期刊
JOURNAL OF CLINICAL MICROBIOLOGY
卷 46, 期 11, 页码 3653-3659出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.01188-08
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- French Armed Forces Medical Service
- French Delegation Generale pour l'Armement [d'objectif 08co402]
The development and validation of a one-step, single-tube, real-time accelerated reverse-transcription loop-mediated isothermal amplification (RT-LAMP) for the detection of the L RNA segment of Rift Valley fever virus (RVFV) are described. The assay was performed at a constant temperature (63 degrees C), with a real-time follow-up using a LightCycler and a double-stranded-DNA-intercalating fluorochrome. The assay is highly sensitive and comparable to real-time RT-PCR, with a detection limit of similar to 10 RNA copies per assay. However, the RT-LAMP assay is much faster than traditional RT-PCR and generates results in < 30 min for most diluted samples. The specificity of the primers was established using other, related arboviruses as well as virus-containing and virus-free sera. The RT-LAMP assay reported here is thus a valuable tool for the rapid detection of RVFV in field diagnostic laboratories.
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