期刊
JOURNAL OF CLINICAL IMMUNOLOGY
卷 30, 期 2, 页码 253-259出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10875-009-9353-1
关键词
Immune thrombocytopenia; interleukin-21 T helper 1; T helper 17
类别
资金
- National Natural Science Foundation [30600680, 30471941, 30770922, 30470742, 30570779, 30600259, 30628015, 30300312]
- 973 Project [2006CB503800]
- Clinical Research Project of Chinese Ministry of Health [2007-2009]
- Research Project of National Public Fare [200802031]
- Cultivation Fund of the Key Scientific and Technical Innovation Project, Ministry of Education of China [NO704030]
- Shandong Technological Development Project [BS2009SW014, 2005BS03022, 2005GG4202018]
- Taishan Scholar Foundation
- SRF
Interleukin-21 (IL-21) is critical in the development of autoimmune diseases. The role of IL-21 in the pathogenesis of immune thrombocytopenia (ITP) remains unknown. We examined the expression of IL-21, IL-17, and interferon (IFN)-gamma in ITP patients and controls by enzyme-linked immunosorbent assay and flow cytometry. Detection of specific anti-platelet GPIIb/IIIa and/or GPIb/IX autoantibodies was measured by modified monoclonal antibody specific immobilization of platelet antigens. IL-21 was expressed on both CD3(+)CD8(-) T cells and CD3(+)CD8(+) T cells by flow cytometry. Plasma IL-21 level and the percentage of CD3(+)CD8(-)IL-21(+) T cells and CD3(+)CD8(+)IL-21(+) T cells were significantly elevated in ITP patients compared to controls. The percentage of CD3(+)CD8(-)IL-17(+) T (Th17), CD3(+)CD8(-)IFN-gamma(+) T (Th1), and CD3(+)CD8(+)IFN-gamma(+) T (Tc1) cells also significantly increased in ITP patients. Moreover, we found a significant positive correlation between CD3(+)CD8(-)IL-21(+) T cells and Th17 cells. In addition, a positive correlation between CD3(+)CD8(-)IL-21(+) T cells and Th1 cells was also found. Together, our results indicated a possible role of IL-21 in ITP patients correlated to Th17 and Th1 cells, and blockade of IL-21 may be a reasonable therapeutic strategy for ITP especially those with active disease.
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