Article
Immunology
Catherine A. Hogan, ChunHong Huang, Malaya K. Sahoo, Hannah Wang, Becky Jiang, Mamdouh Sibai, Marisa Holubar, Roshni Mathew, James Zehnder, Benjamin A. Pinsky
Summary: A method that detects minus-strand RNA as a surrogate for actively replicating SARS-CoV-2 was developed and found in 41 patients up to 30 days after symptom onset. This assay could potentially impact clinical decision-making regarding patient infectiousness.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Virology
Xiuhong Wu, Yuanjia Liu, Liguo Gao, Zhuanqiang Yan, Qiqi Zhao, Feng Chen, Qingmei Xie, Xinheng Zhang
Summary: The study developed an isothermal amplification technology to achieve molecular diagnosis of PEDV in clinical samples from China by using RT-RAA combined with portable instruments. The method showed strong specificity and high detection sensitivity, making it more suitable for real-time prevention and control of PEDV.
Article
Infectious Diseases
Gaurav Rawal, Wannarat Yim-Im, Fabian Chamba, Chad Smith, Jeff Okones, Charles Francisco, Jianqiang Zhang
Summary: PRRSGard PCR, a newly developed specific real-time PCR, is able to detect PRRSGard vaccine-like virus without cross-reacting with other PRRSV vaccine viruses or field isolates. This PCR method shows high sensitivity and accuracy, and performs well in detecting PRRSGard vaccine virus.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Virology
Long Zhao, Guoqing Shao, Cheng Tang, Hua Yue
Summary: This study confirms BToV in yaks for the first time and reports a recombination event in the S gene, with the virus showing diverse genotypes and high specificity and reproducibility.
ARCHIVES OF VIROLOGY
(2021)
Article
Veterinary Sciences
Lei Ma, Kaiqi Lian, Mengjie Zhu, Yajie Tang, Mingliang Zhang
Summary: A novel RT-RPA-LFD assay was established for the visual detection of PEDV with high sensitivity and specificity. The assay showed potential for use in resource-limited areas and countries.
BMC VETERINARY RESEARCH
(2022)
Article
Chemistry, Analytical
Wei Feng, Hanyong Peng, Jingyang Xu, Yanming Liu, Kanti Pabbaraju, Graham Tipples, Michael A. Joyce, Holly A. Saffran, D. Lorne Tyrrell, Shawn Babiuk, Hongquan Zhang, X. Chris Le
Summary: The study successfully integrated RT-RPA and CRISPR-Cas12a into a single tube for viral sequence detection, overcoming issues like slow initiation of RPA and non-specific amplification, significantly improving detection speed and accuracy.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Catherine T. Chia, Andrew T. Bender, Lorraine Lillis, Benjamin P. Sullivan, Coleman D. Martin, Wynn Burke, Charles Landis, David S. Boyle, Jonathan D. Posner
Summary: This study reports a rapid, highly accurate, and minimally instrumented assay for HCV RNA detection using RT-RPA. The assay can detect all HCV genotypes with a limit of detection of 25 copies per reaction for genotype 1. Clinical experiments showed that the RT-RPA assay has a sensitivity and specificity of 100%. The accuracy, short runtime, and low heating demands of RT-RPA make it potentially valuable for point-of-care HCV testing.
Article
Chemistry, Multidisciplinary
Huina Wu, Mingyuan Zou, Xiaobo Fan, Feiya Su, Feng Xiao, Meiling Zhou, Yan Sun, Fengfeng Zhao, Guoqiu Wu
Summary: A catalytic hairpin assembly (CHA) combined with lateral flow immunoassay (CHA-LFIA) method was proposed for rapid and precise detection of influenza viruses and RSV, demonstrating high sensitivity and specificity.
Article
Public, Environmental & Occupational Health
Christine F. Markwalter, Billy Ngasala, Tonelia Mowatt, Christopher Basham, Zackary Park, Mwajabu Loya, Meredith Muller, Christopher Plowe, Myaing Nyunt, Jessica T. Lin
Summary: Ultrasensitive PCR has shown a higher burden of asymptomatic malaria infections compared to RDTs or standard PCR in low-transmission endemic areas, with potential implications for the infectious reservoir. A study in Tanzania found good correlation between DNA-based qPCR and RNA-based usPCR in detecting Plasmodium falciparum parasite densities, indicating an incremental increase in low-density infections. The extent to which these low-density infections contribute to the infectious reservoir in different malaria transmission settings remains unclear.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2021)
Article
Biology
Francesca Marino-Merlo, Valeria Stefanizzi, Agnese Ragno, Lucia Piredda, Sandro Grelli, Beatrice Macchi, Antonio Mastino
Summary: The study developed a real-time reverse transcriptase quantitative PCR (RT-qPCR) assay to assess very low levels of HIV-1 RT activity based on previous laboratory experience. A synthetic RNA was used as a template for reverse transcription into cDNA by HIV-1 RT, and different variables were tested to optimize the assay. The optimized gD-RNA-synt-based RT assay demonstrated high sensitivity, allowing for the detection of specific cDNA reverse transcription even in the presence of 1 x 10(-9) U HIV RT. This constructed RT-qPCR assay shows promise in accurately assessing very low HIV-1 RT activity.
Article
Chemistry, Analytical
Ian T. Hull, Enos C. Kline, Gaurav K. Gulati, Jack Henry Kotnik, Nuttada Panpradist, Kamal G. Shah, Qin Wang, Lisa Frenkel, James Lai, Joanne Stekler, Barry R. Lutz
Summary: The study presents a novel quantitative detection method for HIV DNA or RNA using a combination of recombinase polymerase amplification and lateral flow detection technologies, allowing for rapid and accurate testing in low-resource healthcare settings, with the potential to improve the quality of HIV treatment.
ANALYTICAL CHEMISTRY
(2022)
Article
Agronomy
Hyo-Jeong Lee, Sena Choi, In-Sook Cho, Ju-Yeon Yoon, Rae-Dong Jeong
Summary: In this study, a sensitive and accurate quantification method for PlAMV infection in lily samples using a reverse transcription droplet digital polymerase chain reaction (RT-ddPCR) assay was established. The RT-ddPCR assay showed high specificity and sensitivity for PlAMV detection and quantification, outperforming the traditional RT-qPCR method. Field validation tests further confirmed the superior sensitivity of RT-ddPCR. This method can be used for PlAMV infection diagnosis, especially in plant quarantine inspection and PlAMV-free certification programs.
Article
Microbiology
Bryanna Lexus Freitas, Lynn Leach, Vishnu Chaturvedi, Sudha Chaturvedi
Summary: A new detection method has been developed to rapidly detect live multidrug-resistant yeast Candida auris in healthcare environments. This method is highly sensitive and specific, and it correlates with the results from traditional culture methods.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Virology
Alexandra L. Chang-Graham, Malaya K. Sahoo, ChunHong Huang, Daniel Solis, Mamdouh Sibai, Gianna August, Lira Calayag, Obadia M. Kenji, Run-Zhang Shi, Heba H. Mostafa, Guang-Sheng Lei, Ryan F. Relich, Benjamin A. Pinsky
Summary: The qualitative agreement of the DiaSorin LIAISON SARS-CoV-2 nucleocapsid antigen chemiluminescent immunoassay (CLIA) with minus-strand RNA was evaluated using upper respiratory specimens. The results showed a high level of agreement and suggested that this method can provide valuable information for infection control and patient management.
JOURNAL OF CLINICAL VIROLOGY
(2023)
Article
Multidisciplinary Sciences
Xi-Wen Jiang, Tao-Sheng Huang, Long Xie, Si-Ze Chen, Shi-Dong Wang, Zhi-Wen Huang, Xin-Yu Li, Wei-Ping Ling
Summary: Acute respiratory infections are common in vulnerable populations and can be caused by various microorganisms. Early detection through rapid pathogen screening is crucial. This study developed a multiplex PCR assay that can simultaneously detect nine respiratory pathogens, offering rapid, sensitive, and contamination-free detection.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Hector R. Rangel, Gonzalo Bello, Julian A. Villalba, Yoneira F. Sulbaran, Domingo Garzaro, Mailis Maes, Carmen L. Loureiro, Jacobus H. de Waard, Flor H. Pujol
AIDS RESEARCH AND HUMAN RETROVIRUSES
(2015)
Article
Public, Environmental & Occupational Health
Gladys Medina, Domingo J. Garzaro, Miguel Barrios, Albert J. Auguste, Scott C. Weaver, Flor H. Pujol
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2015)
Article
Infectious Diseases
Joseph T. Ortega, Alirica I. Suarez, Maria L. Serrano, Jani Baptista, Flor H. Pujol, Hector R. Rangel
AIDS RESEARCH AND THERAPY
(2017)
Article
Multidisciplinary Sciences
Ruth Y. Blanco, Carmen L. Loureiro, Julian A. Villalba, Yoneira F. Sulbaran, Mailis Maes, Jacobus H. de Waard, Hector R. Rangel, Rossana C. Jaspe, Flor H. Pujol
Article
Microbiology
Rossana C. Jaspe, Yoneira F. Sulbaran, Carmen L. Loureiro, Nahir Martinez, Marisol Devesa, Yesseima Rodriguez, Jaime R. Torres, Hector R. Rangel, Flor H. Pujol
JOURNAL OF MEDICAL MICROBIOLOGY
(2014)
Article
Multidisciplinary Sciences
Carmen L. Loureiro, Julio C. Aguilar, Jorge Aguiar, Verena Muzio, Eduardo Penton, Daymir Garcia, Gerardo Guillen, Flor H. Pujol
Article
Virology
Maria Soderlund-Venermo, Anupam Varma, Deyin Guo, Douglas P. Gladue, Emma Poole, Flor H. Pujol, Hanu Pappu, Jesus L. Romalde, Laura Kramer, Mariana Baz, Marietjie Venter, Matthew D. Moore, Michael M. Nevels, Sayeh Ezzikouri, Vikram N. Vakharia, William C. Wilson, Yashpal S. Malik, Zhengli Shi, Ahmed S. Abdel-Moneim
Summary: This article summarizes the presentations given at the 1st international conference of the World Society for Virology (WSV). The conference focused on tackling global viral epidemics and aimed to foster international collaborations. The conference consisted of multiple sessions and attracted 270 scientists from 49 countries.
Article
Medicine, Research & Experimental
Rossanct C. Jaspe, Jose Luis Zambrano, Mariana Hidalgo, Yoneira Sulbaran, Carmen L. Loureiro, Zoila C. Moros, Domingo J. Garzaro, Ferdinando Liprandi, Hector R. Rangel, Flor H. Pujol
Summary: This study describes a rapid method for identifying cases infected with the Omicron variant and provides more information on the prevalence and rapid detection of this new variant.
INVESTIGACION CLINICA
(2022)
Editorial Material
Microbiology
Isabelle Chemin, Flor Helene Pujol
Article
Virology
Rossana C. Jaspe, Carmen L. Loureiro, Yoneira Sulbaran, Zoila C. Moros, Pierina D'Angelo, Mariana Hidalgo, Lieska Rodriguez, Victor Alarcon, Marwan Aguilar, Doneyla Sanchez, Jesus Ramirez, Domingo J. Garzaro, Jose Luis Zambrano, Ferdinando Liprandi, Hector R. Rangel, Flor H. Pujol
Summary: The aim of this study was to monitor the dissemination of VOIs and VOCs in Venezuela, and it found that Delta VOC became the predominant circulating variant, while Omicron VOC was able to displace Delta variant within a month.
Article
Infectious Diseases
Pierina D'Angelo, Carmen L. L. Loureiro, Rossana C. C. Jaspe, Yoneira F. F. Sulbaran, Lieska Rodriguez, Victor Alarcon, Jose Manuel Garcia, Jose Luis Zambrano, Ferdinando Liprandi, Hector R. Rangel, Flor H. H. Pujol
Summary: The ongoing epidemic of monkeypox virus (MPXV) infection has affected over 50,000 individuals worldwide until the end of August 2022. We present the first case detected in Venezuela, where the patient had traveled from Spain and had contact with friends who tested positive for MPXV. Phylogenetic analysis categorized the isolate within the major circulating clade II of MPXV globally. As of August 2022, no other cases of MPXV have been detected in Venezuela, though undiagnosed cases cannot be ruled out due to the fear of stigmatization.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2023)
Article
Virology
Zoila C. Moros, Jose Luis Zambrano, Yoneira Sulbaran, Carmen L. Loureiro, Ernestina Marulanda, Francis Bracho, Pierina D'Angelo, Lieska Rodriguez, Ferdinando Liprandi, Hector R. Rangel, Rossana C. Jaspe, Flor H. Pujol
Summary: The Omicron Variant of Concern (VOC) has caused a surge in cases worldwide, leading to subsequent waves in many countries. Venezuela has monitored the circulation of the Omicron VOC and its major sub-lineages among community cases and international travelers. Sub-lineages generally circulated earlier in international travelers than in community cases, and the diversity of sub-lineages found in international travelers resembled those found in the USA.
Review
Microbiology
Rayana Maryse Toye, Carmen Luisa Loureiro, Rossana Celeste Jaspe, Fabien Zoulim, Flor Helene Pujol, Isabelle Chemin
Summary: HBV genotypes E to J are understudied and have specific distributions across different regions. Genotype F is the most divergent and has further subgenotypes. There is a lack of data from sub-Saharan Africa and Latin America due to under-representation in clinical and research cohorts.
Article
Medicine, Research & Experimental
Mary L. Puche, Sharon Kay-Valero, Pedro Michelli, Maria D. Oropeza, Carmen L. Loureiro, Marisol Devesa, Lucy Dagher, Flor H. Pujol
INVESTIGACION CLINICA
(2016)
Article
Medicine, Research & Experimental
Maria Zulay Sulbaran, Lerida Montano, Yoneira Sulbaran, Carmen Luisa Loureiro, Carmen Rosa Flores, Yurviris Farias, Antonio Maldonado, Genny Guillen, Hector Rangel, Flor Pujol
INVESTIGACION CLINICA
(2016)
