期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 877, 期 27, 页码 3169-3174出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2009.08.011
关键词
LTE4; Leukotriene; Asthma; Liquid chromatography; Mass spectrometry
资金
- NIH/NHLBI [U01 HL-81335]
- NIH [K23 ES015510-01]
- National Jewish Health GCRC and Mass Spectrometry Facility
A new analytical method suitable for high throughput measurements of LTE4 in human urine is described. The methodology utilizes on-line enrichment and liquid chromatography/tandem mass spectrometry (LC/MS/MS). The novel LC/MS/MS method is rapid, linear from 5 to 500 pg/ml in spiked urine samples of both healthy and asthmatic subjects and more accurate and precise than enzyme immunoassay (EIA) and previous LC/MS/MS methods. Results from sample integrity experiments and preliminary values of urinary LTE4 from healthy adults and children are reported. (C) 2009 Elsevier B.V. All rights reserved.
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