Article
Engineering, Environmental
Sangeet Adhikari, Rahul Kumar, Erin M. Driver, Tyler D. Perleberg, Allan Yanez, Bridger Johnston, Rolf U. Halden
Summary: This study utilized wastewater-based epidemiology to investigate the use of antimicrobials during the COVID-19 pandemic. The findings indicate an overall increase in antimicrobial use, while the use of TCS and TCC decreased during the pandemic, possibly due to the FDA ban.
Article
Chemistry, Multidisciplinary
Chang Shu, Teng-Fei Li, Duo Li, Zhong-Qiu Li, Xing-Hua Xia
Summary: Pomalidomide, an IMiD approved by the FDA for multiple myeloma treatment, was quantified in human plasma using a validated LC-MS/MS method. The method was successfully applied to clinical pharmacokinetic studies with positive results.
CHINESE CHEMICAL LETTERS
(2022)
Article
Environmental Sciences
Xiaoyong Tian, Kaiqin Huang, Yangyang Liu, Kaixin Jiang, Ranran Liu, Juntao Cui, Fei Wang, Yingxin Yu, Huanhuan Zhang, Meiqing Lin, Shengtao Ma
Summary: This study investigated the distribution of nineteen ingredients of personal care products in paired human hair, nail, and urine samples. The concentrations of mPAEs, BPs, and AAs varied across different matrices and showed significant differences between male and female. These results suggest the need to use multiple matrices to comprehensively assess the risk of ingredients of personal care products to human health.
ENVIRONMENTAL POLLUTION
(2023)
Article
Chemistry, Analytical
Andisiwe Malo, Tracy Kellermann, Elisa H. Ignatius, Kelly E. Dooley, Rodney Dawson, Anton Joubert, Jennifer Norman, Sandra Castel, Lubbe Wiesner
Summary: A method for the extraction and quantification of pretomanid in human plasma using LC-MS/MS was developed and validated. The method showed good performance in accuracy, precision, selectivity, sensitivity, recovery and stability, and was specific for pretomanid.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Microbiology
Xiaofei Wang, Xiaojuan Wang, Tongkai Cai, Yulin Qin, Ling Li, Yuanying Jiang, Bing Han, Yongbing Cao
Summary: In this study, a simple and sensitive liquid chromatography with tandem mass spectrometry (LC-MS/MS) method was developed to quantify the intracellular fluconazole (FLC) content in the opportunistic human fungal pathogen Candida albicans. The method was found to be accurate, efficient, and reproducible, and was successfully validated in 36 clinical isolated strains.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Marjeta Mencin, Maja Mikulic-Petkovsek, Robert Veberic, Petra Terpinc
Summary: A solid-phase extraction (SPE) technique was developed and optimised for isolation and concentration of extractable and bound phenolic acids from germinated spelt seeds, for analysis by liquid chromatography-mass spectrometry. The study found that this method has significant advantages over traditional liquid-liquid extraction in terms of higher extraction efficiencies and can be used for quantitative analysis of extractable and bound phenolic acids.
Article
Biochemical Research Methods
Mihai Victor Curtasu, Natalja P. Norskov
Summary: The present work aims to develop a high-yield extraction procedure combined with a robust, sensitive, and fast LC-MS/MS method for quantifying flavonoids and salicylic acid in Willow bark. The research investigates the effect of freeze and oven-drying procedures and five extraction solvents on the yield of individual flavonoids and salicylic acid. The freeze-drying procedure is the best for preserving monomeric and polymeric flavan-3-ols, while the best extraction solvent is methanol acidified with 1% hydrochloric acid.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemistry & Molecular Biology
Rico Schwarz, Anna Richter, Elisabeth R. D. Ito, Hugo Murua Escobar, Christian Junghanss, Burkhard Hinz
Summary: Silmitasertib is a drug currently being investigated in clinical trials for the treatment of various cancers. It inhibits CK2 protein kinase, which has proliferation-promoting and anti-apoptotic effects on cancer cells. Researchers have developed an LC-MS/MS method for quantifying silmitasertib in human plasma.
Article
Biochemistry & Molecular Biology
Rico Schwarz, Elisabeth R. D. Seiler, Sina Sender, Anahit Pews-Davtyan, Hugo Murua Escobar, Dietmar Zechner, Matthias Beller, Christian Junghanss, Burkhard Hinz
Summary: A sensitive LC-MS/MS method for quantification of the potential anticancer drug PDA-66 in human plasma is presented. This method can be used for monitoring anticancer drug treatment and has been tested in mouse plasma and sera.
Article
Biochemistry & Molecular Biology
Sarah Gregory, Scott G. G. Denham, Patricia Lee, Joanna P. P. Simpson, Natalie Z. M. Homer
Summary: This paper describes the application of an LC-MS/MS steroid analysis method to evaluate the reference ranges of steroids in saliva samples from older adults, and explores the correlation and sex differences of steroids in saliva.
Article
Chemistry, Applied
Bimal Chitrakar, Yakun Hou, Sakamon Devahastin, Min Zhang, Yaxin Sang
Summary: This study investigated the valorization of asparagus leafy by-products as a potential source of rutin through selected extraction and purification protocols. The protocol resulting in the highest extraction yield was chosen, followed by purification via multiple liquid-liquid back extraction using ethanol, methanol, or water. The proposed purification protocol yielded yellow-color crystals, confirmed as rutin through characterization techniques. The purity of the rutin was determined to be 97.6% by ultra-performance liquid chromatography, with a yield of 78.2%. The remaining rutin (21.8%) was found in the collected liquids, which can be recycled using the same purification process. These protocols offer a simple and effective method for rutin extraction and purification from asparagus leafy by-products.
Article
Medicine, Legal
Maria Cobo-Golpe, Ana De-Castro-Rios, Angelines Cruz, Mario Paramo, Manuel Lopez-Rivadulla, Elena Lendoiro
Summary: A method was developed to detect 12 antidepressant and benzodiazepine drugs in hair and nail samples. Results showed that concentrations of most drugs in hair were higher than in nails for patients under treatment, with some exceptions where nail concentrations were lower than toenail concentrations.
FORENSIC SCIENCE INTERNATIONAL
(2021)
Article
Chemistry, Analytical
Jakub Czarny, Jadwiga Musial, Jolanta Powierska-Czarny, Natalia Galant, Michal Raczkowski, Boguslaw Buszewski, Renata Gadzala-Kopciuch
Summary: The development of the black market and the increase in psychoactive substances require the development of effective analytical methods to prevent poisoning and deaths. The study aimed to develop a method for the identification of over 500 psychoactive substances in blood samples.
MICROCHEMICAL JOURNAL
(2022)
Article
Biochemical Research Methods
Samuel Bernardo-Bermejo, Jingchuan Xue, Linh Hoang, Elizabeth Billings, Bill Webb, M. Willy Honders, Sanne Venneker, Bram Heijs, Maria Castro-Puyana, Maria Luisa Marina, Erik B. van den Akker, Marieke Griffioen, Gary Siuzdak, Martin Giera, Elena Sanchez-Lopez
Summary: Analytical techniques with high sensitivity and selectivity are crucial in clinical sample analysis. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is the standard method, but it is expensive. This study demonstrates that similar results can be achieved using a simpler single mass spectrometry detector combined with enhanced in-source fragmentation/annotation and correlated ion monitoring. The protocol provides step-by-step instructions for optimizing the conditions for this technique, making it broadly applicable and cost-effective for quantifying small molecules.
Article
Biochemical Research Methods
Viritha Bezawada, Padma Mogili, Sireesha Dodda, Ramakrishna Gajula, Suhasini Ponnada
Summary: A sensitive and selective liquid chromatography-tandem mass spectrometry method has been developed for the quantification of trifluoperazine in human plasma. The method showed good linearity, accuracy, and precision, and met the validation criteria set by the US Food and Drug Administration.
BIOMEDICAL CHROMATOGRAPHY
(2022)
