Article
Biochemistry & Molecular Biology
Hanin Hajouj, Ali Khattib, Dana Atrahimovich, Sanaa Musa, Soliman Khatib
Summary: Protein S-nitrosylation is involved in cellular regulation mechanisms and impaired S-nitrosylation is associated with various pathophysiological conditions. The study found that S-nitrosylation improves the activities, antioxidant capacity, and penetration ability of the enzyme PON1, which may positively affect atherosclerosis disease progression.
Article
Biochemistry & Molecular Biology
Benjamin Lau, Hossein Fazelinia, Ipsita Mohanty, Serena Raimo, Margarita Tenopoulou, Paschalis-Thomas Doulias, Harry Ischiropoulos
Summary: This study identified and analyzed S-nitrosocysteine residues in mouse heart proteins, with a significant enrichment of these modifications in mitochondrial proteins participating in metabolism. The major functional pathways impacted by protein S-nitrosylation include regulatory proteins in glycolysis, TCA cycle, oxidative phosphorylation, and ATP production. The meta-analysis of the proteomic data supports the hypothesis that nitric oxide signaling via protein S-nitrosylation is a regulator of cardiomyocyte metabolism to ensure optimal cardiac function.
Review
Chemistry, Analytical
Xin Wang, Wenxiu Zhou, Zhenye Gao, Xiaoyuan Lv
Summary: This review summarizes the application of mass spectrometry techniques in the analysis of protein S-nitrosylation, including sample preparation techniques and qualitative and quantitative strategies. The roles of protein S-nitrosylation in human diseases are discussed, with an emphasis on current limitations and future research directions.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Plant Sciences
Jiali Zhai, Yuanlin Liang, Senlin Zeng, Jinping Yan, Kunzhi Li, Huini Xu
Summary: In tomato plants, S-nitrosylation dependent on nitric oxide is crucial for the function of glutathione reductase under NaCl stress. It enhances salt tolerance by increasing the transcripts, enzyme activity, and S-nitrosylated level of GR, while regulating the oxidative state.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Plant Sciences
Xuewei Song, Ting Wang, Yang Zhang, Jing-Quan Yu, Xiao-Jian Xia
Summary: S-nitrosoglutathione reductase (GSNOR) plays a critical role in regulating plant stress tolerance, especially under high temperature conditions. Suppressing GSNOR expression reduces the thermotolerance of tomato plants, which is associated with decreased accumulation of abscisic acid (ABA), salicylic acid (SA), activated mitogen-activated protein kinase (MAPK), and heat shock protein. GSNOR is also involved in the upregulation of RESPIRATORY BURST OXIDASE HOMOLOG1 (RBOH1) and apoplastic H2O2 production in response to high temperature. Maintaining a balanced interaction between S-nitrosothiol (SNO) and H2O2 is crucial for cellular redox homeostasis and thermotolerance.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Medicine, Research & Experimental
Zu-Cheng Shen, Jian-Min Liu, Jie-Yan Zheng, Meng-Die Li, Dan Tian, Yue Pan, Wu-Cheng Tao, Shuang-Qi Gao, Zhi-Xuan Xia
Summary: Protein palmitoylation/nitrosylation crosstalk plays a critical role in regulating anxiety behavior and may serve as a potential target for anxiolytic intervention.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Cardiac & Cardiovascular Systems
Shathiyah Kulandavelu, Raul A. Dulce, Christopher Murray, Michael A. Bellio, Julia Fritsch, Rosemeire Kanashiro-Takeuchi, Himanshu Arora, Ellena Paulino, Daniel Soetkamp, Wayne Balkan, Jenny E. Van Eyk, Joshua M. Hare
Summary: The deficiency of GSNOR can lead to imbalanced placental S-nitrosylation and induce preeclampsia in mice, characterized by symptoms such as hypertension, proteinuria, and renal pathology. Ascorbate can reverse these pathological changes, except for fetal weight. The decreased GSNOR activity and increased nitrosative stress are also found in human preeclamptic placentas.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2022)
Article
Cell Biology
Weiwei Yi, Yuying Zhang, Bo Liu, Yuanyuan Zhou, Dandan Liao, Xinhua Qiao, Dan Gao, Ting Xie, Qin Yao, Yao Zhang, Yugang Qiu, Gang Huang, Zhiyang Chen, Chang Chen, Zhenyu Ju
Summary: NO plays a critical role in HSC regeneration, and the deletion of GSNOR leads to protein aggregation and activation of UPR. Treatment with TCA and deletion of Chop can correct these issues in Gsnor(-/-) HSCs.
Article
Biochemistry & Molecular Biology
Oscar Arriagada Castillo, Gustavo Herrera, Carlos Manriquez, Andrea F. Rojas, Daniel R. Gonzalez
Summary: The cardioprotective effects of nitric oxide are mediated through S-nitrosylation of mitochondrial proteins, and inhibition of GSNOR with C2 leads to increased S-nitrosylation levels, reducing myocardial damage and improving post-ischemia ventricular function. These findings suggest that GSNOR may be a promising pharmacological target for cardiac reperfusion injury.
Review
Biochemistry & Molecular Biology
Sofia Vrettou, Brunhilde Wirth
Summary: Redox post-translational modifications, such as S-glutathionylation and S-nitrosylation, play crucial roles in regulating protein function, localization, activity, and structure. These modifications are controlled by fluctuations in reactive oxygen and nitrogen species and have significant impacts on mitochondrial processes, including bioenergetics, morphology, metabolism, and apoptosis. Dysregulation of S-glutathionylation and S-nitrosylation is associated with neurodegenerative disorders such as Alzheimer's, Parkinson's, amyotrophic lateral sclerosis, and Friedreich's ataxia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Edoardo Jun Mattioli, Jacopo Rossi, Maria Meloni, Marcello De Mia, Christophe H. Marchand, Andrea Tagliani, Silvia Fanti, Giuseppe Falini, Paolo Trost, Stephane D. Lemaire, Simona Fermani, Matteo Calvaresi, Mirko Zaffagnini
Summary: S-nitrosylation plays an important role in cellular signaling by modulating protein function and conformation. This study investigates the molecular mechanisms of GSNO-dependent thiol oxidation using CrGAPA. The research reveals that GSNO induces reversible enzyme inhibition through S-nitrosylation, particularly at the catalytic Cys149, leading to CrGAPA inactivation.
Review
Biochemistry & Molecular Biology
Elena Kalinina, Maria Novichkova
Summary: S-glutathionylation and S-nitrosylation are reversible post-translational modifications on the cysteine thiol groups of proteins, important for regulating protein functional activity and cellular processes. The connection and switch functions between these modifications are performed by GSNO, dependent on factors like GSH content, GSH/GSSG ratio, and cellular redox state. Trx family enzymes play a crucial role in regulating these processes, with their activity determined by cellular redox status and GSH/GSSG ratio.
Review
Biochemistry & Molecular Biology
Ajanta Chatterji, Rajib Sengupta
Summary: Nitric oxide is a gasotransmitter molecule that plays various roles in cellular processes. Recent research supports the existence of S-nitrosylated proteins within cells, challenging the controversy over their stability. Evidence suggests that these proteins directly regulate functionality and provide insights into cell signaling.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Hyang-Mi Lee, Ji Woong Choi, Min Sik Choi
Summary: Ischemia-reperfusion injury (IRI) is a damage process induced in hypoxic tissue when oxygen is supplied after ischemia. Restoration of reduced nitric oxide (NO) levels may alleviate reperfusion injury in ischemic organs, with the protective mechanism due to anti-inflammatory and antioxidant effects of NO, as well as regulation of cell signaling pathways. S-nitrosylation (SNO) mediates the detrimental or protective effect of NO depending on the nitrosylated target protein, which also plays a role in the IRI process.
Article
Biochemistry & Molecular Biology
Divya Seth, Colin T. Stomberski, Precious J. McLaughlin, Richard T. Premont, Kathleen Lundberg, Jonathan S. Stamler
Summary: The aim of this study was to investigate the interactions between nitric oxide synthase (NOS) isoforms and proteins, as well as the mechanisms by which they mediate S-nitrosylation. The results showed that each NOS isoform interacts with and S-nitrosylates many proteins, but a large proportion of S-nitrosylated proteins are not associated with NOS. Additionally, the interactomes and S-nitrosylomes of individual NOS isoforms are largely unique, indicating specific roles for each isoform in S-nitrosylation.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Cardiac & Cardiovascular Systems
Kei Hayashida, Aranya Bagchi, Yusuke Miyazaki, Shuichi Hirai, Divya Seth, Michael G. Silverman, Emanuele Rezoagli, Eizo Marutani, Naohiro Mori, Aurora Magliocca, Xiaowen Liu, Lorenzo Berra, Allyson G. Hindle, Michael W. Donnino, Rajeev Malhotra, Matthews O. Bradley, Jonathan S. Stamler, Fumito Ichinose
Article
Biochemistry & Molecular Biology
Colin T. Stomberski, Hua-Lin Zhou, Liwen Wang, Focco van den Akker, Jonathan S. Stamler
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Multidisciplinary Sciences
Hua-Lin Zhou, Rongli Zhang, Puneet Anand, Colin T. Stomberski, Zhaoxia Qian, Alfred Hausladen, Liwen Wang, Eugene P. Rhee, Samir M. Parikh, S. Ananth Karumanchi, Jonathan S. Stamler
Correction
Multidisciplinary Sciences
Hua-Lin Zhou, Rongli Zhang, Puneet Anand, Colin T. Stomberski, Zhaoxia Qian, Alfred Hausladen, Liwen Wang, Eugene P. Rhee, Samir M. Parikh, S. Ananth Karumanchi, Jonathan S. Stamler
Letter
Cardiac & Cardiovascular Systems
James D. Reynolds, Richard T. Premont, Jonathan S. Stamler
Review
Cardiac & Cardiovascular Systems
Richard T. Premont, James D. Reynolds, Rongli Zhang, Jonathan S. Stamler
CIRCULATION RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Colin T. Stomberski, Puneet Anand, Nicholas M. Venetos, Alfred Hausladen, Hua-Lin Zhou, Richard T. Premont, Jonathan S. Stamler
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Divya Seth, Alfred Hausladen, Jonathan S. Stamler
ANTIOXIDANTS & REDOX SIGNALING
(2020)
Article
Biochemistry & Molecular Biology
Richard T. Premont, James D. Reynolds, Rongli Zhang, Jonathan S. Stamler
Summary: Red blood cell-mediated vasodilation is crucial for oxygen delivery, facilitated by the conversion of heme-bound nitric oxide into vasodilator S-nitrosothiol. This process is regulated by a strictly conserved Cys residue in hemoglobin. The model of a three-gas respiratory cycle (O-2/NO/CO2) is supported by the coupling of hemoglobin allostery to NO conversion and SNO release.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Article
Biochemistry & Molecular Biology
Min-Kyoo Shin, Edwin Vazquez-Rosa, Yeojung Koh, Matasha Dhar, Kalyani Chaubey, Coral J. Cintron-Perez, Sarah Barker, Emiko Miller, Kathryn Franke, Maria F. Noterman, Divya Seth, Rachael S. Allen, Cara T. Motz, Sriganesh Ramachandra Rao, Lara A. Skelton, Machelle T. Pardue, Steven J. Fliesler, Chao Wang, Tara E. Tracy, Li Gan, Daniel J. Liebl, Jude P. J. Savarraj, Glenda L. Torres, Hilda Ahnstedt, Louise D. McCullough, Ryan S. Kitagawa, H. Alex Choi, Pengyue Zhang, Yuan Hou, Chien-Wei Chiang, Lang Li, Francisco Ortiz, Jessica A. Kilgore, Noelle S. Williams, Victoria C. Whitehair, Tamar Gefen, Margaret E. Flanagan, Jonathan S. Stamler, Mukesh K. Jain, Allison Kraus, Feixiong Cheng, James D. Reynolds, Andrew A. Pieper
Summary: Traumatic brain injury induces tau acetylation, leading to neurodegeneration and neurobehavioral impairment. Protecting mice from TBI can be achieved by blocking GAPDH S-nitrosylation, inhibiting p300/CBP, or stimulating Sirtuin1. Ac-tau may serve as a therapeutic target and potential blood biomarker of TBI.
Review
Endocrinology & Metabolism
Hua-Lin Zhou, Richard T. Premont, Jonathan S. Stamler
Summary: This review discusses the crucial role of S-nitrosylation in the regulation of insulin signaling throughout the insulin life cycle, impacting both metabolic health and disease. The interplay between insulin signaling and protein S-nitrosylation is highlighted, with aberrant S-nitrosylation contributing to metabolic disorders such as type 2 diabetes mellitus. Understanding the impact of S-nitrosylation on cellular metabolism could provide valuable insights in the field of metabolic signaling and insulin-related disorders.
NATURE REVIEWS ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Megan Pophal, Zachary W. Grimmett, Clara Chu, Seunghee Margevicius, Thomas Raffay, Kristie Ross, Anjum Jafri, Olivia Giddings, Jonathan S. Stamler, Benjamin Gaston, James D. Reynolds
Summary: The study suggests that F-ENO could reflect airway S-nitrosothiol concentrations and serve as a biomarker for asthma. Inhalational challenge with GSNO resulted in increased F-ENO, indicating a potential non-invasive test for monitoring airway GSNO breakdown.
Article
Biochemistry & Molecular Biology
Divya Seth, Colin T. Stomberski, Precious J. McLaughlin, Richard T. Premont, Kathleen Lundberg, Jonathan S. Stamler
Summary: The aim of this study was to investigate the interactions between nitric oxide synthase (NOS) isoforms and proteins, as well as the mechanisms by which they mediate S-nitrosylation. The results showed that each NOS isoform interacts with and S-nitrosylates many proteins, but a large proportion of S-nitrosylated proteins are not associated with NOS. Additionally, the interactomes and S-nitrosylomes of individual NOS isoforms are largely unique, indicating specific roles for each isoform in S-nitrosylation.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Biochemical Research Methods
Puneet Seth, Richard T. Premont, Jonathan S. Stamler
Summary: Post-translational modification by S-nitrosylation plays a key role in regulating cellular functions and impacting proteins across phylogeny. The protocol described focuses on isolating S-nitrosylated proteins from C. elegans, with potential for adaptation to mammalian tissues. Protecting SNO proteins from degradation remains a major challenge in this protocol.
Article
Biochemistry & Molecular Biology
Claudia Cirotti, Salvatore Rizza, Paola Giglio, Noemi Poerio, Maria Francesca Allega, Giuseppina Claps, Chiara Pecorari, Ji-Hoon Lee, Barbara Benassi, Daniela Barila, Caroline Robert, Jonathan S. Stamler, Francesco Cecconi, Maurizio Fraziano, Tanya T. Paull, Giuseppe Filomeni
Summary: This study demonstrates that GSNOR is induced at the translational level in response to hydrogen peroxide and mitochondrial ROS, and that the modulation of ATM/GSNOR axis affects cell survival and mitophagy. This interplay also regulates T-cell activation, providing new insights into the pleiotropic effects of ATM in the context of immune function.
