Article
Biochemistry & Molecular Biology
Shanalee C. James, Karl Fraser, Wayne Young, Phoebe E. Heenan, Richard B. Gearry, Jacqueline Keenan, Nicholas J. Talley, Susan A. Joyce, Warren C. McNabb, Nicole C. Roy
Summary: The study found that concentrations of fecal bile acids were significantly different between individuals with functional gut disorders and healthy controls, as well as between constipation and diarrhea groups. Individuals with a diarrhea phenotype had higher concentrations of bile acids compared to those with constipation.
Article
Medical Laboratory Technology
Daniel Zhi Wei Ng, Sean Xian Yu Lee, Delicia Shu Qin Ooi, Le Duc Huy Ta, Gaik Chin Yap, Carina Jing Xuan Tay, Chiung-Hui Huang, Elizabeth Huiwen Tham, Evelyn Xiu Ling Loo, Lynette P. C. Shek, Anne Goh, Hugo P. S. Van Bever, Oon Hoe Teoh, Yung Seng Lee, Fabian Yap, Kok Hian Tan, Yap-Seng Chong, Shiao Yng Chan, Johan Gunnar Eriksson, Keith M. Godfrey, Bee Wah Lee, Eric Chun Yong Chan
Summary: This study developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the high-throughput analysis of gut microbial and dietary-derived metabolites in human plasma. The method is sensitive and can quantify these metabolites within 9 minutes without prior sample derivatization, providing technical support for studying the role of these metabolites in maternal inflammation.
CLINICA CHIMICA ACTA
(2023)
Article
Immunology
Meilin Ding, Zha Zhen, Mei Ju, Suolang Quzong, Xuesi Zeng, Xiaoxia Guo, Rui Li, Mingming Xu, Jingjing Xu, Hongyang Li, Wei Zhang
Summary: Our study found significant differences in the metabolomic profiles between vitiligo patients and healthy subjects in both plateau and plain areas, with significantly higher levels of S1P in the serum of vitiligo patients and healthy subjects in the plateau area. These findings suggest that alterations in S1P metabolism may be involved in the pathogenesis of vitiligo.
CLINICAL IMMUNOLOGY
(2023)
Article
Genetics & Heredity
Serim Kim, Jong Do Seo, Yeo-Min Yun, Hanah Kim, Tae-Eun Kim, Taeheon Lee, Tae-Rim Lee, Jun Hyung Lee, Eun-Hae Cho, Chang-Seok Ki
Summary: This study investigated the pharmacokinetic properties of atorvastatin in the Korean population and its association with genetic variations. The results showed that the pharmacokinetic properties of atorvastatin in Koreans are different from those in Caucasians, and the AUC(0-24 h) of atorvastatin can be predicted based on age and eight genetic variants of ABCB1, ABCG2, APOA5, CETP, and CYP7A1.
FRONTIERS IN GENETICS
(2022)
Review
Biochemistry & Molecular Biology
Yaroslav Shansky, Julia Bespyatykh
Summary: Bile acids are important in nutrient digestion and act as signaling molecules in the blood circulation. The gut microbiota plays a role in modifying the effects of bile acids. Depending on the composition of the microbiota, bile acids can support homeostasis or contribute to pathological conditions. Bile acids have potential as biomarkers for human pathology.
Article
Biochemical Research Methods
Cheryl Turner, Helga Refsum, Nasser E. Bastani
Summary: In this study, an analytical method based on liquid chromatography with electrospray ionization coupled to tandem mass spectrometry detection was developed for accurate quantification of various amino acids in human plasma. The method showed excellent linearity, accuracy, precision, sensitivity, and selectivity.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Xiaofen Zhang, Ziyao Yang, Ziyu Shi, Zijia Zhu, Cai Li, Zhicheng Du, Yiding Zhang, Zipeng Wang, Zhenrui Jiao, Xin Tian, Ji Zhang, Wenlong Zhai, Quancheng Kan
Summary: Specific changes in plasma concentrations of BAs may serve as diagnostic biomarkers for distinguishing CCA from BBD and HC, with higher performance than CA199. A panel of 2 BA metabolites consisting of CDCA and TCDCA showed high diagnostic performance for CCA versus BBD and CCA versus HC, with higher AUC, sensitivity and specificity than carbohydrate antigen 19-9 (CA 199), a clinically employed CCA biomarker. Including samples of HCC and GC confirmed the specificity of the BA biomarkers for CCA diagnosis.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Maroula G. Kokotou, Christiana Mantzourani, Charikleia S. Batsika, Olga G. Mountanea, Ioanna Eleftheriadou, Ourania Kosta, Nikolaos Tentolouris, George Kokotos
Summary: This study presents a fast and straightforward liquid chromatography-high resolution mass spectrometry method for the simultaneous determination of 74 free fatty acids (FFAs) in human plasma. The method avoids derivatization steps and tedious sample preparation, making it suitable for the diagnosis and monitoring of metabolic disorders.
Article
Nutrition & Dietetics
Ikuya Shirai, Yu Iwasaki, Koji Karasawa, Yasutaka Shigemura, Shigeru Katayama
Summary: Agaro-oligosaccharides (AOSs) have biological activities in peripheral tissues, and AOSs with low-molecular weight can permeate the gastrointestinal tract. The systemic absorption of agarobiose is better than that of agarotetraose.
FRONTIERS IN NUTRITION
(2023)
Article
Environmental Sciences
G. Castro, M. Ramil, R. Cela, I Rodriguez
Summary: The combination of UV/free chlorine was found to significantly enhance the degradability of VALA compared to other treatments, and was more effective than UV/H2O2 at neutral pH. Three potential transformation products of VALA were identified by LC-QTOF-MS, and the formation of volatile DBPs was observed through gas chromatography.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biochemical Research Methods
Elif Ozturk Er, Belma Ozbek, Sezgin Bakirdere
Summary: A novel analytical method was described for the determination of underivatized amino acids in human plasma and urine samples, achieving accurate results through liquid chromatography-tandem mass spectrometry, demonstrating high reliability and reproducibility.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Multidisciplinary Sciences
Hyejin Park, Woo-Young Song, Hyeonjeon Cha, Tae-Young Kim
Summary: This study developed a sample preparation protocol using mass spectrometry to minimize exogenous FFA contamination during quantification in food. The optimized method includes a combination of methanol-washed glass tube and chloroform extraction solvent to minimize errors in FFA quantification.
SCIENTIFIC REPORTS
(2021)
Article
Nutrition & Dietetics
Jieping Yang, Patrick Kurnia, Susanne M. Henning, Rupo Lee, Jianjun Huang, Michael C. Garcia, Vijaya Surampudi, David Heber, Zhaoping Li
Summary: This study found that grape powder consumption significantly increased the diversity of gut microbiome, altered the abundance of specific microbial taxa, and decreased total cholesterol and HDL cholesterol levels.
Article
Obstetrics & Gynecology
Grace Huang, Shalender Bhasin, Karol Pencina, Ming Cheng, Ravi Jasuja
Summary: The study aimed to characterize the circulating androgen levels across the menstrual cycle in healthy women and report sex differences in the relative levels of dihydrotestosterone (DHT) to testosterone (T) levels. The findings showed that the total and free T levels varied significantly across the menstrual cycle in healthy premenopausal women, while the DHT levels remained stable. Additionally, women had higher DHT levels relative to total T compared to men.
FERTILITY AND STERILITY
(2022)
Article
Biochemistry & Molecular Biology
Julia Junker, Frits Kamp, Edith Winkler, Harald Steiner, Franz Bracher, Christoph Mueller
Summary: The study introduces a new method for analyzing a wide range of free neutral steroids and related substances, including intermediates of cholesterol biosynthesis, oxysterols, C19 and C21 steroids, steroid acids, and sterol sulfates. This method was validated on mouse tissue samples and cultured cells, providing detailed insight into the steroid profile in these samples.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Laura B. Ramsey, Li Gong, Seung-Been Lee, Jonathan B. Wagner, Xujia Zhou, Katrin Sangkuhl, Solomon M. Adams, Robert J. Straka, Philip E. Empey, Erin C. Boone, Teri E. Klein, Mikko Niemi, Andrea Gaedigk
Summary: PharmVar provides star allele nomenclature for the SLCO1B1 gene, which plays an important role in drug transport and metabolism. The standardized nomenclature has been adopted by CPIC and incorporated into guidelines.
CLINICAL PHARMACOLOGY & THERAPEUTICS
(2023)
Article
Pharmacology & Pharmacy
Marie-Noeurolle Paludetto, Mika Kurkela, Helina Kahma, Janne T. Backman, Mikko Niemi, Anne M. Filppula
Summary: This study aimed to explore the cytochrome P450 metabolic and inhibitory profile of hydroxychloroquine (HCQ). The results showed that CYP3A4, CYP2D6, and CYP2C8 are the key enzymes involved in HCQ metabolism. HCQ and its metabolites showed reversible inhibition of CYP2D6 and time-dependent inhibition of CYP3A. Rating: 8/10.
DRUG METABOLISM AND DISPOSITION
(2023)
Article
Pharmacology & Pharmacy
Feng Deng, Noora Sjostedt, Mariangela Santo, Mikko Neuvonen, Mikko Niemi, Heidi Kidron
Summary: Drug-drug interactions (DDIs) can have a significant impact on the safety of medication use. This study aimed to identify inhibitors of the breast cancer resistance protein (BCRP) among a library of commonly used drugs and anticancer drugs. 75 drugs were found to strongly inhibit BCRP, with vemurafenib, dabigatran etexilate, and everolimus being the most potent inhibitors. These findings suggest that clinical studies are needed to examine whether these drugs cause BCRP-mediated DDIs in humans.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Xiwei Ji, Xiangrui Meng, Qingfeng He, Xiaoqiang Xiang, Yufei Shi, Xiao Zhu
Summary: This study investigated the antitumor effects of foretinib on triple-negative breast cancer cells and its pharmacokinetic characteristics. The results showed that foretinib inhibited tumor growth in a dose-dependent manner and suppressed the expression of p-MET and HGF in both cells and tumors, suggesting that the decrease of p-MET and HGF may play a crucial role in the anti-breast cancer properties of foretinib.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Public, Environmental & Occupational Health
Jaakko Lahteenmaki, Anna-Leena Vuorinen, Mika Lehto, Mikko Niemi, Markus M. Forsberg
Summary: The study aimed to investigate the differences in healthcare costs between genetically normal and genetically sensitive warfarin responder groups. The findings showed that the sensitive responder group had higher bleeding-related hospital costs and INR test costs, but lower medication costs. This suggests that genotype-guided warfarin dosing may help reduce the risk of bleeding.
PHARMACOEPIDEMIOLOGY AND DRUG SAFETY
(2023)
Article
Pharmacology & Pharmacy
Feng Deng, Maren Laasik, Liina Salminen, Lauri Lapatto, Kaisa Huhtinen, Yilin Li, Sampsa Hautaniemi, Johanna Hynninen, Mikko Niemi, Rainer Lehtonen
Summary: The study identified genetic variations in SLCO1B3, LIG3, GSTP1, ABCB1, and OPRM1 associated with risk of adverse effects, treatment response, and prognosis in patients receiving carboplatin-paclitaxel therapy for ovarian cancer.
BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
(2023)
Article
Pharmacology & Pharmacy
Hilkka Liedes, Juha Pajula, Anna-Leena Vuorinen, Francesco De Pretis, Mark van Gils, Kari Harno, Mika Lehto, Mikko Niemi, Jaakko Lahteenmaki
BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
(2023)
Article
Pharmacology & Pharmacy
Minna Lehtisalo, Wilma Kiander, Anne M. M. Filppula, Feng Deng, Heidi Kidron, Mari Korhonen, Johanna Sinkko, Kimmo Koivula, Mikko Niemi
Summary: We report 3 cases of rhabdomyolysis diagnosed in patients 1-6 months after the concurrent use of rosuvastatin and ticagrelor. A review of the literature and the Food and Drug Administration adverse event reporting system revealed more than 40 reports of rhabdomyolysis during concomitant ticagrelor and rosuvastatin, including 3 fatal cases. In vitro studies showed that ticagrelor inhibits the transport of rosuvastatin by breast cancer resistance protein, organic anion transporting polypeptide (OATP) 1B1, 1B3, and 2B1, with inhibitory concentrations ranging from 0.36 to 7.5 μM. Our findings suggest that the concomitant use of ticagrelor and rosuvastatin may lead to an increased risk of rosuvastatin-induced rhabdomyolysis due to the inhibition of rosuvastatin transport. Further research is needed to investigate the pharmacokinetic interaction between ticagrelor and rosuvastatin in humans.
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2023)
Article
Medicine, Research & Experimental
Katja Hakkinen, Wilma Kiander, Heidi Kidron, Markku Lahteenvuo, Lea Urpa, Jonne Lintunen, Kati-Sisko Vellonen, Seppo Auriola, Minna Holm, Kaisla Lahdensuo, Olli Kampman, Erkki Isometsa, Tuula Kieseppa, Jouko Lonnqvist, Jaana Suvisaari, Jarmo Hietala, Jari Tiihonen, Aarno Palotie, Ari V. Ahola-Olli, Mikko Niemi
Summary: Six rare SLCO1B1 single nucleotide variants (SNVs) were identified in Finnish individuals with a psychotic disorder, which may affect the expression and functionality of the OATP1B1 protein. Functional studies showed that one of these SNVs (c.629G>T) resulted in the loss of OATP1B1 function and decreased membrane protein expression.
MOLECULAR PHARMACEUTICS
(2023)
Article
Medicine, Research & Experimental
Christine C. Orozco, Mikko Neuvonen, Yi-An Bi, Matthew A. Cerny, Sumathy Mathialagan, Laurie Tylaska, Brian Rago, Chester Costales, Amanda King-Ahmad, Mikko Niemi, A. David Rodrigues
Summary: This study found that sulfated bile acids (BA-S) can potentially serve as clinical biomarkers for OATP1B1/3. The BA-S substrates were observed in OATP1B1, OATP1B3, and NTCP transfected into human embryonic kidney 293 cells, with minimal uptake in other solute carriers (SLCs). Inhibition of BA-S uptake in human hepatocytes was greater with rifampicin (OATP1B1/3 selective inhibitor) than other inhibitors.
MOLECULAR PHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Feng Deng, Johanna Sistonen, Mikko Neuvonen, Mikko Niemi
Summary: This study evaluated the potential interactions between PARP inhibitors and statins, and found that PARP inhibitors can inhibit certain transporter proteins, potentially increasing the exposure of statins. However, the clinical significance of these interactions is minimal, although they may increase the risk of adverse effects when combined with other predisposing factors.
BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
(2023)
Article
Medicine, Research & Experimental
Markus Ramste, Markus Ritvos, Sergei Hayrynen, Johanna I. Kiiski, Mikko Niemi, Juha Sinisalo
Summary: The aim of this study was to investigate the influence of CYP2C19 loss-of-function variants and CYP2C19 inhibitors, such as omeprazole or esomeprazole, on the incidence of cardiovascular events in patients using clopidogrel. The study found that carriers of CYP2C19 loss-of-function alleles had a higher frequency of cardiovascular events compared to non-carriers. Additionally, the use of omeprazole or esomeprazole was similar between carriers and non-carriers. These findings suggest that both genetics and concomitant medication should be considered for optimal patient care.
CTS-CLINICAL AND TRANSLATIONAL SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
K. Ivar Lonnberg, Aleksi Tornio, Paivi Hirvensalo, Jenni Keskitalo, Anna-Liina Mustaniemi, Johanna I. Kiiski, Anne M. Filppula, Mikko Niemi
Summary: This study investigated the associations between SLCO1B1 c.521T>C and the intolerance to atorvastatin, fluvastatin, pravastatin, rosuvastatin, or simvastatin, between ABCG2 c.421C>A and the intolerance to atorvastatin, fluvastatin, or rosuvastatin, and between CYP2C9*2 and *3 alleles and the intolerance to fluvastatin. The results confirmed the association of SLCO1B1 c.521C/C genotype with simvastatin intolerance and suggested the feasibility for larger future studies.
PHARMACOGENETICS AND GENOMICS
(2023)
Article
Pharmacology & Pharmacy
Wenyu Yang, Wenyao Mak, Amanda Gwee, Meng Gu, Yue Wu, Yufei Shi, Qingfeng He, Xiaoqiang Xiang, Bing Han, Xiao Zhu
Summary: This study aimed to establish a parametric model repository of ganciclovir and valganciclovir by summarizing existing population pharmacokinetic information and analyzing the sources of variability. The results showed that dosing regimens should be adjusted according to body size and renal function.
Article
Peripheral Vascular Disease
Lotta Ulander, Piia Simonen, Heli Tolppanen, Otto Hartman, Tuomas T. Rissanen, Kari K. Eklund, Marita Kalaoja, Mika Kurkela, Mikko Neuvonen, Mikko Niemi, Janne T. Backman, Helena Gylling, Juha Sinisalo
Summary: This study evaluated the effects of hydroxychloroquine on serum and lipoprotein lipids and biomarkers of cholesterol synthesis and absorption in myocardial infarction patients treated with a high-dose statin. The results showed that hydroxychloroquine had no additional effect on cholesterol concentration or absorption, but it did interfere with lanosterol synthesis and temporarily halted the increase of the desmosterol ratio, suggesting an interference with the cholesterol synthesis pathway.
ATHEROSCLEROSIS PLUS
(2023)