible genes and are widely distributed among bacteria. Molecular identification is based on three catalytic motifs, and numerous conserved amino acid residues have been identified. The concept of natural ESACs is supported by the discovery of genetic modifications in pathogenic species and the emergence of resistance to carbapenems and inhibitors.
CLINICAL MICROBIOLOGY REVIEWS
(2022)
Article
Microbiology
Gaelle Gruel, David Couvin, Stephanie Guyomard-Rabenirina, Guillaume Arlet, Jean-Christophe Bambou, Matthieu Pot, Xavier Roy, Antoine Talarmin, Benoit Tressieres, Severine Ferdinand, Sebastien Breurec
Summary: This study determines the prevalence of extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-E) in feces of household and shelter pets in Guadeloupe. The study finds that the only risk factor associated with a higher prevalence of ESBL-E rectal carriage is a stay in a shelter. The study also identifies ESBL-E as mainly Escherichia coli, with certain plasmids carrying antimicrobial resistance genes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Public, Environmental & Occupational Health
Rishma Amarsy, Sandra Fournier, David Trystram, Catherine Monteil, Xavier Raynaud, Vincent Jarlier, Jerome Robert
Summary: The impact of COVID-19 pandemic on bloodstream infections due to Streptococcus pneumoniae and Streptococcus pyogenes was assessed in 25 university hospitals in Paris. The incidence rates of BSIs, which had been stable in 2018 and 2019, decreased for the two pathogens during the two lockdown periods in 2020. Containment policies such as social distancing, masking, and hand hygiene strengthening in community and hospital settings are likely to reduce BSIs caused by these pathogens.
AMERICAN JOURNAL OF INFECTION CONTROL
(2023)
Article
Genetics & Heredity
Catherine Branger, Alice Ledda, Typhaine Billard-Pomares, Benoit Doublet, Stephanie Fouteau, Valerie Barbe, David Roche, Stephane Cruveiller, Claudine Medigue, Miguel Castellanos, Dominique Decre, Laurence Drieux-Rouze, Olivier Clermont, Jeremy Glodt, Olivier Tenaillon, Axel Cloeckaert, Guillaume Arlet, Erick Denamur
MICROBIAL GENOMICS
(2018)