期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 878, 期 3-4, 页码 509-513出版社
ELSEVIER
DOI: 10.1016/j.jchromb.2009.12.032
关键词
Folates; UPLC-MS/MS; Rice analysis; Biofortification
资金
- Bijzonder Onderzoeksfonds UGent [GOA 1251204, GOA 01G00409]
- Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT Vlaanderen)
High-performance liquid chromatography, coupled to tandem mass spectrometry (HPLC-MS/MS) has been established as the method of choice for the sensitive and simultaneous determination of different folates in a particular matrix, especially when only minute quantities of material are available. Using a previously developed and validated HPLC-MS/MS method as a starting point, we here report on the development and validation of an ultra-performance liquid chromatography (UPLC-MS/MS) method for analysis of folates in rice, which allows higher throughput and better resolution. UPLC was performed under gradient conditions on an Acquity HSS T3 column, followed by tandem mass spectrometry detection. The method was validated based on linearity, sensitivity. precision. accuracy and matrix effects. The limits of detection and the lower limits of quantification varied between 0.06 and 0.45 mu g/100 g and 0.12 and 0.91 mu g/100g, respectively. Two linear calibration Curves were established, one for the low and the other for the high concentration range. Analysis of the distribution and levels of folates in wild-type and folate-biofortified rice showed up to 50-fold enrichment in biofortified rice, with total folate levels of Lip to 900 mu g/100g rice. This is the first successful implementation of a UPLC method for the rapid and sensitive quantitative determination of folates in plant material. (C) 2009 Elsevier B.V. All rights reserved.
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