Article
Chemistry, Analytical
Sophie Catanese, Raoul K. Khanna, Antoine Lefevre, Hugo Alarcan, Pierre-Jean Pisella, Patrick Emond, Helene Blasco
Summary: To apply metabo-lipidomics in ophthalmological and systemic diseases, it is necessary to standardize the preanalytical and analytical steps in human tears. This study compared different methods for each step of the workflow and found that methanol/water extraction provided the best results for metabolomics, while Folch extraction was superior for lipidomics. The use of microcapillary glass tube for sampling was superior to Schirmer strip for metabolomics. A volume of 5 mu L provided satisfactory metabo-lipidomic coverage. These findings will contribute to improving the metabo-lipidomic workflow in human tears for the identification of new biomarkers.
Article
Chemistry, Multidisciplinary
Xuezhong Zhang, Zijian Dai, Jie Chen, Xin Chen, Xiong Lin, Shuang Yang, Kai Wu, Qiang Fu, Hua Deng
Summary: Currently, the introduction of a gradient structure in moisture-electricity generators (MEGs) has significantly improved their output performance. However, it has been impossible to combine ion density and hydrophilicity gradients in one system, limiting the power and duration of MEGs. This study presents double-gradient aerogel-based MEGs prepared using carbon nanotubes and polymer dendritic colloids, which show industry-leading energy density, power density, and duration. The MEGs also have potential applications in humidity sensing, low-power device power supply, and seawater desalination, providing an inspiring approach for energy harvesting.
ENERGY & ENVIRONMENTAL SCIENCE
(2023)
Article
Microbiology
Takeo Yasu, Yuka Nomura, Yoshito Gando, Yasuhiko Matsumoto, Takashi Sugita, Nobuharu Kosugi, Masayuki Kobayashi
Summary: The study aimed to develop an ultra-simple method to measure plasma voriconazole concentration, which will help improve the implementation rate of voriconazole therapeutic drug monitoring and contribute to effective and safe use of the drug.
Article
Chemistry, Analytical
Susy Piovesana, Sara Elsa Aita, Giuseppe Cannazza, Anna Laura Capriotti, Chiara Cavaliere, Andrea Cerrato, Paolo Guarnaccia, Carmela Maria Montone, Aldo Lagana
Summary: Industrial hemp, a valuable plant with various uses, was analyzed for its fatty acid composition using an untargeted approach based on liquid-chromatography and high-resolution mass spectrometry. A total of 39 fatty acid species were tentatively identified, with five fatty acids being the most abundant across all hemp samples. Further targeted quantitative analysis revealed linolenic acid and linoleic acid as the most abundant compounds present.
Article
Chemistry, Analytical
A. Arrizabalaga-Larranaga, S. Epigmenio-Chamu, F. J. Santos, E. Moyano
Summary: This paper describes the development of a rapid and sensitive method for detecting banned dyes in spice samples using UHPLC-MS/MS technology combined with three atmospheric pressure ionization sources. The proposed method demonstrated good performance in sample analysis, with low detection limits, good precision, and accurate quantitation.
ANALYTICA CHIMICA ACTA
(2021)
Article
Engineering, Chemical
Jing Song, Bo Cheng, Yujun Wang, Jian Deng, Guangsheng Luo
Summary: In this study, an asymmetric step-T-junction was developed to enhance mass transfer by modifying the structure of the droplet formation position. The mass transfer contribution of the droplet formation stage can range from 38.1% to 48.2% for the asymmetric step-T-junction, and the mass transfer coefficient can be 27.8-64.3 times higher compared to the droplet motion stage. The mass transfer coefficient of the asymmetric step-T-junction is more than an order of magnitude higher than that of the conventional T-junction under the same residence time.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Biochemical Research Methods
Jeremy Molineau, Maria Hideux, Philippe Hennig, Sophie Bertin, Fabien Mauge, Eric Lesellier, Caroline West
Summary: A unified chromatography (UC) analysis method was developed for short-chain peptides, and compared to a reference reversed-phase ultra-high-performance liquid chromatography (RP-UHPLC) method for the analysis of 43 peptides. The two methods showed differences in orthogonality, success rate, and performance, with excellent complementarity observed in impurity profiling and separation of isomeric species.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Agricultural Engineering
Qiong Chen, Yushi Zou, Yuqian Zhu, Tianyang Guo, Yiyang Dong, Huanlu Song
Summary: This study used a non-targeted lipidomics analysis platform to determine the lipids in Eucommia ulmoides. The study found that female leaves had a higher abundance of lipids compared to male leaves, and the content of most phospholipids in both genders increased significantly after September. Differences in lipid content between seed cases and leaves were observed, and 53 phospholipids were identified with significantly different contents in the seed cases and fresh leaves samples of Eucommia ulmoides.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Chemistry, Analytical
Maria Consolacion Rodriguez-Palazon, Natalia Arroyo-Manzanares, Pilar Vinas, Natalia Campillo
Summary: This paper presents a new analytical method using DLLME and UHPLC-HRMS for the determination of capsaicin in human urine. The method showed low detection limits and good repeatability, and was successfully applied to the analysis of urine from patients treated with capsaicin-based drugs.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Applied
Suo Decheng, fan xia, Xiao Zhiming, Wei Shulin, Wang Shi, Wang Peilong
Summary: The method developed enables rapid screening and quantification of progesterone and progestins in milks using UHPLC QE HF HRMS. It meets the validation requirements for veterinary drug residue detection in the EU and China, and has been successfully applied to detect trace levels of progesterone and monitor residual progestins in real milk.
Article
Chemistry, Analytical
Yonghuan Yan, Kaoqi Lian, Haichao Zhang, Jing An, Yan Zhang, Weijun Kang, Lianfeng Ai
Summary: A rapid, sensitive, and confirmatory method was developed for the determination of 14 diuretics in multiple animal-derived foods using UHPLC-MS/MS. The method showed satisfactory recoveries and quantitation limits for surveillance monitoring of foodborne doping.
MICROCHEMICAL JOURNAL
(2022)
Article
Food Science & Technology
Yuan Gao, Mengjia Xu, Hao Wan, Chang Li, Yiqun Wan
Summary: A new method using ultra-high performance liquid chromatography was developed for the simultaneous determination of puerarin, daidzin, daidzein, and genistein in Radix puerariae. The target analytes were extracted using 70% ethylene glycol with ultrasonication, purified with N-propyl ethylenediamine (PSA) absorption, and separated on a Supersil ODS column. This method successfully determined the content of the four compounds in Radix puerariae from 11 different origins, providing fundamental data and technical means for quality control and regulation of Radix puerariae.
Article
Biochemical Research Methods
Ruben Smets, Mik van der Borght
Summary: This study characterized commercial-grade chitin and investigated a hydrolysis procedure for quantification purposes, demonstrating high accuracy and specificity. The procedure was found to be faster and less labor-intensive compared to traditional gravimetric methods.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Agriculture, Dairy & Animal Science
Marko Rudar, Alexandra Gachman, Melissa Boersma
Summary: A reproducible and robust ultra-high performance liquid chromatography method was evaluated for the simultaneous determination of cysteine, cysteinylglycine, homocysteine, gamma-glutamylcysteine, and glutathione in solid tissue. The method will facilitate novel insights into tissue-specific and whole-body sulfur amino acid metabolism in livestock animals.
JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Chemistry, Analytical
Jie Zheng, Ge-Ge Gong, Shu-Jian Zheng, Yu Zhang, Yu-Qi Feng
Summary: Carboxylic metabolites, an important class of metabolites in mammals, were successfully labeled using N-methylphenylethylamine (MPEA) to enhance detection sensitivity and chromatographic retention. The optimized method achieved high coverage analysis of carboxylated metabolites in HepG2 cells, identifying a total of 403 potential metabolites.
ANALYTICAL CHEMISTRY
(2021)
