Article
Immunology
Yun Lu, Xinxin Hu, Tongying Nie, Xinyi Yang, Congran Li, Xuefu You
Summary: Study on the membrane proteome of Acinetobacter baumannii revealed a rapid and effective preparation method, providing a more comprehensive view of changes in the membrane proteome under polymyxin B pressure.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biology
Akshay Sabnis, Katheryn L. H. Hagart, Anna Klockner, Michele Becce, Lindsay E. Evans, R. Christopher D. Furniss, Despoina A. Mavridou, Ronan Murphy, Molly M. Stevens, Jane C. Davies, Gerald J. Larrouy-Maumus, Thomas B. Clarke, Andrew M. Edwards
Summary: Colistin is a last-resort antibiotic with poor efficacy due to growing resistance. Recent research has shown that colistin exerts bactericidal activity by targeting lipopolysaccharide in the cytoplasmic membrane of bacteria. These findings offer insight into novel approaches for enhancing therapeutic outcomes.
Review
Biochemical Research Methods
Jonas Carneiro Cruz, Israel Donizeti de Souza, Fernando Mauro Lancas, Maria Eugenia Costa Queiroz
Summary: Miniaturized liquid chromatography (LC) is recognized as an important analytical method in various research fields, offering higher resolution and lower consumption. However, limitations such as tubing clogging and small sample volumes hinder high throughput and sensitive analyses. Online coupling of sample preparation techniques can overcome these limitations and improve analytical efficiency.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Pharmacology & Pharmacy
Roser Segovia, Judith Sole, Ana Maria Marques, Yolanda Cajal, Francesc Rabanal
Summary: Antibiotic resistance poses a major public health challenge, particularly with Gram-negative multidrug-resistant bacteria. Researchers have developed synthetic analogues inspired by natural polymyxins to improve activity and reduce toxicity, focusing on modifications in the hydrophobic domains. The analogues showed selective effects on anionic membranes and a mechanism of action based on the alteration of membrane function, suggesting a promising approach for the design of safer and more selective antibiotics.
Article
Biochemical Research Methods
Arjen M. Punt, Kim C. M. van der Elst, Alwin D. R. Huitema, Eef G. W. M. Lentjes
Summary: Protein precipitation solvent trichloroacetic acid (TCA), combined with stable isotope labeled internal standard, is widely used in the analysis of compounds in serum and plasma with LC-MS/MS. However, long-term use of TCA was found to have negative effects on assay performance, affecting the source of the MS. To mitigate these effects, it is recommended to use a low sample injection volume and/or shift the mobile phase to waste during TCA elution in LC-MS/MS analysis.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yuting Jin, Qiang Sun, Yumei Pei, Jing Huang
Summary: We developed a novel bioassay based on LC-MS/MS for simultaneous determination of VAN, MER, and VPA in human serum and CSF. The method was validated with satisfactory linearity, sensitivity, precision, accuracy, recovery, matrix effects, and stability. It was successfully applied to evaluate the drug levels in serum and CSF from patients with intracranial infection administered by intrathecal injection.
Article
Biochemical Research Methods
Tengfei Yuan
Summary: In this study, a sample-multiplexing LC-MS/MS method was developed and validated for the quantification of serum estrogens. The method demonstrated high accuracy and precision, and allowed simultaneous analysis of E1, E2, and E3.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Anna Roszkowska, Natalia Treder, Alina Plenis, Natalia Miekus, Ilona Oledzka, Piotr Kowalski, Tomasz Baczek
Summary: This study compared and optimized two microextraction strategies for the extraction of four immunosuppressants in biofluids. The results showed that one strategy exhibited better performance for the extraction of a novel immunosuppressive drug.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2021)
Article
Biochemistry & Molecular Biology
Elizaveta N. Fisher, Evgeny S. Melnikov, Vladimir Gegeckori, Natalya Potoldykova, Dmitry Enikeev, Kirill A. Pavlenko, Snezana Agatonovic-Kustrin, David W. Morton, Galina Ramenskaya
Summary: A novel HPLC-ESI-MS/MS method has been developed and validated for simultaneous quantitation of gonadotropin-releasing hormone (GnRH) analogs and somatostatin analogs. The method has been successfully applied to pharmacokinetic studies.
Article
Agriculture, Multidisciplinary
Myriam Bustamante-Rangel, Maria Milagros Delgado-Zamarreno, Encarnacion Rodriguez-Gonzalo
Summary: A simple and sensitive method has been developed for the analysis of anthelmintic drugs in milk, involving extraction using QuEChERS method and separation and determination by LC-MS/MS. This method provides high sensitivity for the determination of anthelmintics in milk samples and can be successfully used for routine analysis.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Chemistry, Applied
Hao Zhou, Rong Liu, Qianqian Chen, Xiantong Zheng, Jingli Qiu, Tongyan Ding, Limin He
Summary: The method based on SMISPE combined with LC-MS/MS is developed for determining residues of VCM and NVCM in milk samples, showing good linear correlation and high recoveries. It efficiently eliminates interference from matrix compounds and reduces baseline noise, making it useful for residue monitoring.
Article
Environmental Sciences
Krystyna Makowska, Julia Martin, Andrzej Rychlik, Irene Aparicio, Juan Luis Santos, Esteban Alonso, Slawomir Gonkowski
Summary: This study evaluated the exposure of dogs to parabens using fur sample analysis, finding the presence of multiple parabens in canine fur. The analysis of fur may be a useful tool for monitoring paraben exposure in dogs, suggesting a potential role in veterinary toxicology.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Simon Lagies, Roman Pichler, Georg Vladimirov, Jana Gawron, Fabian Bazner, Annabell Schreiner, Dajana Kadena, Dietmar A. Plattner, Soeren S. Lienkamp, Bernd Kammerer
Summary: Research has found that the concentration of vancomycin in the kidneys far exceeds plasma levels, potentially leading to nephrotoxic reactions. By studying animal models and cell lines, it was discovered that exposure to vancomycin resulted in metabolic and lipidomic alterations in kidney cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Lili Jin, Haibo Ding, Volkan Degirmenci, Hongchuan Xin, Qifan Miao, Qiuyu Wang, Dianbao Zhang
Summary: AWRK6, a synthesized peptide based on the natural peptide dybowskin-2CDYa, was analyzed in rat plasma using a LC-MS/MS method. Pharmacokinetic studies showed AWRK6 peaked in rat plasma at 1.2 hours after intraperitoneal injection and had a terminal half-life of approximately 2.8 hours.
Article
Environmental Sciences
Marina Bellot, Carlos Barata, Cristian Gomez-Canela
Summary: The study evaluated the stability of 17 neuroactive compounds in water and found that most compounds did not degrade, except for apomorphine and 6-hydroxydopamine which degraded completely within 24 hours. Most compounds altered motile responses to light in the crustacean Daphnia magna.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)