Review
Chemistry, Analytical
Tengfei Yuan
Summary: Analyzing steroids in different biological matrices helps gain insight into the status of steroids.
MICROCHEMICAL JOURNAL
(2022)
Article
Environmental Sciences
Michael S. Gross, Emily E. Woodward, Michelle L. Hladik
Summary: The study evaluated the use of an ELISA kit for analyzing imidacloprid in biological samples, showing that ELISA can be used as an effective screening tool but confirmation with more selective techniques is recommended for complex samples.
Article
Biochemical Research Methods
Mathias Langner, Ivona Mateska, Nicole Bechmann, Ben Wielockx, Triantafyllos Chavakis, Vasileia Ismini Alexaki, Mirko Peitzsch
Summary: In this study, a simple LC-MS/MS method is described for accurately quantifying arginine and its related metabolites in different experimental samples. The method is suitable for various applications and shows good performance in quantifying arginine metabolites in cell lysates, cell culture supernatants, and tissue extracts.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Review
Biochemical Research Methods
Anastasia Alexandridou, Dietrich A. Volmer
Summary: This review summarizes sample preparation methods for measurement of vitamin D metabolites using liquid chromatography-(tandem)mass spectrometry in more than ten different human tissues, including hair, saliva, adipose tissue, brain, and others. Researchers have been interested in using alternative sample matrices for the determination of vitamin D metabolites as it allows non-invasive sampling and contributes to a better understanding of vitamin D metabolism and function.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Soil Science
Arindam Ghatak, Florian Schindler, Gert Bachmann, Doris Engelmeier, Prasad Bajaj, Martin Brenner, Lena Fragner, Rajeev K. Varshney, Guntur Venkata Subbarao, Palak Chaturvedi, Wolfram Weckwerth
Summary: Root exudates play a crucial role in shaping soil microbial communities, with genotype-specific responses observed under drought stress. Changes in root exudation under drought stress can influence soil microbial adaptation and survival mechanisms, highlighting the importance of understanding these interactions for plant resilience.
BIOLOGY AND FERTILITY OF SOILS
(2022)
Article
Chemistry, Analytical
Jakub Maculewicz, Piotr Stepnowski, Joanna Dolzonek, Anna Bialk-Bielinska
Summary: Imidazolium ionic liquids (ILs) are increasingly being used, and there is evidence of their presence in the environment. Due to their potential for bio-concentration, it is necessary to monitor the presence of these compounds in organisms from potentially contaminated waters. This study developed a novel analytical procedure for the determination of imidazolium ILs in mussel tissues using a combination of extraction techniques and LC-MS/MS analysis.
Article
Chemistry, Analytical
Devendra Kumar, Nagsen Gautam, Yazen Alnouti
Summary: There are challenges in the development and validation of LC-MS/MS bioanalytical methods, particularly with low and variable recovery of analytes. This article proposes a practical protocol to systematically identify and quantify the sources of low analyte recovery and optimize bioanalytical conditions for improved recovery.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Athina G. Nicolaou, Marios C. Christodoulou, Ioannis J. Stavrou, Constantina P. Kapnissi-Christodoulou
Summary: This review discusses the impact of cannabis abuse on neurocognitive functions and emphasizes the importance of developing analytical methods. The focus is on liquid chromatography mass spectrometry or tandem mass spectrometry as the new golden standard for forensic analysis.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Food Science & Technology
Xuan Zeng, Yuying Zheng, Yan He, Wei Peng, Weiwei Su
Summary: Naringin is a dietary flavonoid glycoside with multiple bioactivities. This study developed a rapid and sensitive method for simultaneous determination of ten flavonoid metabolites of naringin in rat urine. The results revealed the visible individual difference and low urinary recovery of flavonoid metabolites in the excretion of naringin, which may be helpful for further study to understand the in vivo behavior and action mechanism of naringin.
Article
Food Science & Technology
Fulya Tasci, Hale Secilmis Canbay, Mahmut Doganturk
Summary: The study found antibiotic residues in non-compliant milk samples, suggesting that not all milk producers adhere to good veterinary practices.
Article
Chemistry, Analytical
Zhendong Chen, Liang Li, Yan Zhan, Yifan Zhang, Haiyan Liu, Jianjun Zou, Dafang Zhong
Summary: Henagliflozin is a specific inhibitor of SGLT2 proposed for T2DM treatment. This study identified 8 metabolites of henagliflozin in human plasma and urine, focusing on the glucuronidation and O-deethylation pathways. A LC-MS/MS method was developed for quantification of M5-1, M5-2, and M5-3 in human plasma.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Biochemistry & Molecular Biology
Seong Jun Jo, Zhouchi Huang, Chae Bin Lee, Soon Uk Chae, Soo Hyeon Bae, Soo Kyung Bae
Summary: A simple and sensitive LC-MS/MS method was developed and validated for the quantification of Benzisothiazolinone (BIT) in rat plasma, urine, and tissue homogenates. The pharmacokinetics of BIT were evaluated in rats, providing insights into the relationship between BIT exposure and toxicity and a basis for future risk assessment studies in humans.
Article
Chemistry, Multidisciplinary
Tengteng Ping, Min Zheng, Pingping Zhang, Tianhao Yan, Xufeng Miao, Ke Wang, Kaoqi Lian
Summary: An analytical method using a 96-well protein precipitation plate combined with HPLC-MS/MS was developed and validated for the simultaneous determination of 12 anti-obesity drugs in human plasma. This method offers advantages of efficient and rapid analysis, simple operation, high accuracy and sensitivity. It can be used for clinically monitoring adverse reactions and guiding the appropriate use of weight-loss drugs for obese individuals.
Article
Engineering, Environmental
Bibai Du, Quan Li, Zibin Pan, Yun Zhang, Ruiman Xie, Dan Luo, Lixi Zeng
Summary: An improved LC-MS/MS method was developed for simultaneous determination of synthetic phenol antioxidants and relevant metabolites, enhancing sensitivity with APCI and eliminating background contamination by installing a trap column. The method allows for accurate detection of target substances in environmental samples.
ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS
(2021)
Article
Pharmacology & Pharmacy
Shasha Jin, Zhihong Li, Qing Yang, Boyu Fang, Xiaoqiang Xiang, Chao Peng, Weimin Cai
Summary: A sensitive and specific chiral liquid chromatography-tandem mass spectrometry method was developed to simultaneously identify the enantiomers of warfarin and hydroxywarfarins. This method has advantages in baseline resolution and analysis processes.
Article
Oncology
Anne-Charlotte Dubbelman, Cynthia M. Nijenhuis, Robert S. Jansen, Hilde Rosing, Hitoshi Mizuo, Shinki Kawaguchi, David Critchley, Robert Shumaker, Jan H. M. Schellens, Jos H. Beijnen
INVESTIGATIONAL NEW DRUGS
(2016)
Correction
Biochemical Research Methods
A. C. Dubbelman, M. Tibben, H. Rosing, A. Gebretensae, L. Nan, S. H. Gorman, P. Robertson, J. H. M. Schellens, J. H. Beijnen
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2016)
Article
Chemistry, Analytical
Anne-Charlotte Dubbelman, Filip Cuyckens, Lieve Dillen, Gerhard Gross, Rob J. Vreeken, Thomas Hankemeier
ANALYTICA CHIMICA ACTA
(2018)
Article
Oncology
Anne-Charlotte Dubbelman, Hilde Rosing, Cynthia Nijenhuis, Alwin D. R. Huitema, Marja Mergui-Roelvink, Anubha Gupta, David Verbel, Gary Thompson, Robert Shumaker, Jan H. M. Schellens, Jos H. Beijnen
INVESTIGATIONAL NEW DRUGS
(2015)
Article
Biochemical Research Methods
Anne-Charlotte Dubbelman, Filip Cuyckens, Lieve Dillen, Gerhard Gross, Thomas Hankemeier, Rob J. Vreeken
JOURNAL OF CHROMATOGRAPHY A
(2014)
Article
Biochemical Research Methods
J. J. M. A. Hendrikx, A. C. Dubbelman, H. Rosing, A. H. Schinkel, J. H. M. Schellens, J. H. Beijnen
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2013)
Article
Pharmacology & Pharmacy
Anne-Charlotte Dubbelman, Hilde Rosing, Mona Darwish, Denise D'Andrea, Mary Bond, Edward Hellriegel, Philmore Robertson, Jos H. Beijnen, Jan H. M. Schellens
Article
Multidisciplinary Sciences
Tom van der Laan, Tim Kloots, Marian Beekman, Alida Kindt, Anne-Charlotte Dubbelman, Amy Harms, Cornelia M. van Duijn, P. Eline Slagboom, Thomas Hankemeier
SCIENTIFIC REPORTS
(2019)
Article
Chemistry, Analytical
Tom van der Laan, Anne-Charlotte Dubbelman, Kevin Duisters, Alida Kindt, Amy C. Harms, Thomas Hankemeier
ANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Analytical
Tom van der Laan, Hyung Elfrink, Fatemeh Azadi-Chegeni, Anne-Charlotte Dubbelman, Amy C. Harms, Doris M. Jacobs, Ulrich Braumann, Aldrik H. Velders, John van Duynhoven, Thomas Hankemeier
Summary: In the field of metabolomics, the accurate identification of unknown compounds is crucial. A comprehensive identification workflow including LC-MS and NMR techniques was developed and successfully used to identify five taste-related features in soy sauce. Directed two-dimensional chromatography allowed for tailored fractionation of each feature, leading to the successful identification of proline-based 2,5-diketopiperazines which may contribute to taste through bitter or umami flavors.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemistry & Molecular Biology
Tom van der Laan, Isabelle Boom, Joshua Maliepaard, Anne-Charlotte Dubbelman, Amy C. Harms, Thomas Hankemeier
Article
Biochemistry & Molecular Biology
Farideh Hosseinkhani, Anne-Charlotte Dubbelman, Naama Karu, Amy C. Harms, Thomas Hankemeier
Summary: The fecal metabolome serves as a functional readout of the interactions between human metabolism and gut microbiota. The MTBE method showed superior performance compared to other extraction methods in terms of extraction efficiency and repeatability.
Article
Biochemistry & Molecular Biology
Farideh Hosseinkhani, Luojiao Huang, Anne-Charlotte Dubbelman, Faisa Guled, Amy C. Harms, Thomas Hankemeier
Summary: This study systematically evaluated the effectiveness of polar stationary phases for untargeted metabolomics using hydrophilic interaction liquid chromatography (HILIC) columns. The ZIC-c HILIC column operated at neutral pH showed superior performance for different classes of compounds, better isomer separation, repeatability and high metabolic coverage. However, the retention of inorganic ions in plasma resulted in ion-suppression as part of the overall plasma matrix effect. HILIC was successful in separating plasma samples with different phenotypes and can be used for comprehensive analysis of plasma samples and discovering metabolic changes associated with health and disease.
Article
Biochemistry & Molecular Biology
Simon Leygeber, Justus L. L. Grossmann, Carmen Diez-Simon, Naama Karu, Anne-Charlotte Dubbelman, Amy C. C. Harms, Johan A. A. Westerhuis, Doris M. M. Jacobs, Peter W. W. Lindenburg, Margriet M. W. B. Hendriks, Brenda C. H. Ammerlaan, Marco A. A. van den Berg, Rudi van Doorn, Roland Mumm, Robert D. D. Hall, Age K. K. Smilde, Thomas Hankemeier
Summary: This study evaluated the potential of a molecular-based strategy for analyzing tomato soups enhanced with yeast-derived flavor products. The combination of sensory evaluation, LC-MS, and GC-MS profiling allowed for the identification of metabolites predicting different flavors. The results highlight the advantage of combining sensory data and metabolomics platforms in exploring flavor perception in complex food matrices.
Article
Pharmacology & Pharmacy
Anne-Charlotte Dubbelman, Hilde Rosing, Robert S. Jansen, Marja Mergui-Roelvink, Alwin D. R. Huitema, Barbara Koetz, Margarita Lymboura, Larisa Reyderman, Arturo Lopez-Anaya, Jan H. M. Schellens, Jos H. Beijnen
DRUG METABOLISM AND DISPOSITION
(2012)