Article
Microbiology
Guillaume Girault, Ludivine Perrot, Virginie Mick, Claire Ponsart
Summary: In this study, a high-resolution melting (HRM) method was developed for the rapid identification and classification of the Brucella genus based on 17 specific single nucleotide polymorphisms (SNPs) and SNP analysis. This genotyping tool provides a new and quick alternative for Brucella identification, significantly saving time for the diagnosis of brucellosis.
Article
Chemistry, Analytical
Robert S. Plumb, Thomas McDonald, Paul D. Rainville, Jason Hill, Lee A. Gethings, Kelly A. Johnson, Ian D. Wilson
Summary: Vacuum jacketed column (VJC) technology can significantly improve the performance of UHPLC systems, enhancing chromatographic separation and increasing detection capabilities for metabolites. Using the VJC system can reduce peak tailing, increase peak capacity, and enhance the intensity and response of MS peaks.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Vasyl Yatsyna, Ali H. Abikhodr, Ahmed Ben Faleh, Stephan Warnke, Thomas R. Rizzo
Summary: Coupling vibrational ion spectroscopy with high-resolution ion mobility separation allows for detailed analysis of biomolecules in the gas phase. A novel multiplexed approach using Hadamard transform multiplexing is introduced, which improves the signal-to-noise ratio of infrared spectra compared to conventional methods. This study demonstrates the utility of this approach for cryogenic ion spectroscopy of peptides and glycans in mixtures.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Patrick Leopold Ruther, Immanuel Mirnes Husic, Pernille Bangsgaard, Kristian Murphy Gregersen, Pernille Pantmann, Milena Carvalho, Ricardo Miguel Godinho, Lukas Friedl, Joao Cascalheira, Alberto John Taurozzi, Marie Louise Schjellerup Jorkov, Michael M. Benedetti, Jonathan Haws, Nuno Bicho, Frido Welker, Enrico Cappellini, Jesper Velgaard Olsen
Summary: The authors developed a workflow called Species by Proteome INvestigation (SPIN) for analyzing archaeological bone using liquid chromatography-tandem mass spectrometry, which is capable of identifying over 150 mammalian species with automated sample preparation and faster data acquisition. The SPIN method was successfully validated in both known reference bones and blind tests of Iron-Age material from Scandinavia, demonstrating its reproducibility and high throughput capabilities. This new method has the potential to expand to other biological tissues and taxa in the future.
NATURE COMMUNICATIONS
(2022)
Review
Plant Sciences
Robert D. Hall, John C. D'Auria, Antonio C. Silva Ferreira, Yves Gibon, Dariusz Kruszka, Puneet Mishra, Rick van de Zedde
Summary: High-throughput plant phenotyping approaches are rapidly developing and helping bridge the genotype-phenotype gap. Metabolites play a crucial role in defining key physiological and agronomic traits in plants. Despite challenges, novel technological innovations have the potential to fully integrate metabolomics approaches into plant phenotyping pipelines in the near future.
TRENDS IN PLANT SCIENCE
(2022)
Article
Chemistry, Analytical
Disheng Feng, Hang Li, Tianrun Xu, Fujian Zheng, Chunxiu Hu, Xianzhe Shi, Guowang Xu
Summary: The study presents a method for high-throughput single cell metabolomics analysis using a serpentine channel microfluidic device coupled with pulsed electric field-induced electrospray ionization-high resolution mass spectrometry (PEF-ESI-HRMS). The method allows for the analysis of single cells under near-physiological conditions and has successfully discriminated different cancer cells based on their individual metabolic profiles.
ANALYTICA CHIMICA ACTA
(2022)
Article
Multidisciplinary Sciences
Xiaoyu Zhou, Zhuofan Wang, Jingjin Fan, Zheng Ouyang
Summary: Ion mobility is utilized in mass spectrometers for analyzing the structure of biomolecules. Researchers demonstrate that ion mobility analysis in an ion trap with ultra-high fields can achieve resolutions over 10,000 for isomer separation, as shown for disaccharide, phospholipid, and peptide isomers. Elucidating complex structures of biomolecules is crucial in chemistry and life sciences. In the last decade, ion mobility coupled with mass spectrometry has become a unique tool for distinguishing isomers and isoforms of biomolecules. This study presents a concept for ion mobility analysis using an ion trap, enabling ultra-high field isomer separation and achieving resolutions over 10,000. The potential of this technology is demonstrated for analyzing isomers of disaccharides, phospholipids, and peptides with post-translational modifications.
NATURE COMMUNICATIONS
(2023)
Article
Biochemical Research Methods
Julien Slagboom, Rico J. E. Derks, Raya Sadighi, Govert W. Somsen, Chris Ulens, Nicholas R. Casewell, Jeroen Kool
Summary: In this study, the authors developed a new analytical strategy called high-throughput (HT) venomics, capable of performing a full proteomic analysis of snake venom in a short time. The strategy involves RP-HPLC-nanofractionation analytics, mass spectrometry analysis, in-solution tryptic digestion, and high-throughput proteomics. The authors also developed scripts to process and analyze the obtained data. They applied this strategy to venom samples from various medically important snake species and found it to be a valuable tool for studying venom variation.
JOURNAL OF PROTEOME RESEARCH
(2023)
Review
Chemistry, Analytical
Vera Plekhova, Kimberly De Windt, Margot De Spiegeleer, Marilyn De Graeve, Lynn Vanhaecke
Summary: Biofluid metabolomics is a valuable tool for discovering biomarkers related to disease, genetics, and exposure. Direct infusion and ambient ionization mass spectrometry offer attractive alternatives for rapid analysis with minimal sample handling.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Monika Borkowska, Ewelina Celinska
Summary: Yeasts play an important role in food production, and reliable methods for yeast identification and differentiation are needed to monitor production processes and ensure the authenticity and safety of foods. In this study, a qPCR-HRM protocol based on three targeted regions was developed for rapid differentiation of yeast species isolated from food products. The simultaneous analysis of the three regions resulted in rapid and accurate differentiation of all the yeast strains at species-level resolution.
Article
Biochemistry & Molecular Biology
Marta Tomczyk, Mariola Olkowicz, Ewa M. Slominska, Ryszard T. Smolenski
Summary: The heart has a flexible energy metabolism and can utilize various carbon substrates, with substrate preference potentially impacting cardiac pathologies. A simple method was developed to analyze cardiac metabolic substrate use, allowing for fast estimation of glucose and amino acid utilization in mice and high-throughput analysis of changes in pathology and after pharmacological treatments.
Article
Chemistry, Analytical
Brandon Y. Lieng, Andrew T. Quaile, Xavier Domingo-Almenara, Hannes L. Rost, J. Rafael Montenegro-Burke
Summary: In MS-based untargeted metabolomics, the identification of some metabolites is challenging due to the uncertainties in resolving isobars and structurally similar species. To address this issue, MSn fragmentation techniques can be applied to probe metabolites for additional structural information. However, the lack of high-resolution-MSn spectra in spectral libraries limits the accuracy of metabolite identification. In this study, a computational approach was developed to generate a database of high-resolution-MSn spectra by converting existing low-resolution-MSn spectra.
ANALYTICAL CHEMISTRY
(2023)
Article
Environmental Sciences
A. Ruth Godfrey, Jonathan Dunscombe, Anthony Gravell, Ann Hunter, Mark P. Barrow, Geertje van Keulen, Claire Desbrow, Rachel Townsend
Summary: The study demonstrates that the modified QuEChERS sample preparation method is a viable option for efficient and cost-effective monitoring of environmental pollutants. The method offers reduced extraction times, good reproducibility, and the ability to differentiate between different samples. It also identifies new potential pollutant candidates.
Article
Immunology
Amol O. Bajaj, Suraj Saraswat, Juha E. A. Knuuttila, Joanna Freeke, J. Benjamin Stielow, Adam P. Barker
Summary: This study demonstrates the successful application of a novel mass spectrometry-based method in identifying clinically relevant Mycobacterium tuberculosis complex species that are very closely related and difficult to differentiate with current methods. By analyzing protein profiles, the method shows the potential for rapid and accurate identification of these species.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Qi Xiao, Fangfei Zhang, Luang Xu, Liang Yue, Oi Lian Kon, Yi Zhu, Tiannan Guo
Summary: This paper summarizes the advances and limitations of biomarkers in cancer research, as well as the important applications of mass spectrometry technology in proteomics. The article emphasizes the role of artificial intelligence technology in clinical studies, as well as the importance of combining computational and statistical methods in biomarker research.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Article
Immunology
Scott R. Evans, Andrea M. Hujer, Hongyu Jiang, Kristine M. Hujer, Thomas Hall, Christine Marzan, Michael R. Jacobs, Rangarajan Sampath, David J. Ecker, Claudia Manca, Kalyan Chavda, Pan Zhang, Helen Fernandez, Liang Chen, Jose R. Mediavilla, Carol B. Hill, Federico Perez, Angela M. Caliendo, Vance G. Fowler, Henry F. Chambers, Barry N. Kreiswirth, Robert A. Bonomo
CLINICAL INFECTIOUS DISEASES
(2016)
Article
Critical Care Medicine
Jean-Louis Vincent, David Brealey, Nicolas Libert, Nour Elhouda Abidi, Michael O'Dwyer, Kai Zacharowski, Malgorzata Mikaszewska-Sokolewicz, Jacques Schrenzel, Francois Simon, Mark Wilks, Marcus Picard-Maureau, Donald B. Chalfin, David J. Ecker, Rangarajan Sampath, Mervyn Singer
CRITICAL CARE MEDICINE
(2015)
Correction
Agriculture, Dairy & Animal Science
Vincent Perreten, Andrea Endimiani, Andreas Thomann, Juliette R. K. Wipf, Alexandra Rossando, Michele Bodmer, Andreas Raemy, Kristin A. Sannes-Lowery, David J. Ecker, Rangarajan Sampath, Robert A. Bonomo, Cicely Washington
JOURNAL OF DAIRY SCIENCE
(2015)
Article
Multidisciplinary Sciences
Mark W. Eshoo, Heather E. Carolan, Christian Massire, Danny M. Chou, Chris D. Crowder, Megan A. Rounds, Curtis A. Phillipson, Steven E. Schutzer, David J. Ecker
Article
Medicine, General & Internal
Catherine Satzke, Eileen M. Dunne, Barbara D. Porter, Keith P. Klugman, E. Kim Mulholland
Article
Cell Biology
Andrew J. Kennedy, Elizabeth J. Rahn, Brynna S. Paulukaitis, Katherine E. Savell, Holly B. Kordasiewicz, Jing Wang, John W. Lewis, Jessica Posey, Sarah K. Strange, Mikael C. Guzman-Karlsson, Scott E. Phillips, Kyle Decker, S. Timothy Motley, Eric E. Swayze, David J. Ecker, Todd P. Michael, Jeremy J. Day, J. David Sweatt
Article
Microbiology
Andrea Bacconi, Gregory S. Richmond, Michelle A. Baroldi, Thomas G. Laffler, Lawrence B. Blyn, Heather E. Carolan, Mark R. Frinder, Donna M. Toleno, David Metzgar, Jose R. Gutierrez, Christian Massire, Megan Rounds, Natalie J. Kennel, Richard E. Rothman, Stephen Peterson, Karen C. Carroll, Teresa Wakefield, David J. Ecker, Rangarajan Sampath
JOURNAL OF CLINICAL MICROBIOLOGY
(2014)
Article
Virology
Jiazhen Chen, Yongfeng Fu, Liwen Ju, Xinyu Miao, Yaojie Shen, Lei He, Wenjie Wang, Jialin Jin, Lingyun Shao, Rangarajan Sampath, David J. Ecker, Ying Zhang, Min Li, Xunjia Cheng, Wenhong Zhang
JOURNAL OF CLINICAL VIROLOGY
(2014)
Article
Multidisciplinary Sciences
David Metzgar, Mark W. Frinder, Richard E. Rothman, Stephen Peterson, Karen C. Carroll, Sean X. Zhang, Gideon D. Avornu, Megan A. Rounds, Heather E. Carolan, Donna M. Toleno, David Moore, Thomas A. Hall, Christian Massire, Gregory S. Richmond, Jose R. Gutierrez, Rangarajan Sampath, David J. Ecker, Lawrence B. Blyn
Article
Microbiology
Michael R. Mosel, Heather E. Carolan, Alison W. Rebman, Steven Castro, Christian Massire, David J. Ecker, Mark J. Soloski, John N. Aucott, Mark W. Eshoo
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2019)
Article
Medicine, Research & Experimental
Shane G. Poplawski, Krassimira A. Garbett, Rebekah L. McMahan, Holly B. Kordasiewicz, Hien Zhao, Andrew J. Kennedy, Slavina B. Goleva, Teresa H. Sanders, S. Timothy Motley, Eric E. Swayze, David J. Ecker, J. David Sweatt, Todd P. Michael, Celeste B. Greer
MOLECULAR THERAPY-NUCLEIC ACIDS
(2020)
Article
Microbiology
Michael R. Mosel, Alison W. Rebman, Heather E. Carolan, Tristan Montenegro, Robert Lovari, Steven E. Schutzer, David J. Ecker, Ting Yang, Nitya S. Ramadoss, William H. Robinson, Mark J. Soloski, Mark W. Eshoo, John N. Aucott
Summary: The study conducted on Lyme disease patients showed that PCR-based direct detection methods can help differentiate patient subgroups, especially those who are serologically negative in the early stages of infection. This approach could be particularly useful for future vaccine trials and public health surveillance of tick-borne diseases.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Microbiology
Vaclav Honig, Heather E. Carolan, Zuzana Vavruskova, Christian Massire, Michael R. Mosel, Christopher D. Crowder, Megan A. Rounds, David J. Ecker, Daniel Ruzek, Libor Grubhoffer, Benjamin J. Luft, Mark W. Eshoo
FEMS MICROBIOLOGY ECOLOGY
(2017)
Article
Infectious Diseases
M. J. O'Dwyer, M. H. Starczewska, J. Schrenzel, K. Zacharowski, D. J. Ecker, R. Sampath, D. Brealey, M. Singer, N. Libert, M. Wilks, J. -L. Vincent
CLINICAL MICROBIOLOGY AND INFECTION
(2017)
Article
Immunology
Chris D. Crowder, Heather E. Carolan, Megan A. Rounds, Vaclav Honig, Benedikt Mothes, Heike Haag, Oliver Nolte, Ben J. Luft, Libor Grubhoffer, David J. Ecker, Steven E. Schutzer, Mark W. Eshoo
EMERGING INFECTIOUS DISEASES
(2014)