Article
Chemistry, Applied
Shima Jaldani, Elham Sarfaraz Khabbaz, Melika Jooyandeh, Reza Farhoosh
Summary: By simultaneously monitoring the concentration of LOOH and CD during the peroxidation process of different oils, valuable insights into lipid peroxidation with respect to different species of diene hydroperoxides/non-hydroperoxides can be obtained.
Review
Chemistry, Medicinal
Plamena R. Angelova, Noemi Esteras, Andrey Y. Abramov
Summary: With the aging population, neurodegenerative diseases have become a major challenge in modern society, with lipid peroxidation playing a key role in the mechanism of neuronal loss. Research suggests that products of lipid peroxidation can trigger neuronal cell death, providing potential new therapeutic directions for neurodegenerative disorders.
MEDICINAL RESEARCH REVIEWS
(2021)
Article
Biochemistry & Molecular Biology
Yang Liu, Yuan Wang, Zhi Lin, Rui Kang, Daolin Tang, Jiao Liu
Summary: SLC25A22 acts as a metabolic repressor of ferroptosis by producing glutathione and monounsaturated fatty acids, providing a previously unrecognized defense pathway against ferroptotic cell death.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Biochemistry & Molecular Biology
Natalia Belosludtseva, Vlada S. Starinets, Irina B. Mikheeva, Maxim N. Belosludtsev, Mikhail Dubinin, Galina D. Mironova, Konstantin N. Belosludtsev
Summary: Long-term hyperglycemia in diabetes mellitus can cause damage to cardiomyocytes and dysfunction in heart mitochondria. However, the use of uridine, a pyrimidine nucleoside, has been found to improve cardiac function, restore mitochondrial homeostasis, and protect against structural and functional damage to cardiac mitochondria in diabetic mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Irene Villalon-Garcia, Monica Alvarez-Cordoba, Suleva Povea-Cabello, Marta Talaveron-Rey, Marina Villanueva-Paz, Raquel Luzon-Hidalgo, Juan M. Suarez-Rivero, Alejandra Suarez-Carrillo, Manuel Munuera-Cabeza, Joaquin J. Salas, Rafael Falcon-Moya, Antonio Rodriguez-Moreno, Jose A. Armengol, Jose A. Sanchez-Alcazar
Summary: In this study, the presence of lipid peroxidation, iron accumulation and mitochondrial dysfunction in two cellular models of PLA2G6-Associated Neurodegeneration (PLAN) was examined. Vitamin E was found to reverse the pathological features in both models, suggesting its potential as a therapeutic target for the disease.
NEUROBIOLOGY OF DISEASE
(2022)
Article
Food Science & Technology
Tian-Tian Wang, Yong Yang, Feng Wang, Wen-Ge Yang, Jin-Jie Zhang, Zu-Quan Zou
Summary: Epidemiologic and preclinical studies have shown that marine n-3 polyunsaturated fatty acids exhibit promising chemoprevention against breast cancer. One specific form, Docosahexaenoic acid monoglyceride (MAG-DHA), has demonstrated significant growth inhibition in breast cancer cells through apoptosis and oxidative stress-mediated endoplasmic reticulum stress.
JOURNAL OF FOOD SCIENCE
(2021)
Article
Food Science & Technology
Elham Sarfaraz Khabbaz, Shima Jaldani, Reza Farhoosh
Summary: The kinetics of LOOH and LCO concentration changes were investigated during peroxidation of purified and unpurified sunflower oils at 60 degrees C. Two distinct peroxidation steps were observed, with significantly different rates of LOOH formation and decomposition. The first step, characterized by dominant peroxidation of linoleic acid, was identified as crucial for determining the quality of commercial sunflower oils from sensory and toxicological aspects.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kai Shan, Jiaqi Li, Qin Yang, Kang Chen, Shanshan Zhou, Lingling Jia, Guoling Fu, Yumin Qi, Qizai Wang, Yong Q. Chen
Summary: Ferroptosis caused by polyunsaturated fatty acid (PUFA) peroxidation plays a significant role in the pathogenesis of acute kidney injury (AKI), suggesting that dietary intake of PUFA may pose a risk for individuals susceptible to AKI. However, in addition to ferroptosis, other mechanisms such as inflammation-associated necroptosis and pyroptosis also contribute to different types of AKI. Therefore, further evaluation is needed to understand the role of PUFA in different AKIs, especially & omega;3 PUFA which is commonly consumed as a food supplement.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Plamena R. Angelova
Summary: Neurodegeneration encompasses over 300 neurological diseases characterized by neuronal loss and protein depositions, with factors such as mitochondrial dysfunction and oxidative stress playing key roles in their pathogenesis. Age is the main risk factor for developing neurodegenerative diseases, along with genetic predisposition and environmental toxin exposure. The accumulation of damage with age, particularly in systems regulated by free radicals, highlights the challenges in maintaining protein synthesis and degradation mechanisms.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
John O. Onukwufor, Robert T. Dirksen, Andrew P. Wojtovich
Summary: Alzheimer's disease is a neurodegenerative disease characterized by neuronal dysfunction and memory and cognitive decline. Iron dysregulation and iron-dependent cell death are associated with AD. Targeting ferroptosis, a specific form of cell death, may be a potential therapeutic intervention for AD.
Article
Biochemistry & Molecular Biology
Konstantin N. Belosludtsev, Vlada S. Starinets, Eugeny Yu Talanov, Irina B. Mikheeva, Mikhail Dubinin, Natalia Belosludtseva
Summary: Alisporivir has a positive impact on glucose utilization in diabetic mice, preventing diabetes-induced changes in mitochondrial ultrastructure and ATP synthase levels, and restoring mitochondrial function. Additionally, Alisporivir modulates mitochondrial gene expression, inhibits sensitivity to MPT pore opening, and normalizes mitochondrial dynamics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Shiqi Wu, Chao Mao, Lavanya Kondiparthi, Masha V. Poyurovsky, Kellen Olszewski, Boyi Gan
Summary: This study reveals the mechanism of GPD2 in defense against ferroptosis, suppressing mitochondrial ferroptosis by generating ubiquinol. This finding provides a new avenue for targeting ferroptosis in disease treatment.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Dominik C. Fuhrmann, Bernhard Bruene
Summary: Ferroptosis is an iron-dependent form of cell death triggered by disturbed membrane integrity caused by overproduction of lipid peroxides. It involves alterations in iron metabolism, response to oxidative stress, and lipid peroxide production. Ferroptosis contributes to tissue damage in conditions like brain and heart injury but can be desirable for overcoming chemotherapy resistance. MicroRNAs, iron homeostasis, and hypoxia play important regulatory roles in ferroptosis.
Article
Biochemistry & Molecular Biology
Yunyang Wang, Mo Wang, Yunshan Liu, Hui Tao, Somesh Banerjee, Shanthi Srinivasan, Elizabeta Nemeth, Mark J. Czaja, Peijian He
Summary: Iron excess and deficiency can cause cellular stress and death through the common mechanism of impaired autophagic function.
Article
Biochemistry & Molecular Biology
Takujiro Homma, Sho Kobayashi, Hideyo Sato, Junichi Fujii
Summary: Mitochondrial superoxide plays a key role in ferroptosis induced by cysteine starvation, with suppression of complex III superoxide production effectively protecting cells from lipid peroxidation.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Biotechnology & Applied Microbiology
Menghua Zhang, Taiki Sunaba, Yiting Sun, Takahiro Shibata, Kazunori Sasaki, Hiroko Isoda, Hideo Kigoshi, Masaki Kita
JOURNAL OF ANTIBIOTICS
(2020)
Article
Biochemistry & Molecular Biology
Miho Chikazawa, Jun Yoshitake, Sei-Young Lim, Shiori Iwata, Lumi Negishi, Takahiro Shibata, Koji Uchida
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Kana Hasegawa, Keiko Kuwata, Jun Yoshitake, Sayako Shimomura, Koji Uchida, Takahiro Shibata
Summary: Inflammatory bowel diseases lead to upregulation of proteins in extracellular vesicles, particularly an increase in expression of epidermal growth factor receptor which promotes cell proliferation. The findings suggest that inflamed colon-derived extracellular vesicles promote tumor development by activating fibroblasts.
Article
Biochemistry & Molecular Biology
Keitaro Yoshioka, Haruki Yamashita, Kazunori Shimizu, Sayako Shimomura, Takahiro Shibata, Jun-ichi Miyazaki, Hiroyuki Honda
Summary: This study identified new peptides with FFAR1 agonist activity as potential new drug candidates for treating type 2 diabetes mellitus. By utilizing phage display and physiochemical property prediction methods, peptides with high FFAR1 agonist activity can be screened. These findings provide new insights for drug development in treating type 2 diabetes.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Food Science & Technology
Hiroyuki Hattori, Takashi Mori, Takahiro Shibata, Masaki Kita, Tohru Mitsunaga
Summary: This study found that 6-paradol, a major vanilloid found in GOP, has anti-obesity effects by decreasing body weight gain and visceral and subcutaneous fats. It also suppresses hepatic cholesterol and triglyceride levels, and decreases gene expression related to fatty acid synthesis, lipid transportation, and adipocyte differentiation in both liver and adipose tissue. Additionally, 6-gingerol promoted phosphorylation of AMP-activated protein kinase (AMPK) while 6-paradol did not, suggesting that 6-paradol regulates obesity-related genes in an AMPK-independent manner.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Moeri Oshimo, Fumie Nakashima, Kenji Kai, Hirofumi Matsui, Takahiro Shibata, Mitsugu Akagawa
Summary: Sulfites, commonly used as preservatives and antioxidants in the food industry, have been shown to induce significant cytotoxicity in rat gastric mucosal cells through oxidative stress, forming protein carbonyls and 8-hydroxy-2'-deoxyguanosine. This indicates potential gastric tissue injury via formation of sulfite-related free radicals.
FREE RADICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Kosuke Yamaguchi, Masanori Itakura, Roma Kitazawa, Sei-Young Lim, Koji Nagata, Takahiro Shibata, Mitsugu Akagawa, Koji Uchida
Summary: The study found that polyphenols, especially catechol-type polyphenols, exhibit lysyl oxidase-like activity and mediate oxidative deamination of lysine residues in proteins. The oxidation products of lysine were identified, and it was observed that the reaction was mediated by some polyphenols, especially o-diphenolic-type polyphenols, in the presence of copper ions. The natural immunoglobulin M monoclonal antibody recognized these oxidation products, indicating a potential role in innate immune response.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Hiromasa Tanaka, Yugo Hosoi, Kenji Ishikawa, Jun Yoshitake, Takahiro Shibata, Koji Uchida, Hiroshi Hashizume, Masaaki Mizuno, Yasumasa Okazaki, Shinya Toyokuni, Kae Nakamura, Hiroaki Kajiyama, Fumitaka Kikkawa, Masaru Hori
Summary: The study identified novel chemical products in plasma-activated L-sodium lactate solution that exhibited selective cytotoxic effects on cancer cells, shedding light on the components responsible for the selective killing of cancer cells. This provides useful data for potential development of cancer treatments using plasma-activated solutions.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Kazuhiro Nishiyama, Akiyuki Nishimura, Kakeru Shimoda, Tomohiro Tanaka, Yuri Kato, Takahiro Shibata, Hiroshi Tanaka, Hitoshi Kurose, Yasu-Taka Azuma, Hideshi Ihara, Yoshito Kumagai, Takaaki Akaike, Philip Eaton, Koji Uchida, Motohiro Nishida
Summary: This study identified a mechanism called redox-dependent alternative internalization (REDAI) that promotes the internalization and degradation of the purinergic P2Y(6) receptor (P2Y(6)R). The researchers found that both synthetic and natural compounds containing electrophilic isothiocyanate groups can modify P2Y(6)R and facilitate its internalization and degradation. This mechanism, mediated by cysteine modification, may also play a role in the internalization of other G protein-coupled receptors (GPCRs) and potentially limit the progression of colitis.
Article
Chemistry, Multidisciplinary
Remi Kinoshita, Ikko Kozaki, Kazunori Shimizu, Takahiro Shibata, Akihito Ochiai, Hiroyuki Honda
Summary: A method for synthesizing a free cyclic peptide library was established via peptide array synthesis, demonstrating sequence activity of cyclic peptides. Variants of oxytocin were systematically evaluated, with some showing high antagonistic activity. Notably, the Y2W variant exhibited the highest inhibitory effect.
Article
Multidisciplinary Sciences
Masanori Itakura, Kosuke Yamaguchi, Roma Kitazawa, Sei-Young Lim, Yusuke Anan, Jun Yoshitake, Takahiro Shibata, Lumi Negishi, Hikari Sugawa, Ryoji Nagai, Koji Uchida
Summary: Reducing sugars can react with proteins to form advanced glycation end products (AGEs), which are pathogenic molecules involved in inflammation and human diseases. This study identifies histone as a cell-surface receptor for AGEs and suggests that AGEs may also play a role in homeostatic response by binding to histone.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Jun Yoshitake, Mayuko Azami, Haruka Sei, Daisuke Onoshima, Kumiko Takahashi, Akiyoshi Hirayama, Koji Uchida, Yoshinobu Baba, Takahiro Shibata
Summary: Extracellular vesicles are nanoscale lipid bilayer vesicles released by almost all cell types. They play an important role in cell-cell communication and have potential applications in disease diagnosis and drug delivery. In this study, a size-exclusion chromatography column device was developed for rapid and effective isolation of extracellular vesicles.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Fumie Nakashima, Juan A. Gimenez-Bastida, Paula B. Luis, Sai H. Presley, Robert E. Boer, Manuel Chiusa, Takahiro Shibata, Gary A. Sulikowski, Ambra Pozzi, Claus Schneider
Summary: Consecutive oxygenation of arachidonic acid by 5-lipoxygenase and cyclooxygenase-2 produces hemiketal ei-cosanoids, HKE2 and HKD2, which promote angiogenesis by inducing endothelial cell tubulogenesis. This study identifies VEGFR2 as a mediator of HKE2-induced angiogenesis in vitro and in vivo. HKE2 treatment increases the phosphorylation of VEGFR2 and downstream kinases ERK and Akt, while HKE2-mediated effects are blocked by the VEGFR2 inhibitor vatalanib. These findings suggest a potential therapeutic strategy utilizing drugs targeting the arachidonic acid pathway for antiangiogenic therapy.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Takeshi Katayoshi, Yuri Kusano, Takahiro Shibata, Koji Uchida, Kentaro Tsuji-Naito
Summary: The study found that low-molecular-weight whey proteins can accelerate dermal collagen production through the TβR/Smad pathway, with components such as beta-lactoglobulin and alpha-lactalbumin playing a role in this process, highlighting their therapeutic potential in wound healing.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Suguru Ariyoshi, Yusuke Imazu, Ryuji Ohguri, Ryo Katsuta, Arata Yajima, Takahiro Shibata, Makoto Ojika
Summary: This study identified two biosynthetic intermediates, 11- and 16-hydroxyphytols, for the mating hormone alpha 2 in the plant pathogen Phytophthora. The results suggest that alpha 2 is possibly synthesized by two cytochrome P450 oxygenases via two hydroxyphytol intermediates in A2 hyphae and secreted outside.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)