Article
Chemistry, Analytical
Petra Sazelova, Veronika Solinova, Tereza Schimperkova, Jiri Jiracek, Vaclav Kasicka
Summary: Chiral CE methods were used to separate and identify the configuration of the tyrosine residue in β-Ala-Tyr, and to evaluate the enantiopurity of its synthetic isomers and derivatives. The results showed that the isolated β-Ala-Tyr was pure L-enantiomer.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Chemistry, Analytical
Jumi Kim, Hyunwoo Kim
Summary: In this study, a comprehensive chiral analysis method based on F-19 NMR spectroscopy was introduced for accurate differentiation and analysis of monosaccharides. The method utilizes sugar hydrazones with fluorine labels and chiral metal complexes.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Lixia Chen, Xiangjun Li, Tingting Tian, Sen Yang, Huiqun Wang
Summary: In this study, a simple and efficient method for separating baclofen enantiomers by capillary electrophoresis was established. Molecular modeling and density functional theory were used to simulate and investigate the chiral resolution mechanism of electrophoresis, and the calculated intermolecular forces were directly presented by visualization software. Furthermore, the theoretical and experimental electronic circular dichroism (ECD) spectra of ionized baclofen were compared to determine the configuration of the dominant enantiomer in the nonracemic mixture.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Engineering, Chemical
Junwei Zhang, Zhen Sun, Jianjun Chen
Summary: A two-dimensional transport dispersive model based on the Navier-Stokes equation and a one-dimensional transport dispersive model were built to describe the diffusions of glycolic acid (GA) and NaCl in a preparative column. The models successfully simulated the elution and breakthrough curves of GA and NaCl, and the concentration profiles in the column. The results indicated that the external transfer diffusion was the rate-controlling step for GA adsorption, and the concentration of NaCl was slightly higher than that of GA. Increasing the column temperature led to a decrease in the retention time of GA and its resolution, while the diffusion of NaCl remained relatively unchanged.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2023)
Article
Engineering, Chemical
Qi Zhang, Siru Ren, Song Xue, Ang Li, Siyao Liu, Xiaodong Sun
Summary: The study evaluated six novel ionic liquids as chiral selectors in capillary electrophoresis, showing better enantioseparation performance compared to traditional chiral selectors.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Engineering, Chemical
Qi Zhang, Siru Ren, Ang Li, Jing Zhang, Song Xue, Xiaodong Sun
Summary: This study designed and successfully synthesized six tartaric acid-based IL-type chiral selectors, which demonstrated good enantioseparation performance in chiral capillary electrophoresis. Among them, [BMPIP](2)-L-TT showed the best performance, indicating the significant role of cations in enantiorecognition process.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Chemistry, Inorganic & Nuclear
Chengfeng Zhu, A-Mei Zhang, Ying Li, Han-Xue Li, Yijian Qian, Yanming Fu, Xiang Wu, Yougui Li
Summary: A chiral metal-organic framework (CMOF) with accessible binding pockets and multiple recognition sites was constructed using a tricarboxylate ligand derived from phenylalanine. The CMOF has a rare structure with cavities encapsulated by a non-coordinated amino-acid moiety. It has been proven to be an efficient and recyclable chiral host, capable of adsorbing and separating racemic alcohols, diols, and epoxides with excellent enantioselectivity.
INORGANIC CHEMISTRY FRONTIERS
(2022)
Article
Biochemical Research Methods
Joanie Otin, N. Thuy Tran, Aurelie Benoit, Corinne Buisson, Myriam Taverna
Summary: This study proposes a capillary electrophoresis method to analyze misused growth hormone-releasing hormone (GHRH) analogs. The use of dimethyl-beta-cyclodextrin as a chiral selector allows the separation of basic peptide analogs, including enantiopeptides that differ by one amino acid chirality. Electrokinetic preconcentration methods were investigated to increase sensitivity. The combination of preconcentration with chiral capillary zone electrophoresis successfully separates GHRH analogs with detection limits ranging from 75 to 200 ng/mL.
Article
Chemistry, Physical
Xiaoxiao Li, Xuefeng Tong, Qibin Chen, Honglai Liu
Summary: The size of graphene oxide sheets plays a crucial role in the enantioselectivity of membrane separation. Larger lateral size of GO sheets can result in better chiral separation performance. Functionalizing GO sheets with chiral selectors can enhance the enantiomer permeability and selectivity.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2021)
Article
Chemistry, Analytical
Jan Bilek, Dusan Koval, Petra Sazelova, Veronika Solinova, Lukas Severa, Paul E. Reyes Gutierrez, Filip Teply, Vaclav Kasicka
Summary: A chiral capillary electrophoresis (CE) method has been developed to separate the P- and M-enantiomers of 11 new diquats, achieving fast and efficient separations within 5-7 minutes. The method utilizes acidic buffer and sulfated cyclodextrins as chiral selectors, with sulfated γ-cyclodextrin showing the best selectivity by baseline separating all 11 diquats.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Physical
Bhavya Mishra, Margaret E. Johnson
Summary: This study investigates how proteins exploit the reduced dimensionality of membranes to trigger complex formation, finding that proteins can accelerate dimer formation due to an increase in relative concentration. By identifying major rate-limiting pathways, an excellent approximation for the mean first passage time is derived, highlighting the central role of the sticking rate or effective adsorption coefficient of the membrane in controlling timescales.
JOURNAL OF CHEMICAL PHYSICS
(2021)
Article
Chemistry, Physical
Xiaofei Ma, Jing Cao, Juan Yu, Liangliang Cai
Summary: In this study, an ionic liquid chiral selector based on sulfobutylether-8-cyclodextrin (SBE-8-CD) was prepared for enantioseparation in capillary electrophoresis (CE) for the first time. The ionic liquid exhibited superior enantioselectivity compared to native SBE-8-CD, without obvious prolongation of migration time. Factors affecting the enantioseparations were investigated, and the method was successfully used for determining the enantiomeric purity of amlodipine besylate (AML). The chiral recognition mechanism was probed using capillary electrophoresis, nuclear magnetic resonance (NMR), and molecular modeling techniques.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Chemistry, Analytical
Benjamin D. Slater, Matthew R. Hill, Bradley P. Ladewig
Summary: Solvent-induced enantioselectivity reversal is a rarely reported phenomenon in porous homochiral materials, but has been observed for the first time in a homochiral metal organic framework. This interesting selectivity reversal behavior may be observed in other competitive adsorption and nonchiral selective processes involving solvents.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Physics, Fluids & Plasmas
Dibyajyoti Mohanta, Debaprasad Giri, Sanjay Kumar
Summary: This study investigates the migration of a polymer chain across an entropic trap using the lattice model and exact enumeration technique. The effects of solvent gradient in the trap on the migration process are explored.
Review
Biochemical Research Methods
Paola Peluso, Bezhan Chankvetadze
Summary: Cyclodextrins (CDs) are versatile cyclic oligosaccharides obtained from starch degradation, with applications in various industries due to their hydrophilic and hydrophobic regions. Their inherent chirality allows for successful use as chiral selectors, while multidisciplinary approaches have shed light on recognition mechanisms. Computational techniques have become useful in modeling molecular dynamics, binding energies, and noncovalent interactions between CD and enantiomers.
Article
Biochemistry & Molecular Biology
N. J. Gumede, W. Nxumalo, K. Bisetty, L. Escuder Gilabert, M. J. Medina-Hernandez, S. Sagrado
BIOORGANIC CHEMISTRY
(2020)
Article
Biochemical Research Methods
Mireia Perez-Baeza, Laura Escuder-Gilabert, Yolanda Martin-Biosca, Salvador Sagrado, Maria Jose Medina-Hernandez
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Biochemical Research Methods
Mireia Perez-Baeza, Laura Escuder-Gilabert, Yolanda Martin-Biosca, Salvador Sagrado, Maria Jose Medina-Hernandez
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Chemistry, Analytical
Yolanda Martin-Biosca, Laura Escuder-Gilabert, Salvador Sagrado, Maria Jose Medina-Hernandez
Summary: The study aimed to investigate the enantioselectivity in the biodegradation of two common chiral drugs, citalopram and verapamil, using HS-gamma-CD as a chiral selector in Capillary Electrophoresis. The results suggest that citalopram could be potentially persistent while verapamil is presumed to be a non-persistent compound, and no evidence of enantioselectivity was observed in any of the biodegradation processes.
Article
Biochemical Research Methods
Mireia Perez-Baeza, Yolanda Martin-Biosca, Laura Escuder-Gilabert, Maria Jose Medina-Hernandez, Salvador Sagrado
Summary: Artificial neural networks are used to quantify the enantioresolution of chiral molecules in cellulose based on their structural information. The study develops a relatively simple ANN model with good predictive performance, and identifies the importance of surface tension and -NHR groups in explaining the enantioresolution under the current conditions.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Mireia Perez-Baeza, Laura Escuder-Gilabert, Yolanda Martin-Biosca, Salvador Sagrado, Maria Jose Medina-Hernandez
Summary: This study investigates the retention behavior and enantioresolution of 54 structurally unrelated neutral and basic compounds using cellulose-based chiral stationary phases and hydro-organic mobile phases. The results show that the choice of mobile phase and the nature of the compounds have a significant impact on the enantioresolution of the chromatographic systems.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Mireia Perez-Baeza, Laura Escuder-Gilabert, Yolanda Martin-Biosca, Salvador Sagrado, Maria Jose Medina-Hernandez
Summary: This paper explores the potential of micellar solutions of the anionic surfactant sodium dodecyl sulfate (SDS) as eluents in dispersive micro-solid phase extraction (D-mu SPE) and compares them with conventional hydro-organic eluents. The results demonstrate the suitability of Fe3O4 @PDA NPs as a sorbent for the extraction of antidepressants and the advantages of using SDS micellar solutions over classic hydro-organic eluents.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Yolanda Martin-Biosca, Laura Escuder-Gilabert, Mireia Perez-Baeza, Salvador Sagrado, Maria Jose Medina-Hernandez
ENVIRONMENTAL CHEMISTRY
(2020)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)