Article
Engineering, Biomedical
Zhen Liu, Junfei Huang, Deni Kang, Yi Zhou, Lijuan Du, Qian Qu, Jin Wang, Lihong Wen, Danlan Fu, Zhiqi Hu, Yong Miao
Summary: An optimized 3-D co-culture system was developed to restore the hair-inductive characteristics of human dermal papilla cells (hDPCs) by mimicking the in-vivo microenvironment. The study showed that Matrigel-encapsulated hDPCs spontaneously formed hDPC aggregates (hDPAs) with better activity, higher proliferation rates, and less apoptosis and hypoxia. The co-culture with hair matrix cells and dermal sheath cup cells further enhanced the expression of hair regeneration-related genes and improved mature hair follicle induction.
ACTA BIOMATERIALIA
(2023)
Article
Multidisciplinary Sciences
Sara G. Romeo, Ilaria Secco, Edoardo Schneider, Christina M. Reumiller, Celio X. C. Santos, Anna Zoccarato, Vishal Musale, Aman Pooni, Xiaoke Yin, Konstantinos Theofilatos, Silvia Cellone Trevelin, Lingfang Zeng, Giovanni E. Mann, Varun Pathak, Kevin Harkin, Alan W. Stitt, Reinhold J. Medina, Andriana Margariti, Manuel Mayr, Ajay M. Shah, Mauro Giacca, Anna Zampetaki
Summary: The microvasculature is critical for the delivery of oxygen and metabolites throughout tissues. In this study, human blood vessel organoids (BVOs) were used to show that CTGF is a critical paracrine regulator of microvascular integrity that can restore pericyte coverage and vessel structure.
NATURE COMMUNICATIONS
(2023)
Article
Engineering, Biomedical
Ruth Jinfen Chai, Wan Ling Wong, Cyrus Weijie Beh
Summary: Patients with severe burns require rapid intervention to prevent hypothermia, infection, and fluid loss. Current treatments involve surgical excision of burned skin and reconstruction with skin autografts. However, alternative treatments such as cultured epithelial autografts and spray-on skin have their own challenges. Bioprinting technology has been explored to fabricate skin grafts using collagen-based bioink and appropriate cell types.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Multidisciplinary Sciences
Stefan Andreas Zambach, Changsi Cai, Hans Christian Cederberg Helms, Bjorn Olav Hald, Yiqiu Dong, Jonas Christoffer Fordsmann, Reena Murmu Nielsen, Jingshi Hu, Micael Lonstrup, Birger Brodin, Martin Johannes Lauritzen
Summary: The study demonstrates that precapillary sphincters and first-order capillaries are more responsive to factors such as synaptic activity, vasoactive molecules, etc., compared to surrounding arterioles and downstream capillaries, with similar kinetics. The role of precapillary sphincters and pericytes on first-order capillaries in regulating capillary blood flow and pressure is crucial in responding to brain-derived vascular signals.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Yidan Sun, Luwen Xu, Yin Li, Jian Lin, Haizhou Li, Yashan Gao, Xiaolu Huang, Hainan Zhu, Yingfan Zhang, Kunchen Wei, Yali Yang, Baojin Wu, Liang Zhang, Qingfeng Li, Caiyue Liu
Summary: This study investigated the changes in human epidermis during long-term tissue expansion therapy using single-cell RNA sequencing. The results showed that the expanding human skin epidermis maintained a similar cellular composition and lineage trajectory to its non-expanding neighbor, but cellular heterogeneity differed from the early response to expansion. Additionally, a decrease in proliferative cells and profound transcriptional changes in epidermal stem cells were detected.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Han Su, Aubrey C. Cantrell, Heng Zeng, Shai-Hong Zhu, Jian-Xiong Chen
Summary: Pericytes, as mural cells covering microvascular capillaries, play essential roles in vascular remodeling and maintenance, interact with other cells via secretome mechanisms, and have significant impacts on vasculature formation, stabilization, and regeneration. They also function as mesenchymal cells or progenitor cells in cardiovascular regeneration, contributing to vascular remodeling in various ways.
Article
Biochemistry & Molecular Biology
Eva Jover, Marco Fagnano, William Cathery, Sadie Slater, Emanuela Pisanu, Yue Gu, Elisa Avolio, Domenico Bruno, Daniel Baz-Lopez, Ashton Faulkner, Michele Carrabba, Gianni Angelini, Paolo Madeddu
Summary: This study demonstrates that human adventitial pericytes (APCs) exhibit resistance to calcification compared to bone marrow-derived mesenchymal stromal cells (BM-MSCs) and convey the anti-calcific phenotype to heart valves through miR-132. These findings suggest that APCs may be a promising cell source for cellularization of prosthetic heart valves.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Cell Biology
Jun Zhou, Erica Valentini, Michael Boutros
Summary: Tissue homeostasis is disrupted during tumorigenesis, where tumor cells can outcompete nearby normal cells by influencing cell adhesion and contractility. Tumor growth depends on the activation of the JNK/PGRP-LA/Relish signaling axis to induce death of neighboring normal cells.
DEVELOPMENTAL CELL
(2021)
Article
Materials Science, Biomaterials
Li Xiong, Chen Zhou, Lei Tong, Xiaowen Han, Yaping Zou, Zuqin Dong, Jie Liang, Yafang Chen, Yujiang Fan
Summary: Designing a smart hydrogel using rhCol III-CS for wound healing is highly desirable. The hydrogel exhibited rapid gelation at wound sites and complete coverage of irregular wounds. It also promoted cell proliferation and migration, and showed potent antibacterial properties against S. aureus and E. coli. Furthermore, the hydrogel increased collagen deposition and accelerated full-thickness wound healing, making it a promising dressing for skin wound repair and regeneration without additional drugs or cells.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Biochemistry & Molecular Biology
Mairobi Persinal-Medina, Sara Llames, Manuel Chacon, Natalia Vazquez, Marta Pevida, Ignacio Alcalde, Sergio Alonso-Alonso, Laura Maria Martinez-Lopez, Jesus Merayo-Lloves, Alvaro Meana
Summary: This study developed a polymerizable skin hydrogel consisting of keratinocytes and fibroblast within a fibrin scaffold. The results showed that this hydrogel can effectively regenerate skin wounds, shortening the time needed for transplantation compared to other skin models. It is suggested that this polymerizable skin hydrogel is an inexpensive, easy and rapid treatment for improving the treatment of skin wounds.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Immunology
Tatsuya Hasegawa, Tomonori Oka, Shadmehr Demehri
Summary: The skin serves as the primary interface between the body and the environment. The skin immune system plays a crucial role in defending against microbial pathogens and environmental insults. Type 2 alarmin cytokines are important regulators of skin immune responses, and their dysregulation is associated with allergic inflammation.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Polymer Science
Mohammad Reza Aghazadeh, Sheyda Delfanian, Pouria Aghakhani, Shahin Homaeigohar, Atefeh Alipour, Hosein Shahsavarani
Summary: Natural cellulose, obtained from bacterial and plant-based sources, shows potential as a biomaterial for tissue engineering by mimicking the extracellular matrix. Current research progress suggests that while meeting clinical application criteria is still a challenge, it is not an unachievable goal.
Article
Chemistry, Multidisciplinary
Xiaoyu Yin, Lintao Wang, Yiming Niu, Daping Xie, Qingwen Zhang, Jian Xiao, Lei Dong, Chunming Wang
Summary: This study discovered an oligosaccharide biomaterial (OG6) that stimulates specific expression of a chemokine in macrophages, leading to hair regeneration without inducing excessive inflammation. This finding opens up new possibilities for designing glycan-based therapeutic tools.
ADVANCED MATERIALS
(2023)
Review
Biotechnology & Applied Microbiology
Qiao Ruan, Shenglong Tan, Li Guo, Dandan Ma, Jun Wen
Summary: Prevascularization techniques have been proposed to solve the challenge of rapid vascularization in pulp regeneration. These techniques involve coculturing endothelial cells and pericytes to induce intercellular communication, and then introducing them into a customized artificial vascular bed or inducing self-assembly. However, there are still unresolved problems regarding cell sources, intercellular communication, and the construction of prevascularization systems for pulp regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Cell Biology
Zhaocheng Zhang, Kristy A. Warner, Andrea Mantesso, Jacques E. Nor
Summary: Dental pulp stem cells (DPSC) can differentiate into functional blood vessels via vasculogenesis, and the maturation of these vessels is regulated by PDGF-BB signaling through PDGFR-beta.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Pathology
Owen W. J. Prall, Violeta Nastevski, Huiling Xu, Christopher R. E. McEvoy, Joep H. A. Vissers, David J. Byrne, Elena Takano, Satwica Yerneni, Sarah Ellis, Thomas Green, Catherine A. Mitchell, William K. Murray, Clare L. Scott, Sean M. Grimmond, Oliver Hofmann, Anthony Papenfuss, Damien Kee, Andrew Fellowes, Ian S. Brown, Gregory Miller, M. Priyanthi Kumarasinghe, Aurel Perren, Christopher B. Nahm, Anubhav Mittal, Jaswinder Samra, Mahsa Ahadi, Stephen B. Fox, Angela Chou, Anthony J. Gill
Article
Multidisciplinary Sciences
Elaine Sanij, Katherine M. Hannan, Jiachen Xuan, Shunfei Yan, Jessica E. Ahern, Anna S. Trigos, Natalie Brajanovski, Jinbae Son, Keefe T. Chan, Olga Kondrashova, Elizabeth Lieschke, Matthew J. Wakefield, Daniel Frank, Sarah Ellis, Carleen Cullinane, Jian Kang, Gretchen Poortinga, Purba Nag, Andrew J. Deans, Kum Kum Khanna, Linda Mileshkin, Grant A. McArthur, John Soong, Els M. J. J. Berns, Ross D. Hannan, Clare L. Scott, Karen E. Sheppard, Richard B. Pearson
NATURE COMMUNICATIONS
(2020)
Article
Microbiology
Aya C. Taki, Robert Brkljaca, Tao Wang, Anson Koehler, Guangxu Ma, Jill Danne, Sarah Ellis, Andreas Hofmann, Bill C. H. Chang, Abdul Jabbar, Sylvia Urban, Robin B. Gasser
Correction
Biochemistry & Molecular Biology
M. Burgess, Y. C. E. Chen, S. Mapp, A. Blumenthal, P. Mollee, D. Gill, N. A. Saunders
Summary: Correction to the paper has been published.
Article
Dermatology
Rehan Villani, Valentine Murigneux, Josue Alexis, Seen-Ling Sim, Michael Wagels, Nicholas Saunders, H. Peter Soyer, Laurent Parmentier, Sergey Nikolaev, J. Lynn Fink, Edwige Roy, Kiarash Khosrotehrani
Summary: Infiltrative BCCs do not differ significantly in terms of genomic alterations, but show a specific type of interaction with the extracellular matrix environment regulating Wnt signaling.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Farzaneh Atashrazm, Sarah Ellis
Summary: Polarity protein PARD3 plays a crucial role in cell polarity arrangement and cancer, regulating cell arrangement by forming large complexes and influencing cancer development by binding signaling molecules. In tumors, PARD3 may act as both a tumor suppressor and a promoter, with its function dependent on physiological and cellular context.
Article
Multidisciplinary Sciences
Shira Landau, Abigail Newman, Shlomit Edri, Inbal Michael, Shahar Ben-Shaul, Yulia Shandalov, Tom Ben-Arye, Pritinder Kaur, Ming H. Zheng, Shulamit Levenberg
Summary: Lymphatic system plays a critical role in various biological processes, yet lymphangiogenesis is less studied compared to angiogenesis. By culturing human lymphatic endothelial cells on thick collagen sheets with supporting cells and blood endothelial cells, a functional lymphatic vessel network was successfully developed in a short period of time. The model provides a potential platform for investigating lymphangiogenesis and lymphatic disease mechanisms.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Sangeeta Ghuwalewala, Seon A. Lee, Kevin Jiang, Joydeep Baidya, Gopal Chovatiya, Pritinder Kaur, David Shalloway, Tudorita Tumbar
Summary: This study elucidates the molecular and cellular states of basal interfollicular epidermis (IFE) domains and their role in skin adaptation to environmental exposure. The research uncovers two paths of basal cell differentiation and identifies a novel transcription factor, Sox6, that plays an important role in IFE proliferation and survival.
Article
Allergy
Gohar Shaghayegh, Clare Cooksley, George Spyro Bouras, Beula Subashini Panchatcharam, Rejhan Idrizi, Metta Jana, Sarah Ellis, Alkis James Psaltis, Peter -John Wormald, Sarah Vreugde
Summary: This study aimed to investigate the characteristics of Staphylococcus aureus biofilms in patients with chronic rhinosinusitis (CRS) and their correlation with inflammation and disease severity. The results showed that CRS patients with nasal polyps had thicker S aureus biofilms with higher bacterial counts and higher exoprotein production compared to the control group. The numbers of inflammatory cells and disease severity were positively correlated with S aureus biofilm properties.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Wil Gardner, David A. Winkler, Ruqaya Maliki, Suzanne M. Cutts, Sarah Ellis, Robin L. Anderson, Benjamin W. Muir, Paul J. Pigram
Summary: This study applies a convolutional neural network (CNN) fusion method to fuse ToF-SIMS hyperspectral data sets, achieving resolution-enhanced data with high spatial and mass resolution. The method is applied to ToF-SIMS images of a gold mesh sample and a tumor tissue section, and the improvement is compared to another linear fusion method used in the broader MSI community.
ADVANCED MATERIALS INTERFACES
(2022)
Article
Rheumatology
Ai Li Yeo, Rangi Kandane-Rathnayake, Rachel Koelmeyer, Vera Golder, Worawit Louthrenoo, Yi-Hsing Chen, Jiacai Cho, Aisha Lateef, Laniyati Hamijoyo, Shue-Fen Luo, Yeong-Jian J. Wu, Sandra Navarra, Leonid Zamora, Zhanguo Li, Yuan An, Sargunan Sockalingam, Yasuhiro Katsumata, Masayoshi Harigai, Yanjie Hao, Zhuoli Zhang, B. M. D. B. Basnayake, Madelynn Chan, Jun Kikuchi, Tsutomu Takeuchi, Sang-Cheol Bae, Shereen Oon, Sean O'Neill, Fiona Goldblatt, Kristine (Pek Ling) Ng, Annie Law, Nicola Tugnet, Sunil Kumar, Cherica Tee, Michael Tee, Naoaki Ohkubo, Yoshiya Tanaka, Chak Sing Lau, Mandana Nikpour, Alberta Hoi, Michelle Leech, Eric F. Morand
Summary: In this study, the usefulness of serial anti-dsDNA testing in predicting flare in SLE patients who are persistently anti-dsDNA positive was investigated. The results showed that both the absolute value and change in anti-dsDNA levels predicted flares in patients with persistent anti-dsDNA positivity.
Article
Multidisciplinary Sciences
Shen Y. Y. Heazlewood, Tanveer Ahmad, Benjamin Cao, Huimin Cao, Melanie Domingues, Xuan Sun, Chad K. K. Heazlewood, Songhui Li, Brenda Williams, Madeline Fulton, Jacinta F. F. White, Tom Nebl, Christian M. M. Nefzger, Jose M. M. Polo, Benjamin T. T. Kile, Felix Kraus, Michael T. T. Ryan, Yu B. B. Sun, Peter F. M. Choong, Sarah L. L. Ellis, Minna-Liisa Anko, Susan K. K. Nilsson
Summary: Different sub-populations of megakaryocytes play important roles in generating platelets and regulating blood stem cells, and they are conserved in humans.
NATURE COMMUNICATIONS
(2023)
Article
Hematology
Shen Y. Heazlewood, Tanveer Ahmad, Monika Mohenska, Belinda B. Guo, Pradnya Gangatirkar, Emma C. Josefsson, Sarah L. Ellis, Madara Ratnadiwakara, Huimin Cao, Benjamin Cao, Chad K. Heazlewood, Brenda Williams, Madeline Fulton, Jacinta F. White, Mirana Ramialison, Susan K. Nilsson, Minna-Liisa Anko
Summary: RNA processing is critical for the development and function of megakaryocytes and platelets, and serine-arginine-rich splicing factor 3 (SRSF3) plays an essential role in megakaryocyte maturation and platelet production.
Article
Oncology
Shunfei Yan, Jiachen Xuan, Natalie Brajanovski, Madeleine R. C. Tancock, Piyush B. Madhamshettiwar, Kaylene J. Simpson, Sarah Ellis, Jian Kang, Carleen Cullinane, Karen E. Sheppard, Katherine M. Hannan, Ross D. Hannan, Elaine Sanij, Richard B. Pearson, Keefe T. Chan
Summary: In this study, TOP1 inhibition was found to enhance the therapeutic effect of CX-5461 in targeting HR-proficient HGSC by inducing a nucleolar DNA damage response and global replication stress, ultimately reducing clonogenic survival and tumor growth.
BRITISH JOURNAL OF CANCER
(2021)
Meeting Abstract
Oncology
Elaine Sanij, Katherine Hannan, Jiachen Xuan, Shunfei Yan, Jessica A. Ahern, Anna S. Trigos, Natalie Brajanovski, Jinbae Son, Keefe T. Chan, Olga Kondrashova, Elizabeth Lieschke, Matthew J. Wakefield, Sarah Ellis, Carleen Cullinane, Gretchen Poortinga, Kum Kum Khanna, Linda Mileshkin, Grant A. McArthur, John Soong, Els M. Berns, Ross D. Hannan, Clare L. Scott, Karen E. Sheppard, Richard B. Pearson
CLINICAL CANCER RESEARCH
(2020)