Article
Chemistry, Inorganic & Nuclear
Huimin Zhong, Cong Jiang, Jiahui Zou, Guancheng Zhu, Yanyan Huang
Summary: In this study, CeO2-based porous carbonaceous frameworks (CeO2 PCFs) were used as antioxidant nanozymes to scavenge reactive oxygen species and absorb benzo[a]pyrene. CeO2 PCFs exhibited excellent superoxide dismutase-like and catalase-like activity, effectively eliminating superoxide radical, hydroxyl radical, and hydrogen peroxide. The 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) free radical scavenging assay confirmed the free radical scavenging ability of CeO2 PCFs. Furthermore, CeO2 PCFs with large surface area and porous structure efficiently adsorbed benzo[a]pyrene. The designed CeO2 PCFs can serve as new antioxidant nanozymes for scavenging reactive oxygen species and adsorbing polycyclic aromatic hydrocarbons (PAHs).
INORGANIC CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Bozena Bukowska, Katarzyna Mokra, Jaromir Michalowicz
Summary: Benzo[a]pyrene (B[a]P) is a representative polycyclic aromatic hydrocarbon (PAH) that is commonly found in the environment and human surroundings. It has been shown to have toxic effects on DNA, reproduction, neurology, and development. The metabolism of B[a]P is mediated by cytochrome P450 (CYP P450) and its polymorphism determines the sensitivity to B[a]P. Additionally, B[a]P may facilitate the replication of viruses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Environmental Sciences
Javier Zurita, Hitesh V. Motwani, Leopold L. Ilag, Vassilis L. Souliotis, Soterios A. Kyrtopoulos, Ulrika Nilsson, Margareta Tornqvist
Summary: Electrophilic diol epoxide metabolites play a role in the carcinogenicity of benzo[a]pyrene. We propose an HPLC-MS method to measure stable blood protein adducts of benzo[a]pyrene diol epoxide at low levels, which is difficult to achieve using current analytical methods. This method has the potential to improve risk assessment related to exposure to benzo[a]pyrene.
Article
Environmental Sciences
Amor Hedfi, Manel Ben Ali, Montaser M. Hassan, Bander Albogami, Samia S. Al-Zahrani, Ezzeddine Mahmoudi, Paraskevi K. Karachle, Melissa Rohal-Lupher, Fehmi Boufahja
Summary: The majority of experimental studies on the effects of pollutants on meiofauna have been conducted in closed systems, with the current study revealing that single PAHs impact meiobenthic nematodes similarly in closed or open systems, with higher toxicity observed for benzo[a]pyrene. Closed systems contaminated with PAHs became organically enriched, resulting in changes in the types and numbers of deposit feeders. The responses of taxa to hydrocarbons in open systems were influenced by caudal morphology, with the effects of PAHs mixtures being both additive and synergistic. Based on this study, open systems are preferred over closed systems for more accurate research outcomes.
ENVIRONMENTAL POLLUTION
(2021)
Article
Chemistry, Multidisciplinary
Jingnan Jin, Yahui Shi, Baozhong Zhang, Dongjin Wan, Qingye Zhang, Ying Li
Summary: In this study, a BaP-degrading strain Pannonibacter sp. JPA3 was isolated from oil well production water, which could remove 80% of BaP at an initial concentration of 100 mg L-1 after 35 days of culture. The metabolites BaP-4,5-dihydrodiol, BaP-4,5-epoxide, 5-hydroxychrysene, and 2-hydroxy-1-naphthoic acid were identified in the biodegradation process. A dioxygenase model was built and molecular docking was performed to investigate the interaction mechanism between BaP and dioxygenase. A hypothesis for the progressive degradation mechanism of BaP by this strain was proposed based on the identification of downstream metabolites. In conclusion, this study provided an efficient BaP degrader and a comprehensive reference for the study of degradation mechanisms at the molecular level.
Article
Environmental Sciences
Barbara Campos Jorge, Ana Carolina Casali Reis, Erika Tissiana Sterde, Paola da Silva Balin, Wellerson Rodrigo Scarano, Hamilton Hisano, Arielle Cristina Arena
Summary: BaP exposure during the prepubertal period negatively impacted the reproductive parameters in adult male rats, leading to compromised copulatory behavior, reduced sperm quality and quantity, and histopathological changes in testicular tissue. A comprehensive risk assessment of prepubertal BaP exposure is necessary to understand its long-term effects on male reproductive health.
Article
Environmental Sciences
Claire Baranger, Isabelle Pezron, Laurence Lins, Magali Deleu, Anne Le Goff, Antoine Fayeulle
Summary: Hydrophobic organic soil contaminants like PAH are poorly mobile in soil and filamentous fungi are efficient in degrading them, but the mechanism of their mobilization needs further investigation. A microfluidic device was designed to study the uptake of BaP by fungi, revealing the uptake and storage mechanisms as well as the release of surface-active compounds.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biotechnology & Applied Microbiology
Mercedes Quintanilla-Mena, Julio Vega-Arreguin, Marcela Del Rio-Garcia, Victoria Patino-Suarez, Santy Peraza-Echeverria, Carlos Puch-Hau
Summary: The study investigated the effects of BaP on the gut microbiome of Oreochromis niloticus. Dysbiosis was observed at 24 hours post-injection, followed by a slight recovery at 72 hours and stabilization at 120 hours. The gut microbiome played a significant role in PAH degradation mainly at 24 hours post-injection.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Environmental Sciences
Rosa Posada-Baquero, Kirk T. Semple, Miguel Ternero, Jose-Julio Ortega-Calvo
Summary: The study utilized desorption extraction method standardized by ISO16751 to determine the bioavailable concentration of the commonly regulated polycyclic aromatic hydrocarbon benzo(a)pyrene (BaP) in industrial soil, showing comparable results to field-contaminated soils.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Chemistry, Multidisciplinary
Xiushang Xu, Amy L. Vonder Haar, Rengo Yoshioka, Qizheng Zhang, Serhii Vasylevskyi, Andrew J. Musser, Akimitsu Narita
Summary: A benzo[rst]pentaphene derivative, BPP-MeOPA, was synthesized and characterized by NMR and single-crystal X-ray analysis. Investigation of its photophysical properties, including transient absorption spectroscopy, revealed improved absorption and emission features from an S-1 state with excitonic and charge-transfer character by introducing MeOPA groups.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Physical
Yue Liu, Xinguo Ren, Lixin He
Summary: This study identified six possible DNA adducts with BPQ, including four covalently bound adducts and two adducts purely bound by van der Waals interactions. Surprisingly, the two van der Waals-bound adducts have comparable, if not larger, binding energies than the covalent adducts. These findings provide insights into the interactions between PAH metabolites and DNA.
JOURNAL OF CHEMICAL PHYSICS
(2021)
Article
Environmental Sciences
Dmitry E. Lakhmanov, Aleksandr Yu. Kozhevnikov, Sergey A. Pokryshkin, Igor P. Semiletov, Dmitry S. Kosyakov
Summary: This study represents the first investigation in 25 years of the lesser-known Siberian arctic seas, revealing the highest levels of PAHs in the Laptev Sea and a generally safe environmental situation in the region.
MARINE POLLUTION BULLETIN
(2022)
Article
Environmental Sciences
Dmitry E. Lakhmanov, Aleksandr Yu Kozhevnikov, Sergey A. Pokryshkin, Igor P. Semiletov, Dmitry S. Kosyakov
Summary: This study conducted the first research in 25 years on the least studied and hard-to-reach areas of Siberian Arctic seas. It found that the concentration of polycyclic aromatic hydrocarbons (PAHs) is comparable to the data from the 1990s, indicating a long-term persistence of pollution despite a decline in global PAH emissions. The main sources of PAHs are coal/biomass and liquid fuel combustion.
MARINE POLLUTION BULLETIN
(2022)
Article
Environmental Sciences
Siwar Abouda, Omayma Missawi, Tiziana Cappello, Iteb Boughattas, Giuseppe De Marco, Maria Maisano, Mohamed Banni
Summary: Marine ecosystems are currently facing severe threats from multiple stressors, including microplastics and polycyclic aromatic hydrocarbons. This study assessed the toxicity of environmental relevant concentrations of microplastics and benzo[a]pyrene, and found that they can accumulate in marine organisms and cause cytotoxicity, genotoxicity, and oxidative stress effects.
ENVIRONMENTAL POLLUTION
(2022)
Review
Environmental Sciences
Jiezhang Mo, Doris Wai-Ting Au, Jiahua Guo, Christoph Winkler, Richard Yuen-Chong Kong, Frauke Seemann
Summary: Research indicates that environmental exposure to benzo[a]pyrene may cause osteotoxicity, which could be transgenerationally inherited. Genetic and epigenetic factors play important roles in regulating bone metabolism, and researchers propose two possible epigenetic regulatory circuits in relation to bone miRNAs and genes.
CRITICAL REVIEWS IN ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2022)
Editorial Material
Chemistry, Multidisciplinary
Chloe Morel, Henri Schroeder, Claude Emond, Jonathan D. Turner, Eric Lichtfouse, Nathalie Grova
ENVIRONMENTAL CHEMISTRY LETTERS
(2023)
Article
Endocrinology & Metabolism
Feng-Jiao Peng, Paul Palazzi, Sakina Mezzache, Nasrine Bourokba, Jeremie Soeur, Brice M. R. Appenzeller
Summary: In this study, a method for analyzing multiple endogenous hormones in human hair was developed and validated. The method allowed the detection of 32 hormones from different chemical classes and established complete reference intervals. Correlations between certain hormone concentrations in hair were found. This method is of significance for better identifying endocrine disorders.
EUROPEAN JOURNAL OF ENDOCRINOLOGY
(2022)
Article
Environmental Sciences
Sabrina Krief, Alba Iglesias-Gonzalez, Brice M. R. Appenzeller, Lyna Rachid, Marielle Beltrame, Edward Asalu, John-Paul Okimat, Nicole Kane-Maguire, Petra Spirhanzlova
Summary: The study found that both wild and captive chimpanzees are exposed to a variety of organic pollutants through their diet. Captive chimpanzees had significantly higher concentrations of detected chemicals compared to wild chimpanzees. However, reducing exposure to pollutants in their environment resulted in a decrease in the number and concentrations of chemicals detected in captive chimpanzees.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Environmental Sciences
Liese Gilles, Eva Govarts, Laura Rodriguez Martin, Anna-Maria Andersson, Brice M. R. Appenzeller, Fabio Barbone, Argelia Castano, Dries Coertjens, Elly Den Hond, Vazha Dzhedzheia, Ivan ErZen, Marta Esteban Lopez, Lucia Fabelova, Clemence Fillol, Carmen Franken, Hanne Frederiksen, Catherine Gabriel, Line Smastuen Haug, Milena Horvat, Thorhallur Ingi Halldorsson, Beata Janasik, NataSa Janev Holcer, Reka Kakucs, Spyros Karakitsios, Andromachi Katsonouri, Jana Klanova, Tina Kold-Jensen, Marike Kolossa-Gehring, Corina Konstantinou, Jani Koponen, Sanna Lignell, Anna Karin Lindroos, Konstantinos C. Makris, Darja Mazej, Bert Morrens, Lubica PalkoviCova Murinova, Sonia Namorado, Susana Pedraza-Diaz, Jasmin Peisker, Nicole Probst-Hensch, Loic Rambaud, Valentina Rosolen, Enrico Rucic, Maria Ruether, Dimosthenis Sarigiannis, Janja Snoj Tratnik, Arnout Standaert, Lorraine Stewart, Tamas Szigeti, Cathrine Thomsen, Hanna Tolonen, Asa Eiriksdottir, An Van Nieuwenhuyse, Veerle J. Verheyen, Jelle Vlaanderen, Nina Vogel, Wojciech Wasowicz, Till Weber, Jan-Paul Zock, Ovnair Sepai, Greet Schoeters
Summary: This study describes the organization of the first HBM4EU-aligned studies in Europe, aiming to monitor the internal exposure of European citizens to environmental chemicals by collecting comparable human biomonitoring data.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Environmental Sciences
Alba Iglesias-Gonzalez, Mylene Schweitzer, Paul Palazzi, Fengjiao Peng, Serge Haan, Elisabeth Letellier, Brice M. R. Appenzeller
Summary: The specific physiology and behavior of children make them more vulnerable to chemical exposure, hence the need for specific studies to understand the impact of pollution on children's health. This study used human biomonitoring with hair samples to assess chemical exposure and investigate possible determinants in children.
ENVIRONMENT INTERNATIONAL
(2022)
Article
Environmental Sciences
Michael Poteser, Federica Laguzzi, Thomas Schettgen, Nina Vogel, Till Weber, Aline Murawski, Phillipp Schmidt, Maria Ruther, Marike Kolossa-Gehring, Sonia Namorado, An Van Nieuwenhuyse, Brice Appenzeller, Edda Dufthaksdottir, Kristin Olafsdottir, Line Smastuen Haug, Cathrine Thomsen, Fabio Barbone, Valentina Rosolen, Loic Rambaud, Margaux Riou, Thomas Goen, Stefanie Nuebler, Moritz Schaefer, Karin H. A. Zarrabi, Liese Gilles, Laura Rodriguez Martin, Greet Schoeters, Ovnair Sepai, Eva Govarts, Hanns Moshammer
Summary: This report summarizes the results of the HBM4EU project and associated studies regarding acrylamide exposure levels. The findings show an increasing trend in acrylamide exposure in children, while the trend is less significant in adults and smokers. The average acrylamide exposure still exceeds suggested benchmark levels, raising specific concerns for children. Further research is needed to confirm the declining trend observed in recent years.
Article
Environmental Sciences
Feng-Jiao Peng, Paul Palazzi, Catherine Viguie, Brice M. R. Appenzeller
Summary: This study used hair analysis to assess the impact of a pesticide mixture on hormone levels in female rats and found that low-level exposure can alter reproductive and adrenal hormone levels in hair.
Article
Environmental Sciences
Feng-Jiao Peng, Paul Palazzi, Catherine Viguie, Brice M. R. Appenzeller
Summary: This study used hair analysis to assess the combined effects of 13 PAHs on steroid and thyroid hormones levels in a rat model. The results showed that PAH mixtures can interfere with steroid and thyroid hormone levels in female rats, and their effects are related to exposure intensity and hydroxylated PAHs concentrations.
ENVIRONMENTAL POLLUTION
(2022)
Article
Biochemical Research Methods
Claire Roseren, Sylvia Binck, Francois Fays, Maria Ruiz-Castell, Hanen Samouda, Brice M. R. Appenzeller
Summary: Despite efforts to reduce tobacco consumption, smoking remains a leading cause of death for both smokers and nonsmokers exposed to environmental tobacco smoke (ETS). This study investigates the determinants of ETS in young adult students, who are considered at higher risk of exposure due to their specific lifestyle. The results highlight the widespread exposure to ETS in this population and emphasize the importance of exposure assessment and hair analysis.
DRUG TESTING AND ANALYSIS
(2023)
Letter
Biochemical Research Methods
Donata Favretto, Gail Cooper, Maristela Andraus, Frank Sporkert, Ronald Agius, Brice Appenzeller, Markus Baumgartner, Tina Binz, Vincent Cirimele, Robert Kronstrand, Maria del Mar Ramirez, Sabina Strano-Rossi, Michael Uhl, Marco Vincenti, Michel Yegles
DRUG TESTING AND ANALYSIS
(2023)
Article
Engineering, Environmental
Francois Fays, Paul Palazzi, Florence Zeman, Emilie M. Hardy, Charline Schaeffer, Christophe Rousselle, Claire Beausoleil, Brice M. R. Appenzeller
Summary: This study assesses the relevance of hair analysis for biomonitoring exposure to fast-elimination compounds and investigates the role of pharmacokinetics in their incorporation in hair.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Medicinal
Baninia Habchi, Sandra Alves, Sylvie Streel, Michele Guillaume, Anne-Francoise Donneau, Brice M. R. Appenzeller, Douglas N. Rutledge, Alain Paris, Estelle Rathahao-Paris
Summary: This study used a high-throughput approach to analyze the metabolomic data of samples collected in epidemiological studies. It identified exposure markers and confirmed their correlation with drug concentrations. The results demonstrate the potential of this method for rapid phenotyping in large-scale epidemiological studies.
CHEMICAL RESEARCH IN TOXICOLOGY
(2023)
Editorial Material
Biochemical Research Methods
Donata Favretto, Brice M. R. Appenzeller, Vincent Cirimele, Sabina Strano Rossi
DRUG TESTING AND ANALYSIS
(2023)
Article
Engineering, Environmental
Feng-Jiao Peng, Paul Palazzi, Sakina Mezzache, Emilie Adelin, Nasrine Bourokba, Philippe Bastien, Brice M. R. Appenzeller
Summary: Studies have shown that exposure to organic pollutants may alter sex steroid hormone levels in animals and humans. However, research on the mixture effects of these pollutants is limited, and the focus has been mainly on persistent organic pollutants. In this study, hair analysis was used to evaluate the relationship between multiclass organic pollutants and sex steroid hormones in healthy Chinese women. The results showed that each hormone was associated with a mixture of at least 10 examined pollutants.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Clementine Fritsch, Brice Appenzeller, Louisiane Burkart, Michael Coeurdassier, Renaud Scheifler, Francis Raoul, Vincent Driget, Thibaut Powolny, Candice Gagnaison, Dominique Rieffel, Eve Afonso, Anne-Claude Goydadin, Emilie M. Hardy, Paul Palazzi, Charline Schaeffer, Sabrina Gaba, Vincent Bretagnolle, Colette Bertrand, Celine Pelosi
Summary: This study investigated the exposure of free-ranging mammals to pesticides and found widespread contamination, which has implications for ecosystem impacts. The results suggest that wildlife exposure to pesticide mixtures is a common occurrence, highlighting the need to consider the concept of environmental exposure and question the adequacy of current risk assessment and mitigation processes.
SCIENTIFIC REPORTS
(2022)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)