Article
Chemistry, Analytical
Luxiu Li, Ya Cui, Lingling Ren, Chunyang Li, Yehao Liu, Zhongxiu Jin
Summary: A simple and efficient dispersive solid-phase microextraction method based on magnetic dummy molecularly imprinted polymer nanoparticles was developed for the extraction of five phthalate monoesters from human urine samples. The prepared nanoparticles showed high selectivity, good adsorption capacity, excellent reusability, and fast adsorption rates. Furthermore, the method demonstrated good linearity and recovery rates for all five phthalate monoesters in human urine samples.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Zhuang-Fei Jiang, Qin Li, Qing-Yao Li, Hui-Xian Xu, Jia-Yuan He, Chong-Zhi Wang, Lian-Di Zhou, Qi-Hui Zhang, Ling Luo, Chun-Su Yuan
Summary: A new adsorbent was synthesized using dummy-template molecular imprinting technology, magnetic separation technology, and pH-responsive technology. This adsorbent can quickly enrich anthocyanins in fruit and detect them using electrochemical methods. The results showed that the dummy-template magnetic and pH-responsive molecularly imprinted polymers had shorter equilibrium time, higher selectivity, and better adsorption capacity compared to structural analogues.
MICROCHEMICAL JOURNAL
(2022)
Article
Environmental Sciences
Shikha Bhogal, Kuldeep Kaur, Irshad Mohiuddin, Sandeep Kumar, Jechan Lee, Richard J. C. Brown, Ki-Hyun Kim, Ashok Kumar Malik
Summary: HPMIPs are promising adsorbents with advantageous properties, allowing for improved binding capacity and kinetics, particularly efficient in extracting trace-level targets from complex matrices.
ENVIRONMENTAL POLLUTION
(2021)
Article
Biochemical Research Methods
Zilong Chen, Hongdong Liu, Jie Chen, Weifeng Zhu, Yong Liu, Jianxin Min, Xiaotian Chen, Bin Li, Xin Yang
Summary: In this study, a novel strategy utilizing dummy molecular imprinting technology and magnetic separation technology was developed for the separation and enrichment of sibiskoside. The prepared dummy template molecularly imprinted polymers (DMIPs) demonstrated high adsorption capacity for sibiskoside and good reusability. The purity of sibiskoside in crude extracts was increased to 78%, providing a foundation for the further development and utilization of Sibiraea angustata and the separation and enrichment of monoterpenes.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Chemistry, Applied
Nilawan Surapong, Prachathipat Pongpinyo, Yanawath Santaladchaiyakit, Rodjana Burakham
Summary: An eco-friendly magnetic dual-dummy-template molecularly imprinted polymer (MDDMIP) was synthesized using mixed-valence iron hydroxide, a deep eutectic solvent, and caffeic acid and glutamic acid as binary monomers. The MDDMIP exhibited high adsorption capacities, quick adsorption times, and high imprinting factors for organophosphorus pesticides (OPPs). The MDDMIP was utilized for magnetic solid phase extraction combined with high performance liquid chromatography (HPLC) to detect OPPs in food samples.
Article
Chemistry, Analytical
Ling-Tan, Li-Li Yang, Yan-Jun Li, Zhuang-Fei Jiang, Qing-Yao Li, Rong-Rong Ma, Jia-Yuan He, Lian-Di Zhou, Qi-Hui Zhang, Chun-Su Yuan
Summary: Two distinct new dummy-template molecularly imprinted polymers (D-MIPs) were prepared for selective extraction of paclitaxel (PTX) using different template molecules, exhibiting high adsorption capacity and selectivity. Both 10-DAB-MIPs and SC-MIPs could effectively enrich PTX from structural analogs and act as drug carriers in complex biological systems. These D-MIPs have great potential for adsorption materials for PTX and drug delivery systems.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Anna Herrera-Chacon, Andreu Gonzalez-Calabuig, Manel del Valle
Summary: This study introduces a voltammetric sensor based on a dummy molecularly imprinted polymer (MIP) for the detection of 2,4,6-trinitrotoluene (TNT) and 2,4-dinitrophenol (DNP). The sensor is cost-effective, rapid, and simple to use. Chemometric tools, particularly principal component analysis (PCA), demonstrate the sensor's potential applications in environmental settings for discriminating nitroaromatic and potential interfering species.
Article
Biochemical Research Methods
Xiaoya Fu, Xia Wang, Zhining Xia, Yike Huang
Summary: In this study, a sample pretreatment method for genotoxic impurities (GTIs) with very trace content in medicine was established using dummy template molecular imprinting technology (DMIT) and solid phase extraction. The optimal adsorption conditions were obtained through conditional optimization, and the quality control of GTIs in pharmaceuticals was achieved.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Yang Cheng, Hongdi Liu, Jing Li, Lixue Kuang, Guofeng Xu
Summary: In this study, magnetic molecularly imprinted polymers (Fe3O4@MIPs) with excellent selectivity and high adsorption capacity were successfully synthesized and applied for the detection of chlorogenic acid (CGA) in juice samples, achieving satisfactory results.
MICROCHEMICAL JOURNAL
(2022)
Article
Biochemical Research Methods
Lei Gao, Dongli Qin, Zhongxiang Chen, Song Wu, Shizhan Tang, Peng Wang
Summary: A novel purification method using MCNTMIP nanocomposite for separation and enrichment of SAs in fish and shrimp samples was developed successfully in this study. The method offers advantages of simple magnetic separation, specific molecular recognition, and high adsorption capacity.
Article
Food Science & Technology
Xiaolin Cao, Zheng Zhang, Guangyang Liu, Ziping Zhang, Jungang Yin
Summary: Efficient magnetic dummy molecular imprinted polymers were synthesized for the first time using a dummy template and characterized for their selective recognition capacity towards aminoglycoside antibiotics. A method for enriching and determining the target analytes in milk samples was developed using magnetic solid-phase extraction coupled with high performance liquid chromatography-tandem mass spectrometry, showing good sensitivity and repeatability.
FOOD ANALYTICAL METHODS
(2021)
Article
Biochemical Research Methods
Dongqin Tan, Chang Han, Zhonglin Yu, Xiaoli Sun, Jingwu Chen, Dhanjai, Xueting Shao, Degao Wang
Summary: A molecularly imprinted polymer was constructed for the first time using the dummy imprinting strategy with homopiperonylamine as the dummy template. The polymer showed high selectivity towards amphetamine-type stimulants and could rapidly adsorb them from water. It also exhibited low matrix effects and could be used for the pretreatment of these stimulants in alcoholic and nonalcoholic beverages.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Review
Biochemical Research Methods
J. Marfa, R. R. Pupin, M. P. T. Sotomayor, M. I. Pividori
Summary: Magnetic particles and molecularly imprinted polymers have been widely utilized in scientific and technological applications, revolutionizing separation and bioanalytical methodologies; Hybrid molecularly imprinted polymers offer a cheaper and more stable alternative to biologically modified magnetic particles; The direct detection of electroactive analytes based on magnetic-MIP preconcentration shows potential in various fields such as food safety and environmental monitoring.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Applied
Roseane Andrade Teixeira, Laise Aparecida Fonseca Dinali, Hanna Leijoto de Oliveira, Anny Talita Maria da Silva, Keyller Bastos Borges
Summary: A magnetic mesoporous molecularly imprinted polymer was successfully synthesized and applied in magnetic solid phase extraction for selective determination of organophosphorus pesticides in mineral water and grape samples; after sample preparation optimization, recoveries of 99.56% and 98.86% were obtained for azamethiphos and chlorpyrifos.
Review
Chemistry, Analytical
Yahan Cui, Lan Ding, Jie Ding
Summary: Magnetic imprinted polymers (MMIPs) are synthetic polymeric materials that have wide-ranging applications in sample processing. By combining magnetic separation technology, MMIPs not only have high selectivity for adsorbing target analytes, but also provide convenience for rapid separation and processing of large sample volumes. This review summarizes recent advanced preparation strategies of MMIPs and highlights their applications in complex samples.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yusi Bu, Xiaolin Zhang, Aihong Zhu, Linhao Li, Xiaoyu Xie, Sicen Wang
Summary: Inside-out cell membrane-coated nanoparticles were prepared for screening tyrosine kinase inhibitors and successfully targeted potential antitumor compounds from lung adenocarcinoma. This novel approach enhances drug screening efficiency and broadens possibilities for drug lead discovery in the future.
ANALYTICAL CHEMISTRY
(2021)
Article
Plant Sciences
Xiao-Yu Xie, Yi-Xuan Yang, Xin Gao, Qian Liu, Zi-Han Liu, Xiao-Feng Niu, Xia Liu
Summary: New dinor-eudesmane sesquiterpenoids were isolated from the root extract of Chloranthus multistachys Pei, and their structures were elucidated through spectroscopic analysis. The cytotoxicity activities of the compounds against selected cancer cells were evaluated through MTT assay.
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
(2022)
Article
Chemistry, Analytical
Jing Li, Yixuan Yang, Aihong Zhu, Lujun Li, Xia Liu, Xiaoyu Xie
Summary: Highly specific novel glycopeptide-based fluorescent molecularly imprinting polymers (g-FMIPs) were constructed for recognizing and determining target glycoproteins in complex biological samples. The g-FMIPs demonstrated rapid binding kinetics, high adsorption capacity, remarkable selectivity, and sensitivity, offering potential prospects for precision recognition and sensitive detection of trace glycoproteins from complex biosamples.
Article
Pharmacology & Pharmacy
Qi Hu, Lanlan Jia, Xiaolin Zhang, Aihong Zhu, Sicen Wang, Xiaoyu Xie
Summary: A high-performance cell membrane biomimetic graphene nanodecoy was developed by purposeful surface engineering, improving stability and screening efficiency for active components of traditional Chinese medicine. The camouflaged nanodecoy showed satisfactory adsorption capacity and selectivity through interaction with specific ligands. Active components screened from Angelica dahurica exhibited potential antiproliferative activity validated by pharmacological studies.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Chemistry, Multidisciplinary
Xueyan Zhen, Linhao Li, Lanlan Jia, Aihong Zhu, Yixuan Yang, Sicen Wang, Xiaoyu Xie
Summary: Researchers developed an erythrocyte membrane-cloaked chemotherapeutic nanomissile delivery system modified with an anti-EGFR aptamer. This system achieved precise tumor targeting, cell apoptosis induction, and synergistic therapy by suppressing EGFR expression levels and delivering the drug. It exhibited excellent drug retention and tumor inhibition abilities both in vitro and in vivo.
CHINESE CHEMICAL LETTERS
(2023)
Article
Pharmacology & Pharmacy
Xiaolin Zhang, Xueyan Zhen, Yixuan Yang, Quan Feng, Wanqing Yuan, Xiaoyu Xie
Summary: Cell membrane camouflaged nanoparticles have been widely used due to their unique biointerface targeting function in drug leads discovery. However, random orientation of the cell membrane coating may not guarantee effective binding of drugs to specific sites, especially in intracellular regions. Bioorthogonal reactions offer a specific and reliable method for cell membrane functionalization without disrupting the living biosystem. In this study, inside-out cell membrane camouflaged magnetic nanoparticles (IOCMMNPs) were constructed via bioorthogonal reactions to screen small molecule inhibitors targeting intracellular tyrosine kinase domain of vascular endothelial growth factor receptor-2.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Biophysics
Xiaolin Zhang, Lanlan Jia, Nanxin Liu, Ying Zhao, Tingting Zhang, Xiaoyu Xie
Summary: The use of inside-out oriented extracellular vesicles coated magnetic nanoparticles enables the discovery of low-toxicity and high-efficiency P-gp inhibitors from natural products. This method provides an effective tool for the screening of novel P-gp inhibitors, expanding the application field of cell membrane biomimetic magnetic nanoparticles.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Chemistry, Physical
Lanlan Jia, Xueyan Zhen, Liang Chen, Quan Feng, Wanqing Yuan, Yusi Bu, Sicen Wang, Xiaoyu Xie
Summary: A biomimetic approach based on the conjugation of multivalent aptamer onto tumor cell membranecoated magnetic graphene oxide has been developed for efficient and sensitive circulating tumor cells (CTCs) detection. This approach overcomes the limitations of current CTCs detection techniques and shows promising potential for clinical cancer diagnostics.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Materials Science, Biomaterials
Qi Hu, Lanlan Jia, Peishan Li, Tingting Zhang, Quzha Silang, Xiaoyu Xie, Sicen Wang
Summary: Efficient integration of cell membrane-nanomaterial hybrids (CN hybrids) is crucial in bioanalysis. However, the poor dispersity of nanomaterials hinders its development. A method was proposed to improve integration efficiency by establishing a dynamic dispersion system to enhance the interaction between cell membranes and nanomaterials. The addition of dextran as a macromolecular stabilizer improved the dispersity of the nanomaterials and avoided resistance to membrane coating. The design principle provided a new perspective for optimizing the behavior of CN hybrids and enhancing their effectiveness in bioanalytical applications.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Chemistry, Multidisciplinary
Lanlan Jia, Qi Hu, Tingting Zhang, Zhaojia Wang, Dan Wu, Xiaoyu Xie, Sicen Wang
Summary: A biomimetic biosensor is designed in this study, which integrates dual-targeting multivalent aptamer/walker duplex functionalized biomimetic magnetic beads and an enzyme-powered DNA walker signal amplification strategy. This biosensor enables efficient and high purity enrichment of heterogeneous circulating tumor cells (CTCs) with different epithelial cell adhesion molecule (EpCAM) expression, while minimizing leukocyte interference. This work provides a new perspective and paves the way for early cancer diagnosis.
Article
Chemistry, Physical
Xueyan Zhen, Lanlan Jia, Qingyu Tang, Ying Zhao, Peishan Li, Jing Li, Xiaoyu Xie, Sicen Wang
Summary: We developed a hybrid engineered biointerface nanoplatform that integrates anti-EGFR-aptamer modified liposomes with tumor cell membranes to overcome the limitations of PDT in tumor selectivity and hypoxic tumor microenvironment.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Nanoscience & Nanotechnology
Yusi Bu, Dan Wu, Ying Zhao, Guoxiang Wang, Xintao Dang, Xiaoyu Xie, Sicen Wang
Summary: Cell membrane coating technology can enhance the surface density of membrane receptors and exhibit exceptional sensitivity. In this study, high density FGFR4 cell membrane-coated nanoparticles were successfully fabricated through covalent site-specific immobilization, and the enhanced receptor density was confirmed.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Physical
Ruifang Ke, Xueyan Zhen, Huai-Song Wang, Linhao Li, Hongying Wang, Sicen Wang, Xiaoyu Xie
Summary: A multifunctional bioreactor was developed by embedding glucose oxidase and doxorubicin in tumor targeting ligand modified red blood cell membrane camouflaged metal-organic framework nanoparticles. This bioreactor achieved synergistic starvation therapy and chemotherapy.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Materials Science, Biomaterials
Qi Hu, Xiaolin Zhang, Lanlan Jia, Xueyan Zhen, Xiaoyan Pan, Xiaoyu Xie, Sicen Wang
BIOMATERIALS SCIENCE
(2020)
Article
Materials Science, Biomaterials
Yusi Bu, Qi Hu, Xiaolin Zhang, Ting Li, Xiaoyu Xie, Sicen Wang
BIOMATERIALS SCIENCE
(2020)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)