An artificial receptor fabricated by target recognition determinant imprinting for selective capture of α-amanitin

This is the first reported work of artificial alpha-amanitin receptors which are prepared using the design and synthesis of a template molecule based on an alpha-amanitin recognition determinant imprinting strategy. The resultant molecularly imprinted polymers (MIPs) are evaluated using high performance liquid chromatography (HPLC), binding experiment, nitrogen adsorption measurement, and solid-phase extraction. Experiment clearly demonstrates that the MIPs as the HPLC stationary phase can specifically recognize alpha-amanitin from analogues. The MIPs also successfully adsorb trace amounts of alpha-amanitin in spiked serum samples selectively pretreated and enriched through molecularly imprinted solid phase extraction. The limit of detection and recovery is 3.0 ng mL(-1) and 88.5-95.9%, respectively, for alpha-amanitin in serum samples. The high specific adsorption and excellent selectivity of the MIPs arises from imprinting effects related to the imprinting cavity of the polymeric matrix, the metal-coordination bond, and the hydrogen bond between the receptor and ligand. (C) 2013 Elsevier B.V. All rights reserved.