Article
Pharmacology & Pharmacy
Tianming Dai, Weifan Jiang, Min Wang, Zizheng Guo, Renke Dai
Summary: The study utilized a two-period cross-over design to investigate the bioequivalence of gefitinib in dogs. Although the mean values of the two groups were similar, they did not meet the bioequivalence criteria. ANOVA revealed a significant difference between the two periods.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Biochemical Research Methods
Johannes J. M. Rood, Javier Sastre Torano, Victor J. Somovilla, Jos H. Beijnen, Rolf W. Sparidans
Summary: The quantitation method for erlotinib and other kinase inhibitors was successfully transferred from a Thermo TSQ Quantum Ultra system to a more sensitive Sciex QTRAP5500 system, with improved sensitivity and accuracy. The new method outperformed most other published methods in terms of simplicity and speed, making it suitable for therapeutic drug monitoring.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Oncology
Chunsheng Wang, Kewei Zhao, Shanliang Hu, Wei Dong, Yan Gong, Minghuan Li, Conghua Xie
Summary: By studying the outcomes of gefitinib and erlotinib in patients with uncommon EGFR mutations in non-small cell lung cancer (NSCLC), it was found that patients with compound mutations had better treatment response, with those containing exon 19 deletion or L858R mutations showing the most significant benefits in response and survival. Additionally, the gefitinib group showed superior treatment efficacy and PFS benefit compared to the erlotinib group.
Article
Chemistry, Multidisciplinary
Chiara Fania, Marco Bagnati, Marina Albertario, Carlotta Ferraris, Marta Lamonaca, Umberto Dianzani
Summary: The analysis of immunosuppressant drugs using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is considered the gold standard. Automated systems, such as the Thermo Scientific(TM) Cascadion(TM) SM Clinical Analyzer, eliminate manual processes and provide efficient and accurate results. The comparison of LC-MS/MS with the automated system showed a good correlation between the two methods.
APPLIED SCIENCES-BASEL
(2022)
Article
Chemistry, Analytical
Linlin Dong, Nicole Bebrin, Konstantin Piatkov, Kojo Abdul-Hadi, Shinji Iwasaki, Mark G. Qian, Dong Wei
Summary: In the field of immuno-oncology, a highly sensitive and specific bioanalytical method is required for quantifying surrogate mouse antibodies, and the immunoaffinity enrichment based LC/MS/MS assay may provide an effective solution by differentiating analyte response from high endogenous immunoglobulin background.
ANALYTICAL CHEMISTRY
(2021)
Article
Toxicology
Jochem Louisse, Patrick P. J. Mulder, Arjen Gerssen, Geert Stoopen, Deborah Rijkers, Milou G. M. van de Schans, Ad A. C. M. Peijnenburg
Summary: “The study utilized a bioassay-directed analysis approach to identify potentially toxic PAs not yet included in monitoring programs. The findings suggest that this method is effective in identifying candidate PAs with genotoxic activity.”
ARCHIVES OF TOXICOLOGY
(2022)
Article
Food Science & Technology
Jing Pu, Bingye Zhao, Xin Liu, Shugang Li, Beibei Wang, Di Wu, Jinqiu Wang, Fang Geng
Summary: This study comprehensively compared the protein profiles and properties of chicken egg white and its three components. The proteomes of thick and thin egg white are similar, but thick egg white has higher abundance of ovomucin subunits and lysozymes. The properties of thick and thin egg white are significantly different. Additionally, the chalaza component has higher abundance of insoluble proteins and lower abundance of soluble proteins, which explains its insolubility. These findings are important for further research and development of egg white, such as thinning of egg white, understanding changes in egg white properties, and differential application of thick and thin egg white.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Biochemical Research Methods
Martin Meyer, Lidia Montero, Sven W. Meckelmann, Oliver J. Schmitz
Summary: This study compared the analysis of carbohydrates using four common chromatographic methods coupled to mass spectrometry. It was found that gas chromatography and reversed-phase liquid chromatography, after derivatisation, showed superior separation performance compared to the other methods. Targeted analysis using different working modes of the mass spectrometer improved sensitivity and repeatability, with RP-LC-MS in MRM mode after derivatisation being the most suitable method for monosaccharide analysis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Tengfei Yuan
Summary: Analyzing steroids in different biological matrices helps gain insight into the status of steroids.
MICROCHEMICAL JOURNAL
(2022)
Article
Plant Sciences
Hui Xu, Hang Yu, Jie Fu, Zheng-Wei Zhang, Jia-Chun Hu, Jin-Yue Lu, Xin-Yu Yang, Meng-Meng Bu, Jian-Dong Jiang, Yan Wang
Summary: This study revealed the interaction between plantamajoside and gut microbiota using high-resolution mass spectrometry and targeted metabolomics methods. The results showed that plantamajoside was rapidly metabolized into various active metabolites in the gut microbiota. Moreover, plantamajoside was found to affect short-chain fatty acid and tryptophan metabolism. These findings are significant for understanding the pharmacological effects of plantamajoside.
Article
Biochemistry & Molecular Biology
Sivamoke Dissook, Sastia Prama Putri, Eiichiro Fukusaki
Summary: Yarrowia is a model oleaginous taxon for various studies, but information on metabolite changes under different environmental conditions is limited. Nitrogen-limiting conditions have a significant impact on Yarrowia metabolism, with strains showing similar responses but unique metabolomic profiles. Purine and pyrimidine metabolism are highly affected pathways under nitrogen-limiting conditions, impacting cellular energy availability. This information is valuable for developing Yarrowia strains for scientific and industrial applications.
Article
Environmental Sciences
C. D. Metcalfe, S. Bayen, M. Desrosiers, G. Munoz, S. Sauve, V Yargeau
Summary: Endocrine disrupting chemicals (EDCs) are a diverse group of chemicals that require sensitive and varied analytical methods to monitor their presence in the environment. Only environmental matrixes with a clear link to endocrine effects are included, and emerging methods and advanced instruments for sample preparation are also discussed.
ENVIRONMENTAL RESEARCH
(2022)
Article
Immunology
Guo-ning Zhang, Ying-jia Xu, Lei Jin
Summary: This study aimed to identify differential expression of peptides associated with ankylosing spondylitis (AS) patients using LC-MS/MS analysis. Bioinformatics analysis indicated that these differentially expressed peptides might be associated with MHC class I protein binding and pathogenic Escherichia coli infection pathways, which might further affect the progression of AS. Three differentially expressed peptides were identified that could significantly promote the proliferation of fibroblasts in AS patients.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Critical Care Medicine
Shun Lu, Jianying Zhou, Hong Jian, Lin Wu, Ying Cheng, Yun Fan, Jian Fang, Gongyan Chen, Zhihong Zhang, Dongqing Lv, Liyan Jiang, Rong Wu, Xiangming Jin, Xiaodong Zhang, Junhong Zhang, Conghua Xie, Gengyun Sun, Dongning Huang, Jiuwei Cui, Renhua Guo, Zhigang Han, Zhendong Chen, Jin Liang, Wu Zhuang, Xingsheng Hu, Aimin Zang, Yi Zhang, Shundong Cang, Yuanbo Lan, Xi Chen, Laiyu Liu, Xingya Li, Jun Chen, Rui Ma, Yanzhen Guo, Ping Sun, Panwen Tian, Yueyin Pan, Zhe Liu, Peiguo Cao, Lieming Ding, Yang Wang, Xiaobin Yuan, Pengxiang Wu
Summary: This phase 3 trial compared the efficacy and safety of befotertinib with icotinib as a first-line treatment for patients with EGFR mutation-positive NSCLC. The study found that befotertinib demonstrated superior efficacy in terms of progression-free survival compared to icotinib, although it was associated with more frequent adverse events.
LANCET RESPIRATORY MEDICINE
(2023)
Article
Chemistry, Analytical
Tarek Elawady, Ahmed M. Al-Abd, Alaa Khedr, Nahed EL-Enany, Fathalla Belal
Summary: A sensitive LC-MS/MS method was developed to determine the concentration of SOR and its metabolite SNX in colorectal cancer cells. The method was able to monitor the cellular uptake and entrapment of both compounds, and confirmed the positive effect of ginger derived compounds on SOR activity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Review
Biochemistry & Molecular Biology
Marika Franczak, Isabel Toenshoff, Gerrit Jansen, Ryszard T. Smolenski, Elisa Giovannetti, Godefridus J. Peters
Summary: Mitochondria are crucial for cancer cells as they play a significant role in their energy requirements and chemoresistance. Differences in energy metabolism, such as higher NAD(+) levels, the Warburg effect, and increased one-carbon metabolism, have been identified between cancer and normal cells. Targeting these metabolic pathways could be a promising approach for developing new anticancer drugs. Combining novel drugs with conventional agents may provide effective treatments for cancer.
CURRENT MEDICINAL CHEMISTRY
(2023)
Article
Oncology
M. Houweling, U. K. Abdul, C. Brahm, T. Lagerweij, S. Heukelom, P. W. Koken, R. Honeywell, L. E. Wedekind, G. J. Peters, H. Verheul, P. Sminia, D. Noske, T. Wurdinger, B. A. Westerman
Summary: This study investigated the radio-sensitizing effect of MEK inhibitor PD0325901 in in vitro and in vivo models of glioblastoma (GBM). The results showed that both MEK inhibitors had an in vitro radio-sensitizing effect, but no significant interaction between PD0325901 MEK inhibition and irradiation was observed in in vivo experiments.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Oncology
Alessandro Gregori, Cecilia Bergonzini, Mjriam Capula, Giulia Mantini, Fatemeh Khojasteh-Leylakoohi, Annalisa Comandatore, Ghazaleh Khalili-Tanha, Alireza Khooei, Luca Morelli, Amir Avan, Erik H. Danen, Thomas Schmidt, Elisa Giovannetti
Summary: Pancreatic ductal adenocarcinoma (PDAC) is an aggressive and chemoresistant cancer, with a stiff stroma surrounding it playing a role in chemoresistance. High expression of the mechanical sensor ITGA2 correlated with a poor prognosis in PDAC patients, and increased matrix stiffness led to the expression of ITGA2 and chemoresistance to gemcitabine. ITGA2 could be a potential therapeutic target to overcome gemcitabine resistance.
Review
Biochemistry & Molecular Biology
Federica Borea, Marika A. Franczak, Maria Garcia, Matteo Perrino, Nadia Cordua, Ryszard T. Smolenski, Godefridus J. Peters, Rafal Dziadziuszko, Armando Santoro, Paolo A. Zucali, Elisa Giovannetti
Summary: Malignant Pleural Mesothelioma (MPM) is a rare neoplasm that is usually diagnosed at an advanced stage and requires systemic treatment due to its ineligibility for radical surgery. Chemotherapy has been the standard treatment for 20 years until immune checkpoint inhibitors were introduced. However, the prognosis remains poor, and targeted therapies for MPM have mostly failed in clinical trials. This review aims to explore the potential reasons for treatment failures and determine the need for further research in this area.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Calogera Claudia Spagnolo, Giuliana Ciappina, Elisa Giovannetti, Andrea Squeri, Barbara Granata, Chiara Lazzari, Giulia Pretelli, Giulia Pasello, Mariacarmela Santarpia
Summary: In recent years, the development of therapeutic agents targeting actionable oncogenic drivers in metastatic non-small cell lung cancer (NSCLC) has increased. Selective inhibitors, such as tyrosine kinase inhibitors (TKIs) and monoclonal antibodies, have shown promising results in patients with MET deregulation, specifically exon 14 skipping mutations or MET amplification. This review provides an overview of MET signaling pathways, oncogenic alterations, laboratory techniques for detection, clinical data and ongoing studies on MET inhibitors, resistance mechanisms, and potential strategies for improving outcomes in MET-exon 14-altered NSCLC patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Marika A. Franczak, Oliwia Krol, Gabriela Harasim, Agata Jedrzejewska, Nadia Zaffaroni, Carlotta Granchi, Filippo Minutolo, Amir Avan, Elisa Giovannetti, Ryszard T. Smolenski, Godefridus J. Peters
Summary: Malignant mesothelioma (MM) is a highly aggressive and resistant tumor. In this study, the cytotoxicity of new inhibitors of glucose transporter type 1 (GLUT-1) and lactate dehydrogenase-A (LDH-A) in relation to ATP/NAD+ metabolism, glycolysis and mitochondrial respiration was investigated. The inhibitors showed cytotoxicity in MM cells, associated with a decrease in ATP and NAD+, and were most effective in cells with the highest metabolic modulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Gregory J. Riely, Egbert F. Smit, Myung-Ju Ahn, Enriqueta Felip, Suresh S. Ramalingam, Anne Tsao, Melissa Johnson, Francesco Gelsomino, Raymond Esper, Ernest Nadal, Michael Offin, Mariano Provencio, Jeffrey Clarke, Maen Hussain, Gregory A. Otterson, Ibiayi Dagogo-Jack, Jonathan W. Goldman, Daniel Morgensztern, Ann Alcasid, Tiziana Usari, Paul Wissel, Keith Wilner, Nuzhat Pathan, Svitlana Tonkovyd, Bruce E. Johnson
Summary: The combination of encorafenib and binimetinib showed clinical efficacy and acceptable safety in patients with BRAF(V600E)-mutant metastatic NSCLC.
JOURNAL OF CLINICAL ONCOLOGY
(2023)
Review
Oncology
Mahrou Vahabi, Bilal Dehni, Ines Antomas, Elisa Giovannetti, Godefridus J. Peters
Summary: Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive disease with limited improvement in mortality rate despite extensive research and clinical trials. There is a need for comprehensive molecular characterization to identify biomarkers for early detection and evaluation of treatment response. MicroRNAs (miRNAs) show promise as stable biomarkers that can be detected in fixed tissues and biofluids, and their deregulation plays a role in the oncogenesis and metastasis of PDAC. Several oncomiRs and TsmiRs have been identified that contribute to chemoresistance in PDAC, and miRNA-based therapy approaches have shown success in vivo. Integrating miRNAs in PDAC treatment holds potential for personalized medicine.
CANCER AND METASTASIS REVIEWS
(2023)
Review
Biochemistry & Molecular Biology
Mahrou Vahabi, Annalisa Comandatore, Marika A. Franczak, Ryszard T. Smolenski, Godefridus J. Peters, Luca Morelli, Elisa Giovannetti
Summary: Exosomes play a crucial role in intercellular communication and can influence cancer cell behavior and response to treatment. They can transport glycolytic enzymes, leading to altered glucose metabolism and increased tumor progression, survival, immune evasion, and drug resistance. Understanding exosome-mediated cell-to-cell communication may open new therapeutic avenues and facilitate biomarker development, and combining exosome-based-targeted therapies with existing treatments holds promise in overcoming resistance and improving cancer treatment outcomes.
CYTOKINE & GROWTH FACTOR REVIEWS
(2023)
Article
Oncology
Koichi Goto, Yasushi Goto, Toshio Kubo, Kiichiro Ninomiya, Sang-We Kim, David Planchard, Myung-Ju Ahn, Egbert F. Smit, Adrianus Johannes de Langen, Maurice Perol, Elvire Pons-Tostivint, Silvia Novello, Hidetoshi Hayashi, Junichi Shimizu, Dong-Wan Kim, Chih-Hsi Kuo, James Chih-Hsin Yang, Kaline Pereira, Fu-Chih Cheng, Ayumi Taguchi, Yingkai Cheng, Wenqin Feng, Zenta Tsuchihashi, Pasi A. Janne
Summary: T-DXd demonstrates clinically meaningful responses in patients with previously treated HER2-positive non-small-cell lung cancer, with favorable safety at a dosage of 5.4 mg/kg.
JOURNAL OF CLINICAL ONCOLOGY
(2023)
Article
Oncology
Zofia Piotrowska, Daniel Shao-Weng Tan, Egbert F. Smit, Alexander I. Spira, Ross A. Soo, Danny Nguyen, Victor Ho-Fun Lee, James Chih-Hsin Yang, Vamsidhar Velcheti, John M. Wrangle, Mark A. Socinski, Marianna Koczywas, John E. Janik, Jeffrey Jones, Helena Alexandra Yu
Summary: This study demonstrates that Zipalertinib has encouraging antitumor activity in heavily pretreated patients with EGFR ex20ins-mutant NSCLC, with an acceptable safety profile and low frequency of high-grade diarrhea and rash.
JOURNAL OF CLINICAL ONCOLOGY
(2023)
Article
Chemistry, Medicinal
Daniela Carbone, Camilla Pecoraro, Giovanna Panzeca, Geng Xu, Margot S. F. Roeten, Stella Cascioferro, Elisa Giovannetti, Patrizia Diana, Barbara Parrino
Summary: A series of new nortopsentin analogs were successfully synthesized by replacing the central imidazole ring with a 1,3,4-oxadiazole or 1,3,4-thiadiazole moiety. The antiproliferative activity of these derivatives was evaluated against pancreatic cancer cell lines, showing significant reduction in cell migration. Cell cycle analysis indicated cell cycle arrest and inhibition of CDK1 activity, a crucial regulator of cell cycle progression and cancer cell proliferation.
Article
Oncology
Maryam Alaei, Seyedeh Elnaz Nazari, Ghazaleh Pourali, AliReza Asadnia, Mehrdad Moetamani-Ahmadi, Hamid Fiuji, Hamid Tanzadehpanah, Fereshteh Asgharzadeh, Fatemeh Babaei, Fatemeh Khojasteh-Leylakoohi, Ibrahim Saeed Gataa, Mohammad Ali Kiani, Gordon A. Ferns, Alfred King-yin Lam, Seyed Mahdi Hassanian, Majid Khazaei, Elisa Giovannetti, Amir Avan
Summary: In this study, the potential of targeting the enzyme cytochrome P450 (CYP450) using lopinavir/ritonavir in colorectal cancer (CRC) was explored. Experimental methods were employed to assess the effects of lopinavir/ritonavir on CRC, and it was found that inhibiting CYP450 reduced cell proliferation, induced cell death, and suppressed cell migration. Lopinavir/ritonavir also inhibited tumor growth and fibrosis. These findings suggest that targeting CYP450 with lopinavir/ritonavir has therapeutic potential in CRC and further research in this area is needed.
Article
Oncology
Alireza Asadnia, Elham Nazari, Ladan Goshayeshi, Nima Zafari, Mehrdad Moetamani-Ahmadi, Lena Goshayeshi, Haneih Azari, Ghazaleh Pourali, Ghazaleh Khalili-Tanha, Mohammad Reza Abbaszadegan, Fatemeh Khojasteh-Leylakoohi, Mohammadjavad Bazyari, Mir Salar Kahaei, Elnaz Ghorbani, Majid Khazaei, Seyed Mahdi Hassanian, Ibrahim Saeed Gataa, Mohammad Ali Kiani, Godefridus J. Peters, Gordon A. Ferns, Jyotsna Batra, Alfred King-yin Lam, Elisa Giovannetti, Amir Avan
Summary: This study identified genetic variants and differentially expressed genes in colorectal cancer patients using genome-wide DNA and RNA sequencing. ASPHD1 and ZBTB12 genes were identified as potential prognostic markers, and two novel genetic variants were found to potentially regulate gene expression. These findings provide a proof of concept for the evaluation of emerging biomarkers in colorectal cancer.