Article
Plant Sciences
Rozalia Veronika Salamon, Adriana Dabija, Agota Ferencz, Gyorgy Tanko, Marius Eduard Ciocan, Georgiana Gabriela Codina
Summary: The production of heavily hopped beers, such as Indian Pale Ale (IPA) styles, is becoming popular in Central European markets. This study investigates the dissolution rate of the main volatile component of hops, beta-myrcene, using a modified dry hopping method. The experiment demonstrates that the modified process provides a higher concentration of beta-myrcene compared to previous studies in the field.
Article
Food Science & Technology
Shi Nie, Laihao Li, Yueqi Wang, Yanyan Wu, Chunsheng Li, Shengjun Chen, Yongqiang Zhao, Di Wang, Huan Xiang, Ya Wei
Summary: This study identified and quantified volatile organic compounds (VOCs) in fermented sea bass using gas chromatography-ion mobility mass spectrometry (GC-IMS) and gas chromatography-mass spectrometry (GC-MS). Aldehydes were found to be the principal contributors to the overall flavor of fermented sea bass, and several characteristic VOCs were identified. GC-IMS was demonstrated to rapidly detect VOCs, providing a theoretical basis for studying flavor formation in fermented sea bass.
Article
Food Science & Technology
Linfen Zhao, Yanhua Wang, Dongyu Wang, Zejuan He, Jiashun Gong, Chao Tan
Summary: Headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) was used to investigate the impact of four probiotics on the volatile components of fermented coffee. The results showed that the aroma of green beans increased after fermentation, while that of roasted beans decreased. The use of probiotics in coffee fermentation can significantly enhance the aroma and improve the quality of commercial coffee beans.
Article
Microbiology
Qing Xu, Yuxiao Zhang, Xing Wang, Gejiao Wang
Summary: A soil bacterium, designated XQ2(T), isolated from Lang Mountain in Hunan province, P. R. China, is proposed as a novel species of the genus Hyphomicrobium and named Hyphomicrobium album sp. nov. based on phenotypic, chemotaxonomic and phylogenetic characteristics. The type strain is XQ2(T) (= KCTC 82378(T) = CCTCC AB 2020178(T)), and the genus description is emended.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Energy & Fuels
B. Rekha, R. Saravanathamizhan
Summary: In this study, iron oxide nanoparticles were synthesized from Spinacia oleracea by green synthesis method and sulfonated to produce an active nanocatalyst for the hydrolysis of corncob into sugar and further bioethanol production. The compositional analysis, Fourier transform infrared spectroscopy, and scanning electron microscopy were used to study the functional group and morphology changes of biomass. The effects of catalyst dosage and temperature on sugar yield were also investigated.
INTERNATIONAL JOURNAL OF ENERGY RESEARCH
(2021)
Article
Food Science & Technology
Wenyang Zhu, Bo Jiang, Fang Zhong, Jingjing Chen, Tao Zhang
Summary: This study investigated the effects of fermentation with Lactobacillus plantarum, Pediococcus pentosaceus, and Saccharomyces cerevisiae on reducing fishy odor in kelp. Results showed that Saccharomyces cerevisiae was the most effective in decreasing the volatile organic compounds associated with fishy notes, with a 91% decrease in unsaturated aldehydes. The reduction in fishy notes was related to the decrease in alpha,beta-unsaturated carbonyl groups.
Article
Green & Sustainable Science & Technology
Ramesh Doreswamy, Rajib Deb, Sachinandan De
Summary: The study successfully produced high-purity bioethanol from pig excreta and evaluated its antibacterial activity. The proper management of pig waste is crucial for public health and environmental concerns, particularly in developing countries where industrialized small-scale pig farming is on the rise. This research provides an innovative approach for pig waste management and may guide policymakers in adopting similar strategies for cleaner production and improved environmental conditions in the future.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Chemistry, Applied
Panpan Liu, Pengcheng Zheng, Lin Feng, Ziming Gong, Lin Zheng, Shiwei Gao, Xueping Wang, Fei Ye, Jianan Huang, Zhonghua Liu
Summary: The key aroma of Qingzhuan tea is aged fragrance, mainly developed during pile fermentation and enhanced during aging and drying stages. Significant contributors to the aged aroma were identified through analysis of volatile compounds, providing a theoretical basis for improving the processing and quality of Qingzhuan tea.
Article
Food Science & Technology
Tengqi Gao, Jinling Chen, Feng Xu, Yilin Wang, Pengpeng Zhao, Yunfei Ding, Yongbin Han, Jie Yang, Yang Tao
Summary: This study investigated the effects of adding mulberry leaves during the alcoholic fermentation of mulberry juice. The results showed that the addition of mulberry leaves increased the content of DNJ and quercetin in mulberry alcoholic beverages, as well as improved their antioxidant capacity. However, the total sugar, total phenols, total anthocyanins, and GABA contents decreased during fermentation.
Article
Biochemical Research Methods
Shigeaki Shibamoto, Wenjian Lu, Ayaka Sato
Summary: Multi-column gas chromatography (GC) is developed for separating analytes that cannot be separated with a single column. Traditional systems utilizing packed columns have issues such as the high frequency of valve replacements and high running costs. A heart-cutting multidimensional capillary GC using pressure switching devices has been put into practical use recently. However, it cannot be applied to the analysis of low-boiling-point gases. In this study, a silicon pneumatic microvalve and a flow channel switching module were designed to simplify method development.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Agricultural Engineering
Paolo Costa, Marina Basaglia, Sergio Casella, Christian Kennes, Lorenzo Favaro, Maria Carmen Veiga
Summary: This study demonstrates the production of PHAs from acidogenic-derived H2 and CO2 from fruit waste, offering a sustainable alternative to fossil plastics.
BIORESOURCE TECHNOLOGY
(2023)
Article
Environmental Sciences
A. M. Nizzy, S. Kannan, S. B. Anand
Summary: This study demonstrated the production of biohydrogen and organic acids from cassava industry wastewater. The results showed that the maximum hydrogen production was achieved under optimum fermentation conditions. Additionally, butyric and propionic acid were identified as the major soluble metabolites during fermentation.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Dongwei Wang, Yani Deng, Xiao Chen, Kai Wang, Lei Zhao, Zichen Wang, Xuwei Liu, Zhuoyan Hu
Summary: This study investigated the effects of three Lactobacillus Plantarum strains on the aroma profiles of pasteurized litchi juice. The results showed that pasteurization weakened the flavor profile of the juice, while LP fermentation effectively promoted flavor formation. Moreover, the combination of GC-IMS and GC-MS analysis provided a better understanding of the impact of pasteurization and fermentation on the aroma of litchi juice.
CURRENT RESEARCH IN FOOD SCIENCE
(2023)
Article
Chemistry, Analytical
David C. Bell, Randall C. Boehm, John Feldhausen, Joshua S. Heyne
Summary: This study demonstrates that spectra with relatively low coefficient of determination (R2) can come from the same chemical species, while spectra with high R2 can come from different chemical species. The R2 between spectra depends on the signal-to-noise ratio, and the residual between spectra from the same chemical species should resemble noise. The numerical characteristics of the residual between high-resolution spectra are valuable in definitively eliminating many potential matches between reference spectra and analyte sample spectra in two-dimensional gas chromatography. Additionally, numerical characteristics beyond R2 aid in ranking all potential matches logically, allowing for positive identification of a single-component analyte when reference spectra exist for all potential matches.
ANALYTICAL CHEMISTRY
(2022)
Article
Engineering, Environmental
Dongyu Ma, Jun-Xing Zhong, Xuhui Zhuang, Chao Xu, Wen Wang, Haiyang Wang, Juan Zhao, Zhiyong Yang, Yi Zhang, Zhenguo Chi
Summary: The effectiveness of post-treatment on formamide (FA)-based perovskites by applying methylammonium (MA) has not been reported. In this study, a MA-gas-releasing secondary grain growth (MGR-SGG) technique was used to stabilize FA-based perovskite and inhibit the formation of the photo-inactive 6-phase. The treatment resulted in improved crystallinity and passivation effect, leading to increased open-circuit voltages and enhanced maximum power conversion efficiency.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Energy & Fuels
Meshack I. Simeon, Felix U. Asoiro, Mohamad Aliyu, Olayinka A. Raji, Ruth Freitag
INTERNATIONAL JOURNAL OF ENERGY RESEARCH
(2020)
Article
Engineering, Biomedical
Sabine Rosenfeldt, Frank Mickoleit, Cornelia Joerke, Joachim H. Clement, Simon Markert, Valerie Jerome, Stephan Schwarzinger, Ruth Freitag, Dirk Schueler, Rene Uebe, Anna S. Schenk
Summary: This study characterizes a multi-step purification protocol for bacterial magnetosomes (MS) in terms of purity, iron content, size, and polydispersity. The potential cytotoxic effects of isolated MS when incubated with mammalian cells are also addressed. The study helps establish prerequisites for real-world applications of magnetosomes in biotechnology and biomedicine by providing a detailed overview of the process-structure relationship during the isolation of MS.
ACTA BIOMATERIALIA
(2021)
Article
Biochemistry & Molecular Biology
Michael Mader, Moritz Helm, Mingxia Lu, Martina H. Stenzel, Valerie Jerome, Ruth Freitag, Seema Agarwal, Andreas Greiner
Article
Microbiology
Nicolas Weithmann, Stanislava Mlinar, Eva Sonnleitner, Alfons Rupert Weig, Ruth Freitag
Summary: Flexible feeding of biogas production can save substrates and storage capacity while maintaining control over electricity production. Adjustment of feeding amounts enables rapid recovery of gas production levels, with a noticeable impact on microbial community dynamics.
Article
Biochemistry & Molecular Biology
Daniel Keim, Katrin Gollner, Ulrich Gollner, Valerie Jerome, Ruth Freitag
Summary: The study demonstrated that a 24-armed PDMAEMA nano-star can achieve high levels of transgene expression (40%) in human B cells while maintaining sufficient cell viability. Optimal transfection results were obtained with four days of stimulation with rhCD40L post-thawing. Furthermore, B cell subsets were found to impact transfection outcomes, highlighting the importance of considering the complexity and heterogeneity of B cell populations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
M. Helm, S. A. B. Riedl, K. Gollner, U. Gollner, V Jerome, R. Freitag
Summary: The study presents a method for isolating primary B cells from both juvenile and adult tonsillar material, achieving purities >= 80% through nylon wool column separation. This method preserves all B cell subsets and provides high number of cells from tonsils, enhancing the comparability of results in subsequent experiments. The isolated B cells demonstrate proliferative capability and show a decline in proliferative response with donor age.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Multidisciplinary Sciences
Julia Rudolph, Matthias Voelkl, Valerie Jerome, Thomas Scheibel, Ruth Freitag
Summary: The study found that macrophages can effectively ingest microplastic particles, while epithelial cells did not show significant ingestion of microplastics. Macrophages from two cell lines exhibited high ingestion of microplastics of all sizes, but cytotoxic effects were only observed in one cell line. Cell populations with high particle interactions showed signs of cellular stress and effects on cell proliferation.
SCIENTIFIC REPORTS
(2021)
Article
Ecology
Simon A. B. Riedl, Matthias Voelkl, Anja Holzinger, Julia Jasinski, Valerie Jerome, Thomas Scheibel, Heike Feldhaar, Ruth Freitag
Summary: The intestinal cells of the earthworm Eisenia fetida are suitable for studying the toxic effects of environmental pollutants on a cellular level. Exposure to pollutants and soil elutriates led to a decrease in metabolic activity of the cells. Additionally, microplastic particles did not show active uptake, but had dose and size dependent effects on metabolism that may correlate with organismic effects.
Article
Environmental Sciences
Thomas Steiner, Julia N. Moeller, Martin G. J. Loeder, Frank Hilbrig, Christian Laforsch, Ruth Freitag
Summary: High-caloric-value household biowaste is a desirable substrate for biogas and fertilizer production, but it is often contaminated by plastic packaging. This study found that fertilizer from biowaste treatment plants had significant plastic contamination, while fertilizer from agricultural biogas plants and greenery composts had minimal contamination. The level of contamination appeared to be influenced by operating conditions, with shredding of incoming material increasing the degree of contamination.
WASTE AND BIOMASS VALORIZATION
(2023)
Article
Nanoscience & Nanotechnology
Julia Jasinski, Magdalena Wilde, Matthias Voelkl, Valerie Jerome, Thomas Frohlich, Ruth Freitag, Thomas Scheibel
Summary: This study investigates the formation of protein corona on microplastic particles and its influence on particle-cell interactions. The research demonstrates that particle history plays a significant role in protein corona formation and subsequent interactions with murine intestinal epithelial-like cells. Lysozyme preincubation shows the highest rate of particle-cell interactions among the tested proteins.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Agricultural Engineering
Stanislava Mlinar, Alfons R. Weig, Ruth Freitag
Summary: Despite extensive research, the effects of NH3 and NH4+ on anaerobic digestion and associated microorganisms are still not fully understood. This study investigated the effects of different NH3/NH4+ ratios on microorganisms involved in anaerobic digestion of two substrates. It was found that bacteria, particularly gram-positive bacteria, were affected by total ammonia nitrogen (TAN), while archaea were largely influenced by NH3 concentration. Adjusting the NH3/NH4+ ratio can avoid inhibition and stabilize biogas production in nitrogen-rich substrates.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Moritz Helm, Songyan B. Huang, Katrin Gollner, Ulrich Gollner, Valerie Jerome, Ruth Freitag
Summary: Polyelectrolyte microcapsules based on SCS and PDADMAC are investigated for cultivating human primary B cells, showing that the microenvironment provided by the microcapsules accelerates the induction of B cell differentiation, particularly the formation of plasma cells, and supports their long-term survival.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Engineering, Environmental
Yuanhu Zhang, Tasmai Paul, Julian Brehm, Matthias Voelkl, Valeïrie Jerome, Ruth Freitag, Christian Laforsch, Andreas Greiner
Summary: The toxic effects of polystyrene particles are due to residual styrene monomers, which may lead to ambiguous interpretations of their impact on cells, tissues, and organisms. Therefore, it is important to analyze the residual styrene content. A simple and sensitive UV-vis method was developed to analyze residual styrene and polystyrene concentration. The study found that standard polystyrene particles containing residual monomers exhibited low but significant cytotoxicity, while in-house synthesized polystyrene particles with reduced styrene content did not.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Julia Jasinski, Matthias Voelkl, Jonas Hahn, Valerie Jerome, Ruth Freitag, Thomas Scheibel
Summary: The impact of microplastic particles on organisms is currently under intense research. This study investigated the fate of polystyrene particles ingested by murine macrophages, including their distribution during cell division and possible mechanisms of excretion. The results showed that particle distribution during cell division was cell-specific, and no active excretion of microplastic particles was observed. Additionally, polarized macrophages exhibited higher phagocytic activity and particle uptake compared to non-polarized cells. Furthermore, it was found that submicron particles were co-localized with the endoplasmic reticulum, while 0.5μm particles were occasionally found in endosomes.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Ivonne L. Diaz Ariza, Valerie Jerome, Leon D. Perez Perez, Ruth Freitag
Summary: Nonviral gene delivery vectors are less toxic and immunogenic compared to viral ones, but improvements in efficacy are still needed, with challenges in effective complexation, protection, and intracellular dissociation. Research on copolymers with multiple interactive functions has shown that optimizing transfection benefits from treatment as a multiparameter problem.
ACS APPLIED BIO MATERIALS
(2021)
