☆ 4.5 Article

Determination of pseudolycorine in the bulb of lycoris radiata by capillary electrophoresis combined with online electrochemiluminescence using ultrasonic-assisted extraction

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES (2011)

期刊

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

卷 879, 期 13-14, 页码 927-932

出版社

ELSEVIER SCIENCE BV

DOI: 10.1016/j.jchromb.2011.03.002

关键词

Capillary electrophoresis; Electrochemiluminescence; Pseudolycorine; Ultrasonic extraction; Lycoris radiata

类别

Biochemical Research Methods Chemistry, Analytical

资金

National Natural Science Foundation of China [20965001, 20565001]
Guangxi Science Foundation of China [2010GXNSFA013051, 2010GXNSFF013001]

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摘要

A novel method for determination of pseudolycorine in the bulb of lycoris radiata by capillary electrophoresis coupled with online electrochemiluminescence detection with ultrasonic-assisted extraction has been developed. The effects of several factors such as detection potential, concentration and pH of phosphate buffer, separation voltage, injection time, ultrasonic power and extraction time were investigated. Under optimal conditions, the linear concentration range for pseudolycorine was 0.002-2 mu g/mL with a correlation coefficient of 0.9991. Relative standard deviations of migration time and peak areas were 1.4 and 3.2%, respectively. The limit of detection (S/N = 3) was 0.46 ng/mL. The proposed method can be successfully applied to the determination of pseudolycorine in the bulb of lycoris radiata. (C) 2011 Elsevier B.V. All rights reserved.

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