期刊
ACTA BIOMATERIALIA
卷 11, 期 -, 页码 68-79出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2014.09.010
关键词
Polymer brushes; Protein adsorption; Quartz crystal microbalance; X-ray photoelectron spectroscopy; Stimuli-responsive interfaces
资金
- Belgian Science Policy through the Interuniversity Attraction Pole Program [P7/05]
- FNRS
- Region Wallonne (AMOVIM project)
Adsorption of proteins on surfaces placed in biological fluids is a ubiquitous and mostly irreversible phenomenon, desirable or not, but often uncontrolled. Adsorption of most proteins on poly(ethylene oxide) (PEO) brushes is very limited, while the amount of proteins adsorbed on poly(acrylic acid) (PM) brushes varies with the pH and ionic strength (I) of the protein solution. Mixed brushes of PEO and PM are designed here to reversibly adsorb and desorb albumin, lysozyme, collagen and immunoglobulin G, four very different proteins in terms of size, solubility and isoelectric point. Protein adsorption and desorption are monitored using X-ray photoelectron spectroscopy, as well as with quartz crystal microbalance for in situ and real-time measurements. Large amounts of protein are adsorbed and then nearly completely desorbed on mixed PEO/PAA brushes by a simple pH and I trigger. The mixed brushes thus nicely combine the properties of pure PM and pure PEO brushes. These adsorption/desorption cycles are shown to be repeated with high efficiency. The high-performance smart substrates created here could find applications in domains as diverse as biosensors, drug delivery and nanotransport. (C) 2014 Acts Materialia Inc. Published by Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据