Article
Biochemistry & Molecular Biology
Nikolay Petkov, Ivayla Pantcheva, Anela Ivanova, Radostina Stoyanova, Rositsa Kukeva, Radostina Alexandrova, Abedullkader Abudalleh, Petar Dorkov
Summary: Novel coordination species of monensic and salinomycinic acids with Ce4+ ions were synthesized and characterized. The Ce(IV) complexes showed selective cytotoxic activity against HeLa cell line.
Article
Veterinary Sciences
Marcelo Baggio, Vinicius N. Gouvea, Jose Paulo R. Barroso, Alexandre A. Miszura, Arnaldo C. Limede, Leticia C. B. Soares, Marcos Vinicius C. Ferraz, Ana Carolina S. Vicente, Evandro M. Ferreira, Rodrigo S. Marques, Alexandre V. Pires
Summary: The objective of this study was to evaluate the effects of different combinations of monensin and narasin on finishing cattle. The study found that the narasin group had higher feed intake during the feeding period compared to the monensin group, but these additives did not affect the digestibility of nutrients, growth performance, or carcass characteristics of the cattle.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Infectious Diseases
Lisa Knoche, Jan Lisec, Tanja Schwerdtle, Matthias Koch
Summary: This study systematically investigated the formation and identification of transformation products (TPs) of the drug salinomycin (SAL) using electrochemistry and liver microsome incubation methods. A total of 14 new TPs were found, including decarbonylation for electrochemistry-derived TPs and oxidation for liver microsome-derived TPs. The identification of potassium-based TPs is of particular interest as they may have been overlooked in previous studies. The obtained knowledge contributes to understanding the transformation reactions and predicting the structure of SAL metabolites.
Article
Virology
Yoshikazu Tanaka, Eri Tanabe, Yuki Nonaka, Mitsuki Uemura, Tsuyoshi Tajima, Kazuhiko Ochiai
Summary: Feline coronaviruses (FCoVs) are a global infection in cats, causing severe systemic diseases. The current drugs for treating FIP are ineffective, highlighting the need to investigate host factors and replication functions for FCoV in order to develop effective drugs. Ionophore antibiotics, by altering the pH conditions in the endosomes, inhibit FCoV proliferation in vitro, showing potential as broad-spectrum anti-FCoV agents.
Article
Chemistry, Medicinal
Dominika Czerwonka, Yzobelle Barcelos, Dietmar Steverding, Aleksandra Cioch, Adam Huczynski, Michal Antoszczak
Summary: Polyether ionophores, such as salinomycin, exhibit a broad range of pharmacological properties with promising activity against various parasites. Studies on the anti-parasitic activity of salinomycin derivatives are limited, but recent findings suggest that modifications at the C20 position can enhance trypanocidal activity.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Food Science & Technology
Victor A. A. Pastore, Flavio A. Santos, Mary Ane G. Lana, Guilherme R. Silva, Tadeu C. Figueiredo, Debora C. S. Assis, Silvana Cancado
Summary: A validated multiresidue method was developed to monitor the residues of macrocyclic lactones, monensin, and fipronil in bovine and chicken livers, with good performance in terms of linearity, selectivity, and accuracy.
FOOD ANALYTICAL METHODS
(2022)
Article
Chemistry, Analytical
Yu-Zhu Chen, Bing-Su Niu, Bin Ji, Fang Fang, Xiao-Lin Guo, Zhi-Yong Wu
Summary: Charged species from complex media can be separated and enriched using the ion concentration polarization (ICP) effect, making it possible to directly process salty physiological samples such as blood and urine. A novel and reliable ICP interface was successfully established on a paper-based analytical device (PAD) for the electrokinetic stacking of target analytes, with promising results for online stacking of total protein from urine samples and image-based colorimetric detection by a smartphone camera.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Christopher M. Crittenden, Michael B. Lanzillotti, Bifan Chen
Summary: CRISPR-Cas9 genome editing has become a vital technique in biomedical research and shows significant therapeutic potential. Researchers have developed a simple and effective online column-based clean-up method to remove salts and metal adducts from larger oligonucleotide samples for mass spectrometry analysis. This method successfully sequenced 100-nucleotide-long sgRNAs, providing an easy approach for analyzing and sequencing chemically modified oligonucleotides.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Anna Siemiatkowska, Andrew Wassef, Ragui Sadek, Celine Park, Christine Yohn, Luigi Brunetti, Leonid Kagan
Summary: A novel and validated method was developed to determine cefazolin concentrations in human adipose tissue. Rat subcutaneous adipose tissue was found to be a suitable surrogate matrix for human adipose tissue. This method will be used in a large-scale study of non-obese and obese patients receiving prophylactic cefazolin before surgeries.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Veterinary Sciences
Antonio W. O. Silva, Mucio F. F. Mendonca, Moises D. Freitas, Antonio L. Ribeiro Filho, Ricardo D. G. Silva, Paula Leal, Luciano A. Pimentel, Tiago C. Peixoto
Summary: This study describes clinical-epidemiological and anatomopathological data of an outbreak of accidental poisoning by monensin in buffaloes. The outbreak resulted in severe symptoms, including muscle weakness, tremors, and death. Laboratory tests and histopathological examination confirmed the poisoning.
PESQUISA VETERINARIA BRASILEIRA
(2022)
Article
Veterinary Sciences
Amanda Q. Carvalho, Claudia S. Wisser, Guilherme O. Manfioletti, Natalia Rigo, Jose Cristani, Sandra D. Traverso
Summary: This study describes the spontaneous and experimental salinomycin poisoning associated with the use of florfenicol and warns about the effects of combining antibiotics and ionophores in animal feed. The results indicate that florfenicol can cause the accumulation of salinomycin in animals, leading to toxic myopathy.
PESQUISA VETERINARIA BRASILEIRA
(2022)
Article
Environmental Sciences
Yajie Tian, Jianzheng Li, Xianhui Li, Jiuling Li, Jia Meng
Summary: The developed method for simultaneous extraction and determination of eight veterinary antibiotics in swine wastewater and activated sludge showed good recoveries, low deviations, and high sensitivity. It has been successfully applied to evaluate the actual concentration levels of antibiotics in the two complex environmental matrices.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Chemistry, Applied
Shuhe Mu, Tengwang Teng, Ruidong Zhou, Huan Liu, Huiwu Sun, Jincheng Li
Summary: A novel semi-automatic pre-treatment apparatus was developed for rapid quantification of 18 antibiotics in fish samples. This apparatus allows for single-step clean-up of the samples, with up to 10 samples purified simultaneously. The purified analytes were then separated and quantified using HPLC-QTRAP-MS/MS.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Chemistry, Applied
Yuxiao Zhu, Fengshou Dong, Xingang Liu, Xiaohu Wu, Jun Xu, Yongquan Zheng
Summary: This study aimed to develop an effective multi-residue analysis method to reduce matrix effects in spices and herbs, and determine the main component causing matrix effects in black pepper. Among various methods to reduce matrix effects, dilution (5-fold) combined with d-SPE was more effective. Black pepper exhibited a stronger matrix effect compared to other spices.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2021)
Article
Materials Science, Multidisciplinary
A. Satya Prasad, S. Naveen Kumar, M. Akhila Maheswari, D. Prabhakaran
Summary: The synthesis of CeO2/La2O3 nanocomposites integrated with mesoporous TiO2 monoliths significantly reduces bandgap energy and promotes visible-light photocatalysis efficiency. The photocatalytic materials demonstrate excellent environmental remediation capabilities and reusability potential through thorough physico-chemical parameter studies and drug degradation analyses.
BULLETIN OF MATERIALS SCIENCE
(2021)
Article
Environmental Sciences
Xi Chen, Zhanjun Cheng, Guanyi Chen, Yongkui Yang, Peizhe Sun
Summary: Agricultural application is the main source of antibiotic consumption worldwide. Veterinary antibiotics, poorly metabolized by animals, end up in agricultural waste and are increasingly subjected to thermal treatment. However, there is a lack of research on the thermal decomposition mechanisms and products of these antibiotics.
ENVIRONMENTAL POLLUTION
(2023)
Article
Environmental Sciences
Yuanqi Liu, Zhuochao Liu, Dan Cui, Liming Yang, Haiyu Wang, Spyros G. Pavlostathis, Yanni Geng, Zhensheng Xiong, Penghui Shao, Xubiao Luo, Shenglian Luo
Summary: By adding a biodegradable carrier, loofah, the microalgae-bacteria symbiotic system achieved a 100% removal rate of high concentration ammonia nitrogen (NH4+-N) and showed good stability. The loofah also released a considerable amount of carbon that could support denitrification. The study expands our understanding of the enhanced role of carbon-based carriers in the denitrification of rare earth element tailings wastewater.
Article
Engineering, Environmental
Juhee Kim, Junyue Wang, Daniel C. Ashley, Virender K. Sharma, Ching-Hua Huang
Summary: This study discovers that the biodegradable chelating ligand picolinic acid (PICA) can effectively mediate the activation of peracetic acid (PAA) by manganese (II), leading to accelerated degradation of micropollutants. The PAA-Mn(II)-PICA system is capable of rapidly removing various micropollutants in clean and wastewater matrices. High-valent manganese species (Mn(V)) is identified as the main reactive species contributing to the rapid degradation of micropollutants.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Tongcai Liu, Shaoze Xiao, Nan Li, Jiabin Chen, Yao Xu, Wenjun Yin, Xuefei Zhou, Ching-Hua Huang, Yalei Zhang
Summary: New research shows that brominating agents (such as BrCl, Br-2, BrOCl, and Br2O) generated during the use of peracetic acid (PAA) for oxidation and disinfection play important roles in the transformation of micropollutants, even at lower concentrations than HOCl and HOBr. The presence of Cl- and Br- in the environment greatly accelerates the transformation of micropollutants by PAA. This study provides new knowledge about the specific reactivity of brominating agents and highlights their critical roles in micropollutant abatement and disinfection byproduct formation during PAA oxidation and disinfection.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Elliot Reid, Thomas Igou, Yangying Zhao, John Crittenden, Ching-Hua Huang, Paul Westerhoff, Bruce Rittmann, Jorg E. Drewes, Yongsheng Chen
Summary: Chlorine-based disinfection for drinking water treatment was a significant achievement in public health in the 20th century. However, it is now known that chlorinated drinking water is not completely safe due to the presence of disinfection byproducts and other contaminants. The Minus Approach offers alternative methods to remove these risks and improve the sustainability performance of water treatment.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Ching-Hua Huang, Junyue Wang, Wensi Chen, Ting Wang, Elliot Reid, Caroline Krall, Juhee Kim, Tianqi Zhang, Xing Xie
Summary: Peroxyacids (POAs) show potential as alternatives to chlorine for disinfection byproduct reduction, but further research is needed to understand their microbial inactivation capabilities and mechanisms of action. This study evaluated the efficacy of three POAs (performic acid, peracetic acid, and perpropionic acid) compared to chlor(am)ine for inactivating representative microorganisms and reacted with biomolecules. Results showed that POAs were more effective than chlorine for bacterial inactivation but less effective for viral inactivation. Mechanistic insights suggest that POAs interact selectively with certain biomolecules, which may explain their differential effectiveness against bacteria and viruses.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Chenxi Li, Yingxin Zhao, Yanxing Song, Xiaojie Qiu, Shuaize Wang, Peizhe Sun
Summary: In this study, an electron acceptor platform of Ag2O/La(OH)3/polyacrylonitrile (PAN) fibers was constructed using a heterojunction strategy and electrospinning technology to develop a novel photocatalytic membrane with a redesigned electron transport pathway. The optimized electron transport pathway included intercrystal electron transfer induced by the La-O bond between Ag2O and La(OH)3 as well as electron transfer between the catalyst crystal and electrophilic PAN membrane interface. The Ag2O/La(OH)3 membrane exhibited excellent photocatalytic performance and stability for tetracycline (TC) removal.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Physical
Virender K. Sharma, Junyue Wang, Mingbao Feng, Ching-Hua Huang
Summary: The occurrence of micropollutants in water poses a threat to public health and the environment. The addition of certain amino acids can enhance the removal efficiency of these micropollutants by ferrate(VI).
JOURNAL OF PHYSICAL CHEMISTRY A
(2023)
Article
Engineering, Environmental
Penghui Du, Wen Liu, Qiang Zhang, Peng Zhang, Chen He, Quan Shi, Ching-Hua Huang, Junjian Wang
Summary: This study systematically investigated the changes in characteristics and composition of dissolved organic matter (DOM) under UV/PAA treatment, as well as the underlying mechanisms. UV/PAA treatment significantly reduced the aromaticity, apparent molecular weight, and fluorescent abundance of DOM, while producing more oxidized and saturated compounds. The reactive species (.OH and CH3C(O)O./CH3C(O)OO.) play a primary role in DOM changes, but with differences in reaction selectivity and mechanisms.
Article
Engineering, Environmental
Jiale Xu, Ching-Hua Huang
Summary: Krypton chloride (KrCl*) excilamps emitting at far-UVC222 nm are a potential technology for microbial disinfection and oxidation of organic micropollutants (OMPs) in water treatment. In this study, photolysis of 46 OMPs by a KrCl* excilamp and a low-pressure mercury UV lamp was evaluated, and it was found that OMP photolysis was greatly enhanced at 222 nm compared to 254 nm. The photolysis rate constants and quantum yields at 222 nm were significantly higher for most OMPs. The enhanced photolysis at 222 nm was mainly attributed to strong light absorbance for non-nitrogenous, aniline-like, and triazine OMPs, while nitrogenous OMPs showed notably higher quantum yield. Humic acid can inhibit OMP photolysis at 222 nm by light screening and potentially quenching intermediates, while nitrate/nitrite may contribute more to light screening. Overall, KrCl* excilamps show promise for effective OMP photolysis and warrant further research.
ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS
(2023)
Article
Engineering, Environmental
Zhipeng Li, Xu Zhang, Yuan Meng, Hong Yao, Zhanjun Cheng, Guanyi Chen, Peizhe Sun
Summary: The significant increase in municipal solid waste (MSW) has become an important environmental issue. Pyrolysis, a thermal treatment process, shows promise in reducing MSW and generating valuable products. This study investigated the potential of carbon residue (CR) as an effective H2O2 activator. The CR was obtained from the co-pyrolysis of abandoned cartons and plastic wraps, which are increasingly prevalent in MSW.
ACS ES&T ENGINEERING
(2023)
Article
Engineering, Environmental
Binglin Guo, Junyue Wang, Krishnamoorthy Sathiyan, Xingmao Ma, Eric Lichtfouse, Ching-Hua Huang, Virender K. Sharma
Summary: Low levels of natural organic matter (NOM) enhance removal of trimethoprim and sulfamethoxazole by ferrate(VI) through the formation of more reactive Fe(V) species. The presence of antibiotics in water sources threatens public health and ecosystems. Nine types of NOM and NOM model compounds were found to improve the removal of trimethoprim and sulfamethoxazole by ferrate(VI) under mild alkaline conditions, possibly due to the presence of phenolic moieties in NOMs. The formation of Fe(V) through single-electron transfer from NOM to Fe(VI) generates NOM radicals within milliseconds, resulting in the enhanced removal of antibiotics despite concurrent reactions between Fe(V) and NOM moieties, radicals, and water. Humic and fulvic acids from lake and river waters also showed similar results, supporting the enhanced abatement of antibiotics in real water situations.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Hao Liu, Xinyang Li, Hong Yao, Peizhe Sun
Summary: This study investigated the mechanism of phosphate recovery by magnesium galvanic process (MGP) in synthetic and real urine matrixes. The results showed that the major components in urine (NH4+, Cl-, and HCO3-) all accelerated the corrosion of Mg plate, but with distinct underlying mechanisms. Pretreating urine with HCl significantly enhanced the performance of MGP and resulted in over 95% phosphate recovery in real urine.
Article
Environmental Sciences
Chengsong Ye, Kaiting Zhang, Peizhe Sun, Yunho Lee, Kyriakos Manoli, Xin Yu, Mingbao Feng
Summary: This study presents an advanced oxidation strategy using ruthenium(III) and periodate, which can rapidly eliminate microbial and chemical pollutants in water, including bacteria, bacteriophage MS2, antibiotic resistance genes, and organic micropollutants.
Article
Engineering, Environmental
Bing Wang, Yiwen Liu, Xin Wang, Peizhe Sun
Summary: This study explored the feasibility of using source-separated urine to overcome hydrogen loss in microbial electrolysis cells (MECs). The results showed that adding total ammonia nitrogen at optimal concentrations greatly improved hydrogen production rate and cathode recovery efficiency, while maintaining uninterrupted electroactivity in the anode.
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)