Article
Chemistry, Analytical
Lidi Gao, Hongshou Cui, Xinyu Guo, Qing Dong, Xingyu You, Xinshu Guo, Shili Qin, Lihua Jia
Summary: A zeolitic imidazolate framework (ZIF)-8-silica hybrid monolithic column with excellent reproducibility and stability was prepared. It was characterized by various techniques and showed abundant functional groups, good crystallinity, large specific surface area, and good thermal stability. When applied in capillary electrochromatography (CEC), the ZIF-8-silica hybrid monolithic column achieved baseline separation of single analytes and simultaneous enantioseparation of mixed amino acids, demonstrating its promising potential for chiral compound separation.
Review
Chemistry, Analytical
Isabela de Paula Lima, Sandy Polycarpo Valle, Marcone Augusto Leal de Oliveira, Flavia Ferreira de Carvalho Marques, Fernando Antonio Simas Vaz
Summary: Monolithic stationary phases have been widely studied in separation science, particularly in liquid chromatography and capillary electrochromatography, for over three decades. The production of monolithic columns involves various steps, including precursor solution preparation, tubing pretreatment, solution introduction, in situ reaction, and posttreatment. Different types of processes and reactions, such as sol-gel, polycondensation, free radical polymerization, and click reactions, are discussed. This work aims to provide an overview of the production process and the characteristics of monolith compositions, as well as to encourage researchers in finding suitable monoliths for analytical separations.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Yu Xu, Ang Li, Song Xue, Sihui Ding, Qi Zhang
Summary: This study demonstrates the use of hydrophobic deep eutectic solvents (HDESs) in capillary electrophoresis (CE) for chiral separations. The HDES N8881Cl:OctA can form nano-sized microdroplets in CE background electrolyte solutions and acts as a new type of pseudo-stationary phase (PSP) exhibiting hydrophobic effects. The presence of N8881Cl:OctA significantly improves the enantioresolutions of model drugs with various cyclodextrin (CD)-type chiral selectors, and the migration time of enantiomers can be reduced using an anionic CD.
Article
Biochemical Research Methods
Ali Derazshamshir, Ilgim Gokturk, Fatma Yilmaz, Adil Denizli
Summary: This study successfully achieved the selective separation of the enantiomeric form of R,S-citalopram, S-CIT, using the molecular imprinting method. The design of an amino acid-based molecularly imprinted monolithic capillary column demonstrated high imprinting efficiency and selectivity for S-CIT.
Article
Biochemical Research Methods
Pandeng Miao, Liu Zhang, Jian Zhang, Mingxuan Ma, Yingxiang Du, Jie Gan, Jiangxia Yang
Summary: Metal-organic frameworks (MOFs) are microporous hybrid materials formed by coordination of metal cations with organic ligands. In this study, MOF-5 was grown on a polymer support and bonded with pepsin for enantioseparation of chiral drugs.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Chemistry, Physical
Ang Li, Siru Ren, Chuanjiang Teng, Hongmei Liu, Qi Zhang
Summary: Deep eutectic solvents (DESs) have potential applications as additives/modifiers in chiral electrokinetic chromatography (EKC) and can moderately improve the enantioseparation performance. The improvement is mainly attributed to the contribution of hydrogen-bond acceptors (HBAs) rather than hydrogen-bond donors (HBDs). However, using chiral DESs does not show obvious advantages compared to achiral DESs, which is different from the performance of chiral ionic liquids (ILs) as additives in chiral EKC.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Pharmacology & Pharmacy
Yikun Liu, Ning He, Yingfang Lu, Weiqiang Li, Xin He, Zhentao Li, Zilin Chen
Summary: In this study, a styrene-based polymer monolithic column poly(VBS-co-TAT-co-AHM) with reversed-phase/hydrophilic interaction liquid chromatography (RPLC/HILIC) bifunctional separation mode was successfully prepared for capillary electrochromatography. The incorporation of VBS functional monomer enabled the monolith to achieve efficient separation of various compounds in RPLC or HILIC mode. The prepared poly(VBS-co-TAT-co-AHM) monolith exhibited excellent reproducibility and stability, making it highly valuable and promising for capillary electrochromatography (CEC).
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2023)
Article
Chemistry, Multidisciplinary
Feijie Liu, Zhimin Xue, Xue Lan, Zhenghui Liu, Tiancheng Mu
Summary: The concept of CO2 switchable deep eutectic solvents has been introduced with a series of CO2 switchable imidazole-based deep eutectic solvents designed. Research on the CO2 switch mechanism and its application in easy emulsion separation of olive oil from DESs have shown great potential in dissolution and easy separation.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Ang Li, Song Xue, Yu Xu, Sihui Ding, Di Wen, Qi Zhang
Summary: The study explores the feasibility of using hydrophobic eutectic solvents (HESs) as pseudo-stationary phases (PSPs) in capillary electrophoresis (CE) for chiral separations. It demonstrates that there is a potential synergistic effect between HESs and traditional chiral selectors, improving enantioseparations of basic drugs.
ANALYTICA CHIMICA ACTA
(2023)
Article
Engineering, Chemical
Ke Li, Kai Zong, Ziyue Zhou, Dongshun Deng
Summary: This study conducted new lithium deep eutectic solvents (DESs) by pairing simple lithium salt with various hydrogen bond donors, with LiCl/EG (1:3) DES showing exceptional performance in NH3 capture. The results provide useful insights for designing metallic DESs for NH3 separation applications.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Iwona Cichowska-Kopczynska, Bartosz Nowosielski, Dorota Warminska
Summary: Nowadays, researchers are striving to find a solution to the problem of global warming, with a focus on reducing carbon dioxide emissions. The use of alkanolamines for carbon dioxide capture is problematic due to its corrosive nature, volatility, and high energy demand. Deep eutectic solvents (DESs) have emerged as a potential alternative, capable of physically and chemically absorbing carbon dioxide. Different strategies, such as the addition of water or co-solvents, have been proposed to enhance CO2 absorption using DESs. This review presents an overview of DESs, their impact on CO2 absorption, and practical considerations for their use in CO2 separation.
Review
Chemistry, Analytical
Amir M. Ramezani, Raheleh Ahmadi, Yadollah Yamini
Summary: Deep eutectic solvents (DESs) have gained significant attention as an environmentally friendly alternative to ionic liquids and organic solvents in various fields. This paper provides a brief overview of the history, properties, and synthesis procedure of DESs, with a focus on the review of homogenous liquid-liquid microextraction methods (HLLME) based on DESs.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Luana Bastos Santos, Rosivan Santos Assis, Jeferson Alves Barreto, Marcos Almeida Bezerra, Cleber Galvao Novaes, Valfredo Azevedo Lemos
Summary: In recent years, there has been a significant increase in the search for green chemistry principles in analytical methods. Solvents play a crucial role in green analytical chemistry, and certain criteria must be met for a solvent to be considered green, including availability, low toxicity, biodegradability, and low cost. However, the availability of green solvents is currently limited. Deep eutectic solvents (DESs) have emerged as a new and promising alternative to traditional solvents, attracting attention for their eco-friendly, inexpensive, and easily obtainable characteristics. This work provides a critical review of the use of DESs in liquid-phase microextraction (LPME) and their contributions to green chemistry.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Aditi Prabhune, Ranjan Dey
Summary: Deep Eutectic Solvents (DESs) have emerged as potential alternatives to conventional solvents in many industrial applications due to their safety, greenness, ease of preparation and wide range of applications.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Engineering, Chemical
Qian Liu, Xianglan Zhang
Summary: This study proposes a systematic method for screening promising deep eutectic solvents (DESs) as extractive solvents, and verifies the reliability of the conductor-like screening model for real solvents (COSMO-RS) using experimental data. The research identifies chlorocholine chloride (CChC): acetic acid (AA) and choline chloride (ChCl): ethylene glycol (EG) as the optimal DESs for the separation of m-cresol/cumene. Extraction experiments confirm the high separation performance of these DESs.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)