Article
Environmental Sciences
Bianca do Amaral, Patricio Peralta-Zamora, Noemi Nagata
Summary: A simple and straightforward QuEChERS extraction method was proposed for the simultaneous determination of multiple pesticides in soil with high agricultural activity from southeastern Brazil. Experimental results showed the method's suitability for real soil samples and identified the optimal operational conditions for analyte recovery.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Multidisciplinary Sciences
Aya Roshdy, Heba Elmansi, Shereen Shalan, Amina El-Brashy
Summary: A new high performance liquid chromatography method was developed using a 2(3) full factorial design model to estimate three antifungal drugs simultaneously. The method demonstrated successful separation and estimation of fluconazole, itraconazole, and terbinafine with high sensitivity, providing an important reference for the treatment of severe fungal infections.
ROYAL SOCIETY OPEN SCIENCE
(2021)
Review
Chemistry, Physical
Riham K. Ahmed, Engy M. Saad, Hussein M. Fahmy, Rasha M. El Nashar
Summary: The design of experiments is crucial for optimizing operating conditions and obtaining adequate results, especially in the fields of pharmaceuticals, food, and environmental analysis. Electrochemical sensors, as fast and reliable tools, have wide applicability in various fields.
CURRENT OPINION IN ELECTROCHEMISTRY
(2022)
Article
Materials Science, Multidisciplinary
Majid Khodaei, Javad Moghaddam, Reza Ahmadi
Summary: Hydrometallurgy is an efficient process for recovering heavy metals. In this study, the optimal conditions for dissolving cobalt from zinc plant residues were determined using the Taguchi experiment design method. The results showed that the best conditions were an initial sulfuric acid concentration of 1 M, a temperature of 90 degrees C, a reaction time of 60 minutes, and a solid/liquid ratio of 1:7.
Article
Psychology, Multidisciplinary
Louis Laurencelle, Denis Cousineau
Summary: This article introduces a method called Arcsine transform, which can be used for analyzing proportions in any experimental design, including multiple groups, repeated measures, and factorial designs. This method is analogous to analysis of variance for continuous data and allows for the examination of interactions, main and simple effects, post-hoc tests, orthogonal contrasts, etc. The authors illustrate the method with several examples and explore type I error rates, power computation, and confidence intervals for proportions using Monte Carlo simulations.
FRONTIERS IN PSYCHOLOGY
(2023)
Article
Chemistry, Analytical
Aprilia Nur Tasfiyati, Lucia Dwi Antika, Rizna Triana Dewi, Abdi Wira Septama, Akhmad Sabarudin, Teni Ernawati
Summary: Scopoletin is a major constituent of noni fruits, contributing to antioxidative, anti-inflammatory, immunomodulatory, and hepatoprotective properties. The study proposes the use of accelerated solvent extraction (ASE) and HPLC-DAD for rapid identification and quantification of scopoletin. The established method provides a reliable and sensitive approach for determining scopoletin content in noni samples.
Article
Psychology, Mathematical
Sebastian Musslick, Anastasia Cherkaev, Ben Draut, Ahsan Sajjad Butt, Pierce Darragh, Vivek Srikumar, Matthew Flatt, Jonathan D. Cohen
Summary: Experimental design is crucial for reproducible research, but with increasing complexity, researchers struggle to measure variables of interest without confounds. SweetPea, an open-source declarative language, allows researchers to describe their experiment and sample sequences in an unbiased way, leveraging advancements in computer science. Its application extends to various empirical research domains, but current limitations and potential applications require further exploration.
BEHAVIOR RESEARCH METHODS
(2022)
Article
Energy & Fuels
Gokcen Gokceli, Sinem Bozar, Nilgun Karatepe
Summary: Experimental design plays an important role in reducing errors, saving materials and time costs, and interpreting results. Optimization of the absorber layer of inverted perovskite solar cells was carried out using a 2(3) factorial design. The results showed that the concentration of lead iodide had the most significant effect on efficiency, followed by the interaction between lead iodide-methylammonium iodide concentration and lead iodide concentration-spin speed. The sample prepared with the optimal conditions maintained 93.5% of its initial efficiency after 500 hours.
INTERNATIONAL JOURNAL OF ENERGY RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Vesna Milanovic, Federica Cardinali, Lucia Aquilanti, Antonietta Maoloni, Cristiana Garofalo, Matteo Zarantoniello, Ike Olivotto, Paola Riolo, Sara Ruschioni, Nunzio Isidoro, Matteo Cattalani, Gloriana Cardinaletti, Francesca Clementi, Andrea Osimani
Summary: The research revealed that using insects as feed can significantly impact the spread of antibiotic resistance genes in aquaculture, particularly in fish. This study provided initial insights into the spread of resistance genes in the food chain, laying down an important foundation for future assessments of antibiotic resistance risks.
ANIMAL FEED SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Applied
Fatma Doyuk, Kenan Dost
Summary: The hypothesis of this study aimed to extract six antibiotics from chicken breast meat belonging to four different classes using a developed extraction method, and determine their residues using an HPLC/DAD method. The validation data confirmed the achievement of this hypothesis. LC-MS/MS method was used to confirm the results. The developed HPLC/DAD method showed high linearity and satisfactory recoveries, accuracy, and precision.
Article
Plant Sciences
Zoe P. Morreeuw, David Castillo-Quiroz, Leopoldo J. Rios-Gonzalez, Raul Martinez-Rincon, Norma Estrada, Elda M. Melchor-Martinez, Hafiz M. N. Iqbal, Roberto Parra-Saldivar, Ana G. Reyes
Summary: The study compared the content of active compounds such as flavonoids in Agave lechuguilla waste biomass from different locations and evaluated the impact of extractive solvents and storage conditions on flavonoid recovery. Results showed variations in total polyphenolics content but similar total flavonoid content among regions; higher flavonoid content was found in ethanolic extracts, and flavonoids remained stable after sealed storage.
Article
Biochemistry & Molecular Biology
Marta Dabrowska, Eliana B. Souto, Izabela Nowak
Summary: The study successfully developed an optimized dispersion of lipid nanoparticles for incorporating hydrophilic active substances, including iridoid glycosides, with high encapsulation efficiency. Stability study revealed that room temperature is the most suitable condition for maintaining the expected physicochemical parameters.
Article
Energy & Fuels
Sayed Rashad, Ghadir El-Chaghaby, Eder C. Lima, Glaydson Simoes dos Reis
Summary: In this study, the ultrasonic-assisted extraction of antioxidants from Ulva lactuca was optimized using a full-factorial design. The results showed that temperature and solvent were the most influential factors on the extraction process. The optimum extraction conditions were 1 hour extraction time, 50% ethanol as solvent, and 25 degrees C temperature.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Chemistry, Multidisciplinary
Malgorzata Artymowicz, Szymon Macioszek, Julia Jacyna, Joanna Dawidowska, Wiktoria Struck-Lewicka, Micha l J. Markuszewski, Danuta Siluk
Summary: This study aimed to develop a sample preparation procedure for the simultaneous extraction of 12 selected modified nucleosides and deoxynucleosides using solid phase extraction (SPE) technique. The developed and optimized SPE procedure allowed for successful extraction with a mean recovery of 76.0%, and was applied to urine samples using high performance liquid chromatography coupled with triple quadrupole mass spectrometry (HPLC-QqQ/MS).
SUSTAINABLE CHEMISTRY AND PHARMACY
(2021)
Article
Engineering, Environmental
Saran Saensook, Akawat Sirisuk
Summary: Black titanium dioxide was synthesized through a chemical reduction method and showed high efficiency in degrading methyl orange under both UV and visible light irradiation. The study found that calcination temperature, calcination time, and the molar ratio of NaBH4 to TiO2 had significant impacts on the photocatalytic activity. Black titanium dioxide with abundant surface defects acted as an electron trap and inhibited electron-hole recombination.
JOURNAL OF WATER PROCESS ENGINEERING
(2022)
Article
Biochemical Research Methods
Shama Naz, Angela Algaba Calderon, Antonia Garcia, Jessica Gallafrio, Rodrigo Teijeiro Mestre, Elena Gonzalez Gonzalez, Carlos Munoz de Cabo, Mari Cruz Martin Delgado, Jose Angel Lorente Balanza, Ana Valeria Colnaghi Simionato, Nicolas Nin Vaeza, Coral Barbas, Francisco J. Ruperez
Article
Biochemical Research Methods
Adriana Zardini Buzatto, Sumaya Ferreira Guedes, Mariana de Oliveira Silva, Jessica Mirela Gallafrio, Ana Valeria Colnaghi Simionato
Article
Biochemical Research Methods
Heloisa Moretti Passos, Zuzana Cieslarova, Ana Valeria Colnaghi Simionato
Article
Biochemical Research Methods
Eduardo Adilson Orlando, Aline Gabriela Costa Roque, Marcos Eliseu Losekann, Ana Valeria Colnaghi Simionato
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2016)
Article
Chemistry, Analytical
Zuzana Cieslarova, Fernando Silva Lopes, Claudimir Lucio do Lago, Marcondes Cavalcante Franca, Ana Valeria Colnaghi Simionato
Article
Food Science & Technology
Mari Uyeda, Gustavo Henrique Bueno Duarte, Anna Maria Alves de Piloto Fernandes, Fabiano Jares Contesini, Marcia Cristina Fernandes Messias, Gabrielle Kristine Doratiotto de Santis, Karina Vieira Barros, Ana Valeria Colnaghi Simionato, Patricia de Oliveira Carvalho
FOOD RESEARCH INTERNATIONAL
(2020)
Article
Chemistry, Analytical
Adriana Teixeira Godoy, Marcos Nogueira Eberlin, Ana Valeria Colnaghi Simionato
Article
Biochemistry & Molecular Biology
Anna Maria A. P. Fernandes, Marcia C. F. Messias, Gustavo H. B. Duarte, Gabrielle K. D. de Santis, Giovana C. Mecatti, Andreia M. Porcari, Michael Murgu, Ana Valeria C. Simionato, Thalita Rocha, Carlos A. R. Martinez, Patricia O. Carvalho
Article
Biochemical Research Methods
Gustavo Henrique Bueno Duarte, Anna Maria Alves de Piloto Fernandes, Alex Aparecido Rosini Silva, Hans R. Zamora-Obando, Alan Goncalves Amaral, Alessandra de Sousa Mesquita, Jayr Schmidt-Filho, Vladmir C. Cordeiro de Lima, Felipe D'Almeida Costa, Victor Piana Andrade, Andreia M. Porcari, Marcos Nogueira Eberlin, Ana Valeria Colnaghi Simionato
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2020)
Review
Biochemistry & Molecular Biology
Regina V. Oliveira, Ana Valeria C. Simionato, Quezia B. Cass
Summary: Metabolomics and lipidomics play a crucial role in understanding disease mechanisms and physiological processes. Chirality analysis is essential for studying the physiological functions of bioactive molecules, but there are challenges in this stereoselective analysis.
Article
Biochemical Research Methods
Adriana Zardini Buzatto, Mariana de Oliveira Silva, Ronei Jesus Poppi, Ana Valeria Colnaghi Simionato
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2017)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)