Simultaneous measurement of pazufloxacin, ciprofloxacin, and levofloxacin in human serum by high-performance liquid chromatography with fluorescence detection

In this study, three fluoroquinolones, pazufloxacin, ciprofloxacin and levofloxacin, were simultaneously determined in spiked human serum by high-performance liquid chromatography (HPLC) method with fluorescence detection Chromatography was performed using a C-8 column with an isocratic mobile phase consisting of 1% triethylamine (pH 3 0)/acetonitrile (86/14, v/v) Protein precipitation was conducted using perchloric acid and methanol The calibration curves for the three fluoroquinolones were linear over concentrations ranging from 0 1 to 20 0 mu g/mL. The within-day and between-day coefficients of variation obtained from three fluoroquinolones were less than 7%, and relative errors ranged from -1 6% to 9.3% Mean recoveries of pazufloxacin, ciprofloxacin, and levofloxacin from spiked human serum were 97%, 88%, and 90%, respectively The proposed method proved to be simple and reliable for the determination of three fluoroquinolones (C) 2010 Elsevier B V All rights reserved.