Article
Chemistry, Applied
Hui Wang, Lili Hu, Xiaotu Chang, Yuge Hu, Yan Zhang, Peng Zhou, Xiaojiao Cui
Summary: A method using UHPLC-MS/MS was developed for the determination of toxoflavin and fervenulin in 6 types of food, and the degradation products were identified.
Article
Biochemistry & Molecular Biology
Michal Wronski, Jakub Trawinski, Lukasz Komsta, Robert Skibinski
Summary: Chemometric analysis identified nine forced degradation products of maraviroc. This study subjected the antiretroviral drug to various stress conditions such as photolysis, oxidation, hydrolysis, and electrochemical transformation. It was found that maraviroc is susceptible to UVA, H2O2, and electrochemical degradation, while being resistant to hydrolysis. Electrochemical transformation, especially on the platinum electrode, partially mimics the forced degradation processes, particularly in terms of redox reactions. These findings suggest that electrochemical methods can be cost-effective and rapid supplements to commonly used forced degradation procedures.
Article
Biochemistry & Molecular Biology
Wei Wei, Siwei Li, Erwei Hao, Xianglong Pan, Jinling Xie, Zhengcai Du, Xiaotao Hou, Jiagang Deng
Summary: In this study, an integrated research strategy combining UHPLC-Q-TOF-MSE method and data post-processing strategy was used to rapidly analyze Compound Huanggen Granules (CHG) and absorbed prototypes in plasma. By applying this strategy, multiple compounds were identified and their structures were characterized. These results could provide valuable insights for further studies on the quality control, pharmacokinetics and pharmacodynamics of CHG.
CURRENT DRUG METABOLISM
(2022)
Article
Chemistry, Applied
Sha Yan, Xuan Wang, Yuchen Wu, Kai Wang, Jihao Shan, Xiaofeng Xue
Summary: By analyzing the metabolomics of acacia honey, a compound called Fru-Phe was found to be a potential indicator for distinguishing artificially heated acacia honey from naturally matured acacia honey.
Article
Chemistry, Analytical
Wenjing Liu, Mengqin Xia, Jing Bai, Li Yang, Zhengtao Wang, Rui Wang, Yanhong Shi
Summary: The study identified common and predominant phenolic components in different varieties of Lycium fruits and characterized the phenolics profiles for each Lycium species, which can be utilized for fruit identification. Furthermore, the inhibitory activity against 5 alpha-reductase showed that the Lycium barbarum L. extract was relatively more effective.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Food Science & Technology
Guiren Xue, Shanshan Su, Pengfei Yan, Jiawei Shang, Jianxin Wang, Chengye Yan, Jiaxi Li, Qiao Wang, Yingfeng Du, Liang Cao, Huijun Xu
Summary: This study investigated the chemical composition differences of Zingiberis Rhizoma (ZR), Zingiberis Rhizoma Praeparatum (ZRP), and Carbonised Ginger (CG) through non-targeted metabolomics analysis. A total of 59 gingerols were identified, and 33 of them were nominated as novel markers for materials authentication. The findings showed that the content of gingerols decreased after processing, while shogaols and gingerone compounds increased, resulting in differences in application and pharmacodynamic efficacy.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Chemistry, Applied
Jinglei Wang, Qingzhen Wei, Wuhong Wang, Haijiao Hu, Yaqin Yan, Yikui Wang, Yan Li, Yaqin Jiang, Guanzheng Wu, Tianhua Hu, Chonglai Bao
Summary: This study identified metabolites in radishes of ten varieties using UHPLC-Q-TOF-MS, and found that the metabolite composition differed among different edible types, with greater accumulation in fruit radishes, particularly flavonoids.
Article
Chemistry, Analytical
Mei Wang, Jianping Zhao, Bharathi Avula, Joseph Lee, Roy Upton, Ikhlas A. Khan
Summary: This study investigated the chemical characteristics of Eriodictyon species, commonly known as yerba santa, and found that they contain a rich variety of chemical compounds, particularly flavonoids and phenolic acids, which have medicinal value. The study provides a foundation for chemical characterization and quality assessment of this plant species.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Hongxue Gao, Zhiqiang Liu, Fengrui Song, Junpeng Xing, Zhong Zheng, Shu Liu
Summary: This study established an integrated strategy for the separation and identification of chemical components in Plantago asiatica L. The strategy involved three-dimensional separation using AB-8 macroporous resin column chromatography, UHPLC-Q-TOF MS, and UHPLC-IM-MS. By utilizing this strategy, a total of 86 compounds, including various classes of compounds, were successfully identified from PAL. Additionally, the strategy allowed for the detection and identification of isomers. This proposed method provides an effective approach for the separation and identification of chemical components in traditional Chinese medicine.
Article
Immunology
Nan Wang, Linjiao Yang, Lili Shang, Zhaojun Liang, Yanlin Wang, Min Feng, Shuting Yu, Xiaoying Li, Chong Gao, Zhenyu Li, Jing Luo
Summary: This study identified potential diagnostic biomarkers for Psoriatic Arthritis (PsA) by analyzing fecal metabolites in comparison with Rheumatoid Arthritis (RA) and healthy controls. A total of 14 common differential metabolites were defined as candidate biomarkers, with 5 fecal metabolites determined as specific biomarkers for PsA through a support vector machines (SVM) model. Abnormality in amino acid metabolism, bile acid metabolism and lipid metabolism were found to contribute to the occurrence and development of PsA.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemical Research Methods
Jiading Guo, Lei Zhang, Ye Shang, Xiaohua Yang, Jin Li, Jun He, Xiumei Gao, Yan-xu Chang
Summary: With a focus on the phytochemical constituents and quality evaluation of QiangHuoShengShi (QHSS) decoction, this study developed an integrated strategy combining mass spectrum analysis, compound identification, and target analyte quantification for precise characterization of the TCM formulae.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Min Li, Feng-Xiang Zhang, Zhuo-Chun Wei, Zi-Ting Li, Guo-Xun Zhang, Hai-Jun Li
Summary: This study characterized the metabolism features of Ziziphi Spinosae Semen (ZSS) in rats using UHPLCR-TOF MS method, identifying a total of 151 xenobiotics (58 prototypes and 93 metabolites) in rats after ingestion of ZSS. Major metabolic reactions of ZSS in rats were hydrogenation, hydrolysis, and glucuronidation. The study provided valuable data for further research on the pharmacological mechanism and pharmacokinetics evaluation of ZSS.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Chemistry, Applied
Bei Zhou, Wen Huang, Xi Feng, Qian Liu, Salam A. Ibrahim, Ying Liu
Summary: This study investigated glucosinolate variations in Chinese cabbage cultivars at different growth periods. Thirteen glucosinolates were identified and quantified, and significant differences in glucosinolate concentrations were found between Xiayangbai and Zaoshu-5. The highest concentration of glucosinolates was observed during the seed period, with aliphatic glucosinolates being predominant in seeds, seedling, and leaves of the rosette as well as during germination. The roots, however, contained a dominant aromatic glucosinolate (gluconasturtiin), and glucoerucin was only found in the roots of rosettes. Positive significant correlations were observed among gluconapin, glucobrassicanapin, glucoraphanin, glucoalyssin, and 4-hydroxyglucobrassicin. These findings provide scientific evidence for the metabolism pathways of glucosinolates in Chinese cabbage and can contribute to the development of functional foods with higher glucosinolate content.
Article
Chemistry, Analytical
Tinglan Zhang, Jun Zhang, Fangfang Chen, An Liu, Jinzhu Jiang, Zhiyong Yan, Xianju Liu
Summary: In this study, we analyzed the triterpenoid compounds in different fresh tissues of Pulsatilla chinensis using UHPLC-Q-TOF-MS and UHPLC-QQQ-MS techniques. A total of 132 triterpenoids were identified, including new aglycones and new ways of rhamnose linking. We established an analytical method for triterpenoid content and found significant distribution differences in types and contents of triterpenoids among different tissues. We also identified 15 differential components between aboveground and underground tissues. This study provides an efficient analysis strategy for triterpenoids in P. chinensis and important insights into the biosynthetic pathway of triterpenoid saponins.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Analytical
Gemma Rosello-Marquez, Ramon Manuel Fernandez-Domene, Jose Garcia-Anton
Summary: The photoelectrocatalytic degradation of three organophosphorus pesticides using innovative WO3 nanostructure as photoanode showed high efficiency and led to proposed degradation pathways for each pesticide based on identified compounds.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Maria Ramirez-Ambrosi, Diana M. Lopez-Marquez, Beatriz Abad-Garcia, Enrique Dapena, Luis A. Berrueta, Blanca Gallo
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2015)
Article
Food Science & Technology
Z. Rasines-Perea, N. Prieto-Perea, M. Romera-Fernandez, L. A. Berrueta, B. Gallo
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2015)
Article
Biochemical Research Methods
Gabriela E. Viacava, Sara I. Roura, Luis A. Berrueta, Carmen Iriondo, Blanca Gallo, Rosa M. Alonso-Salces
JOURNAL OF MASS SPECTROMETRY
(2017)
Article
Biochemistry & Molecular Biology
Cindy Quaglieri, Noelia Prieto-Perea, Luis Angel Berrueta, Blanca Gallo, Zurine Rasines-Perea, Michael Jourdes, Pierre-Louis Teissedre
Article
Chemistry, Analytical
Luis A. Berrueta, Andrea Sasia-Arriba, Marcos Minarro, Maria J. Anton, Rosa M. Alonso-Salces, Diego Micheletti, Blanca Gallo, Enrique Dapena
Article
Biochemical Research Methods
Thiago Wolff, Luis A. Berrueta, Ligia M. M. Valente, Rodolfo S. Barboza, Romulo L. S. Neris, Iris P. Guimaraes-Andrade, Iranaia Assuncao-Miranda, Adriana C. Nascimento, Mario Gomes, Blanca Gallo, Carmen Iriondo
PHYTOCHEMICAL ANALYSIS
(2019)
Letter
Dermatology
Jone Garate, Sergio Lage, Roberto Fernandez, Veronica Velasco, Beatriz Abad, Aintzane Asumendi, Jesus Gardeazabal, Yoana Arroyo-Berdugo, Maria Angeles Rodriguez, Juan Luis Artola, Ignacio Zabalza, Begona Ochoa, Jose A. Fernandez, Maria Dolores Boyano
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2019)
Article
Food Science & Technology
Rosa Maria Alonso-Salces, Blanca Gallo, Maria Isabel Collado, Andrea Sasia-Arriba, Gabriela Elena Viacava, Diego Luis Garcia-Gonzalez, Tullia Gallina Toschi, Maurizio Servili, Luis Angel Berrueta
Summary: Metabolomic fingerprinting of virgin olive oil by H-1 NMR spectroscopy revealed that light exposure and increasing temperature enhance the oxidative degradation process in VOO. Analysis of chemical compounds and signal changes provided insights into VOO freshness evaluation and storage time determination.
Article
Biochemistry & Molecular Biology
Arantza Perez-Valle, Beatriz Abad-Garcia, Olatz Fresnedo, Gabriel Barreda-Gomez, Patricia Aspichueta, Aintzane Asumendi, Egoitz Astigarraga, Jose A. Fernandez, Maria Dolores Boyano, Begona Ochoa
Summary: By studying the lipidomes of normal human epidermal and nevus melanocytes, as well as primary and metastatic melanoma cell lines, significant and highly specific changes in over 30 lipid species were identified in the initiation of melanoma. Notably, malignancy lipid signatures showed marked drops in pivotal membrane lipids like sphingomyelins, suggesting a previously undefined remodeling of sphingolipid and glycerophospholipid metabolism. These different lipid profiles may aid in clinical diagnosis/prognosis and therapeutic interventions for cutaneous melanoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Applied
Ariel M. M. Araujo, Lucas M. M. Cruz, Mario Gomes, Blanca Gallo, Ligia M. M. Valente, Luis A. A. Berrueta
Summary: This study reports for the first time the phytochemical and biological evaluation of Bathysa gymnocarpa, an endemic tree species in Brazil. Through HPLC-DAD-ESI-MS/MS analysis, fourteen compounds were identified, including two cinnamic acid derivatives and several glycosylated derivatives of the flavonols quercetin and kaempferol. These compounds are reported for the first time in Bathysa spp.
NATURAL PRODUCT RESEARCH
(2023)
Article
Plant Sciences
Sarah Neves do Nascimento, Jose Luiz Mazzei, Joao Batista de Freitas Tostes, Batista de Freitas Tostes, Marcos Jun Nakamura, Ligia Maria Marino Valente, Raquel Curtinhas de Lima, Priscila Conrado Guerra Nunes, Elzinandes Leal de Azeredo, Luis Angel Berrueta, Blanca Gallo, Antonio Carlos Siani
Summary: Miconia albicans is widely used in Brazil as a remedy for rheumatoid arthritis and to alleviate symptoms caused by Chikungunya virus. This study found that certain components of this plant have inhibitory effects on CHIKV.
JOURNAL OF ETHNOPHARMACOLOGY
(2024)
Article
Chemistry, Multidisciplinary
Djavan da Paixao, Rodolfo S. Barboza, Ligia M. M. Valente, Matheus O. Souza, Antonio C. Siani, Rita C. A. Pereira, Blanca Gallo, Luis A. Berrueta
Summary: This study quantitatively evaluated the kaempferitrin content in leaves and branches of cultivated and wild UG specimens from the Brazilian Amazon rainforest, revealing its high concentration in leaves and its potential as a chemical marker for UG products. The analysis also identified quercetin-3,7-O-(alpha)-L-dirhamnoside in UG for the first time, highlighting the importance of kaempferitrin as a marker for this species.
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Multidisciplinary
Jessica O. Costa, Rodolfo S. Barboza, Ligia M. M. Valente, Thiago Wolff, Mario Gomes, Blanca Gallo, Luis A. Berrueta, Iris P. Guimaraes-Andrade, Daniel Gavino-Leopoldino, Iranaia Assuncao-Miranda
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
(2020)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)