期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1369, 期 -, 页码 105-115出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2014.10.010
关键词
Reversed phase liquid chromatography; Glycerolipids; Phospholipids; Evaporative light scattering detector; Charged aerosol detector; Mass spectrometry
资金
- Lipidor AB, Sweden [201001]
This paper reports a simple chromatographic system to separate lipids classes as well as their molecular species. By the use of phenyl coated silica as stationary phase in combination with a simple mobile phase consisting of methanol and water, all tested lipid classes elute within 30min. Furthermore, a method to accurately predict retention times of specific lipid components for this type of chromatography is presented. Common detection systems were used, namely evaporative light scattering detection (ELSD), charged aerosol detection (CAD), electrospray mass spectrometry (ESI-MS), and UV detection. (C) 2014 Elsevier B.V. All rights reserved.
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