Article
Biochemistry & Molecular Biology
Shuai Wang, Hongjie Qiao, Pengxiang Wang, Yuan Wang, Danian Qin
Summary: Zdhhc19 knockout mice did not show disruption in spermatogenesis but exhibited male infertility with defects in sperm morphology and functions, highlighting the essential role of Zdhhc19 in sperm production and fertility in mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Reproductive Biology
Camilo Hernandez-Aviles, Luisa Ramirez-Agamez, Dickson D. Varner, Charles C. Love
Summary: The process of fertilization involves acrosomal exocytosis (AE) as the interaction between sperm and oocyte. While the role of sperm acrosome in fertilization has been extensively studied, there is limited knowledge about the molecular mechanisms governing acrosomal function in non-mice or human species. Evaluating sperm acrosomal function should be a routine test for individuals selected for breeding potential, even though acrosomal dysfunction-related subfertility is less common in large animals. High throughput technologies such as flow cytometry and mass spectrometry-based proteomic analysis can be used to study acrosomal function and discover new markers of acrosomal dysfunction in stallions.
Article
Developmental Biology
Gabriela Carrasquel Martinez, Andres Aldana, Jorge Carneiro, Claudia Lydia Trevino, Alberto Darszon
Summary: Mammalian sperm capacitation, involving biochemical and physiological modifications, is a crucial step for successful fertilization. This study showed that the acrosome pH gradually increases during human sperm capacitation, and the V-ATPase plays a key role in regulating the acrosome acidity. These findings provide important insights into the factors influencing sperm fusion and fertilization.
MOLECULAR HUMAN REPRODUCTION
(2022)
Article
Genetics & Heredity
Jiaxiong Wang, Hui Tang, Qinyan Zou, Aiyan Zheng, Hong Li, Shenmin Yang, Jingjing Xiang
Summary: This study identified a homozygous pathogenic CATSPER3 mutation in a male infertility patient from a consanguineous family, revealing its impact on assisted reproductive technology outcome. The mutation caused failure of sperm acrosome reaction without affecting routine semen parameters. Successful treatment was achieved through ICSI.
MOLECULAR GENETICS & GENOMIC MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Shehreen Amjad, Md Saidur Rahman, Won-Ki Pang, Do-Yeal Ryu, Elikanah Olusayo Adegoke, Yoo-Jin Park, Myung-Geol Pang
Summary: In this study, exposure to a phthalate mixture resulted in decreased sperm motility and hyperactivity, compromised sperm ability to generate ATP, induced abnormal capacitation and acrosome reaction, upregulated protein tyrosine phosphorylation via a protein kinase-A-dependent pathway, and significantly affected fertilization and early embryonic development. The findings suggest that the phthalate mixtures adversely affected sperm processes and ultimately led to poor fertilization rates and repressed embryonic development, with the lowest observed adverse effect dose being <1 µg/mL in vitro.
Article
Genetics & Heredity
Chao Li, Ruifang Yu, Huan Liu, Jiashan Qiao, Fenghao Zhang, Shumei Mu, Mingshen Guo, Han Zhang, Yanqin Li, Xianjiang Kang
Summary: This study investigates the function of mitochondrial proteins in acrosome formation in Eriocheir sinensis sperm. By comparing different inducers and observing morphological changes, it explores the role of mitochondrial proteins in acrosome formation. LC-MS/MS analysis reveals a total of 945 proteins released during the acrosome reaction, with 17.57% derived from mitochondria. Immunofluorescence analysis confirms the independent involvement of malate dehydrogenase and voltage-dependent anion channel 3 in acrosomal membrane formation.
Article
Reproductive Biology
Haolin Zhang, Hong Liu, Sachi Kataoka, Masashi Kinukawa, Kyoko Uchiyama, Jun Kambe, Gen Watanabe, Wanzhu Jin, Kentaro Nagaoka
Summary: The study found that the L-amino acid oxidase encoded by the Lao1 gene plays an important role in male reproduction, with the lack of this gene resulting in fewer embryos and offspring in male mice. Additionally, deficiency in LAO1 in sperm may cause male subfertility, as its localization in the sperm acrosome affects sperm viability and morphology.
BIOLOGY OF REPRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Roland Abi Nahed, Magali Dhellemmes, Christine Payre, Emilie Le Blevec, Jean-Philippe Perrier, Sylviane Hennebicq, Jessica Escoffier, Pierre F. Ray, Corinne Loeuillet, Gerard Lambeau, Christophe Arnoult
Summary: The secreted phospholipase A2 (PLA2G10) plays an important role in mouse fertilization by enhancing acrosome reaction and secretion, improving fertilization rate, and binding to PLA2R1 on gametes. It is shown that the catalytic activity and PLA2R1 binding of PLA2G10 both contribute to its profertility effect, and the study provides new insights into the complexity of PLA2G10's action in optimizing fertilization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Naila Umer, Lena Arevalo, Sharang Phadke, Keerthika Lohanadan, Gregor Kirfel, Dominik Sons, Denise Sofia, Walter Witke, Hubert Schorle
Summary: Profilin (PFN) proteins play crucial roles in actin polymerization and are encoded by four Pfn genes in mice and humans. PFNs, particularly PFN3, are essential for spermiogenesis, sperm morphology, and fertility in males. Lack of PFN3 leads to abnormal acrosome formation, reduced sperm motility, and dysregulation of autophagy, ultimately impacting male reproductive function.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Mina Pencheva, Donka Keskinova, Pavel Rashev, Yvetta Koeva, Nina Atanassova
Summary: Testicular angiotensin converting enzyme (ACE) plays a crucial role in male reproduction and fertility. Research shows that the proper distribution of tACE in the neck and mid-piece of sperm is essential for normal sperm motility. Different patterns of tACE expression were observed in pathological semen samples compared to normospermia.
Article
Reproductive Biology
Z. Bowker, S. Goldstein, H. Breitbart
Summary: Sperm cells must undergo the acrosome reaction in order to penetrate the egg. Capacitation, a series of biochemical changes, is necessary for this process to occur. This study discusses the role of protein acetylation in protecting bovine sperm from premature acrosome reaction. The results suggest a PKA-independent and EPAC-CaMKII dependent mechanism involving hyperacetylation to prevent acrosome reaction.
Article
Biochemistry & Molecular Biology
Masaya Koganesawa, Munehiro Yamaguchi, Sachin K. Samuchiwal, Barbara Balestrieri
Summary: Macrophages activated by IL-4 (M2) or LPS+IFN gamma (M1) have distinct lipid metabolism profiles, with Pla2g5 playing a key role in this process. Pla2g5 is involved in phospholipid metabolism and eicosanoid production in both types of activated macrophages.
Article
Environmental Sciences
Anandha Rao Ravula, Suresh Yenugu
Summary: Studies on unintentional intake of pyrethroid pesticides similar to actual human exposure settings are rare. Exposure to low and high doses of pyrethroid mixture for 15 months resulted in abnormal lipid profile, liver function, and antioxidant enzyme activities, as well as damage to anatomical architecture in various tissues. This exposure also led to decreased sperm count, enzyme activities, impaired capacitation and acrosome reaction, and perturbations in gene expression related to male gamete production.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Review
Biochemistry & Molecular Biology
Cameron C. Gardner, Paul F. James
Summary: Na+/H+ exchangers (NHEs) are important regulators of pH in eukaryotic cells and play crucial roles in sperm function and male fertility. Mammalian sperm express multiple NHE isoforms that contribute to various aspects of sperm physiology and development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Renzheng Zhang, Xian Guo, Chunnian Liang, Jie Pei, Pengjia Bao, Mancai Yin, Fude Wu, Min Chu, Ping Yan
Summary: This study used a quantitative proteomic profiling approach to analyze the protein level changes in frozen-thawed Ashidan yak spermatozoa during fertilization. The results revealed differential protein abundances during sperm capacitation and acrosome reaction induction, with enrichment in different signaling pathways. Superoxide dismutase 1 and heat shock protein 90 alpha were identified as regulators of sperm capacitation and acrosome reaction.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Lea Rethore, Joohee Park, Jerome Montnach, Sebastien Nicolas, Joseph Khoury, Elodie Le Seac'h, Kamel Mabrouk, Harold De Pomyers, Helene Tricoire-Leignel, Cesar Mattei, Daniel Henrion, Ziad Fajloun, Michel De Waard, Claire Legendre, Christian Legros
Summary: This study investigates the interaction between Na+ and Ca2+ channels in GH3b6 cells, revealing a crosstalk between Na-V and LTCC and providing new insights into the mode of action of neurotoxins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Jerome Montnach, Laila Ananda Blomer, Ludivine Lopez, Luiza Filipis, Herve Meudal, Aude Lafoux, Sebastien Nicolas, Duong Chu, Cecile Caumes, Remy Beroud, Chris Jopling, Frank Bosmans, Corinne Huchet, Celine Landon, Marco Canepari, Michel De Waard
Summary: The researchers developed a photoactivatable toxin targeting ion channels and demonstrated its potential in precise control of neuronal channel function through in vitro and in vivo experiments.
NATURE COMMUNICATIONS
(2022)
Article
Neurosciences
Alexandra Tran-Van-Minh, Michel De Waard, Norbert Weiss
Summary: Voltage-gated calcium channels are crucial for regulating brain function by allowing calcium entry into neurons. The proper functioning of these channels relies on the co-assembly of a pore-forming subunit (Ca-v alpha(1)) with ancillary subunits, with Ca-v beta playing a vital role in regulating surface expression and gating of the channels. In this study, the importance of surface charged residues in Ca-v beta(3) in the regulation of Ca(v)2.1 channels was investigated, revealing their contribution to channel gating and suggesting additional contacts beyond the known interaction between Ca-v alpha(1) and Ca-v beta.
Article
Biochemistry & Molecular Biology
Roland Abi Nahed, Magali Dhellemmes, Christine Payre, Emilie Le Blevec, Jean-Philippe Perrier, Sylviane Hennebicq, Jessica Escoffier, Pierre F. Ray, Corinne Loeuillet, Gerard Lambeau, Christophe Arnoult
Summary: The secreted phospholipase A2 (PLA2G10) plays an important role in mouse fertilization by enhancing acrosome reaction and secretion, improving fertilization rate, and binding to PLA2R1 on gametes. It is shown that the catalytic activity and PLA2R1 binding of PLA2G10 both contribute to its profertility effect, and the study provides new insights into the complexity of PLA2G10's action in optimizing fertilization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Letter
Pharmacology & Pharmacy
Luay Alkazmi, Hayder M. Al-kuraishy, Gaber El-Saber Batiha, Gomaa Mostafa-Hedeab, Michel De Waard, Jean-Marc Sabatier, Saeed M. Kabrah, Hebatallah M. Saad, Ali I. Al-Gareeb, Jesus Simal-Gandara
Review
Immunology
Ahmad O. Babalghith, Hayder M. Al-Kuraishy, Ali Al-Gareeb, Michel De Waard, Sadiq Mohammed Al-Hamash, Sabatier Jean-Marc, Walaa A. Negm, Gaber El-Saber Batiha
Summary: Berberine (BBR) has potential anti-inflammatory, antioxidant, antiviral, and immune-regulatory effects, making it a prospective candidate against SARS-CoV-2. Studies have shown that BBR can mitigate inflammatory changes associated with Covid-19 and reduce the risk of ALI/ARDS in patients.
INFLAMMOPHARMACOLOGY
(2022)
Review
Neurosciences
Nawal Alsubaie, Hayder M. Al-Kuraishy, Ali Al-Gareeb, Bandar Alharbi, Michel De Waard, Jean-Marc Sabatier, Hebatallah M. Saad, Gaber El-Saber Batiha
Summary: This study is a narrative review on the effects of statins on Alzheimer's disease (AD), and it found that the use of statins may have detrimental effects on cognitive functions in the elderly, but there is currently no conclusive evidence regarding the effects of statins on cognitive functions in AD patients.
Review
Medicine, General & Internal
Ahmad O. Babalghith, Hayder M. Al-Kuraishy, Ali Al-Gareeb, Michel De Waard, Jean-Marc Sabatier, Hebatallah M. Saad, Gaber El-Saber Batiha
Summary: COVID-19 is caused by SARS-CoV-2 variants and is characterized by hyperinflammation, oxidative stress, multi-organ injury, acute lung injury, and acute respiratory distress syndrome. GDF15, a potential biomarker, plays a vital role in controlling cell growth, apoptosis, and inflammatory activation. Higher expression of GDF15 in COVID-19 may be a compensatory mechanism against dysregulated inflammatory reactions.
Article
Agriculture, Dairy & Animal Science
Helmy A. A. Torky, Hebatallah M. Saad, Samy A. Khaliel, Asmaa T. Kassih, Jean-Marc Sabatier, Gaber El-Saber Batiha, Helal F. Hetta, Eman M. Elghazaly, Michel De Waard
Summary: Corynebacterium pseudotuberculosis (C. pseudotuberculosis) is a causative agent of chronic diseases in animals and humans, with poor therapeutic response. A study analyzed 120 samples and found only 10% positive for C. pseudotuberculosis. Genetic analysis showed intra-subtyping variation. The study also identified immune expression patterns in affected lymph nodes, indicating the involvement of the NF-kappa B/p65 signaling pathway in the inflammatory response to control the pathogen.
Article
Microbiology
Attia A. Abou Zaid, Rehab Abd R. El Maged, Nesma Rasheed, Dina Mohamed Mansour, Heba H. Mahboub, Hany M. Abd El-Lateef, Jean-Marc Sabatier, Hebatallah M. Saad, Gaber El-Saber Batiha, Michel De Waard
Summary: This study aims to verify the ability of the copepod Lernanthropus kroyeri to uptake and accumulate heavy metals by examining its prevalence, clinical signs, and post-mortem changes in sea bass. The results showed that L. kroyeri could accumulate a high amount of zinc in its tissues and cause histopathological alterations in fish. This highlights the potential use of L. kroyeri as a bio-indicator for metal monitoring in aquaculture.
Article
Chemistry, Medicinal
Walaa Yehia Abdelzaher, Michel De Waard, Alyaa Abdelfattah Abdelmonaem, Dalia Mohamed Ali, Nashwa Fathy Gamal El-Tahawy, Rehab Ahmed Rifaai, Hatem A. Mohamed, Kareem Shaheen, Mohamed Ahmed Zeen El-Din, Nermeen N. Welson, Shereen ELsayed Tawfeek, Gaber El-Saber Batiha, Asmaa Mohamed Abdel-Aziz
Summary: The present experiment aimed to identify the potential protective role of empagliflozin (EMPA) on haloperidol (HAL)-induced ovarian damage in female rats because of its anti-inflammatory, antioxidant, and antiapoptotic effects. The results showed that EMPA significantly alleviated HAL-induced ovarian toxicity by modulating the Sirt-1/Hsp70/TNF-alpha/caspase-3 signaling pathway.
Article
Biochemistry & Molecular Biology
Duaa Eliwa, Amal Kabbash, Mona El-Aasr, Haytham O. Tawfik, Gaber El-Saber Batiha, Mohamed H. Mahmoud, Michel De Waard, Wagdy M. Eldehna, Abdel-Rahim S. Ibrahim
Summary: This study explores the bioconversion of the natural heterocyclic alkaloid papaverine using filamentous fungi and investigates the antidiabetic potential of papaverine and its metabolites through molecular docking simulations. Seven metabolites were isolated from the biotransformation process, and their structures were elucidated using NMR and mass spectroscopy. Molecular docking studies showed that metabolite 7 exhibited better binding interactions with target enzymes and displayed inhibition of alpha-glucosidase and lipase enzymes comparable to known therapeutic agents.
Article
Cell Biology
Barbara B. R. Oliveira-Mendes, Malak Alameh, Beatrice Ollivier, Jerome Montnach, Nicolas Bidere, Frederique Souaze, Nicolas Escriou, Flavien Charpentier, Isabelle Baro, Michel De Waard, Gildas Loussouarn
Summary: Controversial reports have suggested that SARS-CoV E and 3a proteins are not viroporins expressed at the plasma membrane. Expressing these proteins in CHO cells induces cell death and changes in cell morphology. Outwardly rectifying currents observed in detached cells are most likely conducted by Pannexin channels activated by cell morphology changes and potentially cell death.
Letter
Oncology
Barbara B. R. Oliveira-Mendes, Malak Alameh, Jerome Montnach, Beatrice Ollivier, Solene Gibaud, Sylvain Feliciangeli, Florian Lesage, Flavien Charpentier, Gildas Loussouarn, Michel De Waard, Isabelle Baro
CLINICAL AND TRANSLATIONAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Antoine Persello, Fouzia Souab, Thomas Dupas, Virginie Aillerie, Edith Bigot, Manon Denis, Angelique Erraud, Thomas Pele, Angelique Blangy-Letheule, Pierre Miniou, Philippe Guedat, Michel De Waard, Emmanuelle Abgueguen, Bertrand Rozec, Benjamin Lauzier
Summary: This study aimed to develop a rat model of extracorporeal circulation (ECC) and establish its clinical relevance. The results demonstrated that the rats exhibited hypotension, hyperlactatemia, and behavioral alterations after ECC, similar to human patients. Blood biomarkers and transcriptomic analysis also showed similarities in the ECC response between humans and rats. Therefore, this new rat model appears to be a cost-effective preclinical model of human ECC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)