☆ 4.7 Letter

Prevalence of the Operon Encoding Subtilase Cytotoxin in Non-O157 Shiga Toxin-Producing Escherichia coli Isolated from Humans in the United States

JOURNAL OF CLINICAL MICROBIOLOGY (2009)

期刊

JOURNAL OF CLINICAL MICROBIOLOGY

卷 47, 期 9, 页码 3058-3059

出版社

AMER SOC MICROBIOLOGY

DOI: 10.1128/JCM.00706-09

关键词

-

类别

Microbiology

向作者/读者索取更多资源

Protocol

Reagent

作者

我是这篇论文的作者

点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7

评分不足

次要评分

新颖性

-

重要性

-

科学严谨性

-

评价这篇论文

推荐

Article Toxicology

The enzyme subunit SubA of Shiga toxin-producing E. coli strains demonstrates comparable intracellular transport and cytotoxic activity as the holotoxin SubAB in HeLa and HCT116 cells in vitro

Katharina Sessler, Panagiotis Papatheodorou, Fanny Wondany, Maike Krause, Sabrina Noettger, Denise Bernhard, Jens Michaelis, Herbert Schmidt, Holger Barth

Summary: The study found that in the absence of SubB, SubA is internalized into the endoplasmic reticulum (ER) and cleaves the chaperone GRP78, leading to cytotoxic effects. By analyzing a SubA mutant, the C-terminal SEEL motif was identified as an ER-targeting signal.

ARCHIVES OF TOXICOLOGY (2021)

添加到收藏夹

Article Microbiology

Shiga Toxin-Producing Escherichia coli Outbreaks in the United States, 2010-2017

Danielle M. Tack, Hannah M. Kisselburgh, LaTonia C. Richardson, Aimee Geissler, Patricia M. Griffin, Daniel C. Payne, Brigette L. Gleason

Summary: The study summarizes the epidemiology and transmission routes of STEC outbreaks in the United States from 2010 to 2017, finding that foodborne transmission is the most common route, and there are differences between O157 and non-O157 outbreaks in terms of food sources, demographic characteristics, and severity.

MICROORGANISMS (2021)

添加到收藏夹

Article Food Science & Technology

Combined Action of Shiga Toxin Type 2 and Subtilase Cytotoxin in the Pathogenesis of Hemolytic Uremic Syndrome

Romina S. Alvarez, Fernando D. Gomez, Elsa Zotta, Adrienne W. Paton, James C. Paton, Cristina Ibarra, Flavia Sacerdoti, Maria M. Amaral

Summary: In vitro, Stx2 and SubAB had similar effects on renal cell damage individually and together; however, in vivo, co-injection of these toxins reduced survival time, caused weight loss, worsened renal function, and induced erythrocyte morphological alterations in mice more severely compared to separate injections. This study provides valuable information on the in vivo effects of Stx2 and SubAB acting together during STEC infections.

TOXINS (2021)

添加到收藏夹

Article Microbiology

Epidemiology of Non-O157 Shiga Toxin-Producing Escherichia coli in the Province of Alberta, Canada, from 2018 to 2021

Heather Glassman, Christina Ferrato, Linda Chui

Summary: Non-O157 serogroups of Shiga toxin-producing Escherichia coli (STEC) play a significant role in disease burden, but are often overlooked by traditional detection methods. This study investigated the epidemiology of non-O157 STEC in Alberta, Canada from 2018 to 2021. The results showed increased detection during the summer months, with the most common serogroups being O26, O103, O111, O121, O118, and O71. Further characterization of their virulence factors and clinical impact is necessary.

MICROORGANISMS (2022)

添加到收藏夹

Article Multidisciplinary Sciences

Whole genome sequence-based characterisation of Shiga toxin-producing Escherichia coli isolated from game meat originating from several European countries

Magdalena Nueesch-Inderbinen, Andrea Treier, Marc J. A. Stevens, Roger Stephan

Summary: The study assessed the presence of pathogenic bacteria, specifically Shiga toxin-producing Escherichia coli (STEC), in game meat samples from different European countries. PCR analysis detected Shiga-toxin encoding genes in 84% of the samples, with 25% of the same samples containing isolated STEC strains. Various serotypes and sequence types were identified, with some strains carrying virulence factors associated with both STEC and extra-intestinal pathogenic E. coli (ExPEC). The findings suggest that game meat may serve as a potential source of STEC infections in humans, highlighting the importance of safe handling and preparation of such meat to prevent foodborne illnesses.

SCIENTIFIC REPORTS (2023)

添加到收藏夹

Article Immunology

A chimeric protein-based vaccine elicits a strong IgG antibody response and confers partial protection against Shiga toxin-producing Escherichia coli in mice

David A. Montero, Richard Garcia-Betancourt, Roberto M. Vidal, Juliana Velasco, Pablo A. Palacios, Daniela Schneider, Carolina Vega, Leonardo Gomez, Hernan Montecinos, Rodrigo Soto-Shara, Angel Onate, Leandro J. Carreno

Summary: Researchers have developed a chimeric protein-based vaccine targeting seven virulence factors of Shiga toxin-producing Escherichia coli (STEC), which elicited significant immune responses in mice and provided protection against infection. However, further improvement is needed to enhance the vaccine's efficacy and explore other potential mechanisms of protection.

FRONTIERS IN IMMUNOLOGY (2023)

添加到收藏夹

Article Biotechnology & Applied Microbiology

Delayed lactose utilization among Shiga toxin-producing Escherichia coli of serogroup O121

Alexander Gill, Tanis McMahon, Forest Dussault, Karen Jinneman, Rebecca Lindsey, Haley Martin, Devon Stoneburg, Nancy Strockbine, June Wetherington, Peter Feng

Summary: Two outbreaks of Shiga toxin-producing Escherichia coli O121:H19 associated with wheat flour in the USA and Canada involved strains with delayed lactose utilization (DLU), attributed to the insertion sequence IS600. This insertion might reduce the expression of fi-galactoside permease, resulting in the DLU phenotype. Considering the high probability of DLU is important when using lactose-containing media for the isolation of STEC O121.

FOOD MICROBIOLOGY (2022)

添加到收藏夹

Article Agriculture, Dairy & Animal Science

Prevalence of Shiga Toxin-Producing Escherichia coli O157 in Wild Scottish Deer with High Human Pathogenic Potential

Stephen F. Fitzgerald, Mairi C. Mitchell, Anne Holmes, Lesley Allison, Margo Chase-Topping, Nadejda Lupolova, Beth Wells, David L. Gally, Tom N. Mcneilly

Summary: The prevalence of Shiga toxin-producing E. coli (STEC) serogroup O157 in Scottish wild deer was determined to be low (0.28%). However, identified isolates had high virulence potential and were found in deer feces at high levels, indicating a potential risk to humans. The study also identified a wild deer isolate as a possible source of a human outbreak in Scotland. These findings highlight the importance of food hygiene practices during the processing of wild deer carcasses for human consumption.

ANIMALS (2023)

添加到收藏夹

Article Genetics & Heredity

Phylogenetic structure of Shiga toxin- producing Escherichia coli O157:H7 from sub- lineage to SNPs

Timothy J. Dallman, David R. Greig, Saheer E. Gharbia, Claire Jenkins

Summary: Analysis of STEC O157:H7 genomes revealed that 5-SNP single linkage clusters are useful for detecting outbreak-related cases, while 5-SNP single linkage community clusters without identified epidemiological links are more likely to be temporally and/or geographically related. 10-SNP single linkage clusters occur infrequently and are challenging to investigate.

MICROBIAL GENOMICS (2021)

添加到收藏夹

Article Infectious Diseases

Antimicrobial Resistance of Non-O157 Shiga Toxin-Producing Escherichia coli Isolated from Humans and Domestic Animals

Yanyu Pan, Bin Hu, Xiangning Bai, Xi Yang, Lijiao Cao, Qian Liu, Hui Sun, Juan Li, Ji Zhang, Dong Jin, Yanwen Xiong

Summary: This study investigated the antimicrobial resistance of non-O157 Shiga toxin-producing Escherichia coli (STEC) strains in China, revealing diverse resistance patterns and the presence of various resistance genes among strains from different sources. Whole genome analysis showed genetic diversity among STEC strains from different sources, with some strains tending to cluster closely within the phylogenetic tree. These findings provide valuable information for monitoring and guiding the use of antimicrobial drugs in the management of multidrug resistant STEC strains among animals and humans.

ANTIBIOTICS-BASEL (2021)

添加到收藏夹

Article Microbiology

Shiga Toxin (Stx) Phage-Encoded Lytic Genes Are Not Required for the Mouse Virulence of O157:H7 Escherichia coli Stx2-Producing Clinical Isolates

R. R. Atitkar, J. R. Hauser, A. R. Melton-Celsa

Summary: In a murine model of STEC infection, the stx(2a) phage lytic genes were found to be unnecessary for the virulence of pathogenic O157:H7 clinical isolates or for the release of Stx2a into bacterial cultures. These findings suggest an alternative mechanism for Stx2a release from STEC strains.

MICROBIOLOGY SPECTRUM (2023)

添加到收藏夹

Article Immunology

Exploring the nature of interaction between shiga toxin producing Escherichia coli (STEC) and free-living amoeba-Acanthamoeba sp

Margherita Montalbano Di Filippo, Arianna Boni, Paola Chiani, Manuela Marra, Maria Carollo, Lucrezia Cristofari, Fabio Minelli, Arnold Knijn, Stefano Morabito

Summary: This study examines the interaction between STEC strains and Acanthamoeba sp., finding that STEC can survive within these amoebae and maintain their pathogenic potential. The internalization in free-living amoebae allows STEC to resist non-permissive growth conditions, escape detection, and withstand disinfection treatments.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2022)

添加到收藏夹

Article Biotechnology & Applied Microbiology

Shiga Toxin-Producing Escherichia coli (STEC) and STEC-Associated Virulence Genes in Raw Ground Pork in Canada

Helen Zhang, Etsuko Yamamoto, Johanna Murphy, Catherine Carrillo, Annie Locas

Summary: This study investigated the occurrence of STEC in Canadian retail raw ground pork and found the presence of both O157 and non-O157 STEC strains. The virulence gene profiles suggest that non-O157 STEC capable of causing severe human illness are rare in Canadian retail pork, with O157 STEC occasionally occurring. Education regarding the potential risks associated with STEC contamination of pork would be beneficial to help reduce foodborne illnesses.

JOURNAL OF FOOD PROTECTION (2021)

添加到收藏夹

Article Infectious Diseases

Differences in risk factors for transmission among Shiga toxin-producing Escherichia coli serogroups and stx profiles

Gillian A. M. Tarr, Joshua Rounds, Madhura S. Vachon, Kirk Smith, Carlota Medus, Craig W. Hedberg

Summary: This study examines the incidence and risk factors of different serogroups of STEC infections in Minnesota. The results indicate variations in transmission routes and risk factors among different serogroups, suggesting the need for serogroup-specific disease control strategies.

JOURNAL OF INFECTION (2023)

添加到收藏夹

Article Public, Environmental & Occupational Health

An Outbreak of Shiga Toxin-Producing Escherichia coli O157:H7 Associated with a Farming Camp-Tennessee, 2022

Lindsey Ferraro, D. J. Irving, Jack Marr, Kelly Orejuela, Erin Murray, Mugdha Golwalkar, Lisa M. Durso, Julie Viruez, Robin Rasnic, Katie Garman, John Dunn

Summary: On June 22, 2022, the Tennessee Department of Health was informed that a child who attended a farming camp at farm A was hospitalized with Shiga toxin-producing E. coli O157:H7. Three days later, another child who attended the same camp was hospitalized with hemolytic uremic syndrome. This prompted an investigation.

MMWR-MORBIDITY AND MORTALITY WEEKLY REPORT (2023)

添加到收藏夹

Article Microbiology

Analysis of virulence phenotypes and antibiotic resistance in clinical strains of Acinetobacter baumannii isolated in Nashville, Tennessee

Ranashia L. Boone, Briana Whitehead, Tyra M. Avery, Jacky Lu, Jamisha D. Francis, Miriam A. Guevara, Rebecca E. Moore, Schuyler A. Chambers, Ryan S. Doster, Shannon D. Manning, Steven D. Townsend, Leon Dent, Dana Marshall, Jennifer A. Gaddy, Steven M. Damo

Summary: This study analyzed 17 clinical isolates of A. baumannii from diverse anatomical sites and found that variations in biofilm and motility were associated with the anatomical site of isolation. Production of biofilm and hemolysis had an inverse correlation with motility, while motility and hemolysis were negatively correlated with resistance to multiple antibiotics.

BMC MICROBIOLOGY (2021)

添加到收藏夹

Article Immunology

Distinct Group B Streptococcus Sequence and Capsule Types Differentially Impact Macrophage Stress and Inflammatory Signaling Responses

Rebecca A. Flaherty, David M. Aronoff, Jennifer A. Gaddy, Margaret G. Petroff, Shannon D. Manning

Summary: Particularly virulent B group Streptococcus (GBS) ST-17 and CPS III types induce enhanced inflammatory signaling pathway activation and cell death following infection, displaying a stronger ability for internalization and survival.

INFECTION AND IMMUNITY (2021)

添加到收藏夹

Article Multidisciplinary Sciences

Population structure and genetic diversity of non-O157 Shiga toxin-producing Escherichia coli (STEC) clinical isolates from Michigan

Heather M. Blankenship, Rebekah E. Mosci, Stephen Dietrich, Elizabeth Burgess, Jason Wholehan, Karen McWilliams, Karen Pietrzen, Scott Benko, Ted Gatesy, James T. Rudrik, Marty Soehnlen, Shannon D. Manning

Summary: This study used whole genome sequencing to analyze 894 isolates of non-O157 STEC collected from Michigan patients between 2001 and 2018, identifying 67 serotypes and 69 multilocus sequence types. Phylogenetic analysis revealed three clades with evidence of parallel evolution and recombination, showing no specific association between clades and epidemiological data. Serotype diversity increased over time and was associated with infection, hospitalization, and clinical symptoms.

SCIENTIFIC REPORTS (2021)

添加到收藏夹

Article Biochemistry & Molecular Biology

Antibacterial and Anti-biofilm Activity of the Human Breast Milk Glycoprotein Lactoferrin against Group B Streptococcus

Jacky Lu, Jamisha D. Francis, Miriam A. Guevara, Rebecca E. Moore, Schuyler A. Chambers, Ryan S. Doster, Alison J. Eastman, Lisa M. Rogers, Kristen N. Noble, Shannon D. Manning, Steven M. Damo, David M. Aronoff, Steven D. Townsend, Jennifer A. Gaddy

Summary: Group B Streptococcus (GBS) is a pathogen that causes invasive infections in pregnant hosts, neonates, and immunocompromised individuals, while human breast milk lactoferrin has antimicrobial and anti-biofilm activity against GBS.

CHEMBIOCHEM (2021)

添加到收藏夹

Article Microbiology

Antibiotic Susceptibility Profiles and Frequency of Resistance Genes in Clinical Shiga Toxin-Producing Escherichia coli Isolates from Michigan over a 14-Year Period

Sanjana Mukherjee, Heather M. Blankenship, Jose A. Rodrigues, Rebekah E. Mosci, James T. Rudrik, Shannon D. Manning

Summary: The study found an increasing trend in antibiotic resistance frequencies among all STEC serogroups over the past 14 years, especially in non-O157 serogroups. In Michigan, the frequency of ampicillin resistance among O157 isolates was significantly higher than the national average. Genomic analysis revealed 32 distinct antibiotic resistance genes in non-O157 isolates.

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY (2021)

添加到收藏夹

Editorial Material Immunology

A SHHecret target of relapsed medulloblastoma: Astrocytes

Zulekha A. Qadeer, William A. Weiss

Summary: The study reveals that bone morphogenetic proteins and Sox9 promote the transdifferentiation of tumor cells into tumor-derived astrocytes, which can be targeted by BMP inhibitors.

JOURNAL OF EXPERIMENTAL MEDICINE (2021)

添加到收藏夹

Article Food Science & Technology

Variability in the Occupancy of Escherichia coli O157 Integration Sites by Shiga Toxin-Encoding Prophages

Scott T. Henderson, Pallavi Singh, David Knupp, David W. Lacher, Galeb S. Abu-Ali, James T. Rudrik, Shannon D. Manning

Summary: In this study, PCR assays were used to examine the occupancy of Shiga toxin prophages in different loci among 346 E. coli O157 strains, revealing variations in occupancy profiles among different clades. Strains belonging to clade 8 were found to have unique prophage occupancy profiles that could impact toxin expression, providing insights into the complexity of prophage integration and its implications on virulence.

TOXINS (2021)

添加到收藏夹

Article Immunology

Analysis of Susceptibility to the Antimicrobial and Anti-Biofilm Activity of Human Milk Lactoferrin in Clinical Strains of Streptococcus agalactiae With Diverse Capsular and Sequence Types

Jacky Lu, Miriam A. Guevara, Jamisha D. Francis, Sabrina K. Spicer, Rebecca E. Moore, Schuyler A. Chambers, Kelly M. Craft, Shannon D. Manning, Steven D. Townsend, Jennifer A. Gaddy

Summary: This study demonstrates that lactoferrin possesses antimicrobial and antibiofilm properties against a wide range of GBS isolates, with maternally isolated colonizing strains being the most susceptible.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2021)

添加到收藏夹

Article Microbiology

The antimicrobial activity of zinc against group B Streptococcus is strain-dependent across diverse sequence types, capsular serotypes, and invasive versus colonizing isolates

Jamisha D. Francis, Miriam A. Guevara, Jacky Lu, Shabir A. Madhi, Gaurav Kwatra, David M. Aronoff, Shannon D. Manning, Jennifer A. Gaddy

Summary: This study found that the susceptibility or resistance to zinc intoxication in Streptococcus agalactiae strains varies based on their isolation source, capsular serotype, and sequence type. Invasive strains are less susceptible to zinc toxicity compared to colonizing strains. Moreover, capsular type III strains and the ST-17 and ST-19 strains exhibit the greatest resilience to zinc stress.

BMC MICROBIOLOGY (2022)

添加到收藏夹

Article Biochemistry & Molecular Biology

Galacto-Oligosaccharide Supplementation Modulates Pathogen-Commensal Competition between Streptococcus agalactiae and Streptococcus salivarius

Rebecca E. Moore, Harrison C. Thomas, Shannon D. Manning, Jennifer A. Gaddy, Steven D. Townsend

Summary: The members of infant microbiome are influenced by feeding method and prebiotics, which can affect the interactions between commensals and pathogens. The study provides insights into how single species survive in the gut, the niche they occupy, and their interactions with other members of the microbiota.

CHEMBIOCHEM (2022)

添加到收藏夹

Article Chemistry, Medicinal

Antibiofilm Activity of Human Milk Oligosaccharides against Multidrug Resistant and Susceptible Isolates of Acinetobacter baumannii

Sabrina K. Spicer, Rebecca E. Moore, Jacky Lu, Miriam A. Guevara, Dana R. Marshall, Shannon D. Manning, Steven M. Damo, Steven D. Townsend, Jennifer A. Gaddy

Summary: A. baumannii poses a serious threat to human health by forming biofilms that lead to difficult-to-treat infections. Human milk oligosaccharides (HMOs) exhibit strong antibiofilm properties and are effective against most strains of A. baumannii.

ACS INFECTIOUS DISEASES (2021)

添加到收藏夹

Article Microbiology

Nitric Oxide Induced stx2 Expression Is Inhibited by the Nitric Oxide Reductase, NorV, in a Clade 8 Escherichia coli O157:H7 Outbreak Strain

Rim Al Safadi, Michelle L. Korir, Shannon D. Manning

Summary: The virulence of Escherichia coli O157:H7 is influenced by a specific gene, norV, which affects toxin production. Variation in norV function and the impact of oxygen and nitric oxide (NO) on toxin production differ among different strains. This study contributes to understanding the pathogenic mechanisms of this strain.

MICROORGANISMS (2022)

添加到收藏夹

Article Microbiology

Zoonotic Transmission of Campylobacter jejuni to Caretakers From Sick Pen Calves Carrying a Mixed Population of Strains With and Without Guillain Barre Syndrome-Associated Lipooligosaccharide Loci

Jessica L. St. Charles, Phillip T. Brooks, Julia A. Bell, Husnain Ahmed, Mia Van Allen, Shannon D. Manning, Linda S. Mansfield

Summary: This study found a close relationship between C. jejuni strains from sick calves and those infecting family members, suggesting zoonotic transmission. Additionally, despite repeated exposure to C. jejuni strains with LOS known to elicit autoimmune reactions, the family members did not develop GBS.

FRONTIERS IN MICROBIOLOGY (2022)

添加到收藏夹

Article Multidisciplinary Sciences

Streptococcus agalactiae cadD alleviates metal stress and promotes intracellular survival in macrophages and ascending infection during pregnancy

Michelle L. Korir, Ryan S. Doster, Jacky Lu, Miriam A. Guevara, Sabrina K. Spicer, Rebecca E. Moore, Jamisha D. Francis, Lisa M. Rogers, Kathryn P. Haley, Amondrea Blackman, Kristen N. Noble, Alison J. Eastman, Janice A. Williams, Steven M. Damo, Kelli L. Boyd, Steven D. Townsend, C. Henrique Serezani, David M. Aronoff, Shannon D. Manning, Jennifer A. Gaddy

Summary: This study reveals that the gene cadD in Group B Streptococcus (GBS) enhances metal resistance in macrophages, reducing metal toxicity and promoting bacterial proliferation in the pregnant host. Deletion of cadD decreases GBS survival in macrophages and reduces invasion in gestational tissues.

NATURE COMMUNICATIONS (2022)

添加到收藏夹

Meeting Abstract Obstetrics & Gynecology

A Model of Long-Term Group B Streptococcus Vaginal Colonization and Subsequent Pregnancy

Kelly M. Collins, Cole McCutcheon, Shannon D. Manning, Margaret Petroff

REPRODUCTIVE SCIENCES (2022)

添加到收藏夹

暂无数据

© Peeref 2019-2024. All rights reserved.