Article
Cell Biology
Chulpan R. Gafurova, Andrei N. Tsentsevitsky, Alexey M. Petrov
Summary: The study revealed that different types of synaptic vesicles (SVs) in nerve terminals exhibit distinct behaviors in response to low- and high-frequency stimulations. At least two SV pools were found to operate during prolonged stimulation, one for maintaining release at different frequencies and the other for responding to increasing activity.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Abhijith Radhakrishnan, Xia Li, Kirill Grushin, Shyam S. Krishnakumar, Jun Liu, James E. Rothman
Summary: The controlled release of neurotransmitters stored in synaptic vesicles is essential for information processing in the brain, relying on the fusion of vesicles with presynaptic Ca2+ influx. Through cryoelectron tomography analysis, it was found that vesicles are initially tethered to the plasma membrane by protein densities under native conditions. During the process of priming for release, a highly cooperative framework is formed between the primed vesicles and the membrane, facilitating rapid neurotransmitter release following Ca2+ influx.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Beyenech Binotti, Momchil Ninov, Andreia P. Cepeda, Marcelo Ganzella, Ulf Matti, Dietmar Riedel, Henning Urlaub, Sivakumar Sambandan, Reinhard Jahn
Summary: The study shows that ATG9-containing vesicles are enriched in synapses and resemble synaptic vesicles in size and density. These vesicles represent a distinct population with limited overlap with synaptic vesicles and other membranes of the secretory pathway, revealing surprising heterogeneity in their membrane composition. ATG9-containing vesicles may function as lipid shuttles that scavenge membrane lipids from various intracellular membranes to support autophagosome biogenesis.
Article
Neurosciences
Soulmee Koh, Wongyoung Lee, Sang Myun Park, Sung Hyun Kim
Summary: The study demonstrates the important role of Cav1 in synaptic vesicle exocytosis, with its knockdown significantly impairing synaptic vesicle function. Neurons rescued by triple mutants lacking palmitoylation sites of Cav1 show impairments in both synaptic transmission and retrieval, highlighting the importance of Cav1 palmitoylation in activity-driven synaptic vesicle dynamics.
Article
Biochemistry & Molecular Biology
Carolina Montenegro-Venegas, Debarpan Guhathakurta, Eneko Pina-Fernandez, Maria Andres-Alonso, Florian Plattner, Eckart D. Gundelfinger, Anna Fejtova
Summary: This study reveals that the protein bassoon organizes the release of neurotransmitters by regulating synaptic phosphorylation and cAMP homeostasis. Synapses lacking bassoon exhibit decreased release competence of synaptic vesicles and increased resting pool of vesicles. Furthermore, the CDK5/PDE4/cAMP signaling pathway plays a crucial role in the regulation of neurotransmitter release.
Article
Neurosciences
Emily Church, Edaeni Hamid, Zack Zurawski, Mariana Potcoava, Eden Flores-Barrera, Adriana Caballero, Kuei Y. Tseng, Simon Alford
Summary: Presynaptic inhibition plays a crucial role in presynaptic terminals, with GABAB receptors inhibiting Ca2+ entry and 5-HT1B receptors targeting SNARE complexes. The colocalization of these receptors provides synaptic integration and allows for complex presynaptic integration.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Kim Ann Saal, Carmina Warth Perez Arias, Anna-Elisa Roser, Jan Christoph Koch, Mathias Bahr, Silvio O. Rizzoli, Paul Lingor
Summary: The study showed that chronic fasudil treatment does not alter basic synaptic function or the amount of synaptic proteins, but does change the dynamics of synaptic vesicles. This leads to a reduction in the synaptic response upon stimulation.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Neurosciences
Shankar Ramachandran, Shelagh Rodgriguez, Mariana Potcoava, Simon Alford
Summary: This study investigates the number, subtypes, and locations of Ca2+ channels involved in neurotransmitter release at central synapses. By using single channel analysis and lattice light-sheet microscopy, the researchers discovered that a small pool of Ca2+ channels, comprising N-, P/Q-, and R-subtypes, are available to gate neurotransmitter release. They also found that as few as one channel may trigger neurotransmitter release. These findings provide important insights into the understanding of Ca2+ channel and synaptic dysfunction.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Cell Biology
Bo Shi, Ying-Hui Jin, Ling-Gang Wu
Summary: This study investigates the role of dynamin 1 in endocytosis and vesicle size regulation. The results show that knockout of dynamin 1 slows down endocytosis and increases the diameter of synaptic vesicles, suggesting an important function of dynamin 1 in controlling vesicle size during pit formation.
Article
Biochemistry & Molecular Biology
Sung Rae Kim, Yunkyung Eom, Sung Hoon Lee
Summary: This study investigated the differences in presynaptic terminals of cultured hippocampal neurons derived from male and female mice. The findings revealed that female neurons had a higher number of synaptic vesicles compared to male neurons.
NEUROCHEMISTRY INTERNATIONAL
(2023)
Article
Neurosciences
Chen Xu, Sidong Chen, Xingxiang Chen, Ka Hei Ho, Chungwon Park, Hanna Yoo, Suk-Ho Lee, Hyokeun Park
Summary: Huntington's disease (HD) is a neurodegenerative disease caused by a genetic mutation, leading to the degeneration of striatum. The mechanisms underlying the neurodegeneration in HD are not well understood, but abnormal synaptic transmission is suggested to be an early sign. This study explores how the mutant huntingtin protein affects synaptic vesicle exocytosis in HD striatal neurons.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2023)
Review
Neurosciences
Kate L. Prichard, Nicholas S. O'Brien, Sari R. Murcia, Jennifer R. Baker, Adam McCluskey
Summary: Endocytosis is a crucial process for cell health and signaling, specifically clathrin mediated endocytosis (CME) plays a key role in synaptic vesicle recycling and intracellular communication in the brain. This review focuses on clathrin and dynamin, two proteins involved in CME, and discusses the potential therapeutic interventions and inhibitors for conditions involving defects in CME.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Hang Xing, Xiong Han, Sihan Xu, Zhongyu Sun, Shijun Yang
Summary: This study demonstrates the therapeutic effects of Brivaracetam (BRV) in altering short-term synaptic activities and abnormal low-frequency brain activities during interictal periods of epileptic seizures. BRV slows down the mobilization of synaptic vesicles and reduces short-term synaptic potentiation (STP), contributing to the treatment of epileptic seizures. These findings advance our understanding of BRV's pharmacological role and optimization of treatment strategies.
JOURNAL OF MOLECULAR NEUROSCIENCE
(2022)
Review
Neurosciences
Hassaam Choudhry, Meha Aggarwal, Ping-Yue Pan
Summary: Synj1, a phosphoinositide phosphatase, plays a critical role in neurological disorders, particularly in synaptic membrane trafficking and endosomal trafficking. Mutations and variants of Synj1 associated with synaptic vesicle recycling are linked to neurodegenerative diseases. Despite advancements in understanding Synj1's structure and function, the mechanism by which it connects to disease pathogenesis remains to be fully addressed.
NEUROSCIENCE LETTERS
(2021)
Article
Anatomy & Morphology
Kohgaku Eguchi, Jacqueline Montanaro, Elodie Le Monnier, Ryuichi Shigemoto
Summary: This study summarizes how the morphological parameters of Ca(V)2 distribution obtained using SDS-FRL differ depending on the different types of synapses and could correspond to functional properties in synaptic transmission.
FRONTIERS IN NEUROANATOMY
(2022)
Article
Cardiac & Cardiovascular Systems
Tai-Ming Ko, Ho-Chang Kuo, Jeng-Sheng Chang, Shih-Ping Chen, Yi-Min Liu, Hui-Wen Chen, Fuu-Jen Tsai, Yi-Ching Lee, Chien-Hsiun Chen, Jer-Yuarn Wu, Yuan-Tsong Chen
CIRCULATION RESEARCH
(2015)
Article
Biochemistry & Molecular Biology
Yu-Wei Syu, Hao-Wen Lai, Chung-Lin Jiang, Hong-Yuan Tsai, Chung-Chih Lin, Yi-Ching Lee
HUMAN MOLECULAR GENETICS
(2018)
Article
Cell Biology
Yuh-Charn Lin, Yi-Ching Lee, Ling-Hui Li, Chien-Jui Cheng, Ruey-Bing Yang
JOURNAL OF CELL SCIENCE
(2014)
Article
Medicine, General & Internal
C. -H. Chen, C. -S. Lee, M. -T. M. Lee, W. -C. Ouyang, Chiao-Chicy Chen, M. -Y. Chong, J. -Y. Wu, H. K. -L. Tan, Y. -C. Lee, L. -J. Chuo, N. -Y. Chiu, H. -Y. Tsang, T. -J. Chang, F. -W. Lung, C. -H. Chiu, C. -H. Chang, Y. -S. Chen, Y. -M. Hou, Cheng-Chung Chen, T. -J. Lai, C. -L. Tung, C. -Y. Chen, H. -Y. Lane, T. -P. Su, J. Feng, J. -J. Lin, C. -J. Chang, P. -R. Teng, C. -Y. Liu, C. -K. Chen, I. -C. Liu, J. -J. Chen, T. Lu, C. -C. Fan, C. -K. Wu, C. -F. Li, K. H. -T. Wang, L. S. -H. Wu, H. -L. Peng, C. -P. Chang, L. -S. Lu, Y. -T. Chen, A. T. -A. Cheng
NEW ENGLAND JOURNAL OF MEDICINE
(2014)
Article
Multidisciplinary Sciences
Chia-Jung Chang, Ho-Chang Kuo, Jeng-Sheng Chang, Jong-Keuk Lee, Fuu-Jen Tsai, Chiea Chuen Khor, Li-Ching Chang, Shih-Ping Chen, Tai-Ming Ko, Yi-Min Liu, Ying-Ju Chen, Young Mi Hong, Gi Young Jang, Martin L. Hibberd, Taco Kuijpers, David Burgner, Michael Levin, Jane C. Burns, Sonia Davila, Yuan-Tsong Chen, Chien-Hsiun Chen, Jer-Yuarn Wu, Yi-Ching Lee
Article
Multidisciplinary Sciences
Yi-Ching Lee, I-Wen Song, Ya-Ju Pai, Sheng-De Chen, Yuan-Tsong Chen
SCIENTIFIC REPORTS
(2017)
Article
Multidisciplinary Sciences
Govinda Lenka, Mong-Hsun Tsai, Hsin-Chieh Lin, Jen-Hao Hsiao, Yi-Ching Lee, Tzu-Pin Lu, Jang-Ming Lee, Chung-Ping Hsu, Liang-Chuan Lai, Eric Y. Chuang
SCIENTIFIC REPORTS
(2017)
Article
Multidisciplinary Sciences
Tai-Ming Ko, Jeng-Sheng Chang, Shih-Ping Chen, Yi-Min Liu, Chia-Jung Chang, Fuu-Jen Tsai, Yi-Ching Lee, Chien-Hsiun Chen, Yuan-Tsong Chen, Jer-Yuarn Wu
SCIENTIFIC REPORTS
(2019)
Article
Neurosciences
Wei-Ping Jen, Hui-Mei Chen, Yow-Sien Lin, Yijuang Chern, Yi-Ching Lee
MOLECULAR NEUROBIOLOGY
(2020)
Article
Genetics & Heredity
Chung-Lin Jiang, Wei-Ping Jen, Chang-Yu Tsao, Li-Ching Chang, Chien-Hsiun Chen, Yi-Ching Lee
Article
Genetics & Heredity
Todd A. Johnson, Yoichi Mashimo, Jer-Yuarn Wu, Dankyu Yoon, Akira Hata, Michiaki Kubo, Atsushi Takahashi, Tatsuhiko Tsunoda, Kouichi Ozaki, Toshihiro Tanaka, Kaoru Ito, Hiroyuki Suzuki, Hiromichi Hamada, Tohru Kobayashi, Toshiro Hara, Chien-Hsiun Chen, Yi-Ching Lee, Yi-Min Liu, Li-Ching Chang, Chun-Ping Chang, Young-Mi Hong, Gi-Young Jang, Sin-Weon Yun, Jeong-Jin Yu, Kyung-Yil Lee, Jae-Jung Kim, Taesung Park, Jong-Keuk Lee, Yuan-Tsong Chen, Yoshihiro Onouchi
Summary: The study identified a significant association between SNVs in the IGHV cluster and susceptibility to Kawasaki disease (KD). Furthermore, they observed highly skewed allelic usage in relation to a specific gene in the IGHV cluster, suggesting potential B-cell related mechanisms in the pathogenesis of KD. This association may shed light on common mechanisms between KD, acute rheumatic fever (ARF), and rheumatic heart disease (RHD).
JOURNAL OF HUMAN GENETICS
(2021)
Article
Medicine, Research & Experimental
Brandon M. Wagner, Jerid W. Robinson, Yun-Wen Lin, Yi-Ching Lee, Nabil Kaci, Laurence Legeai-Mallet, Lincoln R. Potter
Summary: Preventing GC-B dephosphorylation rescues reduced skeletal growth in a mouse model of achondroplasia.
Article
Developmental Biology
Tzu-Lun Tseng, Ying-Ting Wang, Chang-Yu Tsao, Yi-Teng Ke, Yi-Ching Lee, Hwei-Jan Hsu, Kenneth D. Poss, Chen-Hui Chen
Summary: Research identified a gene called pan in zebrafish that can suspend juvenile growth but resume a robust growth trajectory after release from restriction, with the mechanism being conserved in flies, zebrafish, and mice. This suggests that safe, reversible pauses of juvenile growth can be mediated by targeting the activity of a single gene.
Article
Genetics & Heredity
Chung-Lin Jiang, Chang-Yu Tsao, Yi-Ching Lee
Summary: In this study, researchers found that vitamin C has greater beneficial effects on metabolism in a specific genetically susceptible mouse model fed a high-fat diet. Vitamin C supplementation improved the sensitivity of the mice to high-fat diet-induced metabolic dysfunction and reduced inflammation and weight gain.
GENES AND NUTRITION
(2022)
Article
Medicine, Research & Experimental
Yun-Wen Lin, Hsiao-Jung Kao, Wei-Ting Chen, Cheng-Fu Kao, Jer-Yuarn Wu, Yuan-Tsong Chen, Yi-Ching Lee
Summary: This study established a monitoring system for FGFR3 activation and identified a plant extract and two bioactive porphyrins that can suppress FGFR3 signaling. Further research showed that these compounds can reduce excessive FGFR3 activity and improve skeletal abnormalities.