Article
Environmental Sciences
Jingrong He, Li Li, Yunxiang Li, Mei Huang, Ying Zhu, Shihuai Deng
Summary: In this study, six aromatic organosulfates were synthesized and their purity and concentration were determined using high performance liquid chromatography/ultraviolet detector and high performance liquid chromatography/electrospray ionization/tandem mass spectrometry. The characteristic ion fragments were found to improve the identification and detection of target compounds.
ATMOSPHERIC ENVIRONMENT
(2022)
Article
Agriculture, Multidisciplinary
Jianhua Wang, Minmin Ge, Lirui Sun, Ishfaq Ahmed, Wenjie Li, Hang Lin, Hong Lin, Zhenxing Li
Summary: A new method for the qualitative and quantitative detection of crustacean tropomyosin in various food matrices was developed, showing accuracy and reliability.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Biochemical Research Methods
Cedric E. Bobst, Justin Sperry, Olga Friese, Igor A. Kaltashov
Summary: Analytical characterization of extensively modified proteins, such as haptenated carrier proteins in synthetic vaccines, remains challenging due to structural heterogeneity. Native mass spectrometry combined with limited charge reduction overcomes these obstacles, providing meaningful characterization of heavily haptenated carrier proteins like CRM197 in smoking cessation vaccines. It reveals not only marginal conformational stability of the conjugate but also the role of haptenation extent in driving the loss of higher order structure integrity.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Chemistry, Applied
Zijian Liang, Pangzhen Zhang, Yun Xiong, Stuart K. Johnson, Zhongxiang Fang
Summary: This study characterized the phenolic and carotenoid profiles of the Australian native bulb plant, Haemodorum spicatum, using HPLC-DAD-ESI-QTOF-MS/MS and HPLC-DAD. The results identified 40 phenolic compounds and 2 carotenoids, with 35 compounds being reported for the first time in H. spicatum.
Article
Biochemical Research Methods
Mustafa Gulfen, Abdil Ozdemir, Chung-Hsuan Chen
Summary: In this study, it was observed that Ag(I) ions effectively form stable Ag(I)(1-4)-insulin complexes with high formation equilibrium constants.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Biochemical Research Methods
Judith Fischer, Mascha Treblin, Tobias Sitz, Sascha Rohn
Summary: The use of algae and cyanobacteria in human nutrition is becoming increasingly important, with health effects believed to be influenced by minor components such as sulfolipids. Studies have found compounds like sulfoquinovosyldiacylglycerols (SQDGs) to play a role in the health effects of algae and cyanobacteria.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Til Kundlacz, Carla Schmidt
Summary: Many biological processes rely on the interactions between proteins and lipids. This study systematically explores noncovalent interactions between LL-37 antimicrobial peptide and glycerophospholipids with different headgroups or fatty acyl chain lengths. The results show that ion intensities reflect hydrophobic interactions, while electrostatic interactions determine the gas-phase stability of complexes.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Maria Bikaki, Rohan Shah, Anja Mueller, Nikolai Kuhnert
Summary: The study suggests that proteins and peptides are thermally degraded through hydrolytic bond cleavage of amide bonds, forming shorter peptides as the main degradation products. The analysis revealed that hydrolytic cleavage is an important, but not dominant pathway of degradation. When heated, intact dietary proteins produce larger degradation products, while coffee globulin forms short peptides through thermal proteolysis.
Article
Environmental Sciences
Nikita Bakanov, Carolina Honert, Lisa Eichler, Gerlind U. C. Lehmann, Ralf Schulz, Carsten A. Bruehl
Summary: A simple acetonitrile-based extraction method using HPLC-ESI-MS/MS was developed for the determination of 98 current-use pesticides in soil and herbaceous vegetation. The method exhibited good accuracy and precision, with percentage recoveries ranging from 71-125% (soil) and 70-117% (vegetation), and relative standard deviations of 1-14% (soil) and 1-13% (vegetation). Matrix-matched calibration curves showed excellent linearity (R-2 > 0.99). The method was successfully applied to agricultural sites in Germany, where 44 of the 98 pesticides were detected, indicating a high qualitative load compared to EU arable soils.
Article
Biochemistry & Molecular Biology
Stefano Ercoli, Jose Parada, Luis Bustamante, Isidro Hermosin-Gutierrez, Boris Contreras, Pablo Cornejo, Antonieta Ruiz
Summary: The effect of cooking on the concentrations of phenolic compounds and antioxidant activities in 33 colored-fleshed potatoes genotypes was evaluated. It was found that anthocyanins are important contributors to the antioxidant activity of these potato genotypes, which is significant even after the drastic process of cooking.
Article
Chemistry, Applied
Christof B. Steingass, Jonas Burkhardt, Vicky Baeumer, Keshav Kumar, Heiko Mibus-Schoppe, Jana Zinkernagel, Patricia Esquivel, Victor M. Jimenez, Ralf Schweiggert
Summary: Anthocyanins were characterized in red cabbage, sweet potato, and Tradescantia pallida leaves. Various non-, mono-, and diacylated cyanidins were identified in red cabbage. Sweet potato leaves contained mono- and diacylated cyanidin- and peonidin glycosides, while T. pallida leaves mainly contained tetra-acylated anthocyanin tradescantin. The thermal stability of red cabbage and purple sweet potato extracts surpassed that of a commercial food dye, but still fell short compared to the stability of Tradescantia extract.
Article
Chemistry, Analytical
Tamara Fernandez-Bautista, Beatriz Gomez-Gomez, Roberto Palacin-Garcia, Emma Gracia-Lor, Teresa Perez-Corona, Yolanda Madrid
Summary: The distribution of Se and Hg in different protein fractions extracted from muscle tissues of tuna, swordfish, and salmon (wild vs. farmed) was investigated for the first time. The study revealed that Se and Hg were associated with proteins of specific molecular weights, with differences observed between farmed and wild salmon. Additionally, Se speciation studies showed SeMeSeCys as the primary selenium species found in the proteins analyzed.
Article
Agricultural Engineering
Shan Huang, Qingxu Ma, Qiong Hou, Ting Zuo, Zhijian Zhang, Wuzhong Ni
Summary: The bioconversion of organic waste can increase betaine content in compost, while vermicomposting tends to reduce betaine levels. Larvae bioconversion shows a more significant increase in betaines in compost.
BIORESOURCE TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Erick G. Baez Bolivar, Duong T. Bui, Elena N. Kitova, Ling Han, Ruixiang B. Zheng, Erik J. Luber, Sayed Youssef Sayed, Lara K. Mahal, John S. Klassen
Summary: This study demonstrates the facile quantification of low-affinity glycan-GBP interactions using nanoflow ESI emitters and electrospray ionization mass spectrometry (ESI-MS). The use of submicron emitters effectively eliminates nonspecific binding, enabling direct measurement of interactions with affinities as low as 5 mM. Additionally, this technique allows for the measurement of glycan affinities on specific GBPs, such as C-type lectins, and the influence of calcium ions on these interactions.
ANALYTICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Junfang Wang, Rui Zhang, Jinzhu Jiang, Wei Duan, Peige Fan, Shaohua Li, Lijun Wang
Summary: An orthogonal L(16)4(3) x 2(2) test design was used to optimize the extraction of flavan-3-ols in grape seeds. The optimized HPLC method separated and identified fourteen flavan-3-ols, showing that the new wine grape - Beihong, had higher flavan-3-ols content and polymerization compared to classic wine grapes like Cabernet Sauvignon, Merlot, Semillon, and Riesling.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Instruments & Instrumentation
Dragan Isailovic, Yang Xu, Tyler Copus, Suraj Saraswat, Surya M. Nauli
APPLIED SPECTROSCOPY
(2011)
Article
Chemistry, Physical
Suraj Saraswat, Anil Desireddy, Desheng Zheng, Lijun Guo, H. Peter Lu, Terry P. Bigioni, Dragan Isailovic
JOURNAL OF PHYSICAL CHEMISTRY C
(2011)
Article
Biochemical Research Methods
Nidhi Jaiswal, Suraj Saraswat, Manohar Ratnam, Dragan Isailovic
JOURNAL OF PROTEOME RESEARCH
(2012)
Article
Biochemistry & Molecular Biology
Wei-Cheng Tung, Bryan Rizzo, Yusef Dabbagh, Suraj Saraswat, Mark Romanczyk, Edelsys Codorniu-Hernandez, Rocio Rebollido-Rios, Paul W. Needs, Paul A. Kroon, Njara Rakotomanomana, Olivier Dangles, Karen Weikel, Joe Vinson
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2020)