Article
Microbiology
Xi Yang, Qian Liu, Hui Sun, Yanwen Xiong, Andreas Matussek, Xiangning Bai
Summary: This study identified Stx2l-STEC strains from raw mutton and beef in China, showing that these strains belonged to the same O8 serogroup with diverse host range and geographical distribution. Genomic analysis revealed genetic relatedness between human-derived and food-derived strains, highlighting the need for further investigation into the epidemiological traits and pathogenic potential of Stx2l-STEC strains.
Article
Microbiology
Alexander Gill, Forest Dussault, Tanis McMahon, Nicholas Petronella, Xiong Wang, Elizabeth Cebelinski, Flemming Scheutz, Kelly Weedmark, Burton Blais, Catherine Carrillo
Summary: This study identified seven STEC isolates with atypical stx2 sequences that do not fit current Shiga toxin taxonomy. The presence of these new Stx subtypes in clinical isolates suggests their potential as human pathogens.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Microbiology
Xiangning Bai, Flemming Scheutz, Henrik Mellstrom Dahlgren, Ingela Hedenstrom, Cecilia Jernberg
Summary: A novel Stx2m subtype was identified in clinical E. coli strains isolated from Sweden and Denmark, showing functional cytotoxicity and macrolide resistance gene mdf (A). This finding expands the pool of Stx2 subtypes and underscores the clinical significance of emerging STEC variants. This study suggests including Stx2m in epidemiological surveillance and clinical diagnosis of STEC infections.
Article
Microbiology
Xi Yang, Qian Liu, Xiangning Bai, Bin Hu, Deshui Jiang, Hongbo Jiao, Liangmei Lu, Ruyue Fan, Peibin Hou, Andreas Matussek, Yanwen Xiong
Summary: Stx2k-STEC strains have been circulating in different sources in China. We found a high prevalence of Stx2k-STEC in goat herds. More than half of the strains were of the hybrid STEC/ETEC pathotype. Stx2k-STEC strains of specific serotypes have been widespread in goat herds for several years. Stx2k-converting prophages show a high level of similarity across geographical regions and time. The high prevalence of Stx2k-STEC in goats suggests a risk of cross-species spread and potential pathogenicity to humans. Further monitoring of human Stx2k-STEC infections is warranted.
MICROBIOLOGY SPECTRUM
(2022)
Article
Food Science & Technology
Rebecca A. A. Bova, Andrew C. C. Lamont, Theodore J. J. Picou, Vincent B. B. Ho, Kristin H. H. Gilchrist, Angela R. R. Melton-Celsa
Summary: Shiga toxins can cross the intestinal barrier and target kidney endothelial cells, and Stx2a translocates more than Stx1a in both single-layer and three-layer cell models. Infection with different strains of STEC affected barrier function differently, with O157:H7 reducing function and O26:H11 allowing translocation without reducing function.
Article
Food Science & Technology
Anja Mueller, Diana Seinige, Nils T. Grabowski, Birte Ahlfeld, Min Yue, Corinna Kehrenberg
Summary: Insects as novel foods are gaining popularity in Europe. While the presence of Shiga toxin-producing E. coli (STEC) in edible insects poses a potential health hazard, the majority of isolates belong to phylogenetic group A, mainly commensal E. coli. Moreover, the low resistance rate among the isolates indicates a low risk for the transmission of antimicrobial-resistant E. coli to consumers.
Article
Immunology
Mariana Izquierdo, Joaquin Lopez, Pablo Gallardo, Roberto M. Vidal, Juan C. Ossa, Mauricio J. Farfan
Summary: This study evaluated the role of Citrobacter werkmanii and Escherichia albertii in the virulence of Shiga toxin-producing and enteroaggregative Escherichia coli pathotypes, revealing their effects on gene expression and toxin secretion.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Noble Selasi Gati, Imke Johanna Temme, Barbara Middendorf-Bauchart, Alexander Kehl, Ulrich Dobrindt, Alexander Mellmann
Summary: Hybrid Shiga toxin-producing Escherichia coli strains are positioned between STEC and UPEC, causing both diarrhea and urinary tract infections, with potential to adapt to both intestinal and extraintestinal environments.
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Article
Food Science & Technology
Agnieszka Necel, Sylwia Bloch, Bozena Nejman-Falenczyk, Aleksandra Dydecka, Gracja Topka-Bielecka, Alicja Wegrzyn, Grzegorz Wegrzyn
Summary: This study proposes a validation system for selecting bacteriophages against STEC contamination, and conducts model experiments to explore their potential applications. The results show that bacteriophage vB_Eco4M-7 has a certain bactericidal effect on STEC, but caution should be taken due to the appearance of phage-resistant bacteria.
Article
Microbiology
Anouschka Den Ouden, David R. Greig, Ella V. Rodwell, Francesco Tripodo, Israel Olonade, Craig Swift, Claire Jenkins
Summary: This study analyzed clinical outcomes and genome-sequencing data of STEC encoding-stx2f infections in England to assess the risk to public health. The results showed that STEC harboring stx2f can cause severe clinical outcomes, including STEC-HUS. Therefore, it is recommended to strengthen the collection and sharing of microbiological and epidemiological data and further study the animal and environmental reservoirs and transmission routes.
JOURNAL OF MEDICAL MICROBIOLOGY
(2023)
Article
Infectious Diseases
Nicola Mangieri, Roberto Foschino, Claudia Picozzi
Summary: The study found that using bacteriophages can effectively reduce the number of E. coli bacteria producing biofilms, thus improving food safety. Preventing biofilm formation is more effective than trying to remove pre-formed biofilms.
Article
Microbiology
Heather M. M. Blankenship, Stephen E. E. Dietrich, Elizabeth Burgess, Jason Wholehan, Marty Soehnlen, Shannon D. D. Manning
Summary: This study conducted a retrospective analysis of 510 clinical STEC isolates using core genome SNP analysis and found that PFGE and MLST could not fully reveal the genetic diversity and relatedness. SNP analysis was able to cluster isolates based on similarity in PFGE and MLST and uncover their phylogenetic relationships. The use of high-quality SNP analysis enabled a more rapid identification of related strains during outbreak investigations. This study demonstrates how public health laboratories can utilize whole-genome sequencing and phylogenetics to identify related strains and uncover important genetic attributes that can guide treatment practices.
Review
Immunology
Su-bin Hwang, Ramachandran Chelliah, Ji Eun Kang, Momna Rubab, Eric Banan-MwineDaliri, Fazle Elahi, Deog-Hwan Oh
Summary: STEC is a global foodborne bacterial pathogen that can cause severe diarrhea and other critical illnesses, mainly through inflammation caused by Shiga toxins. There is currently no widely accepted treatment for STEC infections, and further research is needed.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Food Science & Technology
Lucrezia Rocchetti, Beatrice Munari, Elisa Varrone, Elisa Porcellini, Dorothea Orth-Hoeller, Reinhard Wuerzner, Domenica Carnicelli, Maurizio Brigotti
Summary: The pathogenesis of eHUS is influenced by pathogenic E. coli producing Stx and host factors. Stx2a is considered the main pathogenic factor in eHUS development, with its functional properties changing based on modifications of toxin structure.
Letter
Immunology
Guillaume Geslain, Aurelie Cointe, Philippe Bidet, Celine Courroux, Soumeth Abasse, Patricia Mariani, Stephane Bonacorsi
Summary: We describe fatal meningitis in 2 neonates in France caused by Shiga toxin 1-producing Escherichia coli. Both strains possessed the virulence factors capsular K1 antigen and salmochelin, potentially suggesting a novel hybrid pathotype. Clinicians should be vigilant about emerging pathotypes and develop therapeutic strategies for neonatal E. coli infections.
EMERGING INFECTIOUS DISEASES
(2023)
Article
Immunology
Anne Kvistholm Jensen, Eva Moller Nielsen, Jonas Torgny Bjorkman, Tenna Jensen, Luise Muller, Soren Persson, Gitte Bjerager, Annette Perge, Tyra Grove Krause, Kristoffer Kiil, Gitte Sorensen, Jens Kirk Andersen, Kare Molbak, Steen Ethelberg
CLINICAL INFECTIOUS DISEASES
(2016)
Article
Biotechnology & Applied Microbiology
Paul I. Costea, Georg Zeller, Shinichi Sunagawa, Eric Pelletier, Adriana Alberti, Florence Levenez, Melanie Tramontano, Marja Driessen, Rajna Hercog, Ferris-Elias Jung, Jens Roat Kultima, Matthew R. Hayward, Luis Pedro Coelho, Emma Allen-Vercoe, Laurie Bertrand, Michael Blaut, Jillian R. M. Brown, Thomas Carton, Stephanie Cools-Portier, Michelle Daigneault, Muriel Derrien, Anne Druesne, Willem M. de Vos, B. Brett Finlay, Harry J. Flint, Francisco Guarner, Masahira Hattori, Hans Heilig, Ruth Ann Luna, Johan van Hylckama Vlieg, Jana Junick, Ingeborg Klymiuk, Philippe Langella, Emmanuelle Le Chatelier, Volker Mai, Chaysavanh Manichanh, Jennifer C. Martin, Clementine Mery, Hidetoshi Morita, Paul W. O'Toole, Celine Orvain, Kiran Raosaheb Patil, John Penders, Soren Persson, Nicolas Pons, Milena Popova, Anne Salonen, Delphine Saulnier, Karen P. Scott, Bhagirath Singh, Kathleen Slezak, Patrick Veiga, James Versalovic, Liping Zhao, Erwin G. Zoetendal, S. Dusko Ehrlich, Joel Dore, Peer Bork
NATURE BIOTECHNOLOGY
(2017)
Article
Infectious Diseases
Alexandre de Almeida Monteiro, Renata Neto Pires, Soren Persson, Edison Moraes Rodrigues Filho, Alessandro Comaru Pasqualotto
BRAZILIAN JOURNAL OF INFECTIOUS DISEASES
(2014)
Article
Infectious Diseases
Soren Persson, Richard F. de Boer, Anna M. D. Kooistra-Smid, Katharina E. P. Olsen
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2011)
Editorial Material
Infectious Diseases
H. Nielsen, S. Persson, K. E. P. Olsen, T. Ejlertsen, B. Kristensen, H. C. Schonheyder
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2010)
Article
Infectious Diseases
L. M. Soes, I. Brock, S. Persson, J. Simonsen, K. E. Pribil Olsen, M. Kemp
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2012)
Article
Microbiology
Soren Persson, Joan N. Jensen, Katharina E. P. Olsen
JOURNAL OF CLINICAL MICROBIOLOGY
(2011)
Article
Microbiology
Soren Persson, Suzan Al-Shuweli, Seval Yapici, Joan N. Jensen, Katharina E. P. Olsen
JOURNAL OF CLINICAL MICROBIOLOGY
(2015)
Article
Immunology
Nadia Boisen, Flemming Scheutz, David A. Rasko, Julia C. Redman, Soren Persson, Jakub Simon, Karen L. Kotloff, Myron M. Levine, Samba Sow, Boubou Tamboura, Aliou Toure, Dramane Malle, Sandra Panchalingam, Karen A. Krogfelt, James P. Nataro
JOURNAL OF INFECTIOUS DISEASES
(2012)
Article
Microbiology
Olivia Graaf Bjoersdorff, Sanna Lindberg, Kristoffer Kiil, Soren Persson, Luca Guardabassi, Peter Damborg
Summary: There is a concern about animals as reservoirs of Clostridioides difficile. In this study, prevalence, antimicrobial resistance, and zoonotic potential of C. difficile in dogs were investigated. It was found that a small proportion of faecal deposits contained toxigenic C. difficile such as ST2 (RT014/020), which is a major cause of community-acquired infections, suggesting the exchange of pathogenic strains between dogs and humans.
Article
Infectious Diseases
Soren Persson, Hans Linde Nielsen, John Eugenio Coia, Jorgen Engberg, Bente Scharvik Olesen, Anne Line Engsbro, Andreas Munk Petersen, Hanne Marie Holt, Lars Lemming, Ea Sofie Marmolin, Turid Snekloth Sondergaard, Leif Percival Andersen, Mie Birgitte Frid Jensen, Camilla Wiuff, Gitte Sorensen, Sofie Holtsmark Nielsen, Eva Moller Nielsen
Summary: This study established a new sentinel surveillance scheme to monitor C. difficile infections in Denmark. The results showed geographical differences and temporal changes in the distribution of different types of C. difficile infections.
Article
Microbiology
C. B. Harder, S. Persson, J. Christensen, A. Ljubic, E. M. Nielsen, J. Hoorfar
Summary: The study found that Salmonella and Campylobacter DNA can be easily detected in human, pig, and chicken stool samples stored at different temperatures and with different preservation methods, suggesting that infections by these bacteria can be diagnosed simply by taking a stool sample in any suitable sealed container.
Article
Microbiology
Sandra Christine Andersen, Kristoffer Kiil, Christoffer Bugge Harder, Mathilde Hasseldam Josefsen, Soren Persson, Eva Moller Nielsen, Jeffrey Hoorfar
Article
Infectious Diseases
L. Soes, K. Molbak, S. Strobaek, K. Truberg Jensen, M. Torpdahl, S. Persson, M. Kemp, K. E. Olsen