Article
Chemistry, Multidisciplinary
Tengteng Ping, Min Zheng, Pingping Zhang, Tianhao Yan, Xufeng Miao, Ke Wang, Kaoqi Lian
Summary: An analytical method using a 96-well protein precipitation plate combined with HPLC-MS/MS was developed and validated for the simultaneous determination of 12 anti-obesity drugs in human plasma. This method offers advantages of efficient and rapid analysis, simple operation, high accuracy and sensitivity. It can be used for clinically monitoring adverse reactions and guiding the appropriate use of weight-loss drugs for obese individuals.
Article
Chemistry, Analytical
Jakub Maculewicz, Piotr Stepnowski, Joanna Dolzonek, Anna Bialk-Bielinska
Summary: Imidazolium ionic liquids (ILs) are increasingly being used, and there is evidence of their presence in the environment. Due to their potential for bio-concentration, it is necessary to monitor the presence of these compounds in organisms from potentially contaminated waters. This study developed a novel analytical procedure for the determination of imidazolium ILs in mussel tissues using a combination of extraction techniques and LC-MS/MS analysis.
Article
Chemistry, Analytical
D. Luque-Cordoba, F. Priego-Capote
Summary: Steroidogenesis is a crucial process in maintaining physiological levels of steroids in the body, and its deficiency can lead to various pathologies. A research has developed a high-throughput and fully automated method for quantifying multiple steroids in human serum, achieving high sensitivity and selectivity. This method was tested on human serum samples and showed satisfactory results in line with physiological ranges reported in literature.
Article
Food Science & Technology
Yujihn Kwon, Yongeui Koo, Yoonhwa Jeong
Summary: This study developed an analytical method to determine pyrrolizidine alkaloids in teas using liquid chromatography-tandem mass spectrometry combined with rapid-easy extraction. The method showed excellent repeatability, sensitivity, and reproducibility, and could be applied to various tea samples. Overall, the method allows complete separation of lycopsamine and intermedine peaks, making it useful for monitoring pyrrolizidine alkaloids in teas.
Article
Biochemical Research Methods
Jia Zhan, Rong-rong Zhang, Xi-zhi Shi, Zhiqiang Huang, Guo-zhou Cao, Xian-feng Chen, Ling Hu
Summary: A rapid, simple, and versatile analytical method was developed for the simultaneous determination of 140 undesirable low-weight pesticides and mycotoxins in black tea. The method involved a combination of dual D-SPE with C-18 sorbent and anhydrous magnesium sulfate to eliminate interferences in the extract, maintaining the chromatographic column under ideal conditions for long-term satisfactory recoveries. Matrix-matched calibration curves were obtained for most target compounds with linear regression coefficients above 0.9900, and the limits of quantification (LOQs) ranged within 0.5-10.0 μg/kg.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Xue Tian, Sheng Yang, Qu Tang, Huoyan Ji, Lei Shen, Jianxin Wang
Summary: This paper presents a liquid chromatography-tandem mass spectrometry method for the absolute quantification of serum RBP4 with high sensitivity and specificity. The method includes a series of procedures for sample preparation and separation, followed by quantitative analysis using a mass spectrometer. The results demonstrate that the method is accurate and reliable, conforming to guidelines for bioanalytical method validation.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Daniela Maria Calucica, Costel-Valentin Manda, Amelia Maria Gaman, Stefan Raileanu, Liliana Stanca, Monica Denisa Elena Popescu, Olivia Garofita Mateescu, Andrei Bita, Octavian Croitoru, Simona-Daniela Neamtu
Summary: A simple and accurate LC-MS method was developed and validated for determining palbociclib and abemaciclib in human plasma. Solid phase extraction using Oasis PRiME HLB cartridges was used for sample preparation, providing clean extracts with a recovery extraction higher than 85%. The method can be used for quantifying the drugs in human plasma in routine analysis and proved to be useful in determining real plasma levels in cancer therapy patients.
Article
Environmental Sciences
Masho Hilawie Belay, Ulrich Precht, Peter Mortensen, Emilio Marengo, Elisa Robotti
Summary: The increasing use of pharmaceuticals and their presence in the aquatic environment have raised concerns. In this study, a new method using online solid-phase extraction (SPE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and validated for the determination of 10 pharmaceuticals in wastewaters. The method showed low limits of detection, good repeatability, and high sensitivity for ultratrace quantification of the studied pharmaceuticals in wastewaters.
Article
Chemistry, Analytical
Sara Kheirandish, Shokoufeh Goudarzi, Maryam Amirahmadi, Shiva Ghareghani, Homanaz Ghafari, Bahram Daraei, Mahmoud Ghazi-Khansari
Summary: This study aimed to detect antibiotic residues in Iranian yogurt samples using a selective multi-residues method. The results showed no antibiotic contamination in the samples.
MICROCHEMICAL JOURNAL
(2023)
Review
Thermodynamics
Sisi Xu, Yan Zheng, Cunliang Zhang, Feiyong Chen, Yang Song, Fengxia Zhang, Shuxia Zhang, Peng Liu, Lun Meng, Linxu Xu, Jin Wang, Yubo Zhao, Zhigang Yang, Xue Shen
Summary: Controlling pollution, improving sensitivity, and using high resolution mass spectrometry can determine volatile, semi-volatile, and nonvolatile PFASs.
THERMAL SCIENCE AND ENGINEERING PROGRESS
(2023)
Review
Chemistry, Analytical
Khaja Moinuddin Shaik, Bhaskar Sarmah, Gaurav Suresh Wadekar, Pramod Kumar
Summary: This review discusses the recent detection of nitrosamine impurities in pharmaceutical products, including various detection methods, mechanisms of action, sample preparation techniques, and regulatory limits.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Lorena Manzano-Sanchez, Suganthi Angappan, Jose Antonio Martinez, Ana Aguilera, Amadeo Rodriguez Fernandez-Alba
Summary: In this study, an automated micro solid-phase extraction (SPE) clean-up method was used for multi-residue analysis of pesticides in spice matrices. The method showed good recovery rates and stability, and demonstrated a 20% increase in output compared to manual clean-up.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Medicine, Legal
Vitoria Daniela Schaefer, Victoria Vendramini Muller, Lilian de Lima Feltraco Lizot, Roberta Zilles Hahn, Anelise Schneider, Marina Venzon Antunes, Rafael Linden
Summary: Hair drug testing is used for evaluating cannabis use with a long detection window and is required for granting professional driving licenses in Brazil. A sensitive LCMS/MS assay was developed and validated for quantifying THC-COOH, THC, CBD, and CBN simultaneously. The assay met validation guidelines and is ISO17025 accredited for routine testing.
FORENSIC SCIENCE INTERNATIONAL
(2021)
Article
Biochemical Research Methods
Lorena Manzano-Sanchez, Suganthi Angappan, Jose Antonio Martinez, Ana del Real Aguilera, Amadeo Rodriguez Fernandez-Alba
Summary: In this study, an automated clean-up method was used to analyze pesticide residues in various spice matrices. By using sorbent materials suitable for multiple co-extractives and an automated clean-up process, the analysis efficiency and stability were effectively improved. The results of this study demonstrate that this method can provide accurate pesticide residue results in a short amount of time.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Moo-jin Suh, Joshua B. Powers, Casey M. Daniels, Yuling Wu
Summary: This study evaluated the feasibility of a hybrid immunoaffinity capture microflow LC-MS/MS method for the analysis of antibody-drug conjugates (ADCs). The method demonstrated a wide linear range, high accuracy, and good reproducibility. It also showed a high correlation with ELISA in quantifying total antibody in plasma samples. The cost-effective microflow LC-MS method provides comprehensive information about ADCs using minimal sample volume.
