Article
Immunology
Dylan Krajewski, Debayon Paul, Shujun Ge, Evan Jellison, Joel S. Pachter
Summary: In experimental autoimmune encephalomyelitis (EAE), claudin-5 (CLN-5) was found on leukocytes in the blood and central nervous system (CNS), potentially facilitating leukocyte transendothelial migration (TEM) across the blood-brain barrier. Flow cytometry analysis showed CLN-5(+) populations among various leukocyte subtypes, with higher levels in CNS tissue than in blood, suggesting preferential access to the CNS. This novel mechanism may guide leukocytes to sites for diapedesis across the BBB.
JOURNAL OF NEUROINFLAMMATION
(2021)
Article
Chemistry, Multidisciplinary
Badrul Alam Bony, Aria W. Tarudji, Hunter A. Miller, Saiprasad Gowrikumar, Sourav Roy, Evan T. Curtis, Connor C. Gee, Alex Vecchio, Punita Dhawan, Forrest M. Kievit
Summary: Aging-induced alterations to the blood-brain barrier are increasingly recognized as a primary event in chronic neurological disorders; development of nanoparticles targeting regions of increased claudin-1 expression shows promise for treating these diseases; claudin-1 plays a role in reducing BBB integrity during aging, and nanoparticles can be used to actively target altered claudin-1 expression for therapeutic delivery.
Article
Chemistry, Multidisciplinary
Valentina Castagnola, Lieselot Deleye, Alice Podesta, Edra Jaho, Fabrizio Loiacono, Doriana Debellis, Martina Trevisani, Dinu Zinovie Ciobanu, Andrea Armirotti, Francesco Pisani, Emmanuel Flahaut, Ester Vazquez, Mattia Bramini, Fabrizia Cesca, Fabio Benfenati
Summary: Due to their biocompatibility and high cargo capability, graphene-based materials are considered as an ideal brain delivery system. In this study, we used two in vitro models to investigate the interactions between graphene oxide and few-layer graphene with the blood-brain barrier. Our results show that the two graphene materials have good biocompatibility and do not significantly affect the integrity and functionality of the barrier, but their translocation across the barrier is rare.
Review
Cell Biology
Ece Bayir, Aylin Sendemir
Summary: The blood-brain barrier is a highly selective cellular monolayer unique to the central nervous system microvasculature, mediating communication with the body by regulating the passage of molecules. Recent studies have highlighted the importance of intermediate filaments in the formation and function of cell-cell junctions, particularly vimentin in endothelial cells. Intermediate filaments may have been overlooked as key targets in regulating blood-brain barrier permeability in health and disease.
Article
Endocrinology & Metabolism
Lars Winkler, Rosel Blasig, Olga Breitkreuz-Korff, Philipp Berndt, Sophie Dithmer, Hans C. Helms, Dmytro Puchkov, Kavi Devraj, Mehmet Kaya, Zhihai Qin, Stefan Liebner, Hartwig Wolburg, Anuska Andjelkovic, Andre Rex, Ingolf E. Blasig, Reiner F. Haseloff
Summary: The study shows that Cldn3 and occludin play a protective role in the stroke by maintaining the integrity of TJ and BBB, but also contribute to the development of edema and infarction.
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2021)
Article
Cell Biology
Daniel Maucher, Birte Schmidt, Julia Schumann
Summary: Dysfunction of the endothelial barrier is central in inflammatory processes like sepsis and atherosclerosis. In this study, cytokine-induced weakening of the endothelial barrier was associated with changes in the transcriptome and upregulation of miR-29a-3p, miR-29b-3p, and miR-155-5p, which target central adhesion proteins' mRNAs. This suggests a posttranscriptional mechanism regulated by inflammatory cytokines.
Article
Cell Biology
Erik Axel Andersson, Eridan Rocha-Ferreira, Henrik Hagberg, Carina Mallard, Carl Joakim Ek
Summary: The study investigated the impact of GMH on the BBB using a rat model, showing that GMH causes temporary dysfunction in the BBB that normalizes within 5 days. Tight-junction proteins were identified as potential biomarkers for GMH.
Article
Biochemistry & Molecular Biology
Qian Yue, Xinhua Zhou, Zaijun Zhang, Maggie Pui Man Hoi
Summary: The study found that murine sA beta (1-42) oligomers had moderate toxicity on an endothelial and astrocyte co-culture, leading to endothelial barrier disruptions induced by deleterious soluble factors released from astrocytes which disrupted endothelial VEGFR2 signaling and perturbed cell survival and barrier stabilization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Endocrinology & Metabolism
Markus Gindorf, Steffen E. Storck, Anke Ohler, Franka Scharfenberg, Christoph Becker-Pauly, Claus U. Pietrzik
Summary: Mep1b is a novel regulator of tight junction composition and blood-brain barrier integrity in brain endothelium. Knock-out of Mep1b in mice showed increased expression of TJ proteins and improved TEER, suggesting Mep1b plays a role in BBB tightness regulation.
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2021)
Article
Plant Sciences
Bowen Liu, Yiyang Li, Yan Han, Shengpeng Wang, Hua Yang, Yonghua Zhao, Ping Li, Yitao Wang
Summary: Notoginsenoside R1 treatment significantly reduced BBB leakage and cerebral infarction volume, weakened neurological deficits in post-stroke rats. Moreover, it inhibited the activity of MMPs in infarcted cortex and striatum, down-regulated MMP2, 9 and up-regulated zo1 and claudin5 expressions in penumbra. In vitro, Notoginsenoside R1 treatment decreased OGD-induced endothelial barrier permeability, restored expressions of zo1, claudin5 on cellular membrane and cytoplasm, as well as mediated membrane redistribution of occludin and caveolin1 from actin cytoskeletal fraction.
Review
Neurosciences
Xiaoshu Chen, Xinru Pang, Abrey J. Yeo, Siwen Xie, Mengting Xiang, Bin Shi, Gongchang Yu, Chao Li
Summary: The blood-brain barrier is an important protective barrier of the central nervous system that prevents harmful substances from entering. Dysfunction of the blood-brain barrier is an early sign of nervous system diseases. Recent studies have focused on ferroptosis, a novel cell death mode caused by iron-mediated lipid peroxidation accumulation, and its potential role in blood-brain barrier dysfunction. Understanding the relationship between ferroptosis and blood-brain barrier dysfunction may lead to new treatment targets for brain diseases.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Neurosciences
Shanshan Zhang, Lin Gan, Fengye Cao, Hao Wang, Peng Gong, Congcong Ma, Li Ren, Yubo Lin, Xianming Lin
Summary: This review provides an overview of the three barriers formed between the cerebrovascular and the brain parenchyma, focusing on the composition and function of the blood-brain barrier (BBB). It discusses the limitations of the BBB for drug delivery and explores potential strategies for improving CNS drug delivery.
BRAIN RESEARCH BULLETIN
(2022)
Review
Food Science & Technology
Marta Justyna Koziel, Maksymilian Ziaja, Agnieszka Wanda Piastowska-Ciesielska
Summary: The intestinal barrier acts as the main defense against substances entering the body, ensuring balance in the organism; Mycotoxins, toxic fungi metabolites, may disrupt homeostasis by affecting the intestinal barrier; Claudins, proteins involved in tight junction formation between epithelial cells, play a significant role in responding to mycotoxin-induced cytotoxicity.
Article
Neurosciences
Wei Li, Jinlong Zou, Junkui Shang, Chenhao Gao, Ruihua Sun, Ruijie Liu, Huixia Cao, Yanliang Wang, Jiewen Zhang
Summary: The blood-brain barrier (BBB) is a complex interface composed of endothelial cells that plays a crucial role in maintaining the proper functioning of the neurons. The mechanism of BBB formation and maintenance remains largely unknown, but recent studies have highlighted the importance of vesicle transcytosis in maintaining the integrity of the BBB.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Karolina Kriauciunaite, Agne Pociute, Aida Kausyle, Alexei Verkhratsky, Augustas Pivoriunas
Summary: In this study, the contribution of MAPK/ERK and STAT3 signaling pathways to the inhibitory effects of exogenous bFGF was analyzed. The results showed that the MAPK/ERK signaling pathway does not affect the autocrine bFGF signaling-dependent barrier function of BECs, but is largely responsible for the disruptive effects of the exogenous bFGF. These findings suggest that bFGF may dynamically regulate the permeability of the endothelial blood-brain barrier.
NEUROCHEMICAL RESEARCH
(2023)
Article
Allergy
Aasha Harish, Stanley A. Schwartz
CLINICAL REVIEWS IN ALLERGY & IMMUNOLOGY
(2020)
Article
Immunology
Lili Dai, An Liu, Hongwei Zhang, Hao Wu, Tong Zhang, Bin Su, Ying Shao, Jianwei Li, Jiangzhu Ye, Sarah Robbins Scott, Supriya D. Mahajan, Stanley A. Schwartz, Hongwei Yu, Lijun Sun
CURRENT HIV RESEARCH
(2019)
Article
Pharmacology & Pharmacy
Anu Sharma, Anita Yadav, Katherine Cwiklinski, Elizabeth Quaye, Ravikumar Aalinkeel, Supriya D. Mahajan, Stanley A. Schwartz, Rakesh Kumar Sharma
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2019)
Letter
Neurosciences
Qing Ma, Giovanni Schifitto, Charles Venuto, Andrew Ocque, Stephen Dewhurst, Gene D. Morse, Ravikumar Aalinkeel, Stanley A. Schwartz, Supriya D. Mahajan
JOURNAL OF NEUROIMMUNE PHARMACOLOGY
(2020)
Correction
Allergy
Aasha Harish, Stanley A. Schwartz
CLINICAL REVIEWS IN ALLERGY & IMMUNOLOGY
(2020)
Article
Chemistry, Medicinal
Julia C. Bulmahn, Hilliard L. Kutscher, Katherine Cwiklinski, Stanley A. Schwartz, Paras N. Prasad, Ravikumar Aalinkeel
JOURNAL OF PHARMACEUTICAL SCIENCES
(2020)
Letter
Critical Care Medicine
Einas Batarseh, Brian P. Kersten, Anna C. Pinelo, Jamie N. Nadler, Stanley A. Schwartz
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2020)
Letter
Critical Care Medicine
Einas Batarseh, Brian P. Kersten, Anna C. Pinelo, Vincent Vertalino, Jamie N. Nadler, Stanley A. Schwartz
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2021)
Article
Oncology
Satish Sharma, Katherine Cwiklinski, Donald E. Sykes, Supriya D. Mahajan, Kent Chevli, Stanley A. Schwartz, Ravikumar Aalinkeel
Summary: Prostate specific membrane antigen and galectins play important roles in prostate cancer. Their expression pattern can be used as indicators for diagnosis and treatment. Combined inhibition of prostate specific membrane antigen and galectin-3 shows potential in prostate cancer treatment.
Article
Biochemistry & Molecular Biology
Khoo Ting Chean, Ravikumar Aalinkeel, Serfraz Abbasi, Anna V. Sharikova, Stanley A. Schwartz, Alexander Khmaladze, Supriya D. Mahajan
Summary: METH and HIV Tat treatment increases oxidative stress and causes DNA damage in microglia, affecting cellular metabolism. Mitochondrial dysfunction plays a key role in the neuropathology induced by METH and/or HIV Tat, while changes in the concentration of proteins, lipids, and nucleotides in microglial cells may contribute to the neuropathology associated with METH abuse in HIV patients.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Liana Bruggemann, Zackary Falls, William Mangione, Stanley A. Schwartz, Sebastiano Battaglia, Ravikumar Aalinkeel, Supriya D. Mahajan, Ram Samudrala
Summary: Pharmacogenomics is a rapidly growing field that aims to provide personalized care to every patient. The Computational Analysis of Novel Drug Opportunities (CANDO) platform was developed for therapeutic discovery, screening optimal compounds using large protein libraries. CANDO's precision medicine drug discovery pipeline predicts osimertinib to synergize with four KRAS inhibitors in treating non-small cell lung cancer (NSCLC). Validation studies confirmed the synergistic effect of osimertinib in combination with KRAS inhibitors ARS-1620 and BAY-293, and gene expression studies identified MAPK expression as strongly correlated with decreased cellular proliferation following treatment with KRAS inhibitor BAY-293, indicating the potential of the precision medicine pipeline in identifying effective compounds.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Satish Sharma, Katherine Cwiklinski, Supriya D. Mahajan, Stanley A. Schwartz, Ravikumar Aalinkeel
Summary: This study investigates the combination therapy of plant-derived natural product quercetin with the standard chemotherapy drug docetaxel in castration-resistant prostate cancer. The results show that quercetin sensitizes cancer cells to lower doses of docetaxel, reduces drug-induced toxicity, and has additive and synergistic effects for efficient cancer cell killing. The combination approach provides a novel therapeutic modality to enhance the efficacy of docetaxel in a nontoxic manner.
Review
Microbiology
Satish Sharma, James Mohler, Supriya D. Mahajan, Stanley A. Schwartz, Liana Bruggemann, Ravikumar Aalinkeel
Summary: Biofilm is a complex structure consisting of bacterial colonies residing in an exopolysaccharide matrix that attaches to foreign surfaces in a living organism. It frequently leads to nosocomial, chronic infections. Antibiotics alone are ineffective in treating biofilm infections due to antibiotic resistance developed by the bacteria within the biofilm. This review provides a concise summary of the theory behind biofilm composition, formation, and drug-resistant infections, as well as cutting-edge approaches to counteract and treat biofilm. Given the high frequency of medical device-induced infections caused by biofilm, innovative technologies are needed to manage the complexities posed by biofilm.
Article
Materials Science, Biomaterials
Anu Sharma, Abhishek Kumar, Changning Li, Puja Panwar Hazari, Supriya D. Mahajan, Ravikumar Aalinkeel, Rakesh Kumar Sharma, Mark T. Swihart
Summary: The study investigated the anticancer effect of CBD/Mg-GA MOF on rat glioma brain cancer cells, demonstrating potential therapeutic benefits through modulation of mitochondrial function and ROS generation to trigger apoptotic cascades. This MOF shows promising anti-cancer, anti-inflammatory, and anti-oxidant properties, highlighting its potential as a therapeutic option for glioblastoma.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Meeting Abstract
Critical Care Medicine
M. J. Mammen, D. E. Sykes, K. Cwiklinski, R. Aalinkeel, S. Schwartz, M. Sands, S. Mahajan
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2019)
