Article
Biotechnology & Applied Microbiology
Xiao-xi Jia, Yuan-yuan Wang, Wen-zhu Zhang, Wen-ge Li, Lu-lu Bai, Jin-xing Lu, Chao-feng Ma, Yuan Wu
Summary: This study developed a new method for detecting toxigenic C. difficile directly from fecal samples using a single-tube multiplex real-time PCR, which showed improved detection efficiency. The method could be performed on various polymerase chain reaction devices, including point-of-care testing.
Article
Multidisciplinary Sciences
Carolina Albuquerque, Davide Pagnossin, Kirsten Landsgaard, Jessica Simpson, Derek Brown, June Irvine, Denise Candlish, Alison E. Ridyard, Gillian Douce, Caroline Millins
Summary: The study indicates that canine carriage of Clostridioides difficile is 18.7%, with 34% of strains being toxigenic. Previous antibiotic treatment is significantly associated with carriage, but toxigenic strains do not correlate with diarrhoeal disease in dogs.
Review
Multidisciplinary Sciences
Tebelay Dilnessa, Alem Getaneh, Workagegnehu Hailu, Feleke Moges, Baye Gelaw
Summary: A high prevalence of C. difficile was observed among hospitalized patients, emphasizing the need for attention and prevention. The resistance of C. difficile to metronidazole and vancomycin was lower compared to other antibiotics.
Article
Public, Environmental & Occupational Health
Florence Skyum, Ming Chen, Christian Backer Mogensen
Summary: This study evaluated a new fast in-house PCR assay for detecting norovirus and C difficile infections, showing acceptable sensitivity and specificity in fecal samples and rectal swabs. The assay reduced time consumption by half, indicating potential for clinical use pending further research to improve accuracy.
AMERICAN JOURNAL OF INFECTION CONTROL
(2022)
Article
Chemistry, Analytical
Waleed A. Hassanain, Julia Spoors, Christopher L. Johnson, Karen Faulds, Neil Keegan, Duncan Graham
Summary: The study introduces a novel testing platform for the rapid and simultaneous detection of two specific biomarkers of Clostridium difficile infection, providing a rapid, selective, sensitive, and cost-effective clinical evaluation method for CDI.
Article
Medicine, General & Internal
Hanane Zerrouki, Sid-Ahmed Rebiahi, Linda Hadjadj, Jean-Marc Rolain, Seydina M. Diene
Summary: This study developed a real-time PCR assay for the simultaneous detection of vanA and vanB genes, showing 100% specificity and positivity on tested vancomycin-resistant isolates. The detection limit of vanA and vanB genes by this assay was 47 CFU/mL and 32 CFU/mL, respectively.
Article
Agriculture, Dairy & Animal Science
Laila Darwich, Chiara Seminati, Jorge R. Lopez-Olvera, Anna Vidal, Laia Aguirre, Marina Cerda, Biel Garcias, Marta Valldeperes, Raquel Castillo-Contreras, Lourdes Migura-Garcia, Carles Conejero, Gregorio Mentaberre
Summary: This study in the Metropolitan Area of Barcelona, Spain found Escherichia coli carrying antimicrobial resistance genes and toxigenic Clostridioides difficile in wild boars, highlighting their role as sentinels of environmental antimicrobial-resistant bacteria sources, especially in urban areas. Wild boars foraging in urban and peri-urban locations were more exposed to antimicrobial-resistant bacteria sources compared to those in natural environments. Further efforts are needed to identify and control potential anthropogenic sources of antimicrobial resistance.
Article
Microbiology
Dorota Wultanska, Pawel Karpinski, Michal Piotrowski, Hanna Pituch
Summary: Sub-MIC concentrations of metronidazole affect the biofilm formation of clinical and reference strains of Clostridioides difficile, particularly inducing biofilm formation in hypervirulent RT027 strains.
Article
Microbiology
Adriana Calderaro, Mirko Buttrini, Monica Martinelli, Benedetta Farina, Tiziano Moro, Sara Montecchini, Maria Cristina Arcangeletti, Carlo Chezzi, Flora De Conto
Summary: This study evaluated a Matrix-Assisted Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS (T-MALDI)) method for rapid classification of circulating C. difficile strains. The results showed that this method has good recognition and validation capabilities, especially for PR1-PR5 ribotyping.
Article
Microbiology
Raymond Kiu, Alexander G. Shaw, Kathleen Sim, Antia Acuna-Gonzalez, Christopher A. Price, Harley Bedwell, Sally A. Dreger, Wesley J. Fowler, Emma Cornwell, Derek Pickard, Gusztav Belteki, Jennifer Malsom, Sarah Phillips, Gregory R. Young, Zoe Schofield, Cristina Alcon-Giner, Janet E. Berrington, Christopher J. Stewart, Gordon Dougan, Paul Clarke, Gillian Douce, Stephen D. Robinson, J. Simon Kroll, Lindsay J. Hall
Summary: Genomic and phenotypic analyses of 272 infant-associated Clostridium perfringens isolates suggest that pfoA is an important toxin gene in C. perfringens linked with preterm infant intestinal diseases. The gene encoding toxin perfringolysin O, pfoA, was largely deficient in a human-derived hypovirulent lineage, as well as certain colonization factors, in contrast to typical pfoA-encoding virulent lineages. These findings suggest both the importance of pfoA(+)C. perfringens as a gut pathogen in preterm infants and areas for further investigation, including potential intervention and therapeutic strategies.
NATURE MICROBIOLOGY
(2023)
Article
Genetics & Heredity
Bilal Ahmed Abbasi, Aishwarya Dharan, Astha Mishra, Devansh Saraf, Irsad Ahamad, Prashanth Suravajhala, Jayaraman Valadi
Summary: In this study, the genomes of six strains of Clostridium difficile were annotated and characterized using in silico approaches. The functional properties of proteins involved in the pathophysiology of the disease were identified, providing valuable information for understanding the biological nature of the strains and developing pharmaceutical targets.
FRONTIERS IN GENETICS
(2022)
Review
Medicine, General & Internal
Ibrahim A. Al-Zahrani
Summary: Clostridioides difficile (C. difficile) infection is a global threat to many healthcare settings. Excessive use of antimicrobials, lack of optimal antibiotic policies, and suboptimal infection control practices have fueled the development of this health issue. Prudent use of antimicrobials and simple infection control measures can significantly reduce infection rates.
SAUDI MEDICAL JOURNAL
(2023)
Article
Chemistry, Analytical
M. G. Rizzo, S. Carnazza, L. M. De Plano, D. Franco, M. S. Nicol, V Zammuto, S. Petralia, Giovanna Calabrese, C. Gugliandolo, S. Conoci, S. P. P. Guglielmino
Summary: The study developed a new method for rapid and sensitive detection of bacteria in blood using engineered M13 phages and PCR in a miniaturized silicon microchip, providing a fast and efficient diagnosis approach.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Multidisciplinary Sciences
Subeen Hong, Seung Mi Lee, Sohee Oh, SoYeon Kim, Young Mi Jung, Sun Min Kim, Chan-Wook Park, Jong Kwan Jun, Byoung Jae Kim, Joong Shin Park
Summary: This study evaluated the performance of a PNA probe-based real-time PCR assay for detecting common aneuploidies. The results showed that the assay had a sensitivity and specificity of 100%. Compared to conventional karyotyping, the PNA probe-based real-time PCR assay was rapid and easy to use.
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Jun Kawase, Tomotake Sakai, Masaaki Iwaki, Kaoru Umeda, Aiko Fukuma, Naoki Fujisawa, Yuta Kawakami, Hiroki Hayashi, Mieko Wada
Summary: A multiplex real-time PCR assay has been developed to rapidly discriminate Corynebacterium ulcerans from Corynebacterium pseudotuberculosis and detect the bacterial diphtheria toxin gene. This assay is a valuable tool for identification of potentially toxigenic C. ulcerans.
JOURNAL OF MICROBIOLOGICAL METHODS
(2022)
Review
Microbiology
Paul Legendre, Valerie Lalande, Catherine Eckert, Frederic Barbut, Laurence Fardet, Jean-Luc Meynard, Laure Surgers
Article
Infectious Diseases
F. Barbut, C. Gouot, N. Lapidus, L. Suzon, R. Syed-Zaidi, V. Lalande, C. Eckert
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2017)
Article
Microbiology
V. Pantaleon, M. Monot, C. Eckert, S. Hoys, A. Collignon, C. Janoir, T. Candela
Article
Microbiology
Catherine Eckert, Thomas Devalliere, Rabab Syed-Zaidi, Valerie Lalande, Frederic Barbut
Article
Infectious Diseases
S. Tschudin-Sutter, E. J. Kuijper, A. Durovic, M. J. G. T. Vehreschild, F. Barbut, C. Eckert, F. Fitzpatrick, M. Hell, T. Noren, J. O'Driscoll, J. Coia, P. Gastmeier, L. von Mueller, M. H. Wilcox, A. F. Widmer
CLINICAL MICROBIOLOGY AND INFECTION
(2018)
Article
Infectious Diseases
Nagham Khanafer, Philippe Vanhems, Frederic Barbut, Catherine Eckert, Michel Perraud, Francois Vandenesch, Christine Luxemburger, Clarisse Demont
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2018)
Article
Infectious Diseases
F. Barbut, N. Day, S. Bouee, A. Youssouf, L. Grandvoinnet, V. Lalande, J. Couturier, C. Eckert
CLINICAL MICROBIOLOGY AND INFECTION
(2019)
Article
Multidisciplinary Sciences
Laurent Ferraris, Jeanne Couturier, Catherine Eckert, Johanne Delannoy, Frederic Barbut, Marie-Jose Butel, Julio Aires
Article
Microbiology
Daniel R. Knight, Brian Kullin, Grace O. Androga, Frederic Barbut, Catherine Eckert, Stuart Johnson, Patrizia Spigaglia, Kazuhiro Tateda, Pei-Jane Tsai, Thomas V. Riley
Letter
Infectious Diseases
Cecile Emeraud, Khanh Villageois-Tran, Nathalie Genel, Laure Surgers, Wladimir Sougakoff, Guillaume Arlet, Remy A. Bonnin, Dominique Decre, Catherine Eckert
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2020)
Article
Microbiology
Kerrie Davies, Georgina Davis, Frederic Barbut, Catherine Eckert, Nicola Petrosillo, Raffaella Pisapia, Barbara Gaertner, Fabian K. Berger, Elena Reigadas, Emilio Bouza, Clarisse Demont, Mark H. Wilcox
Article
Microbiology
Alexandre Godmer, Yahia Benzerara, Anne Cecile Normand, Nicolas Veziris, Salah Gallah, Catherine Eckert, Philipe Morand, Renaud Piarroux, Alexandra Aubry
Summary: MALDI-TOF MS is commonly used in clinical microbiology labs for pathogen identification, but faces limitations, especially in uncertain identification of ECC species like E. hormaechei. By utilizing an online database combined with high-performance MSI software, substantial improvements in species-level identification of ECC isolates can be achieved.
MICROBIOLOGY SPECTRUM
(2021)
Article
Infectious Diseases
J-C Marvaud, S. Quevedo-Torres, C. Eckert, C. Janoir, F. Barbut
NEW MICROBES AND NEW INFECTIONS
(2019)
Article
Microbiology
Jeanne Couturier, Catherine Eckert, Frederic Barbut
Review
Infectious Diseases
Mouna Doufair, Catherine Eckert, Laurence Drieux, Come Amani-Moibeni, Liliane Bodin, Michel Denis, Jean Didier Grange, Guillaume Arlet, Frederic Barbut
