Article
Geriatrics & Gerontology
Fu-Chao Liu, Mei-Ling Cheng, Chi-Jen Lo, Wen-Chuin Hsu, Gigin Lin, Huan-Tang Lin
Summary: In this study, CSF metabolomics using LC-MS was employed to investigate the metabolic changes during aging. Several metabolites were found to be significantly correlated with age, and sex differences were also observed. These findings may provide valuable insights into healthy brain aging and warrant further exploration.
Article
Chemistry, Analytical
Anran Sheng, Qingqing Chen, Mengqi Yu, Ruiqi Xiao, Tianji Zhang, Zhiyu Wang, Robert J. Linhardt, Xiaojun Sun, Lan Jin, Lianli Chi
Summary: Glycosaminoglycans (GAGs) play a role in the treatment of many human diseases, especially in thrombosis, due to their anticoagulant activity. Novel electrophoresis-mass spectrometry methods have been developed to directly elucidate the structures of larger GAG chains with more biological importance, aiding in understanding the structure-activity relationship and quality control of GAG drugs.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Sophie Catanese, Raoul K. Khanna, Antoine Lefevre, Hugo Alarcan, Pierre-Jean Pisella, Patrick Emond, Helene Blasco
Summary: To apply metabo-lipidomics in ophthalmological and systemic diseases, it is necessary to standardize the preanalytical and analytical steps in human tears. This study compared different methods for each step of the workflow and found that methanol/water extraction provided the best results for metabolomics, while Folch extraction was superior for lipidomics. The use of microcapillary glass tube for sampling was superior to Schirmer strip for metabolomics. A volume of 5 mu L provided satisfactory metabo-lipidomic coverage. These findings will contribute to improving the metabo-lipidomic workflow in human tears for the identification of new biomarkers.
Article
Chemistry, Analytical
Nikita P. Bacalzo, Garret Couture, Ye Chen, Juan J. Castillo, Katherine M. Phillips, Naomi K. Fukagawa, Carlito B. Lebrilla
Summary: A novel method for high-throughput and quantitative analysis of polysaccharides in food samples was developed, successfully quantifying nine polysaccharides with sufficient accuracy and reproducibility. Varied concentrations of polysaccharides were observed in different food samples, highlighting the potential implications for understanding the interactions of dietary polysaccharides, gut microbial community, and human health.
ANALYTICAL CHEMISTRY
(2022)
Article
Medical Laboratory Technology
Sofie K. M. van Zundert, Pieter H. Griffioen, Lenie van Rossem, Sten P. Willemsen, Yolanda B. de Rijke, Ron H. N. van Schaik, Regine P. M. Steegers-Theunissen, Mina Mirzaian
Summary: This study developed and validated a LC-MS/MS method to quantify five tryptophan metabolites and investigated their correlation with body mass index (BMI) in pregnant women. The results showed a negative correlation between BMI and the analytes TRP, 5-HTP, and 5-HIAA, and a positive correlation with KYN.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Alex Gomez-Gomez, Blanca Montero-San-Martin, Noemi Haro, Oscar J. Pozo
Summary: Evaluation of melatonin production through nail analysis shows potential for estimating global melatonin production, with correlation between nail melatonin content and age observed. Fingernails are preferred over toenails for analysis, and melatonin concentrations in nails display a rapid rise and subsequent return to baseline levels post-administration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Applied
Hyoyoung Kim, Eun Joo Baek, Yong-Kyoung Kim, Hyejin Park, Suel Hye Hur, Jang-Eok Kim, Ho Jin Kim
Summary: In this study, a specific, reliable, and suitable method for a fast and simultaneous analysis of 335 pesticides in ginseng was developed using liquid chromatography quadrupole mass spectrometry (LC-MS/MS) and gas chromatography quadrupole mass spectrometry (GC-MS/MS). The method was validated for linearity, sensitivity, selectivity, accuracy, and precision, with limits of detection (LOD) and limits of quantification (LOQ) ranging from 0.1-5.8 μg/kg and 0.3-17.5 μg/kg, respectively. Analysis of 467 ginseng samples from 2016 to 2019 revealed pesticide residues in 304 samples, but most were below the standard. The hazard quotient (HQ) of ginseng for detected pesticides was less than 1, indicating low risk.
Article
Biochemical Research Methods
Shuailong Chen, Xuan Li, Shijia Liu, Longshan Zhao, Wenyong Zhang, Zhili Xiong, Hemi Luan
Summary: A rapid, sensitive, and high-throughput isotope dilution liquid chromatography-negative ion electrospray tandem mass spectrometry (IDLC-nESI-MS/MS) method was developed for accurate quantification of multiple ceramides. This method demonstrated good linearity and recovery, and could be applied for studying ceramide biology and disease diagnosis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Yusuke Yamamoto, Toshihiro Sakurai, Zhen Chen, Takayuki Furukawa, Siddabasave Gowda B. Gowda, Yue Wu, Kazuhiro Nouso, Yuki Fujii, Yuki Yoshikawa, Hitoshi Chiba, Shu-Ping Hui
Summary: A highly sensitive LC-MS/MS method was developed to quantitatively analyze serum LysoPE species. The concentrations of LysoPEs were significantly decreased in NAFLD patients, with no significant difference between the SS and NASH groups.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Thammaporn Junsai, Saranya Poapolathep, Samak Sutjarit, Mario Giorgi, Zhaowei Zhang, Antonio Francesco Logrieco, Peiwu Li, Amnart Poapolathep
Summary: This study investigated the occurrence of multi-mycotoxins in six types of vegetable oils available in Thailand, revealing variations in mycotoxin contamination among different oil samples. The levels of analyzed mycotoxins were found to be below regulatory limits, indicating a low health risk to consumers.
Article
Biochemical Research Methods
Ryan W. Pearce, Jillian Kodger, Yana Sandlers
Summary: This study developed an LC-ESI-MS/MS method for analyzing various cellular acyl-CoA in a single injection, with a quick extraction step and compensation for matrix effects through isotopically labeled internal standards and matrix-matched calibration. The method was successfully applied to identify 21 cellular acyl-CoA in SK-HEP-1 cells and investigate alterations in acyl-CoA levels post Mito Q antioxidant intervention.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Biochemical Research Methods
L. Perez-Mayan, G. Castro, M. Ramil, R. Cela, I Rodriguez
Summary: Two different analytical methodologies were evaluated for detecting GLY and AMPA residues in wine samples, with one utilizing derivative transformation and the other direct determination. The use of molecularly imprinted sorbent for extraction and a strong anionic exchange LC column for direct determination proved to be effective in overcoming the challenges presented by the wine matrix.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Cheryl Turner, Helga Refsum, Nasser E. Bastani
Summary: In this study, an analytical method based on liquid chromatography with electrospray ionization coupled to tandem mass spectrometry detection was developed for accurate quantification of various amino acids in human plasma. The method showed excellent linearity, accuracy, precision, sensitivity, and selectivity.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Medical Laboratory Technology
Jordi Farre-Segura, Neus Fabregat-Cabello, Chiara Calaprice, Laurent Nyssen, Stephanie Peeters, Caroline Le Goff, Etienne Cavalier
Summary: A rapid and specific LC-MS/MS method for glucagon quantitation has been developed and validated, showing stability and accuracy in comparison to other immunoassay methods. This method could potentially establish a standardized approach for glucagon analysis in routine care settings.
CLINICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Fei Yang, Haozhe Cui, Chunqiong Wang, Ying Wang, Wenjing Zhu, Huimin Deng, Shanshan Liu, Zhaoyang Bian, Junli Lu, Gangling Tang, Yuan Ji
Summary: This study utilized reversed-phase liquid chromatography-tandem mass spectrometry and supercritical fluid chromatography-tandem mass spectrometry to determine the stereoisomers of chlorfenvinphos and dimethylvinphos in tobacco. A modified quick, easy, cheap, effective, rugged, and safe technique using spherical carbon was employed for the extraction and purification of tobacco samples. Both methods were compared comprehensively in terms of methods validation parameters, showing excellent linearity and adequate recoveries for the targeted stereoisomers.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Pharmacology & Pharmacy
Mohammad Al-Mandi Al-Karagholi, Jakob Moller Hansen, Dalia Abou-Kassem, Anna Koldbro Hansted, Kumari Ubhayasekera, Jonas Bergquist, Laszlo Vecsei, Inger Jansen-Olesen, Messoud Ashina
Summary: The kynurenine pathway is a potential drug discovery target in neuroscience, with L-kynurenine emerging as a prodrug targeting glutamate receptors. LKYN was found to be safe and well-tolerated in humans at various dose levels, with no significant vascular effects observed after infusion. Further exploration of LKYN's therapeutic potential in stroke and epilepsy in human studies is warranted.
PHARMACOLOGY RESEARCH & PERSPECTIVES
(2021)
Article
Veterinary Sciences
Bodil S. Holst, Sofia Carlin, Virginie Fouriez-Lablee, Sofia Hanas, Sofie Odling, Liss-Marie Langborg, S. J. Kumari A. Ubhayasekera, Jonas Bergquist, Jesper Ryden, Elin Holmroos, Kerstin Hansson
Summary: CPSE concentration is associated with future growth of the prostate in dogs, while concentrations of corticosterone and corticosteroids are positively associated with the change in relative prostatic size over time. Dogs with larger prostates at baseline are more likely to develop clinical signs of prostatic disease during follow-up.
BMC VETERINARY RESEARCH
(2021)
Article
Medicine, General & Internal
Stefan Nilsson, Angelica Wiljen, Jonas Bergquist, John Chaplin, Ensa Johnson, Katarina Karlsson, Tomas Lindroth, Anneli Schwarz, Margaretha Stenmarker, Gunilla Thunberg, Linda Esplana, Eva Frid, Malin Haglind, Angelica Hook, Joakim Wille, Joakim Ohlen
Summary: This study protocol outlines the evaluation of a digital tool, PicPecc, used by children aged 5-17 years for self-reporting symptoms of acute lymphoblastic leukaemia during high-dose methotrexate treatments. Both effect and process evaluations will be conducted, with a focus on self-reported distress, stress levels, in-app estimation of distress levels, use of analgesic medicine, and person centeredness. Research findings will be presented at international conferences and published in scientific journals.
Article
Endocrinology & Metabolism
Johanna Christina Penell, Mark M. Kushnir, Lars Lind, Jonatan Bergquist, Jonas Bergquist, P. Monica Lind, Tord Naessen
Summary: This study examined sex-specific plasma concentrations of endogenous sex and adrenal steroids in elderly Swedish Caucasians, finding that estradiol, estrone, and testosterone levels were higher in 70-year-old men compared to women. Body mass index was positively associated with estradiol and estrone in women, while negatively associated with testosterone in men. Apparently healthy individuals showed differences in hormone concentrations compared to all individuals.
ENDOCRINE CONNECTIONS
(2021)
Article
Medicine, General & Internal
Dominic Stanculescu, Lars Larsson, Jonas Bergquist
Summary: Treatment trials for prolonged critical illness and ME/CFS share significant similarities, particularly in the reactivation of endocrine axes and thyroid hormone function. These parallels may offer new avenues for treating ME/CFS.
FRONTIERS IN MEDICINE
(2021)
Review
Food Science & Technology
Sara Benede, Daniel Lozano-Ojalvo, Susana Cristobal, Joana Costa, Enza D'Auria, Tanja Cirkovic Velickovic, Maria Garrido-Arandia, Sibel Karakaya, Isabel Mafra, Gabriel Mazzucchelli, Gianluca Picariello, Alejandro Romero-Sahagun, Caterina Villa, Paola Roncada, Elena Molina
Summary: Current methods based on electrophoretic, chromatographic, or immunochemical principles have enabled the study of allergens, but there are still unknown structural features related to the allergenic potential of food proteins. It is also important to evaluate new allergens from novel protein sources that may pose new risks for consumers.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Immunology
Tobias Strid, Kazuki Okuyama, Johanna Tingvall-Gustafsson, Jacob Kuruvilla, Christina T. Jensen, Stefan Lang, Mahadesh Prasad, Rajesh Somasundaram, Josefine Ahsberg, Susana Cristobal, Shamit Soneji, Jonas Ungerback, Mikael Sigvardsson
Summary: The development of B lymphocytes is influenced by the interaction between chromatin structure and lineage-specific transcription factors. Research indicates that during B lineage commitment, essential genes have transcriptional control elements with accessible chromatin, and binding sites for key regulators like EBF1 and PAX5 are predominantly in already accessible regions. This demonstrates the interplay between the epigenetic landscape and transcription factors in cell specification.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Henrik Hill, Andris Elksnis, Per Lundkvist, Kumari Ubhayasekera, Jonas Bergquist, Bryndis Birnir, Per-Ola Carlsson, Daniel Espes
Summary: Gamma-aminobutyric acid (GABA) is an important inhibitory neurotransmitter found in the central nervous system and immune cells. Research has shown that GABA has immune-modulatory and beta-cell stimulatory effects and could potentially be used to treat type 1 diabetes. However, a study measuring the levels of GABA in T1D patients found that the levels were unchanged compared to healthy controls, suggesting that GABA secretion from beta-cells has minimal impact on systemic levels. The study also found correlations between GABA levels and certain cytokines and GAD-autoantibodies, indicating potential alterations in local GABA levels within pancreatic islets.
Article
Biochemistry & Molecular Biology
Wei Hu, Yaorui Hu, Yao Pei, Rongrong Li, Fuyi Xu, Xiaodong Chi, Jia Mi, Jonas Bergquist, Lu Lu, Luping Zhang, Chunhua Yang
Summary: A study found that there were 46 differentially expressed proteins between cervical carcinoma (CC) and normal tissues, with Integrin beta 6, PPP1CB, TMPO, PTGES3, and DTX3L significantly upregulated in CC, while Desmin was significantly downregulated. Silencing DTX3L could suppress CC cell proliferation, migration, invasion, and tumorigenesis, and enhance cell apoptosis. Furthermore, DTX3L silencing inhibited the activity of the PI3K/AKT/mTOR signaling pathway. These findings suggest that DTX3L may regulate CC progression through the PI3K/AKT/mTOR signaling pathway and could serve as a potential biomarker and therapeutic target for CC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Sofia Persson, Kumari A. Ubhayasekera, Jonas Bergquist, Sahruh Turkmen, Inger Sundstroem Poromaa, Evangelia Elenis
Summary: Polycystic ovary syndrome (PCOS) is a common endocrine disorder among women, and hyperandrogenism can cause psychological morbidity and impaired quality of life. This study aimed to investigate whether hyperandrogenism persists into midlife and its impact on quality of life.
Article
Chemistry, Analytical
Emmanuel Berlin, Veronica Lizano-Fallas, Ana Carrasco del Amor, Olatz Fresnedo, Susana Cristobal
Summary: Membrane proteins are important for understanding cellular function, but the unbiased identification of these proteins is challenging. The use of nonionic surfactants to enrich the soluble proteome can affect protein solubility and thermal stability. The introduction of nonionic surfactants is not compatible with target identification by thermal proteome profiling methodologies. Finding surfactant-free strategies for identifying membrane protein targets is the next challenge.
ANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Julio Cesar Espana Amortegui, Heidi Pekar, Mark Dennis Chico Retrato, Malin Persson, Bengt Karlson, Jonas Bergquist, Aida Zuberovic-Muratovic
Summary: A LC-MS/MS method was developed for the simultaneous identification and quantification of cyanotoxins in edible bivalves. The method successfully detected and resolved two previously detected cyanotoxin congeners separately. The method showed limitations for certain cyanotoxins but overall was specific and robust for the parameters investigated. The method was applied to analyze mussel and oyster samples collected along the Swedish coast, and the results can contribute to future regulatory monitoring for cyanotoxins to ensure seafood safety.
Article
Environmental Sciences
Veronica Lizano-Fallas, Ana Carrasco del Amor, Susana Cristobal
Summary: The exposure to multiple chemicals has raised concerns for human and environmental health. The adverse outcome pathway method provides a framework for mechanism-based assessment in environmental health, but the identification of molecular initiating events and protein targets remains a challenge. However, recent mass spectrometry-based methods offer proteome-wide identification of protein targets, but revealing a molecular initiating event is still dependent on available knowledge.
Article
Infectious Diseases
Anja Naas, Peng Li, Clas Ahlm, Elisabeth Aurelius, Josef D. Jarhult, Silvia Schliamser, Marie Studahl, Wenzhong Xiao, Jonas Bergquist, Gabriel Westman
Summary: This study investigated the temporal changes of the CSF proteome in patients with herpes simplex encephalitis (HSE) and its association with factors such as anti-NMDAR serostatus, corticosteroid treatment, brain MRI, and neurocognitive performance. The results showed that the composition and activation pathways of CSF proteins vary at different stages of the disease. Additionally, it was found that several proteins were less abundant in patients with anti-NMDAR seropositivity compared to seronegativity.
INFECTIOUS DISEASES
(2023)
Article
Chemistry, Analytical
Mark Dennis Chico Retrato, Siyuan Qiu, Anna Lundquist, Aida Zuberovic Muratovic, Farshid Mashayekhy Rad, S. J. Kumari A. Ubhayasekera, Jonas Bergquist
Summary: This study describes a simple and robust method for the identification and quantification of 22 fatty acids in TPN components using gas chromatography-mass spectrometry. The method was evaluated according to the guidelines from FDA, EMA, and ICH, and demonstrated good methodological performance. The developed method has potential applications for quality assurance and control of TPN products.
ANALYTICAL METHODS
(2023)
