Denaturing reversed phase liquid chromatographic separation of non-coding ribonucleic acids on macro-porous polystyrene-divinylbenzene resins

The ability of denaturing ion-paired reversed phase LC to separate RNA was assessed using macro-porous polystyrene-divinylbenzene resins as the stationary phase. Using the three stationary phases with different pore size and a mobile phase containing phosphate, we separated RNAs of 20-8000 nucleotides with extremely high sensitivity, e.g., 50 pg for an RNA 20 nucleotides in length, S/N = 5. The method was used to separate non-coding RNAs obtained from biological sources and is suited for use with direct MS-based chemical characterization. (C) 2013 Published by Elsevier B.V.