Article
Microbiology
Alejandro Dashti, Henar Alonso, Cristina Escolar-Minana, Pamela C. Koster, Begona Bailo, David Carmena, David Gonzalez-Barrio
Summary: Thorough independent assessment of the diagnostic performance of novel diagnostic assays is essential. In this study, the VIASURE Cryptosporidium, Giardia, & E. histolytica real-time PCR assay was evaluated for its performance in detecting and identifying enteric protozoan parasites. The assay demonstrated good performance for routine testing in clinical microbiological laboratories.
MICROBIOLOGY SPECTRUM
(2022)
Review
Environmental Sciences
Jun-Yang Ma, Man-Yao Li, Ze-Zheng Qi, Ming Fu, Tian-Feng Sun, Hany M. Elsheikha, Wei Cong
Summary: The review highlights a higher prevalence of waterborne protozoan outbreaks in developed countries, particularly associated with Cryptosporidium and Giardia. Variations in the number of outbreaks between geographic regions are likely attributed to differences in diagnostic capabilities and surveillance programs for monitoring water contamination.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Parasitology
Caixia Dong, Ziye Liu, Xiaolin Li
Summary: Our Giardia chemiluminescence assay (GCA) detected Giardia antigens in a dose-dependent manner with a detection limit of 0.46 ng/mL and a signal-to-baseline ratio of 475. In a study using 30 clinic collected canine stool samples, the GCA showed a sensitivity of 93.8% and a specificity of 92.9% compared to the standard Giardia II ELISA. Additionally, the GCA displayed higher signal-to-baseline ratios and equal or higher antigen titers compared to a colorimetric ELISA in a study of 16 Giardia positive samples.
VETERINARY PARASITOLOGY
(2023)
Article
Infectious Diseases
A. S. Muadica, S. Balasegaram, K. Beebeejaun, P. C. Koster, B. Bailo, M. Hernandez-de-Mingo, A. Dashti, E. Dacal, J. M. Saugar, I Fuentes, D. Carmena
Summary: The study found a high prevalence of intestinal parasites in children regardless of symptoms. Drinking well or river water, contact with domestic animals, and lack of latrines were contributing factors to human infections.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Veterinary Sciences
Miranda H. J. Huang, Steve Demarais, W. Cooper Brookshire, Bronson K. Strickland
Summary: Concentrating animals artificially may increase the risk of disease spread, and supplemental feeding of deer can increase the environmental load of gastrointestinal parasites (GIPs). The type of feeder used also affects GIP load.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Parasitology
Christian M. Leutenegger, Cecilia E. Lozoya, Jeffrey Tereski, Jan Andrews, Kelly D. Mitchell, Cathy Meeks, Jennifer L. Willcox, Gregory Freeman, Holly L. Richmond, Christian Savard, Michelle D. Evason
Summary: This study compared the performance of real-time PCR (qPCR) and zinc sulfate centrifugal fecal flotation microscopy (ZCF) for fecal parasite screening. The results showed that qPCR had a significantly higher detection frequency than ZCF and could detect parasite markers that were undetected by ZCF.
PARASITES & VECTORS
(2023)
Article
Multidisciplinary Sciences
Naimat Ullah Khan, Tahir Usman, Mian Saeed Sarwar, Hazrat Ali, Ali Gohar, Muhammad Asif, Fazli Rabbani, Rifat Ullah Khan, Nighat Sultana, Nazir Ahmad Khan, Muhammad Mobashar, Assar Ali Shah, Metha Wanapat
Summary: This study investigated the prevalence and risk factors of Cryptosporidium infection in diarrheic sheep in Pakistan, comparing the sensitivity of two diagnostic techniques. The research concluded that molecular detection through PCR is more efficient, specific, and sensitive than simple microscopy for detecting Cryptosporidium infection.
Article
Multidisciplinary Sciences
Naimat Ullah Khan, Tahir Usman, Mian Saeed Sarwar, Hazrat Ali, Ali Gohar, Muhammad Asif, Fazli Rabbani, Rifat Ullah Khan, Nighat Sultana, Nazir Ahmad Khan, Muhammad Mobashar, Assar Ali Shah, Metha Wanapat
Summary: The study investigated the prevalence and risk factors of Cryptosporidium infection in diarrheic sheep in Pakistan using simple microscopy and PCR techniques. Results showed age and sex were important factors affecting prevalence, with molecular detection being more efficient and sensitive compared to simple microscopy.
Review
Engineering, Environmental
Christina M. Morrison, Samantha Hogard, Robert Pearce, Daniel Gerrity, Eric C. Wert, Urs von Gunten
Summary: This study analyzed ozone disinfection data for viruses, Giardia cysts, and C. parvum oocysts, and found that ozone's inactivation efficiency decreases in the order of viruses >> Giardia cysts > C. parvum oocysts. The USEPA Ct models accurately predicted inactivation of C. parvum oocysts and viruses, but overestimated inactivation of Giardia cysts. Common surrogate organisms showed different inactivation kinetics compared to mammalian viruses and C. parvum oocysts.
Review
Microbiology
Blaine A. A. Mathison, Richard S. S. Bradbury, Bobbi S. S. Pritt
Summary: This minireview provides an update on the recent advancements and updates in the field of human parasitology from June 2020 to June 2022. It also includes a list of previously reported nomenclatural changes that have not been widely adopted by the medical community.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Review
Environmental Sciences
Pilar Suarez, Jose Luis Alonso, Gloria Gomez, Gladys Vidal
Summary: This study evaluated the removal or inactivation efficiency of different treatment technologies for Cryptosporidium sp. and Giardia sp. It was found that aerobic technologies had the best removal efficiency for Cryptosporidium sp., while technologies combining filtration with UV or chemical disinfection agents showed the greatest removal efficiency for Giardia sp. However, detecting and quantifying the infectious forms of these parasites in effluents remains a major concern.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Review
Parasitology
Una M. Ryan, Yaoyu Feng, Ronald Fayer, Lihua Xiao
Summary: Protozoan parasites Cryptosporidium and Giardia are significant causes of diarrhoea worldwide, with numerous named species and genotypes recognized. Zoonotic transmission plays an important role in the epidemiology, and the development of sequencing tools provides evidence for this. Comparative whole genome sequencing offers key information on genetic mechanisms and enables future One Health management.
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2021)
Article
Environmental Sciences
Fernanda Queiroz Valdez, Luan de Souza Leite, Jose Antonio Zanetoni Filho, Mariana Daniel Tango, Luiz Antonio Daniel
Summary: This study investigated the occurrence and removal of Giardia spp. cysts and Cryptosporidium spp. oocysts by three different UASB reactors in Brazil, finding that hydraulic retention time may be the key parameter for Giardia spp. removal. No parameter analysed showed a common correlation with the (oo)cyst concentration in the three UASB reactors.
ENVIRONMENTAL TECHNOLOGY
(2022)
Article
Immunology
Chi Sun Yun, Bo-Youn Moon, Kichan Lee, Su Min Kang, Bok-Kyung Ku, Mi-Hye Hwang
Summary: This study aimed to detect and identify subspecies of gastrointestinal protozoa parasites in cats from South Korea. The results showed a high rate of infection with cat-specific Cryptosporidium and Cystoisospora, which are associated with living conditions and diarrhea symptoms in South Korean cats. Zoonotic and other animal-specific subtype of protozoan parasites have also been detected in cat feces.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Laura Lux, Rainer G. Ulrich, Sergio Santos-Silva, Joao Queiros, Christian Imholt, Christian Klotz, Joana Pauperio, Ricardo Pita, Helia Vale-Goncalves, Paulo Celio Alves, Joao R. Mesquita
Summary: This study is the first investigation of Cryptosporidium and Giardia parasites in wild rodents and shrews in Portugal. The results show that Cryptosporidium was rarely detected, while Giardia was frequently detected, with the highest infection rates found in southwestern water voles and Lusitanian pine voles. Genetic characterization revealed rodent-adapted Giardia microti and potentially zoonotic Cryptosporidium muris as the only circulating species. This study suggests that wild rodents and shrews have a limited role as natural sources of human infections in Portugal.
Article
Immunology
Poonum S. Korpe, Carol Gilchrist, Cecelia Burkey, Mami Taniuchi, Emtiaz Ahmed, Vikram Madan, Rachel Castillo, Shahnawaz Ahmed, Tuhinur Arju, Masud Alam, Mamun Kabir, Tahmeed Ahmed, William A. Petri, Rashidul Haque, A. S. G. Faruque, Priya Duggal
CLINICAL INFECTIOUS DISEASES
(2019)
Editorial Material
Immunology
Kevin L. Steiner, Mamun Kabir, Jeffrey W. Priest, Biplob Hossain, Carol A. Gilchrist, Heather Cook, Jennie Z. Ma, Poonum S. Korpe, Tahmeed Ahmed, A. S. G. Faruque, Rashidul Haque, William A. Petri
CLINICAL INFECTIOUS DISEASES
(2020)
Article
Multidisciplinary Sciences
Sarah K. G. Jensen, Fahmida Tofail, Rashidul Hague, William A. Petri, Charles A. Nelson
Article
Multidisciplinary Sciences
Alyse L. Frisbee, Mahmoud M. Saleh, Mary K. Young, Jhansi L. Leslie, Morgan E. Simpson, Mayuresh M. Abhyankar, Carrie A. Cowardin, Jennie Z. Ma, Patcharin Pramoonjago, Stephen D. Turner, Alice P. Liou, Erica L. Buonomo, William A. Petri
NATURE COMMUNICATIONS
(2019)
Article
Immunology
Jeffrey R. Donowitz, Hardik I. Parikh, Mami Taniuchi, Carol A. Gilchrist, Rashidul Haque, Beth D. Kirkpatrick, Masud Alam, Shahria Hafiz Kakon, Bushra Zarin Islam, Sajia Afreen, Mamun Kabir, Uma Nayak, E. Ross Colgate, Marya P. Carmolli, William A. Petri
OPEN FORUM INFECTIOUS DISEASES
(2019)
Review
Immunology
Mahmoud M. Saleh, William A. Petri
INFECTION AND IMMUNITY
(2020)
Article
Nutrition & Dietetics
Benjamin J. J. McCormick, Stephanie A. Richard, Laura E. Caulfield, Laura L. Pendergast, Jessica C. Seidman, Beena Koshy, Reeba Roshan, Rita Shrestha, Erling Svensen, Ladislaus Blacy, Zeba Rasmussen, Angelina Maphula, Rebecca Scharf, Baitun Nahar, Sayma Haque, Muneera Rasheed, Reinaldo Oria, Elizabeth T. Rogawski, Laura E. Murray-Kolb, Angel Mendez Acosta, Rosa Rios de Burga, Cesar Banda Chavez, Julian Torres Flores, Maribel Paredes Olotegui, Silvia Rengifo Pinedo, Mery Siguas Salas, Dixner Rengifo Trigoso, Angel Orbe Vasquez, Imran Ahmed, Didar Alam, Asad Ali, Zulfiqar A. Bhutta, Shahida Qureshi, Sajid Soofi, Ali Turab, Anita K. M. Zaidi, Ladaporn Bodhidatta, Carl J. Mason, Sudhir Babji, Anuradha Bose, Ajila T. George, Dinesh Hariraju, M. Steffi Jennifer, Sushil John, Shiny Kaki, Gagandeep Kang, Priyadarshani Karunakaran, Robin P. Lazarus, Jayaprakash Muliyil, Mohan Venkata Raghava, Sophy Raju, Anup Ramachandran, Rakhi Ramadas, Karthikeyan Ramanujam, Srujan L. Sharma, Shanmuga E. Sundaram, Rahul J. Thomas, William K. Pan, Ramya Ambikapathi, J. Daniel Carreon, Vivek Charu, Viyada Doan, Jhanelle Graham, Christel Hoest, Stacey Knobler, Dennis R. Lang, Monica McGrath, Mark A. Miller, Archana Mohale, Gaurvika Nayyar, Stephanie Psaki, Zeba Rasmussen, Vivian Wang, Rebecca Blank, Michael Gottlieb, Karen H. Tountas, Caroline Amour, Eliwaza Bayyo, Estomih R. Mduma, Regisiana Mvungi, Rose-Mary Nshama, John Pascal, Buliga Mujaga Swema, Ladislaus Yarrot, Tahmeed Ahmed, A. M. Shamsir Ahmed, Rashidul Haque, Iqbal Hossain, Munirul Islam, Mustafa Mahfuz, Dinesh Mondal, Fahmida Tofail, Ram Krishna Chandyo, Prakash Sunder Shrestha, Manjeswori Ulak, Aubrey Bauck, Robert E. Black, William Checkley, Margaret N. Kosek, Gwenyth Lee, Kerry Schulze, Pablo Penataro Yori, A. Catharine Ross, Barbara Schaefer, Suzanne Simons, Samuel P. Scott, Claudia B. Abreu, Hilda Costa, Alessandra Di Moura, Jose Quirino Filho, Alexandre Havt, Alvaro M. Leite, Aldo A. M. Lima, Noelia L. Lima, Ila F. Lima, Bruna L. L. Maciel, Pedro H. Q. S. Medeiros, Milena Moraes, Francisco S. Mota, Josiane Quetz, Alberto M. Soares, Rosa M. S. Mota, Crystal L. Patil, Pascal Bessong, Cloupas Mahopo, Emanuel Nyathi, Amidou Samie, Leah Barrett, Rebecca Dillingham, Jean Gratz, Richard L. Guerrant, Eric Houpt, William A. Petri, James Platts-Mills, Binob Shrestha, Sanjaya Kumar Shrestha, Tor Strand
JOURNAL OF NUTRITION
(2019)
Article
Multidisciplinary Sciences
Md. Abu Musa, Mamun Kabir, Md. Iqbal Hossain, Emtiaz Ahmed, Abdullah Siddique, Humaira Rashid, Mustafa Mahfuz, Dinesh Mondal, Tahmeed Ahmed, William A. Petri, Rashidul Haque
Article
Nutrition & Dietetics
Mustafa Mahfuz, Mohammed Ashraful Alam, Subhasish Das, Shah Mohammad Fahim, Md Shabab Hossain, William A. Petri, Per Ashorn, Ulla Ashorn, Tahmeed Ahmed
JOURNAL OF NUTRITION
(2020)
Article
Microbiology
Genevieve L. Wojcik, Poonum Korpe, Chelsea Marie, Alexander J. Mentzer, Tommy Carstensen, Josyf Mychaleckyj, Beth D. Kirkpatrick, Stephen S. Rich, Patrick Concannon, A. S. G. Faruque, Rashidul Haque, William A. Petri, Priya Duggal
Article
Gastroenterology & Hepatology
Alexandra Donlan, William A. Petri
CLINICS IN COLON AND RECTAL SURGERY
(2020)
Article
Neurosciences
Ted Turesky, Wanze Xie, Swapna Kumar, Danielle D. Sliva, Borjan Gagoski, Jennifer Vaughn, Lilla Zollei, Rashidul Haque, Shahria Hafiz Kakon, Nazrul Islam, William A. Petri, Charles A. Nelson, Nadine Gaab
Article
Psychology, Developmental
Katherine L. Perdue, Sarah K. G. Jensen, Swapna Kumar, John E. Richards, Shahria Hafiz Kakon, Rashidul Haque, William A. Petri, Sarah Lloyd-Fox, Clare Elwell, Charles A. Nelson
DEVELOPMENTAL SCIENCE
(2019)
Article
Psychology, Developmental
Ted K. Turesky, Sarah K. G. Jensen, Xi Yu, Swapna Kumar, Yingying Wang, Danielle D. Silva, Borjan Gagoski, Joseph Sanfilippo, Lilla Zollei, Emma Boyd, Rashidul Hague, Shahria Hafiz Kakon, Nazrul Islam, William A. Petri, Charles A. Nelson, Nadine Gaab
DEVELOPMENTAL SCIENCE
(2019)
Article
Gastroenterology & Hepatology
Koji Watanabe, William A. Petri
