Article
Engineering, Biomedical
Kevin T. Campbell, Matthew B. Curtis, Joshua M. Massey, Kajetan Wysoczynski, Dustin J. Hadley, Steve C. George, Eduardo A. Silva
Summary: Lymphatic dysfunction is associated with the progression of several vascular disorders, and there are limited strategies currently available to promote new lymphatic vasculature. This study isolated lymphatic endothelial progenitor cells from human umbilical cord blood and found that they have the potential to promote lymphatic sprouts, highlighting the therapeutic potential of these cells. Delivery of lymphatic EPCs using alginate hydrogels also showed promise for possible therapeutic applications.
ACTA BIOMATERIALIA
(2021)
Review
Biotechnology & Applied Microbiology
Hehe He, Mingfei Yang, Siyu Li, Gaoyang Zhang, Zhongyang Ding, Liang Zhang, Guiyang Shi, Youran Li
Summary: This review comprehensively summarizes the mechanisms, identification methods, and applications of transcription factors in various biotechnological fields. It also discusses the current limitations of research and provides prospects for future investigations.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Shuxiang Cai, Yalin Sun, Zhen Wang, Wenguang Yang, Xiangyu Li, Haibo Yu
Summary: E-jet printing is a micro- and nano-manufacturing technique that offers better control and resolution than traditional jet printing methods. It has advantages in terms of material applicability and has been successfully used in various applications. This article provides an overview of E-jet printing technology, including its principle, process, and future development prospects.
NANOTECHNOLOGY REVIEWS
(2021)
Review
Food Science & Technology
Shichen Zhu, Hao Zhu, Siyao Xu, Shuangbao Lv, Shulai Liu, Yuting Ding, Xuxia Zhou
Summary: This review focuses on the stabilization mechanisms of gel-type emulsified muscle products, including factors such as processing conditions, lipids, and emulsifiers. Additionally, some novel systems are introduced, and the promising prospects of emulsion muscle products are elaborated.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2022)
Article
Peripheral Vascular Disease
Katsuki Okuyama, Koji Mizuno, Keisuke Nittami, Hiroaki Sakaue, Takashi Sato
Summary: In this study, it was found that cyclic phosphatidic acid (cPA) can enhance the tube formation of HDLECs through the LPA6/ROCK and LPA1/3 signaling pathways, regulating the lymphatic capillary-like morphogenesis and migration adjacent to type I collagen under physiological conditions.
MICROVASCULAR RESEARCH
(2022)
Article
Rheumatology
Henriette Didriksen, Oyvind Molberg, Havard Fretheim, Einar Gude, Suzana Jordan, Cathrine Brunborg, Vyacheslav Palchevskiy, Torhild Garen, Oyvind Midtvedt, Arne K. Andreassen, Oliver Distler, John Belperio, Anna-Maria Hoffmann-Vold
Summary: This study assessed the role of lymphangiogenic factors in patients with SSc, finding that VEGF-C could be a promising marker for early PAH detection.
ARTHRITIS & RHEUMATOLOGY
(2021)
Article
Energy & Fuels
Xuewen Cao, Hongchao Wang, Kairan Yang, Shichuan Wu, Qian Chen, Jiang Bian
Summary: This review paper summarizes the development status and existing problems of various hydrate-based CO2 sequestration methods and analyzes the mechanisms, influencing factors, and appropriate sequestration locations. It focuses on the CO2 replacement reaction of methane hydrates technology and discusses the feasibility, thermodynamics, kinetics, and methods to improve the replacement efficiency. The paper also discusses the method of direct injection of CO2 into marine sediments to form hydrates for sequestration and generalizes appropriate sequestration locations. Furthermore, it introduces a hydrate-based CO2 sequestration method for reusing depleted deposits.
JOURNAL OF PETROLEUM SCIENCE AND ENGINEERING
(2022)
Review
Chemistry, Physical
Jiahui Zhang, Dongxin Zhao, Kui Lu
Summary: This article investigates the structure and characteristics of self-assembled peptide-based hydrogels and the mechanism of several regular secondary structures in gelation. The factors influencing the formation of peptide hydrogels, especially pH responsiveness and salt ion induction, are analyzed and summarized. Furthermore, the biomedical applications of peptide hydrogels, including bone tissue engineering, cell culture, antigen presentation, antibacterial materials, and drug delivery, are reviewed.
Review
Chemistry, Applied
Wen Li, Yanlong Zhan, Sirong Yu
Summary: This review presents the wide applications, general theories, and mechanisms of anti-icing of superhydrophobic coatings, focusing on the main impact factors and proposing functional coatings with unique properties as countermeasures. The current research status is overviewed, highlighting the existing problems and challenges for future development in this field.
PROGRESS IN ORGANIC COATINGS
(2021)
Article
Cell Biology
Leonardo Cristinziano, Remo Poto, Gjada Criscuolo, Anne Lise Ferrara, Maria Rosaria Galdiero, Luca Modestino, Stefania Loffredo, Amato de Paulis, Gianni Marone, Giuseppe Spadaro, Gilda Varricchi
Summary: The study found that immunoglobulin superantigens protein A and protein L can induce rapid release of histamine from HLMCs, while IL-33 does not trigger histamine release from lung mast cells. Prolonged incubation of HLMCs with superantigens and IL-33 leads to the release of VEGF-A and VEGF-C. Preincubation with IL-33 enhances the superantigenic release of histamine, angiogenic and lymphangiogenic factors from HLMCs.
Review
Chemistry, Physical
Lingchao Wang, Shuting Zhang, Yihe Zhang, Qi An
Summary: Piezodynamic therapy (PZDT) is a promising biomedical therapy for cancer elimination, antibacterial treatment, bone regeneration, and infected wound healing. However, the working principles of PZDT are still debated. This report documents and compares the dominant PZDT theories and discusses its biomedical applications. Future challenges and perspectives of PZDT research are also proposed.
Article
Chemistry, Physical
Atefe Rezaei, Fatemeh Rafieian, Safoura Akbari-Alavijeh, Mohammad Saeed Kharazmi, Seid Mahdi Jafari
Summary: Recent advances in emerging nanocarriers and stimuli-responsive (SR) delivery systems have revolutionized the food and pharmaceutical industries by allowing the release of encapsulated bioactive compounds upon external trigger. This controlled release mechanism is of great importance for targeted delivery in medicine and processing or storage of food products in the food industry.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2022)
Review
Infectious Diseases
Jenna Lanz, Nicholas Biniaz-Harris, Mara Kuvaldina, Samta Jain, Kim Lewis, Brian A. Fallon
Summary: This review discusses the potential clinical applications, associated risks, and challenges of disulfiram. It highlights the emerging data supporting its potential role in the treatment of addictions, infections, inflammatory conditions, neurological diseases, and cancers. However, caution is needed due to the range of side effects, and further animal studies and well-controlled clinical trials are required to assess its safety and efficacy for non-alcohol-related indications.
Review
Chemistry, Multidisciplinary
Zhongwei Yang, Jiawei Guo, Longwei Wang, Jian Zhang, Longhua Ding, Hong Liu, Xin Yu
Summary: This article provides an overview of the mechanisms and applications of nanozymes in electrochemical biosensors. It introduces the definition and characteristics of nanozymes, emphasizing their role in constructing efficient sensors. It discusses common categories of nanozyme materials and their applications in electrochemical biosensors. The article showcases the practical applications of nanozyme-based electrochemical biosensors in various domains and provides insights into the future development of this field.
Article
Engineering, Electrical & Electronic
Xiaojin Luo, Li Yang, Yue Cui
Summary: This review provides a comprehensive overview of the fundamental principles, manufacturing processes, and applications of micropumps. It is of great significance for the construction of next-generation micropumps and a wide range of biomedical applications, from disease diagnosis to drug delivery.
SENSORS AND ACTUATORS A-PHYSICAL
(2023)
Review
Cardiac & Cardiovascular Systems
Taija Makinen, Laurence M. Boon, Miikka Vikkula, Kari Alitalo
Summary: Lymphatic vessels play a crucial role in maintaining tissue fluid homeostasis and immune surveillance. Developmental or functional defects in lymphatic vessels can lead to lymphedema. Recent advancements have identified genetic causes and molecular pathways underlying lymphatic malformations and complex lymphatic anomalies, paving the way for targeted therapies.
CIRCULATION RESEARCH
(2021)
Article
Immunology
Sinem Karaman, Satu Paavonsalo, Krista Heinolainen, Madeleine H. Lackman, Amanda Ranta, Karthik A. Hemanthakumar, Yoshiaki Kubota, Kari Alitalo
Summary: Vascular endothelial growth factors (VEGFs) and their receptors (VEGFRs) play crucial roles in the development and maintenance of blood and lymphatic vessels. This study reveals that additional deletion of VEGFR1 or VEGFR3 exacerbates vascular regression induced by VEGFR2 deletion in certain organs, but not in others. The findings highlight previously unknown functions of VEGFR1 and VEGFR3 in endothelial cells.
JOURNAL OF EXPERIMENTAL MEDICINE
(2022)
Article
Cell & Tissue Engineering
Lars Muhl, Liqun He, Ying Sun, Maarja Andaloussi Mae, Riikka Pietila, Jianping Liu, Guillem Genove, Lei Zhang, Yuan Xie, Stefanos Leptidis, Giuseppe Mocci, Simon Stritt, Ahmed Osman, Andrey Anisimov, Karthik Amudhala Hemanthakumar, Markus Rasanen, Emil M. Hansson, Johan Bjorkegren, Michael Vanlandewijck, Klas Blomgren, Taija Makinen, Xiao-Rong Peng, Yizhou Hu, Patrik Ernfors, Thomas D. Arnold, Kari Alitalo, Urban Lendahl, Christer Betsholtz
Summary: ACE2 receptor is strongly expressed in pericytes in the CNS, heart, and pancreas, as well as in perineurial and adrenal fibroblasts, but not in endothelial cells. In the lung, ACE2 is expressed in bronchial epithelium and alveolar type II cells. The expression of ACE2 is organ-specific, with bronchial epithelium expressing it at birth, brain pericytes expressing it before birth, and heart pericytes expressing it after postnatal day 10.5. Understanding the vascular localization of ACE2 expression is crucial for interpreting data from mouse models of COVID-19 and may provide insights into the cause of vascular COVID-19 complications.
Article
Neurosciences
Arianna Merlini, Michael Haberl, Judith Strauss, Luisa Hildebrand, Nafiye Genc, Jonas Franz, Dmitri Chilov, Kari Alitalo, Cassandra Fluegel-Koch, Christine Stadelmann, Alexander Fluegel, Francesca Odoardi
Summary: In this study, using animal models and patients with multiple sclerosis, it was found that the involvement of meningeal layers in central nervous system autoimmunity is uneven. The leptomeninges were highly inflamed and showed structural changes, while the dura was marginally affected and largely excluded from autoimmune processes in the central nervous system.
NATURE NEUROSCIENCE
(2022)
Article
Peripheral Vascular Disease
Irene Matre Thowsen, Tore Reikvam, Trude Skogstrand, Anne-Maj Samuelsson, Dominik N. Muller, Olav Tenstad, Kari Alitalo, Tine Karlsen, Helge Wiig
Summary: Our results suggest that dermal lymphatic vessels are not involved in salt storage or blood pressure regulation in these mouse models of salt-sensitive hypertension.
Article
Biochemistry & Molecular Biology
Kathrin Barth, Verica Vasic, Brennan McDonald, Nora Heinig, Marc-Christoph Wagner, Ulrike Schumann, Cora Roehlecke, Frank Bicker, Lana Schumann, Konstantin Radyushkin, Jan Baumgart, Stefan Tenzer, Frauke Zipp, Matthias Meinhardt, Kari Alitalo, Irmgard Tegeder, Mirko H. H. Schmidt
Summary: Mature neurons, neural stem cells, and neural precursor cells secrete the neurovascular protein EGFL7 to shape the neurogenic niche of the hippocampus. Ablation of EGFL7 leads to increased neurogenesis and is correlated with elevated expression of VEGF-D. Infusion of VEGF-D increases neurogenesis, while its knockout decreases neurogenesis. Furthermore, EGFL7 knockout mice show improved spatial memory and memory consolidation in the hippocampus.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ivy K. N. Chiang, Matthew S. Graus, Nils Kirschnick, Tara Davidson, Winnie Luu, Richard Harwood, Keyi Jiang, Bitong Li, Yew Yan Wong, Mehdi Moustaqil, Emmanuelle Lesieur, Renae Skoczylas, Valerie Kouskoff, Jan Kazenwadel, Luis Arriola-Martinez, Emma Sierecki, Yann Gambin, Kari Alitalo, Friedmann Kiefer, Natasha L. Harvey, Mathias Francois
Summary: SOX7, a BEC-specific transcription factor, modulates the expression level of angiocrine signals in blood vascular endothelial cells (BECs) to pattern lymphatic vessels. It directly represses the transcription of Vegfc, a major lymphangiogenic growth factor, and interacts with HEY1 to regulate Notch pathway. Our finding reveals the importance of SOX7 in modulating downstream signaling events crucial for lymphatic patterning.
Article
Multidisciplinary Sciences
Nebeyu Yosef Gizaw, Pauliina Kallio, Tatjana Punger, Erika Gucciardo, Caj Haglund, Tom Bohling, Kaisa Lehti, Mika Sampo, Kari Alitalo, Riikka Kivela
Summary: This study reveals the critical role of PROX1 in RMS and its effects on cell clonogenicity, growth and tumor formation. FGFR-mediated signaling plays a key role in the growth effects of PROX1 in RMS. Inhibition of FGFR can suppress RMS cell proliferation and induce apoptosis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cardiac & Cardiovascular Systems
Anne-Maj Samuelsson, Theda Ulrike Patricia Bartolomaeus, Harithaa Anandakumar, Irene Thowsen, Elham Nikpey, Jianhua Han, Lajos Marko, Kenneth Finne, Olav Tenstad, Johannes Eckstein, Nikolaus Berndt, Titus Kuehne, Sarah Kedziora, Ibrahim Sultan, Trude Skogstrand, Tine Karlsen, Harri Nurmi, Sofia K. Forslund, Entela Bollano, Kari Alitalo, Dominik N. Muller, Helge Wiig
Summary: This study investigates the role of VEGF-B in cardiac proteomic and metabolic adaptation in heart failure, and finds that VEGF-B TG accelerates metabolic maladaptation, leading to structural cardiomyopathy and systolic heart failure.
CARDIOVASCULAR RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Katri Vaparanta, Anne Jokilammi, Ilkka Paatero, Johannes A. Merilahti, Juho Heliste, Karthik Amudhala Hemanthakumar, Riikka Kivela, Kari Alitalo, Pekka Taimen, Klaus Elenius
Summary: In this study, it is demonstrated that Neuregulin-1 (NRG-1) regulates myocardial growth through the STAT5b pathway. Disruption of the NRG-1/ERBB4 pathway or inhibition of Dynamin-2 reduces STAT5b activation and cardiomyocyte hypertrophy. Activation of Stat5 is also observed in NRG-1-induced myocardial growth in zebrafish embryos, and knockdown of stat5b leads to reduced myocardial growth and cardiac function. Furthermore, the NRG-1/ERBB4/STAT5b pathway is differentially regulated in patients with pathological cardiac hypertrophy.
Article
Immunology
Zhilin Li, Salli Antila, Harri Nurmi, Dmitri Chilov, Emilia A. Korhonen, Shentong Fang, Sinem Karaman, Britta Engelhardt, Kari Alitalo
Summary: The recent discovery of lymphatic vessels in the dura mater has potential implications for alternative therapeutics in CNS disorders. However, inhibiting the VEGF-C/VEGFR3 signaling pathway does not affect the development of CNS autoimmunity, suggesting that dural lymphatic vessels do not directly contribute to CNS autoimmunity.
SCIENCE IMMUNOLOGY
(2023)
Article
Biochemical Research Methods
Aino Sormunen, Emma Koivulehto, Kari Alitalo, Kalle Saksela, Nihay Laham-Karam, Seppo Ylae-Herttuala
Summary: Traditional Western blotting is commonly used in biological research but has issues with time and reproducibility. Automation, including semi-automated and fully automated systems, offers time-saving and sensitivity advantages. However, the cost of devices and reagents is a downside. Nevertheless, automation can be beneficial for increasing output and facilitating sensitive protein analyses.
METHODS AND PROTOCOLS
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
Anna Kemppi, Lea M. Rahtu-Korpela, Zoltan Szabo, Sami Raatikainen, Andrey Anisimov, Kari K. Alitalo, Johanna Magga, Risto Kerkela
Article
Medicine, Research & Experimental
Emilia A. Korhonen, Aino Murtomaki, Sawan Kumar Jha, Andrey Anisimov, Anne Pink, Yan Zhang, Simon Stritt, Inam Liaqat, Lukas Stanczuk, Laura Alderfer, Zhiliang Sun, Emmi Kapiainen, Abhishek Singh, Ibrahim Sultan, Anni Lantta, Veli-Matti Leppanen, Lauri Eklund, Yulong He, Hellmut G. Augustin, Kari Vaahtomeri, Pipsa Saharinen, Taija Makinen, Kari Alitalo
Summary: This study reveals the involvement of Ang2 in VEGF-C-induced lymphangiogenesis through the PI3K/Akt signaling pathway in lymphatic endothelial cells. Blockade of Ang2 or deletion of Tie receptors in LECs inhibits lymphangiogenesis. The study highlights the important crosstalk between VEGF-C and Ang signaling pathways and suggests potential therapeutic approaches targeting Ang2/Tie/PI3K signaling for lymphangiogenesis.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Respiratory System
Masahiro Yamashita, Miyuki Niisato, Yasushi Kawasaki, Sinem Karaman, Marius R. Robciuc, Yuji Shibata, Yoji Ishida, Ryosuke Nishio, Tomoyuki Masuda, Tamotsu Sugai, Masao Ono, Rubin M. Tuder, Kari Alitalo, Kohei Yamauchi
Summary: This study investigates the role of vascular endothelial growth factor (VEGF)-C/VEGF receptor 3 (VEGFR-3) signaling in the recovery process of lung injury. The results show that VEGF-C/VEGFR-3 signaling can alleviate lung injury and promote macrophage phagocytosis.
EUROPEAN RESPIRATORY JOURNAL
(2022)
