Article
Microbiology
Sowmyalakshmi Subramanian, Erika Mitkus, Alfred Souleimanov, Donald L. Smith
Summary: This study aimed to quantify the phytohormones involved in the initial hours after exposure of Arabidopsis thaliana to bacterial signal compounds LCO and Th17. The results showed that these compounds decreased the negative effects of drought stress on plants and increased the content of free proline after water-withhold drought stress. The study also identified changes in the levels of certain proteins related to drought stress.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemical Research Methods
Bibi Zareena, Adeeba Khadim, Syed Usama Y. Jeelani, Saddam Hussain, Arslan Ali, Syed Ghulam Musharraf
Summary: The study identified different classes of alkaloids in plant extracts using LC-MS/MS, providing valuable data for drug discovery and understanding biological pathways.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Chemistry, Analytical
Robert S. Plumb, Thomas McDonald, Paul D. Rainville, Jason Hill, Lee A. Gethings, Kelly A. Johnson, Ian D. Wilson
Summary: Vacuum jacketed column (VJC) technology can significantly improve the performance of UHPLC systems, enhancing chromatographic separation and increasing detection capabilities for metabolites. Using the VJC system can reduce peak tailing, increase peak capacity, and enhance the intensity and response of MS peaks.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Xiao-Meng Tang, Tian -Tian Ye, Xue-Jiao You, Xiao-Ming Yin, Jiang -Hui Ding, Wen-Xuan Shao, Meng -Yuan Chen, Bi-Feng Yuan, Yu-Qi Feng
Summary: In this study, a method for purifying plant ribosomal RNA (rRNA) was proposed and modifications in plant 18S rRNA and 25S rRNA were characterized using LC-ESI-MS/MS. The study revealed the presence of new modifications in plant rRNAs, including 2 modifications in 18S rRNA and 4 modifications in 25S rRNA. Exposure to cadmium induced significant changes in rRNA modifications, highlighting the importance of rRNA modifications in response to environmental stress. The discovery of new modifications expands our understanding of plant rRNA modifications and enhances the investigation of the functional roles of plant ribosomes in gene expression regulation.
CHINESE CHEMICAL LETTERS
(2023)
Article
Plant Sciences
Cyril Abadie, Julie Lalande, Guillaume Tcherkez
Summary: Plant metabolomics is widely used in plant physiology to study metabolic responses to environmental parameters. Derivatization followed by GC-MS metabolic profiling is a major technique for quantifying primary carbon, sulphur, and nitrogen metabolites. However, routine exact mass GC-MS analyses are not common due to the lack of dedicated databases. In this study, a curated database for exact mass metabolomics focused on primary metabolism was presented. The advantages of exact mass analyses were demonstrated, including resolving isotopic patterns, localizing S-containing metabolites, and avoiding identification errors. A practical example using Arabidopsis leaves showcased how exact mass GC-MS analysis can be used for plant samples and identify metabolic profiles.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Parasitology
Mingxing Zhu, Xiancai Du, Hongxia Xu, Songhao Yang, Chan Wang, Yazhou Zhu, Tingrui Zhang, Wei Zhao
Summary: Metabolomics approach revealed that the metabolism of nucleotides, alkaloids, amino acids, amides, and organic acids in mice is closely interrelated with E. granulosus infection. The study found that metabolic pathways in the liver such as tyrosine and tryptophan biosynthesis, phenylalanine, valine, leucine and isoleucine biosynthesis, and phenylalanine metabolism were notably associated with the occurrence and development of hydatid disease.
PARASITES & VECTORS
(2021)
Article
Multidisciplinary Sciences
Denise Wolrab, Robert Jirasko, Eva Cifkova, Marcus Horing, Ding Mei, Michaela Chocholouskova, Ondrej Peterka, Jakub Idkowiak, Tereza Hrnciarova, Ladislav Kuchar, Robert Ahrends, Radana Brumarova, David Friedecky, Gabriel Vivo-Truyols, Pavel Skrha, Jan Skrha, Radek Kucera, Bohuslav Melichar, Gerhard Liebisch, Ralph Burkhardt, Markus R. Wenk, Amaury Cazenave-Gassiot, Petr Karasek, Ivo Novotny, Kristina Greplova, Roman Hrstka, Michal Holcapek
Summary: Pancreatic cancer has the worst prognosis among all cancers. Cancer screening of body fluids may improve the survival time prognosis of patients. The study found significant differences in blood lipids between pancreatic cancer patients and healthy controls.
NATURE COMMUNICATIONS
(2022)
Review
Chemistry, Applied
Mariangela Valletta, Sara Ragucci, Nicola Landi, Antimo Di Maro, Paolo Vincenzo Pedone, Rosita Russo, Angela Chambery
Summary: Mass spectrometry (MS) methodologies are increasingly used in food authentication and traceability due to their unparalleled specificity, accuracy, and sensitivity. Protein and peptide profiling using MS has become more consolidated and is being utilized to assess food traceability and authenticity. Recent applications of MS in protein/peptide analyses in food matrices show potential in ensuring the quality of agro-food products.
Article
Plant Sciences
Ali Nadeem, Hira Shahzad, Bashir Ahmed, Tudor Muntean, Maaz Waseem, Aisha Tabassum
Summary: This study analyzed the chemical composition of Nepeta cataria and evaluated its antibacterial and antioxidant activities. The results showed that Nepeta cataria extracts contained a wide range of metabolites and exhibited potential antioxidant and antimicrobial properties. These findings provide a safe resource for the development of Nepeta cataria-based products.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Ivan A. Bychkov, Elena S. Pojidaeva, Anastasia S. Doroshenko, Vladimir A. Khripach, Natalia V. Kudryakova, Victor V. Kusnetsov
Summary: The coordination of activities between nuclei and organelles in plant cells is important and involves information exchange. Phytohormones play essential roles in this process. This study found that multiple hormones can cause changes in the expression of mitochondrial and nuclear genes in plant cells, which affect the cell's functions. These changes may be mediated through various pathways and genes, indicating the complex response to hormones.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Multidisciplinary
Hongyue Ma, Minli Yang, Xiujuan Wang, Bingcheng Yang, Feifang Zhang, Feng Zhang, Yinlong Li, Tong Liu, Muyi He, Qian Wang
Summary: A sulfonamide-selective ambient ion source coupled with ESI-MS was developed for selective extraction and determination of trace sulfonamide antibiotics, demonstrating the capability to directly analyze trace sulfonamide compounds in honey and pure water with recoveries ranging from 76 to 129%.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Plant Sciences
David Gomez-Zepeda, Moises Frausto, Hector-Rogelio Najera-Gonzalez, Luis Herrera-Estrella, Jose-Juan Ordaz-Ortiz
Summary: Low-molecular-weight organic acid (OA) extrusion by plant roots plays a critical role in plant nutrition, tolerance to cations toxicity, and plant-microbe interactions. This study developed two complementary methodologies for OA determination, which were used to evaluate the effect of inorganic ortho-phosphate (Pi) deficiency and aluminum toxicity on OA excretion by Arabidopsis roots. The quantification of six OAs in root exudates and the spatial location of citric and malic acid in roots and exudates were successfully achieved using targeted ultra-performance liquid chromatography coupled with high-resolution tandem mass spectrometry (UPLC-HRMS/MS) and targeted matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI).
Article
Horticulture
Anjali Rai, Khushboo Kumari, Sung Soo Han
Summary: Victoria Amazonica is a plant with ornamental, medicinal, and functional food value. Polyphenols are bioactive compounds abundant in this plant and have been studied using high-performance liquid chromatography coupled with electrospray ionization mass spectrometry. More than 108 phenolic substances were identified, of which 101 compounds have never been documented before in V. Amazonica. The plant parts showed potential utilization in the food, cosmetics, and pharmaceutical industries due to its high antioxidant activity and phenolic content.
SCIENTIA HORTICULTURAE
(2023)
Article
Plant Sciences
Anastasia Kitashova, Vladimir Brodsky, Palak Chaturvedi, Iro Pierides, Arindam Ghatak, Wolfram Weckwerth, Thomas Naegele
Summary: A plant's genome encodes the proteins necessary for metabolism, and its interactions with the environment affect its growth, development, and adaptation to adverse conditions. Despite advances in genome sequencing technologies, predicting metabolic phenotypes from genotype x environment interactions remains incomplete. Understanding the dependence and expression of molecular organization levels in growth conditions is a current challenge.
JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Biochemical Research Methods
Ibraam E. Mikhail, Masoomeh Tehranirokh, Andrew A. Gooley, Rosanne M. Guijt, Michael C. Breadmore
Summary: This review summarizes various sample clean-up and analyte enrichment strategies that can be directly coupled with ESI-MS and nano-ESI-MS for the analysis of biological fluids, including solid phase extraction, solid phase micro-extraction, slug flow micro-extraction/nano-extraction, and more.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Review
Biochemistry & Molecular Biology
Jianwu Li, Xuemin Wang
Article
Plant Sciences
Xianjun Deng, Shu Yuan, Huasheng Cao, Sin Man Lam, Guanghou Shui, Yueyun Hong, Xuemin Wang
PLANT CELL AND ENVIRONMENT
(2019)
Article
Plant Sciences
Shu Yuan, Sang-Chul Kim, Xianjun Deng, Yueyun Hong, Xuemin Wang
Article
Biochemistry & Molecular Biology
Qingwen Shen, Xinqiao Zhan, Pei Yang, Jing Li, Jie Chen, Bing Tang, Xuemin Wang, Yueyun Hong
Article
Biochemistry & Molecular Biology
Guangqin Cai, Sang-Chul Kim, Jianwu Li, Yongming Zhou, Xuemin Wang
Article
Plant Sciences
Guangqin Cai, Chuchuan Fan, Sheng Liu, Qingyong Yang, Dongxu Liu, Jian Wu, Jianwu Li, Yongming Zhou, Liang Guo, Xuemin Wang
Review
Biochemistry & Molecular Biology
Sang-Chul Kim, Xuemin Wang
Article
Multidisciplinary Sciences
Sang-Chul Kim, Liang Guo, Xuemin Wang
NATURE COMMUNICATIONS
(2020)
Article
Biotechnology & Applied Microbiology
Guangqin Cai, Geliang Wang, Sang-Chul Kim, Jianwu Li, Yongming Zhou, Xuemin Wang
Summary: The study found that increasing the expression levels of FAX1 and ABCA9 could potentially improve oil production and lipid metabolism in Camelina sativa, with their overexpression affecting seed size and lipid synthesis genes.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Multidisciplinary Sciences
Ruyi Fan, Fen Zhao, Zhou Gong, Yanke Chen, Bao Yang, Chen Zhou, Jie Zhang, Zhangmeng Du, Xuemin Wang, Ping Yin, Liang Guo, Zhu Liu
Summary: This study reports the crystal structure of Arabidopsis NPC4 and reveals that the C-terminal domain (CTD) plays a critical role in NPC4 activity. Through structure-guided biochemical studies, the authors elucidate the molecular mechanism of phospholipid hydrolysis by NPC4 and provide new insights into the phospholipase family members.
NATURE COMMUNICATIONS
(2023)
Correction
Multidisciplinary Sciences
Ruyi Fan, Fen Zhao, Zhou Gong, Yanke Chen, Bao Yang, Chen Zhou, Jie Zhang, Zhangmeng Du, Xuemin Wang, Ping Yin, Liang Guo, Zhu Liu
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Jianwu Li, Yuan Su, Charles A. Shapiro, Daniel P. Schachtman, Xuemin Wang
Summary: A study found that phosphorus deficiency increases seed oil content and alters fatty acid composition of Camelina seeds, but greatly reduces seed production. This suggests that achieving a high yield and quality of Camelina seed oil is closely linked to the phosphorus status of the soil.
Article
Cell Biology
Sang-Chul Kim, Kristen N. Edgeworth, Dmitri A. Nusinow, Xuemin Wang
Summary: This study reveals that the central clock regulators LHY and CCA1 play a role in the initial step of fatty acid synthesis in Arabidopsis. LHY enhances fatty acid synthesis, and overexpression of LHY leads to an increase in triacylglycerol accumulation in seeds.
Article
Biochemistry & Molecular Biology
Bao Yang, Maoyin Li, Anne Phillips, Long Li, Usman Ali, Qing Li, Shaoping Lu, Yueyun Hong, Xuemin Wang, Liang Guo
Summary: Phosphate is essential for plant growth, and NPC4 enzyme plays a positive role in root growth by hydrolyzing and remodeling sphingolipids during phosphate deficiency in Arabidopsis.
Article
Plant Sciences
Wenyu Li, Tengzhao Song, Lukas Wallrad, Joerg Kudla, Xuemin Wang, Wenhua Zhang
