Article
Cell Biology
Victor Krajka, Maximilian Naujock, Martje G. Pauly, Felix Stengel, Britta Meier, Nancy Stanslowsky, Christine Klein, Philip Seibler, Florian Wegner, Philipp Capetian
Summary: Differentiating hiPSCs into BFCNs using specific protocols resulted in cells displaying mature immune phenotype and electrophysiological properties, showing potential for modeling neurological diseases such as Alzheimer's and Dystonia.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Reproductive Biology
Yu Jiang, Ning-Ning Cai, Xing-Lan An, Wen-Qian Zhu, Rui Yang, Bo Tang, Zi-Yi Li, Xue-Ming Zhang
Summary: This study compared the effects of mouse embryonic fibroblasts (MEF) and bovine embryonic fibroblasts (BEF) as feeder cells on the culture of bovine induced pluripotent stem cells (iPSCs). The results showed that both MEF and BEF feeder cells were able to successfully convert the bovine iPSCs to a naive-like state. The use of BEF feeder cells in the naive medium (BNM) condition resulted in better maintenance of pluripotency and differentiation potential, and improved conversion efficiency.
Article
Environmental Sciences
M. Chiara Perego, Benjamin D. Mcmichael, Nicholas R. Mcmurry, Scott W. Ventrello, Lisa J. Bain
Summary: Exposure to arsenic during embryogenesis impairs neurodevelopment and locomotor activity. In this study, human-induced pluripotent stem cells were differentiated into motor neurons and exposed to environmentally relevant arsenic concentrations. The results showed that arsenic exposure dysregulated the differentiation of human induced pluripotent stem cells into motor neurons and impaired the cholinergic synapse pathway, suggesting impaired cholinergic function in motor neurons.
Article
Biotechnology & Applied Microbiology
Yunlong Tao, Xueyan Li, Qiping Dong, Linghai Kong, Andrew J. Petersen, Yuanwei Yan, Ke Xu, Seth Zima, Yanru Li, Danielle K. Schmidt, Melvin Ayala, Sakthikumar Mathivanan, Andre M. M. Sousa, Qiang Chang, Su-Chun Zhang
Summary: Researchers have successfully generated central norepinephrine (NE) neurons from stem cells, providing a tool for studying their roles in psychiatric and neurodegenerative diseases.
NATURE BIOTECHNOLOGY
(2023)
Article
Cell Biology
Ying Zhang, Jun Wei, Jiani Cao, Kehua Zhang, Yaojin Peng, Hongkui Deng, Jiuhong Kang, Guangjin Pan, Yong Zhang, Boqiang Fu, Shijun Hu, Jie Na, Yan Liu, Lei Wang, Lingmin Liang, Huanxin Zhu, Yu Zhang, Zi-Bing Jin, Jie Hao, Aijin Ma, Tongbiao Zhao, Junying Yu
Summary: "Requirements for Human-Induced Pluripotent Stem Cells" is the first set of guidelines in China on human-induced pluripotent stem cells. It provides detailed technical requirements and aims to promote international standardization.
CELL PROLIFERATION
(2022)
Article
Biochemistry & Molecular Biology
Xuehui Fan, Lukas Cyganek, Katja Nitschke, Stefanie Uhlig, Philipp Nuhn, Karen Bieback, Daniel Duerschmied, Ibrahim El-Battrawy, Xiaobo Zhou, Ibrahim Akin
Summary: This study compared the electrophysiological and functional properties of endothelial cells derived from human induced pluripotent stem cells with primary human cardiac microvascular endothelial cells. The results demonstrated that the two types of endothelial cells were similar in terms of ion channel function, membrane receptor signaling, and physiological cell functions, although some differences were observed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Pharmacology & Pharmacy
Michelle Vanessa Kapchoup Kamga, Michael Reppel, Jurgen Hescheler, Filomain Nguemo
Summary: Long QT syndrome, Brugada syndrome, and catecholaminergic polymorphic ventricular tachycardia are genetic heart diseases caused by mutations in cardiac ion channels, leading to paroxysmal arrhythmias with different clinical manifestations and functional perturbations. Abnormal sodium and calcium signaling plays a crucial role in these diseases. Patient-specific in vitro models based on induced pluripotent stem cells can help address the underlying mechanisms and develop personalized therapies.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Biochemical Research Methods
Julia Vallverdu, Raquel A. Martinez Garcia de la Torre, Inge Mannaerts, Stefaan Verhulst, Ayla Smout, Mar Coll, Silvia Arino, Teresa Rubio-Tomas, Beatriz Aguilar-Bravo, Celia Martinez-Sanchez, Delia Blaya, Catherine M. Verfaillie, Leo A. van Grunsven, Pau Sancho-Bru
Summary: Human iPSCs are differentiated into HSCs with growth factors for in vitro modeling. The protocol yields iPSC-HSCs with phenotypic and functional characteristics of primary HSCs, suitable for high-throughput in vitro studies. Coculturing iPSC-HSCs with hepatocytes allows for the formation of 3D hepatic spheroids, enabling modeling and drug screening studies.
Article
Biochemistry & Molecular Biology
Gareth Chapman, Mouhamed Alsaqati, Sharna Lunn, Tanya Singh, Stefanie C. Linden, David E. J. Linden, Marianne B. M. van den Bree, Mike Ziller, Michael J. Owen, Jeremy Hall, Adrian J. Harwood, Yasir Ahmed Syed
Summary: Copy Number Variation (CNV) at the 1q21.1 locus is associated with a range of neurodevelopmental and psychiatric disorders in humans, with deletion or duplication of this locus leading to reciprocal phenotypes in neuronal development. These differences are also conserved in a mouse model of 1q21.1 deletion, indicating potential for drug interventions through targeting calcium channels in neurons with 1q21.1 deletion or duplication.
MOLECULAR PSYCHIATRY
(2022)
Review
Neurosciences
Kadla R. Rosholm, Beatrice Badone, Stefania Karatsiompani, David Nagy, Fitzwilliam Seibertz, Niels Voigt, Damian C. Bell
Summary: This article discusses the reality and potential of cellular time travel, as well as the importance of human induced pluripotent stem cells (hiPSCs) in physiology and medicine research. It also explores the application of high throughput automated patch clamp (APC) ion channel recording technology in drug discovery.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Tianyuan Shi, Martin Cheung
Summary: Using rodents for modeling neurological diseases has limitations due to differences in genetics, neural development, and physiology between humans and rodents. In the past decade, induced pluripotent stem cells and induced neural stem cells generated through reprogramming somatic cells have offered a powerful alternative for studying disease pathogenesis and testing regenerative medicines. Urine-derived stem cells are an ideal cell source for reprogramming due to their proliferation, multipotency, epithelial nature, and ease of reprogramming.
CELL AND BIOSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Yue Su, Ling Wang, Zhiqiang Fan, Ying Liu, Jiaqi Zhu, Deborah Kaback, Julia Oudiz, Tayler Patrick, Siu Pok Yee, Xiuchun (Cindy) Tian, Irina Polejaeva, Young Tang
Summary: This study successfully generated biPSCs with long-term self-renewal capacity and pluripotent characteristics from bovine mesenchymal stem cells. The cells exhibited morphological changes under different culture conditions, and transcriptome analysis showed high similarity to bovine ESCs and embryos. The naive-like biPSCs also demonstrated the ability to integrate into extra-embryonic tissues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Hong Cao, Qian Zhou, Chungeng Liu, Yecen Zhang, Minghui Xie, Weihua Qiao, Nianguo Dong
Summary: This study investigates the effect of substrate stiffness on the differentiation of human-induced pluripotent stem cells (h-iPSCs) into valve endothelial-like cells (h-iVECs). The results show that moderate substrate stiffness can enhance the differentiation efficiency of h-iVECs and is regulated by the WNT/Calcineurin signaling pathway. This study emphasizes the importance of using substrate stiffness to achieve a more specific and mature differentiation of h-iVECs.
ACTA BIOMATERIALIA
(2022)
Article
Cell Biology
T. Hartung, M. Rhein, N. Kalmbach, N. Thau-Habermann, M. Naujock, L. Mueschen, H. Frieling, J. Sterneckert, A. Hermann, F. Wegner, S. Petri
Summary: ALS is a rapidly progressive disease affecting motor neurons, and epigenetic modifications of gene expression have been identified as potential mechanisms. This study found significant differences in FUS gene expression and promotor methylation in mutant FUS motor neurons compared to healthy controls, suggesting that targeting disturbed methylation could potentially be a novel therapeutic strategy for ALS.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Neurosciences
Dwaipayan Adhya, Vivek Swarup, Roland Nagy, Lucia Dutan, Carole Shum, Eva P. Valencia-Alarcon, Kamila Maria Jozwik, Maria Andreina Mendez, Jamie Horder, Eva Loth, Paulina Nowosiad, Irene Lee, David Skuse, Frances A. Flinter, Declan Murphy, Grainne McAlonan, Daniel H. Geschwind, Jack Price, Jason Carroll, Deepak P. Srivastava, Simon Baron-Cohen
Summary: The study suggests that autism-iPSCs diverge from control-iPSCs at a cellular level during early neurodevelopment, indicating unique developmental differences associated with autism may be established at early prenatal stages.
BIOLOGICAL PSYCHIATRY
(2021)
Article
Cell Biology
Stephen Gilmore-Hall, Jennifer Kuo, Jacqueline M. Ward, Rabaab Zahra, Richard S. Morrison, Guy Perkins, Albert R. La Spada
JOURNAL OF CELL BIOLOGY
(2019)
Article
Cell Biology
Genea Edwards, Guy A. Perkins, Keun-Young Kim, YeEun Kong, Yonghoon Lee, Soo-Ho Choi, Liu Yujia, Dorota Skowronska-Krawczyk, Robert N. Weinreb, Linda Zangwill, Stefan Strack, Won-Kyu Ju
CELL DEATH & DISEASE
(2020)
Article
Biochemistry & Molecular Biology
Verena Weissert, Bettina Rieger, Silke Morris, Tasnim Arroum, Olympia Ekaterini Psathaki, Thomas Zobel, Guy Perkins, Karin B. Busch
Summary: IF1 modulates both the activity of ATP synthase and the structure and function of mitochondria. Overexpression of IF1 and constitutively active IF1-H49K induced changes in the localization and mobility of ATP synthase, as observed through single molecule tracking and localization microscopy (TALM). Additionally, higher levels of active IF1 resulted in gradual alterations in mitochondrial ultrastructure and function, including significant changes in cristae structures due to the inhibition of the hydrolase activity of the F1FO-ATP synthase by IF1.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2021)
Article
Neurosciences
Guy Perkins, Jeong Han Lee, Seojin Park, Mincheol Kang, Maria C. Perez-Flores, Saeyeon Ju, Grady Phillips, Anna Lysakowski, Michael Anne Gratton, Ebenezer N. Yamoah
JOURNAL OF NEUROSCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Soo-Ho Choi, Keun-Young Kim, Guy A. Perkins, Sebastien Phan, Genea Edwards, Yining Xia, Jungsu Kim, Dorota Skowronska-Krawczyk, Robert N. Weinreb, Mark H. Ellisman, Yury Miller, Won-Kyu Ju
Article
Clinical Neurology
Craig M. McDonald, Israel Ramirez-Sanchez, Bjorn Oskarsson, Nanette Joyce, Candace Aguilar, Alina Nicorici, Jonathan Dayan, Erica Goude, R. Ted Abresch, Francisco Villarreal, Guillermo Ceballos, Guy Perkins, Sundeep Dugar, George Schreiner, Erik K. Henricson
Summary: The study highlights the short-term positive effects of (-)-epicatechin on tissue biomarkers related to mitochondrial biogenesis and muscle regeneration in patients with BMD. Additionally, improvements were observed in graded exercise testing parameters after treatment with (-)-epicatechin.
Article
Multidisciplinary Sciences
Arya Iranmehr, Tsering Stobdan, Dan Zhou, Huiwen Zhao, Sergey Kryazhimskiy, Vineet Bafna, Gabriel G. Haddad
Summary: Using experimental evolution with fruit flies to analyze adaptation to extreme oxygen conditions, researchers found surprising synchronicity in selective sweeps, de novo mutations, and adaptive recombination events.
NATURE COMMUNICATIONS
(2021)
Article
Genetics & Heredity
Dan Zhou, Tsering Stobdan, DeeAnn Visk, Jin Xue, Gabriel G. Haddad
Summary: The study elucidates the cellular and molecular mechanisms underlying tolerance to low oxygen environments, with a focus on the evolutionarily conserved mechanisms of Notch signaling pathway in regulating hypoxia tolerance. Through comparative genomic analysis of Drosophila and high-altitude populations, interactions between Notch signaling and bnl, croc, and Mkk4 were found to be crucial for hypoxia tolerance. These genetic mechanisms are potentially novel therapeutic targets with the ability to be translated to humans for treating/preventing hypoxia-related diseases.
G3-GENES GENOMES GENETICS
(2021)
Article
Physiology
Carsten Mim, Guy Perkins, Gerhard Dahl
Summary: Panx1 plays a crucial role in various physiological and pathological contexts, functioning as a transmembrane pathway for ATP and a highly selective membrane channel for chloride ions.
JOURNAL OF GENERAL PHYSIOLOGY
(2021)
Article
Neurosciences
Rachel Mendelsohn, Guadalupe C. Garcia, Thomas M. Bartol, Christopher T. Lee, Priya Khandelwal, Emily Liu, Donald J. Spencer, Adam Husar, Eric A. Bushong, Sebastien Phan, Guy Perkins, Mark H. Ellisman, Alexander Skupin, Terrence J. Sejnowski, Padmini Rangamani
Summary: This study analyzed mitochondrial morphology in mouse cerebellum neuropil neurons using 3D tracing and differential geometry methods, revealing the impact of mitochondrial structure on cellular metabolic output. These findings contribute to future modeling studies of mitochondrial physiology and metabolism in neurons.
JOURNAL OF COMPARATIVE NEUROLOGY
(2022)
Article
Multidisciplinary Sciences
Lu Ren, Raghavender R. Gopireddy, Guy Perkins, Hao Zhang, Valeriy Timofeyev, Yankun Lyu, Daphne A. Diloretto, Pauline Trinh, Padmini Sirish, James L. Overton, Wilson Xu, Nathan Grainger, Yang K. Xiang, Elena N. Dedkova, Xiao-Dong Zhang, Ebenezer N. Yamoah, Manuel F. Navedo, Phung N. Thai, Nipavan Chiamvimonvat
Summary: SAN dysfunction in heart failure (HF) is well documented, but the underlying mechanisms are not completely understood. This study demonstrates that alterations in the mitochondria-SR microdomains contribute to SAN dysfunction in HF, providing insights into the role of mitochondria-SR microdomains in SAN automaticity and potential therapeutic targets for HF patients.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Shawn Erin McGlynn, Guy Perkins, Min Sub Sim, Mason Mackey, Thomas J. Deerinck, Andrea Thor, Sebastien Phan, Daniel Ballard, Mark H. Ellisman, Victoria J. Orphan
Summary: Working with methane oxidizing ANME archaea and sulfate-reducing bacteria, researchers discovered that their intracytoplasmic membranes (ICMs) resemble the lamellar cristae found in metazoan mitochondria. This suggests a similar functional structure in these microorganisms. Furthermore, similar structures were also found in other bacteria, expanding our knowledge of microbial cell structure in the environment.
Review
Cell Biology
Tonking Bastola, Guy A. Perkins, Keun-Young Kim, Seunghwan Choi, Jin-Woo Kwon, Ziyao Shen, Stefan Strack, Won-Kyu Ju
Summary: AKAP1 is a multifunctional mitochondrial scaffold protein that regulates mitochondrial dynamics, bioenergetics, and calcium homeostasis. Loss of AKAP1 leads to mitochondrial fragmentation and loss of retinal ganglion cells. Modulation of AKAP1 could be a potential therapeutic target for glaucoma and other mitochondria-associated optic neuropathies.
Article
Medicine, Research & Experimental
Priti Azad, Dan Zhou, Hung-Chi Tu, Francisco C. Villafuerte, David Traver, Tariq M. Rana, Gabriel G. Haddad
Summary: This study identified and validated a group of lncRNAs that regulate erythropoiesis in chronic mountain sickness (CMS) through RNA-Seq. HIKER/LINC02228 was found to play a critical role in erythropoiesis in CMS cells by modulating CSNK2B under hypoxia. Pharmacologic inhibition of CSNK2B reduced erythroid colonies, highlighting the importance of HIKER in regulating erythropoiesis.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Multidisciplinary Sciences
Mita Varghese, Victoria A. Kimler, Fariha R. Ghazi, Gurnoor K. Rathore, Guy A. Perkins, Mark H. Ellisman, James G. Granneman
SCIENTIFIC REPORTS
(2019)
Article
Neurosciences
Yang He, Jun Tang, Meng Zhang, Junjie Ying, Dezhi Mu
Summary: This study investigated the protective effects and mechanisms of human placenta derived mesenchymal stem cells (hPMSCs) transplantation in a rat model of hypoxic-ischemic encephalopathy (HIE). The results showed that hPMSCs transplantation reduced apoptosis and improved long-term neurological prognosis. Furthermore, the downregulation of Sema 3A/NRP-1 expression and activation of the PI3K/Akt/mTOR signaling pathway played a key role in the protective effects of hPMSCs.
Article
Neurosciences
Emily L. Isenstein, Edward G. Freedman, Jiayi Xu, Ian A. DeAndrea-Lazarus, John J. Foxe
Summary: This study evaluated electrophysiological discrimination of parametric somatosensory stimuli in healthy young adults to understand how the brain processes the duration of tactile information. The results showed that participants did not electrophysiologically discriminate between 100 and 115 ms, but they exhibited distinct electrophysiological responses when the deviant stimuli were 130, 145, and 160 ms. These findings contribute to a better understanding of tactile sensitivity in different clinical conditions.
Article
Neurosciences
Juliana R. Souza, Ludmila Lima-Silveira, Daniela Accorsi-Mendonca, Benedito H. Machado
Summary: This study demonstrates that A2A receptors play a crucial role in modulating synaptic transmission in the NTS neurons and are required for the enhancement of glutamatergic transmission observed under short-term sustained hypoxia conditions.
Article
Neurosciences
Miki Hashizume, Rina Ito, Rie Suge, Yasushi Hojo, Gen Murakami, Takayuki Murakoshi
Summary: The basolateral amygdaloid complex (BLA) is closely involved in the formation of emotional memories, including both aversive memory and contextual fear memory. Acute sleep deprivation (SD) disrupts the acquisition of tone-associated fear memory in juvenile rats, but has no significant effect on contextual fear memory. Slow network oscillation in the amygdala contributes to the formation of amygdala-dependent fear memory in relation to sleep.
Article
Neurosciences
Qunxian Wang, Shipeng Guo, Dongjie Hu, Xiangjun Dong, Zijun Meng, Yanshuang Jiang, Zijuan Feng, Weihui Zhou, Weihong Song
Summary: GSDME plays a crucial role in the pathogenesis of Alzheimer's disease by regulating the switch from apoptosis to pyroptosis and participating in neuroinflammatory response. Knockdown of GSDME has been shown to improve cognitive impairments, indicating that GSDME could be a therapeutic target for Alzheimer's disease.