Article
Biochemistry & Molecular Biology
Vancha Harish, Waleed Hassan Almalki, Ahmed Alshehri, Abdulaziz Alzahrani, Sami Alzarea, Imran Kazmi, Monica Gulati, Devesh Tewari, Dinesh Kumar Chellappan, Gaurav Gupta, Kamal Dua, Sachin Kumar Singh
Summary: The present study developed a bioanalytical method for the estimation of Xanthohumol (XH) in rat plasma using high performance liquid chromatography. The method validation was performed according to ICH M10 guidelines, and the results showed that the method was precise and accurate. The developed method can be effectively used for the estimation of XH in plasma samples.
Article
Chemistry, Analytical
Said A. H. Hassan, Aya A. Helmy, Soheir F. Weshahy, Nadia A. Youssef, Badr El-Zeany
Summary: Common antiepileptic drugs have complex pharmacokinetic characteristics that require therapeutic drug monitoring (TDM). A bioanalytical method was developed to measure the plasma concentrations of PER and CAR in combination for TDM. The method successfully determined PER and CAR within their therapeutic levels, and the results met the validation criteria.
JOURNAL OF ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Wenbo Ren, Lei Yin, Gaixia Zhang, Taiyu Zhai, Jing Huang
Summary: This study developed and validated a high-performance liquid chromatography tandem mass spectrometry cubed (HPLC-MS3) method for quantifying voriconazole in human plasma. The method showed high accuracy and stability, and compared to HPLC-MRM, HPLC-MS3 had a higher signal-to-noise ratio and response without significant differences in results. The HPLC-MS3 method was successfully applied to monitor voriconazole therapeutic drug monitoring (TDM) in patients, confirming the implementation of dosing guidelines and the effectiveness of treatment.
Article
Infectious Diseases
Ute Chiriac, Heike Rau, Otto R. Frey, Anka C. Roehr, Sabrina Klein, Anna L. Meyer, Benedict Morath
Summary: Dalbavancin is a promising alternative for the treatment of gram-positive infections that require long-term antibiotic treatment. A simple, rapid, and cost-effective HPLC-UV method for quantification of dalbavancin has been developed and validated. This method allows for accurate and selective monitoring of dalbavancin levels in human serum.
Article
Biochemistry & Molecular Biology
Vanesa Escudero-Ortiz, Francisco Jose Rodriguez-Lucena, Gabriel Estan-Cerezo, Esther Mancheno-Macia, Venancio Conesa-Garcia, Ana Garcia-Monsalve, Leticia Soriano-Irigaray, Andres Navarro-Ruiz
Summary: This study aimed to develop and validate a selective and precise high performance liquid chromatography-ultraviolet method for the measurement of nilotinib in plasma from patients with chronic myeloid leukemia. The method demonstrated sensitivity, selectivity, reproducibility, and rapidity, making it suitable for pharmacokinetic studies and therapeutic drug monitoring in routine clinical practice.
Article
Plant Sciences
Rubiya Khursheed, Sheetu Wadhwa, Bimlesh Kumar, Monica Gulati, Saurabh Gupta, M. V. N. L. Chaitanya, Deepak Kumar, Niraj Kumar Jha, Gaurav Gupta, Parteek Prasher, Dinesh Kumar Chellappan, Kamal Dua, Sachin Kumar Singh
Summary: Curcumin and quercetin are important flavonoids with various pharmacological activities. A bioanalytical method for simultaneous estimation of both drugs in rat's plasma was developed and validated. The method showed accuracy, precision, and stability, indicating its effectiveness for estimating curcumin and quercetin in rat's plasma.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Infectious Diseases
Julia Zimmer, Anka C. Roehr, Stefan Kluge, Jonas Faller, Otto R. Frey, Dominic Wichmann, Christina Koenig
Summary: Cefiderocol is a new siderophore cephalosporin approved for treating multidrug resistant bacteria, with activity against carbapenem-resistant Enterobacterales and Pseudomonas aeruginosa. A simple, rapid, cost-effective HPLC method was developed and validated for quantifying Cefiderocol in serum, showing good performance in monitoring serum levels and optimizing antibiotic therapy for critically ill patients.
Article
Biochemistry & Molecular Biology
Federica Pigliasco, Alessia Cafaro, Raffaele Simeoli, Sebastiano Barco, Alberto Magnasco, Maura Faraci, Gino Tripodi, Bianca Maria Goffredo, Giuliana Cangemi
Summary: The study developed a new LC-MS/MS micro-method for simultaneous quantification of aciclovir and ganciclovir, with potential applications in pediatric medicine. The assay was accurate and reproducible in both plasma and dried plasma spots, showing comparable results between the two sample types.
Article
Biochemical Research Methods
Shaik Khaja Moinuddin, Pirangi Srikanth, Parul Sharma, Sukhendu Nandi
Summary: Cancer is a complex disease and chemotherapy is considered the best approach for treatment. However, the chemotherapeutic compound doxorubicin (DOX) has poor bioavailability and exhibits cardiotoxicity. The combination of DOX and lapatinib (LAP) has shown to decrease the cardiotoxic effect of DOX. To address the issues of DOX's bioavailability and synergistic effect with LAP, a simple and rapid analytical method was developed.
JOURNAL OF CHROMATOGRAPHIC SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Bartlomiej Sankowski, Sylwia Michorowska, Emilia Rackowska, Mariusz Sikora, Joanna Giebultowicz
Summary: This study aimed to verify the use of saliva in therapeutic drug monitoring (TDM) of teriflunomide, and developed a reliable and robust LC-MS/MS method for quantification of teriflunomide in saliva. The results showed a good linear correlation between the concentration of teriflunomide in plasma and both resting and stimulated saliva, confirming the effectiveness of this non-invasive method of teriflunomide's TDM. Salivary flow and the type of swab in the collector device did not significantly influence teriflunomide concentration in saliva, but the use of stimulated saliva and synthetic swabs is recommended to reduce variability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Pawel K. Kunicki, Aleksandra Wrobel
Summary: The aim of the work was to develop a simple and reliable HPLC-UV method for monitoring mycophenolic acid (MPA) levels. Sample preparation involved plasma protein precipitation with acetonitrile and isocratic separation on a Supelcosil LC-CN column. The method showed satisfactory precision with a calibration range of 0.1-40 μg/mL.
Article
Pharmacology & Pharmacy
Giovanni Canil, Marco Orleni, Bianca Posocco, Sara Gagno, Alessia Bignucolo, Marcella Montico, Rossana Roncato, Serena Corsetti, Michele Bartoletti, Giuseppe Toffoli
Summary: Poly (ADP-ribose) polymerase inhibitors (PARPis) are increasingly important in oncology, and their therapeutic drug monitoring (TDM) could benefit patients. This study developed and validated a liquid chromatography-tandem mass spectrometry method for quantifying olaparib, rucaparib, and niraparib in both human plasma and dried blood spot (DBS). The correlation between drug concentrations measured in these two matrices was assessed. Results showed a strong correlation between plasma and DBS for olaparib and niraparib, though further data is needed to establish a robust regression analysis for rucaparib. The study provides a solid foundation for the feasibility of using both plasma and DBS matrices for PARPis TDM.
Article
Biochemical Research Methods
Raymond E. West III, Patrick J. Oberly, Sharon A. Riddler, Thomas D. Nolin, Aaron S. Devanathan
Summary: A highly selective UHPLC-MS/MS method was developed and validated for quantification of Lenacapavir concentrations in human plasma. This method can be used for monitoring the therapeutic efficacy of HIV treatment.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Biochemical Research Methods
Zhi-mei Yang, Ya-bin Qin, De-yun Zhao, Lan-tao Wang, Ying-ping Tian, Jian-fang Liu
Summary: This study developed a simple and sensitive LC-MS/MS method for therapeutic drug monitoring of digoxin in children with a lower limit of quantitation of 0.2 ng/mL based on 30 μL of plasma. The plasma sample was pretreated using one-step protein precipitation, followed by chromatographic separation on a short C-18 column with a total run time of 2.4 min. Detection was achieved through multiple reaction monitoring using positive ionization mode on a triple quadrupole mass spectrometer. The method was fully validated and applied to determine plasma digoxin concentrations in 43 pediatric patients, proving its appropriateness for routine clinical laboratory practice.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Chemistry, Analytical
Bruno Charlier, Albino Coglianese, Francesca Felicia Operto, Federica De Rosa, Francesca Mensitieri, Giangennaro Coppola, Amelia Filippelli, Fabrizio Dal Piaz, Viviana Izzo
Summary: Therapeutic drug monitoring is crucial for improving the quality of antiepileptic drug use, especially in specific clinical situations. A novel method for measuring perampanel concentration in plasma samples was developed and compared with a commercially available method, showing the potential for clinical routine use as an alternative to LC-MS/MS.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Review
Pharmacology & Pharmacy
Mariana Matias, Adriana O. O. Santos, Samuel Silvestre, Gilberto Alves
Summary: Epilepsy is a chronic and complex neurological disease affecting approximately 50 million people worldwide. Despite the introduction of numerous new antiseizure drugs, many patients still suffer from uncontrolled seizures, necessitating the development of more effective therapies. Nanomedicine offers a promising approach to deliver drugs to the brain, enhancing their therapeutic potential. This review addresses the general features and management of epilepsy, as well as the challenges in developing better antiseizure therapies. It also discusses the role of nanomedicines in selectively delivering drugs, overcoming physical-chemical limitations, improving brain penetration, reducing adverse effects, and circumventing drug resistance.
Article
Pharmacology & Pharmacy
Soraia Silva, Joana Bicker, Amilca Falcao, Ana Fortuna
Summary: The intranasal route is being explored for systemic drug delivery and treatment of pulmonary and neurological diseases. The air-liquid interface (ALI) method promotes cell differentiation and optimization of airway epithelium, providing relevant input for inhalation and pulmonary toxicology. However, there is currently no consensus on the validation of the ALI method, hampering data comparison.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Ivanna Hrynchak, Diana Cocioaba, Alexandra I. Fonseca, Radu Leonte, Sergio J. C. do Carmo, Roxana Cornoiu, Amilcar Falcao, Dana Niculae, Antero J. Abrunhosa
Summary: Antibody and nanobody-based copper-64 radiopharmaceuticals are proposed as theranostic tools in human diseases. This study discusses the production, purification, and radiolabeling of antibodies and nanobodies using copper-64 obtained from both solid and liquid targets. The liquid target method is practical and reliable, offering advantages such as speed, ease of automation, and continuous production.
Article
Chemistry, Multidisciplinary
Ana C. Goncalves, Ana R. Nunes, Sara Meirinho, Miguel Ayuso-Calles, Rocio Roca-Couso, Raul Rivas, Amilcar Falcao, Gilberto Alves, Luis R. Silva, Jose David Flores-Felix
Summary: This study analyzes the biological potential of blueberries and sweet cherries in several dimensions of human health, emphasizing the research on the benefits of consuming these fruits. Through the phenolic profile analysis of phenolic-enriched extracts from blueberries and sweet cherries, their antioxidant potential and ability to inhibit α-glucosidase enzyme activity were evaluated, along with their antimicrobial activity. The results demonstrate the significant antioxidant and antimicrobial properties of blueberries and sweet cherries, which are correlated with the presence of phenolic compounds.
APPLIED SCIENCES-BASEL
(2023)
Article
Pharmacology & Pharmacy
Sara Meirinho, Marcio Rodrigues, Adriana O. Santos, Amilcar Falcao, Gilberto Alves
Summary: Stiripentol is a new-generation antiepileptic drug that can only be administered orally. However, it is unstable in acidic environments and dissolves slowly and incompletely in the gastrointestinal tract. This study developed intranasal microemulsions to improve the delivery of Stiripentol. Compared to oral administration, intranasal administration significantly increased the plasma and brain concentrations of Stiripentol, suggesting that it could be a promising alternative for clinical testing.
Article
Pharmacology & Pharmacy
Rui Silva, Helena Colom, Joana Bicker, Anabela Almeida, Ana Silva, Francisco Sales, Isabel Santana, Amilcar Falcao, Ana Fortuna
Summary: This study aimed to develop a population pharmacokinetic (PopPK) model for perampanel in patients with refractory epilepsy. The results showed that a one-compartment model with first-order elimination best described the pharmacokinetic profiles of perampanel. Covariates such as enzyme-inducing antiepileptic drugs (EIAEDs) and body mass index (BMI) were found to significantly affect the clearance and volume of distribution of perampanel.
Article
Infectious Diseases
Maria Aquino, Maria Tinoco, Joana Bicker, Amilcar Falcao, Marilia Rocha, Ana Fortuna
Summary: Amikacin is the preferred antibiotic for treating Gram-negative infections in neutropenic oncology patients. However, there is a lack of population pharmacokinetic studies on amikacin in this patient population. This large-scale retrospective study investigated the effects of tumor diseases, chemotherapy, lag time, age, and renal function on amikacin pharmacokinetics in neutropenic cancer patients. The results showed that oncology patients received higher daily doses of amikacin and had increased drug clearance. Chemotherapy influenced amikacin pharmacokinetics and drug clearance decreased with longer lag time. The impact of aging on amikacin doses was stronger than chemotherapy. This research suggests that cancer patients may require higher initial doses of amikacin, especially within 30 days of starting chemotherapy.
Review
Chemistry, Medicinal
Ana R. R. Nunes, Elisabete C. C. Costa, Gilberto Alves, Luis R. Silva
Summary: Diabetes mellitus is a metabolic disease characterized by abnormal blood glucose levels caused by a lack of insulin secretion or impaired insulin action. The incidence of diabetes is increasing, resulting in high healthcare costs. Current drugs have side effects, leading to kidney and liver problems. Natural compounds rich in anthocyanidins have potential for the prevention and treatment of diabetes, but their application is hindered by issues such as lack of standardization and poor stability. Nanotechnology can be used for effective delivery of these compounds.
Review
Biochemistry & Molecular Biology
Jordi Olloquequi, Miren Ettcheto, Amanda Cano, Ana Fortuna, Joana Bicker, Elena Sanchez-Lopez, Cristian Paz, Jesus Urena, Ester Verdaguer, Carme Auladell, Antoni Camins
Summary: Licochalcone A (Lico-A) is a potential neuroprotective agent with various mechanisms of action, including enhancing cognitive activity, inhibiting neuroinflammation, reducing tau phosphorylation, increasing acetylcholine levels, decreasing amyloid plaques, and exerting antioxidant effects. Further research on the underlying mechanisms and targets of Lico-A may provide new insights for the treatment of cognitive disorders and neurodegenerative diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Sara Meirinho, Marcio Rodrigues, Adriana O. Santos, Amilcar Falcao, Gilberto Alves
Summary: The intranasal administration of Perampanel (PER) through the olfactory pathway showed improved brain distribution and demonstrated anticonvulsant and anxiolytic effects in mice, indicating a potential alternative to oral treatment for epilepsy and anxiety. However, it also caused some neuromotor impairment, which was improved with repeated administrations. The study supports the design of clinical trials to evaluate the efficacy of intranasal PER delivery in treating neurological-related conditions.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Patricia Henriques, Joana Bicker, Soraia Silva, Slavomira Doktorovov, Ana Fortuna
Summary: In nasal drug product development, it is important to conduct screening studies to select promising compounds or formulations. The Parallel Artificial Membrane Permeability Assay (PAMPA) has been used to evaluate drug permeability across various barriers. This study optimized a new nasal-PAMPA model using a biorelevant medium with mucin, which accurately predicted nasal permeability and showed potential for drug and formulation screening.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Patricia C. Pires, Mariana Fernandes, Francisca Nina, Francisco Gama, Maria F. Gomes, Lina E. Rodrigues, Sara Meirinho, Samuel Silvestre, Gilberto Alves, Adriana O. Santos
Summary: The aim of this study was to develop a nanoemulsion (NE) with good solubilization capacity for lipophilic drugs and the ability to promote the absorption of drugs with low permeability. Compositions containing two lipids were screened and characterized, and one composition showed high droplet size homogeneity without the need for high energy methods. Optimized compositions containing a high proportion of propylene glycol monocaprylate NF were able to solubilize simvastatin at high drug strength and promote faster intranasal absorption of the hydrophilic prodrug fosphenytoin.
Article
Pharmacology & Pharmacy
Patricia Henriques, Joana Bicker, Andreia Carona, Margarida Miranda, Carla Vitorino, Slavomira Doktorovova, Ana Fortuna
Summary: This study investigated the application of amorphous solid dispersions (ASD) for nasal delivery, and compared two particle engineering strategies. The results showed that ASD with hydroxypropyl methylcellulose (HPMC) enhanced drug release and permeation, leading to fast drug absorption. In vitro release and ex vivo permeation studies were found to be predictive tools for nasal absorption.
JOURNAL OF PHARMACEUTICAL INVESTIGATION
(2023)