期刊
出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2008.06.041
关键词
fatty acid methyl esters; Sinorhizobium meliloti; cyclopropane fatty acids; 3-hydroxy fatty acids; 3-hydroxybutanoic acid; poly(3-hydroxybutanoate); hydrolysis
资金
- Genome Canada through the Ontario Genomics Institute, Toronto, ON, Canada
Three protocols for fatty acid analysis in Sinorhizobium meliloti were improved by the addition of a number of standards/controls and a silylation step which allowed the determination of recoveries, extents of conversion of lipids to fatty acid methyl esters (FAMEs) and extents of side reactions. Basic hydrolysis followed by acid-catalyzed methylation and transmethylation with sodium methoxide, were the best for the analysis of 3-hydroxy- and cyclopropane fatty acids, respectively. A micro-scale, one-vial method that employed sodium methoxide/methanol was equally efficient and on a 1000-fold smaller scale than standard methods. Because this method avoids aqueous extractions, 3-hydroxybutanoic acid was detected as its trimethylsilyloxy methyl ester along with FAMEs. (C) 2008 Elsevier B.V. All rights reserved.
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