Article
Multidisciplinary Sciences
Kathleen Baird, Halima Said, Hendrik J. Koornhof, Adriano Gianmaria Duse
Summary: Background: Correctional centres in South Africa have high rates of tuberculosis (TB) transmission and disease progression. This study aimed to assess the TB diagnosis and treatment among detainees in a South African correctional centre.
Methods: A prospective observational study was conducted from February to September 2017, enrolling adult male detainees with positive TB screening tests. Sputum samples underwent smear microscopy and culture, with strain typing performed on culture-positive samples.
Results: During the study period, 130 TB cases were detected through routine admission screening, and 102 eligible male detainees were enrolled in the study. All TB cases were detected within 30 hours of admission screening, and the majority of participants started treatment within 48 hours of TB diagnosis. However, only 8% of participants completed treatment at the correctional centre. The most frequent lineage of Mycobacterium tuberculosis strains was the Beijing family.
Conclusion: The current TB control efforts in the correctional centre showed strengths in rapid case detection and prompt treatment initiation. However, there is a need to improve the completion rate of TB treatment by strengthening the linkage between correctional TB services and community TB services after detainees are released to prevent TB transmission.
Article
Immunology
Zofia Bakula, Mateusz Marczak, Agata Bluszcz, Malgorzata Proboszcz, Justyna Kosciuch, Rafal Krenke, Petras Stakenas, Igor Mokrousov, Tomasz Jagielski
Summary: This study investigated the genetic diversity of Mycobacterium tuberculosis isolates in Poland, with a focus on multidrug-resistant strains. The results showed that the Beijing genotype was the most prevalent among MDR-TB cases, especially among foreign-born patients, indicating possible transmission from FSU countries. These findings are important for monitoring and controlling the spread of tuberculosis in Poland.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Alejandro Vega Marin, Nalin Rastogi, David Couvin, Viviana Mape, Martha Isabel Murcia
Summary: Tuberculosis affects vulnerable groups, including indigenous populations. A study in Colombia used genotyping to determine molecular epidemiology of clinical isolates in indigenous communities, finding diversity in genotypes and potential active transmission chains.
Article
Microbiology
S. M. Mazidur Rahman, Arfatur Rahman, Rumana Nasrin, Md Fahim Ather, Sara Sabrina Ferdous, Shahriar Ahmed, Mohammad Khaja Mafij Uddin, Razia Khatun, Mohammad Shahnewaz Sarker, Asif Mujtaba Mahmud, Md Mojibur Rahman, Sayera Banu
Summary: This study characterized the MDR tuberculosis isolates circulating in Bangladesh and investigated their mode of transmission. The majority of the MDR M. tuberculosis isolates were of the modern virulent type, with the Beijing and T lineages being the predominant types and implicated in the majority of MDR-TB transmission. The findings suggest that the emergence of MDR-TB in Bangladesh is largely due to acquired resistance, emphasizing the importance of rapid diagnosis and adherence to appropriate treatment.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Ahmed Gaballah, Abeer Ghazal, Reda Almiry, Somaya Hussein, Rasha Emad, Eglal El-Sherbini
Summary: This study aimed to evaluate the transmission patterns of Mycobacterium tuberculosis strains in Alexandria, Egypt using MIRU-VNTR genotyping technique. The results revealed a low clustering rate and a low recent transmission rate of M. tuberculosis strains. Additionally, the S family was found to be the dominant lineage among the isolates.
JOURNAL OF MEDICAL MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Elizabeth Hortencio de Melo, Harrison Magdinier Gomes, Philip Noel Suffys, Marcia Quinhones Pires Lopes, Raquel Lima de Figueiredo Teixeira, Icaro Rodrigues dos Santos, Marilia Masello Junqueira Franco, Helio Langoni, Antonio Carlos Paes, Jose Augusto Bastos Afonso, Carla Lopes de Mendonca
Summary: This study characterized the genetic variability of Mycobacterium bovis causing bovine tuberculosis in Pernambuco, Brazil for the first time, showing strain diversity in the state. Loci ETR-A had higher discriminatory power, while loci ETR-B, ETR-C, MIRU16, MIRU27, and QUB26 showed moderate allelic diversity.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Pejman Bakhtiyariniya, Azar Dokht Khosravi, Mohammad Hashemzadeh, Mohammad Savari
Summary: This study investigated the genetic diversity of MTB isolates resistant to first-line anti-tuberculosis drugs using 24-locus MIRU-VNTR in southwestern Iran. The results showed a high degree of genetic diversity among drug-resistant MTB isolates, with the recommendation of using 15-locus MIRU-VNTR for preliminary genotyping.
Article
Multidisciplinary Sciences
Kangjam Rekha Devi, Jagat Pradhan, Rinchenla Bhutia, Peggy Dadul, Atanu Sarkar, Nitumoni Gohain, Kanwar Narain
Summary: This study explores the molecular diversity of the Mycobacterium tuberculosis complex in Sikkim, India, where the Beijing spoligotype dominates and is associated with Multiple Drug Resistance. By using genetic characterization and machine learning methods, high accuracy in predicting different spoligotypes was achieved.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Sadiq Mohammed Damina, David Atomanyi Barnes, Bitrus Inuwa, Gulak Hussaini Ularamu, Mohammed Bello, Olu Solomon Okaiyeto, Ayuba Caleb Kudi, Jeewan Thapa, Chie Nakajima, Yasuhiko Suzuki
Summary: Despite minimal enforcement of control measures in Nigeria, it was found that 183 out of 13,688 slaughtered cattle in Adamawa and Gombe States had tuberculosis lesions. This study emphasizes the need for enhanced research and surveillance, and proposes a cost-effective approach to understand the molecular epidemiology of bovine tuberculosis in Nigeria.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Immunology
Michelle Li Wei Kam, Leo K. Y. Lim, Li-Hwei Sng, Rick Twee-Hee Ong, Vernon J. M. Lee, Yee Tang Wang, Cynthia B. E. Chee
Summary: This study describes the molecular epidemiology of Mycobacterium tuberculosis complex (MTBC) and factors associated with its transmission in Singapore. The results showed that the East-Asian Lineage 2 strain played a significant role in TB transmission and clustered cases had specific demographic and clinical characteristics. This has important implications for TB control strategies.
Article
Infectious Diseases
Chunjie Yin, Xiaokaiti Mijiti, Haican Liu, Quan Wang, Bin Cao, Aiketaguli Anwaierjiang, Machao Li, Mengwen Liu, Yi Jiang, Miao Xu, Kanglin Wan, Xiuqin Zhao, Guilian Li, Hui Xiao
Summary: This study investigated the molecular epidemiological characteristics and drug resistance of Mycobacterium tuberculosis in southern Xinjiang, China. The Beijing genotype was found to be the dominant lineage, associated with drug resistance, more likely to infect young people, and contribute to tuberculosis transmission.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Microbiology
Ranjitha Shankaregowda, Yuan Hu Allegretti, Mahadevaiah Neelambike Sumana, Morubagal Raghavendra Rao, Eva Raphael, Padukudru Anand Mahesh, Lee W. Riley
Summary: The association between tuberculosis and type 2 diabetes mellitus is a challenge in managing the convergence of these epidemics. Our study aimed to determine the diversity of Mycobacterium tuberculosis genotypes in patients with and without type 2 diabetes.
Review
Parasitology
Emilyn Costa Conceicao, Richard Steiner Salvato, Karen Machado Gomes, Arthur Emil dos Santos Guimaraes, Marilia Lima da Conceicao, Ricardo Jose de Paula Souza e Guimaraes, Abhinav Sharma, Ismari Perini Furlaneto, Regina Bones Barcellos, Valdes Roberto Bollela, Livia Maria Pala Anselmo, Maria Carolina Sisco, Cristina Viana Niero, Lucilaine Ferrazoli, Guislaine Refregier, Maria Cristina da Silva Lourenco, Harrison Magdinier Gomes, Artemir Coelho de Brito, Marcos Catanho, Rafael Silva Duarte, Philip Noel Suffys, Karla Valeria Batista Lima
Summary: Molecular typing can be helpful in studying tuberculosis transmission, which is significant for improving TB control strategies and establishing a database.
MEMORIAS DO INSTITUTO OSWALDO CRUZ
(2021)
Article
Infectious Diseases
Thoko Flav Kapalamula, Joseph Chizimu, Lawrence Belotindos, Mwangala Akapelwa, Dipti Shrestha, Mirriam Ethel Nyenje, Musso Munyeme, Bernard Mudenda Hang'ombe, Rajhab Sawasawa Mkakosya, Stephen V. Gordon, Chie Nakajima, Yasuhiko Suzuki
Summary: Bovine tuberculosis is a neglected disease that has a higher burden in developing countries due to lack of control measures, such as testing and slaughter of infected cattle and pasteurization of milk. Molecular typing studies of M. bovis isolates from a cold storage abattoir in Malawi revealed high diversity of the pathogen in the area, suggesting long-term circulation of the infection. Genetic similarities between isolates from Malawi, Zambia, and Mozambique indicate transmission links in this region and highlight the need for improved control strategies.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Infectious Diseases
Mostafa Naseri-Nezhad, Mahla Asadian, Mohammad Khalifeh Gholi, Mehdi Yaseri, Masoumeh Douraghi
Summary: This study used 24-locus MIRU-VNTR genotyping to identify the circulating Mycobacterium tuberculosis genotypes in Qom province. The results showed that about half of the isolates belonged to Afghan immigrants, which poses a risk to the situation of tuberculosis in Qom. The similarity between Afghan and Iranian genotypes provides evidence for the involvement of immigrants in the transmission of M. tuberculosis. This study is important for understanding the prevalence and distribution of M. tuberculosis genotypes, their association with risk factors, and the impact of immigration on the situation of tuberculosis in Qom province.
BMC INFECTIOUS DISEASES
(2023)
Article
Genetics & Heredity
Doctor B. Sibandze, Alexander Kay, Viola Dreyer, Welile Sikhondze, Qiniso Dlamini, Andrew DiNardo, Godwin Mtetwa, Bhekumusa Lukhele, Debrah Vambe, Christoph Lange, Muyalo Glenn Dlamini, Tara Ness, Rojelio Mejia, Barbara Kalsdorf, Jan Heyckendorf, Martin Kuhns, Florian P. Maurer, Sindisiwe Dlamini, Gugu Maphalala, Stefan Niemann, Anna Mandalakas
Summary: Stool is an important diagnostic specimen for tuberculosis, especially in populations unable to provide sputum. However, conventional culture methods perform poorly in isolating M. tuberculosis complex strains from stool samples, limiting drug resistance testing. This study demonstrates that targeted next-generation sequencing (tNGS) can effectively detect drug resistance mutations from stool specimens, providing critical diagnostic information for tuberculosis patients who struggle to produce sputum samples.
Article
Microbiology
Nicolas Gisch, Christian Utpatel, Lisa M. Gronbach, Thomas A. Kohl, Ursula Schombel, Sven Malm, Karen M. Dobos, Danny C. Hesser, Roland Diel, Udo Goetsch, Silke Gerdes, Yassir A. Shuaib, Nyanda E. Ntinginya, Celso Khosa, Sofia Viegas, Glennah Kerubo, Solomon Ali, Sahal A. Al-Hajoj, Perpetual W. Ndung'u, Andrea Rachow, Michael Hoelscher, Florian P. Maurer, Dominik Schwudke, Stefan Niemann, Norbert Reiling, Susanne Homolka
Summary: Using whole genome sequencing, we characterized the global population structure of Lineage 1 (L1) strains of Mycobacterium tuberculosis complex (MTBC) and correlated this with the synthesis of phenolic glycolipids (PGL) - known virulence factors. Our results revealed eight major L1 sub-lineages with specific mutation signatures in PGL biosynthesis genes. Furthermore, we found that strains with abolished PGL biosynthesis showed enhanced growth in human macrophages.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Ludovic Meunier, Matthieu Godin, Geraud Souteyrand, Benoit Mottin, Yann Valy, Vincent Lordet, Christian Benoit, Ronan Bakdi, Virginie Laurencon, Philippe Genereux, Matthias Waliszewski, Caroline Allix-Beguec
Summary: We evaluated a decision algorithm for percutaneous coronary interventions (PCI) based on a no-stent strategy and found it to be effective and safe. The combination of scoring balloon angioplasty (SCBA) and drug-coated balloon (DCB) was the preferred approach. The incidence of major adverse cardiac events (MACE) was low during the one-year follow-up period.
CLINICAL RESEARCH IN CARDIOLOGY
(2023)
Correction
Cardiac & Cardiovascular Systems
Ludovic Meunier, Matthieu Godin, Geraud Souteyrand, Benoit Mottin, Yann Valy, Vincent Lordet, Christian Benoit, Ronan Bakdi, Virginie Laurencon, Philippe Genereux, Matthias Waliszewski, Caroline Allix-Beguec
CLINICAL RESEARCH IN CARDIOLOGY
(2023)
Article
Infectious Diseases
Jabar Babatunde Pacome Achimi Agbo Abdul, Bayode Romeo Adegbite, Micheska Epola Dibamba Ndanga, Jean Ronald Edoa, Rhett Chester Mevyann, Guy Rogue Arnault Ibinda Mfoumbi, Tshisekedi Jean de Dieu, Jocelyn Mahoumbou, Christopher Mebiame Biyogho, Sankarganesh Jeyaraj, Stefan Niemann, Bertrand Lell, Peter Gottfried Kremsner, Abraham Sunday Alabi, Ayola Akim Adegnika, Martin Peter Grobusch
Summary: The increasing incidence of MDR-TB infection in Gabon is alarming, especially among individuals aged 25-35. There is a need for improvement in case finding and contact tracing strategies for treatment-naive MDR-TB patients.
Editorial Material
Infectious Diseases
Annelies Van Rie, Timothy Walker, Bouke de Jong, Praharshinie Rupasinghe, Emmanuel Riviere, Veronique Dartois, Lindsay Sonnenkalb, Diana Machado, Sebastien Gagneux, Philip Supply, Viola Dreyer, Stefan Niemann, Galo Goig, Conor Meehan, Elisa Tagliani, Daniela Maria Cirillo
LANCET INFECTIOUS DISEASES
(2022)
Article
Multidisciplinary Sciences
Matthias Merker, Jean-Philippe Rasigade, Maxime Barbier, Helen Cox, Silke Feuerriegel, Thomas A. Kohl, Egor Shitikov, Kadri Klaos, Cyril Gaudin, Rudy Antoine, Roland Diel, Sonia Borrell, Sebastien Gagneux, Vladyslav Nikolayevskyy, Soenke Andres, Valeriu Crudu, Philip Supply, Stefan Niemann, Thierry Wirth
Summary: The research revealed that the W148 strain originated around 1963, and subsequently underwent two epidemic expansions in the late 20th century, developing resistance to multiple anti-TB drugs. The study also found that this strain carries a variety of drug resistance genes, posing a threat to the successful introduction of new anti-M/XDR-TB antibiotic regimens.
NATURE COMMUNICATIONS
(2022)
Letter
Public, Environmental & Occupational Health
Jonathan P. Smith, Chawangwa Modongo, John E. Oeltmann, Mbatshi Dima, Ogopotse Matsiri, Othusitse Fane, Tudeutso Molefi, Sanghyuk S. Shin, Ivan Barilar, Stefan Niemann, Nicola M. Zetola, Patrick K. Moonan
AMERICAN JOURNAL OF EPIDEMIOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Roland Diel, Karen Meywald-Walter, Christian Schwarzbach, Klaas Voss, Viola Dreyer, Stefan Niemann
Summary: A prospective molecular-epidemiologic study in Hamburg, Germany, evaluated the transmission risk of Mycobacterium tuberculosis complex (Mtbc) by children <15 years in a low-incidence setting. The study found that children with pulmonary TB disease, especially those aged below 10 years, rarely transmit Mtbc to their close contacts in a low-incidence setting.
RESPIRATORY MEDICINE
(2023)
Article
Microbiology
Rachel F. Rollo, Giorgia Mori, Timothy A. Hill, Doris Hillemann, Stefan Niemann, Susanne Homolka, David P. Fairlie, Antje Blumenthal
Summary: Shorter and more effective treatment regimens as well as new drugs are urgently needed to reduce the global burden of tuberculosis (TB). This study discovered a new compound called wollamide B1, which has antimycobacterial activity and can synergize with existing TB antibiotics, enhancing their effectiveness. Wollamide B1 also remains effective against drug-resistant TB strains.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Francy J. Perez-Llanos, Viola Dreyer, Ivan Barilar, Christian Utpatel, Thomas A. Kohl, Martha Isabel Murcia, Susanne Homolka, Matthias Merker, Stefan Niemann
Summary: An outbreak of Mycobacterium tuberculosis complex (MTBC) bacteria was identified among the indigenous populations in the Colombian Amazon region. Whole genome sequencing (WGS) was used to analyze the transmission of MTBC strains and it was found that the use of an alternative reference genome and repetitive regions improved the phylogenetic resolution. The study also revealed the presence of multiple clones in some patients, indicating within-host MTBC diversity.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Lindsay Sonnenkalb, Joshua James Carter, Andrea Spitaleri, Zamin Iqbal, Martin Hunt, Kerri Marie Malone, Christian Utpatel, Daniela Maria Cirillo, Camilla Rodrigues, Kayzad Soli Nilgiriwala, Philip William Fowler, Matthias Merker, Stefan Niemann
Summary: This study identified genetic variations that confer resistance to bedaquiline and clofazimine, and established a mutation catalogue using experimental evolution, protein modelling, genome sequencing, and phenotypic data analysis. The findings advance the understanding of drug resistance mechanisms in Mycobacterium tuberculosis complex strains and provide important genetic testing evidence for the design of effective treatments.
Article
Microbiology
Raphael Gries, Michael Dal Molin, Jason Chhen, Edeltraud van Gumpel, Viola Dreyer, Stefan Niemann, Jan Rybniker
Summary: Drug-resistant tuberculosis is a global health care threat that requires new and effective treatment options. Two novel cytochrome bc(1) inhibitors (MJ-22 and B6) have been identified as effective in targeting the Mycobacterium tuberculosis respiratory chain in human macrophages. These compounds have shown low mutation frequencies and unique cross-resistance patterns compared to other advanced cytochrome bc(1) inhibitors.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Cardiac & Cardiovascular Systems
Antoine Milhem, Caroline Allix-Beguec, Virginie Laurencon, Frederic Treguer, Olivier Cesari
Summary: Analyzing changes in the pressure curve can monitor the adhesion between the cryoballoon and the pulmonary vein, which is a simple and efficient method to detect balloon movement during the early stages of treatment.
JOURNAL OF ARRHYTHMIA
(2023)