Article
Biochemistry & Molecular Biology
Nguyen Ky Anh, Phamy My Tung, Min Jung Kim, Nguyen Phuoc Long, Yong-Soon Cho, Dong-Hyun Kim, Jae-Gook Shin
Summary: A method based on LC-MS/MS was developed to determine the concentration of isoniazid and its metabolites in human plasma, showing high sensitivity and stability. The method was successfully applied in TB patients and has broad applications in clinical research and treatment monitoring.
Article
Chemistry, Analytical
Oswaldo Hernandez-Gonzalez, Sergio Zarazua, Jose Ignacio Veytia-Bucheli, Marco Martin Gonzalez-Chavez, Cristian Jazmin Rodriguez-Pinal, Susanna Edith Medellin-Garibay, Edith Elena Uresti-Rivera, Francisco Javier Perez-Vazquez, Diana Patricia Portales-Perez, Silvia Romano-Moreno, Rosa del Carmen Milan-Segovia
Summary: This study developed and validated an alternative high-performance liquid chromatography method for simultaneous quantification of four antituberculosis drugs in plasma of patients. The method showed linearity, precision, and accuracy, and could be applied for therapeutic drug monitoring and pharmacokinetics studies throughout tuberculosis treatment.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Chemistry, Analytical
Holly-May Lewis, Catia Costa, Veronique Dartois, Firat Kaya, Mark Chambers, Janella de Jesus, Vladimir Palitsin, Roger Webb, Melanie J. Bailey
Summary: This study combines elemental imaging and a new method for spatially resolved lipidomics to investigate the relationship between metals, drugs, and lipids in different areas of tissues. The method was applied to rabbit lung tissues with tuberculosis infection and demonstrated the association between ion accumulation, lipid profiles, and local drug distribution.
ANALYTICAL CHEMISTRY
(2022)
Article
Infectious Diseases
Chiara Tersigni, Giulia Boiardi, Lorenzo Tofani, Elisabetta Venturini, Carlotta Montagnani, Barbara Bortone, Leila Bianchi, Elena Chiappini, Maria Iris Cassetta, Stefania Fallani, Andrea Novelli, Luisa Galli
Summary: This study evaluated serum concentrations of INH and RIF in children with TB, finding that younger children and adolescents tended to have lower plasma concentrations compared to older age groups. Monitoring drug plasma concentrations in children under 2 years of age and adolescents can help tailor treatment more effectively.
BMC INFECTIOUS DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Zhanxuan E. Wu, Marlena C. Kruger, Garth J. S. Cooper, Ivana R. Sequeira, Anne-Thea McGill, Sally D. Poppitt, Karl Fraser
Summary: Untargeted metabolomics of blood samples has been widely used to study metabolic alterations in diseases and identify biomarkers. This study found that the concentration of metabolites in plasma was more reflective of the liver profile than muscle or adipose tissue, highlighting the importance of considering the metabolomic relationship between different tissues and plasma.
Article
Chemistry, Analytical
Nicole Strittmatter, Frances M. Richards, Alan M. Race, Stephanie Ling, Daniel Sutton, Anna Nilsson, Yann Wallez, Jennifer Barnes, Gareth Maglennon, Aarthi Gopinathan, Rebecca Brais, Edmond Wong, Maria Paola Serra, James Atkinson, Aaron Smith, Joanne Wilson, Gregory Hamm, Timothy Johnson, Charles R. Dunlop, Brajesh P. Kaistha, Josephine Bunch, Owen J. Sansom, Zoltan Takats, Per E. Andren, Alan Lau, Simon T. Barry, Richard J. A. Goodwin, Duncan Jodrell
Summary: Gemcitabine distribution and metabolism were assessed using a multimodal imaging approach, revealing that active metabolites primarily reached tumor cell compartments, while an ATR inhibitor targeted nonviable tumor regions.
ANALYTICAL CHEMISTRY
(2022)
Article
Microbiology
Vanessa B. Vogensen, Mathieu S. Bolhuis, Marieke G. G. Sturkenboom, Tjip S. van der Werf, Wiel C. M. de Lange, Richard M. Anthony, Dick van Soolingen, Jan-Willem Alffenaar, Huib A. M. Kerstjens, Onno W. Akkerman
Summary: This retrospective study in a Dutch TB center investigated the impact of rifampicin on moxifloxacin exposure in patients with isoniazid-resistant or -intolerant TB. The study found a clinically significant 39% decrease in moxifloxacin exposure when rifampicin was co-administered, but moxifloxacin dose adjustment may compensate for this drug-drug interaction.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Chemistry, Analytical
Jordan L. Tanen, Samuel R. White, Duong Ha, Kaveh Jorabchi
Summary: Elemental analysis of fluorochemicals has gained attention due to increased use of fluorinated compounds. In-plasma ionization has limitations for ICPMS applications, but post-ICP chemical ionization using nanospray has improved F detection sensitivity. This study further investigates post-plasma chemical ionization and demonstrates the impact of excess plasma oxygen and use of reagent ions with different metal centers on F detection. The findings present significant improvements for elemental F analysis, especially for online chromatographic detection.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2023)
Article
Chemistry, Analytical
David Fage, Reda Brilleman, Guillaume Deprez, Marie-Christine Payen, Frederic Cotton
Summary: The development of a LC-MS/MS method for monitoring the plasma concentration of tuberculosis drugs revealed that a significant percentage of patients had subtherapeutic levels, potentially due to impaired oral absorption.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Analytical
Rory T. Steven, Marcel Niehaus, Adam J. Taylor, Ammar Nasif, Efstathios Elia, Richard J. A. Goodwin, Zoltan Takats, Josephine Bunch
Summary: Mass spectrometry imaging (MSI) is a powerful technique for providing spatially resolved atomic and molecular information from almost any sample type. Optimizing MSI methods for analysis of FFPE tissue is necessary due to the prevalence and importance of FFPE tissue storage.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Lauren M. Bishop, Oliver Fiehn
Summary: Large-scale lipidomic analyses have been limited by the cost and accessibility of traditional venipuncture sampling. Microsampling techniques offer a less-invasive and more accessible alternative. From a single drop of blood, plasma separation cards (PSC) deliver two volumetric dried plasma samples which are studied here for profiling endogenous blood lipids.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Zhidan Liang, Morgan B. Giles, Mallory J. Stenslik, Maureen Marsales, James D. Ormes, Ray Seto, Wendy Zhong
Summary: This study presents a novel application of matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) for visualizing the drug release process from non-conductive polymeric LAP implants. The researchers developed a new sample surface treatment procedure to obtain homogeneous imaging of non-conductive sample surfaces. This work is the first comprehensive report of a sample preparation methodology tailored for imaging LAP at the molecular level.
ANALYTICA CHIMICA ACTA
(2022)
Article
Medicine, General & Internal
Emily A. Kendall, Hamidah Hussain, Amber Kunkel, Rachel W. Kubiak, Anete Trajman, Richard Menzies, Paul K. Drain
Summary: The study found that using symptom-based screening for TB before initiating TPT can effectively reduce the occurrence of active TB cases, but may lead to an increase in drug resistance. Screening for subclinical TB can eliminate this increase in drug resistance, but if the screening requirement reduces access to TPT interventions, it may decrease the overall impact of TB prevention.
Article
Chemistry, Multidisciplinary
Jing Cao, Yan Xiao, Mengji Zhang, Lin Huang, Ying Wang, Wanshan Liu, Xinming Wang, Jiao Wu, Yida Huang, Ruimin Wang, Li Zhou, Lin Li, Yong Zhang, Lili Ren, Kun Qian, Jianwei Wang
Summary: Infection classification is crucial for selecting appropriate treatment plans, and early identification of causative agents is critical for disease control. Host response analysis can detect diverse and sensitive host inflammatory responses to determine the presence and type of infection. However, traditional host-derived inflammatory indicators are inadequate for clinical infection classification.
Article
Cell Biology
Qiang Su, Wei Kuang, Weiyi Hao, Jing Liang, Liang Wu, Chunmei Tang, Yali Wang, Tao Liu
Summary: Patients treated for pulmonary tuberculosis often suffer liver injury due to the effects of anti-TB drugs, which induce inflammatory responses and oxidative stress by activating the NLRP3 inflammasome. Furthermore, drug treatment reduces the activity of drug-metabolizing enzymes, altering the antioxidant defense system in liver cells.
MEDIATORS OF INFLAMMATION
(2021)