期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1279, 期 -, 页码 98-107出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2013.01.020
关键词
Methylation; Polar lipid; Supercritical fluid chromatography; Tandem mass spectrometry
资金
- SUNTORY Institute for Bioorganic Research
- [23686120]
- Grants-in-Aid for Scientific Research [23686120] Funding Source: KAKEN
Supercritical fluid chromatography/tandem mass spectrometry (SFC/MS/MS) with methylation was used for the simultaneous profiling of diverse polar lipids in a mixture. A high throughput, high resolution analysis of nineteen classes of polar lipids including phospholipids, lysophospholipids, and sphingolipids was performed in 6 min. Methylation by trimethylsilyl-diazomethane suppressed peak tailing and improved detection sensitivity of phosphatidylserine (PS), phosphatidic acid (PA), lysophosphatidylserine (LPS), lysophosphatidylinositol (LPI), lysophosphatidic acid (LPA), ceramide-l-phosphate (Cer1P), sphingosine-1-phosphate (Sol P), and sphinganine-I-phosphate (Sal P). The limits of detection for PS, PA, LPS, LPI, LPA, Cer1P. Sol P. and Sal P were enhanced 7.5-, 26.7-, 600-, 116.7-, 500-, 75-, 3000-, and 4500-fold, respectively. Global qualitative and quantitative analysis of not only the high-abundance species but also the low-abundance species in the polar lipids was achieved. When the method was applied to mouse liver, 4 PSs, 24 PAs, 3 lysophosphatidylethanolamines, 11 LPSs, 6 lysophosphatidylglycerols, 4 LPIs, 13 LPAs, 7 sphingomyelins, 11 Cer1Ps, Sol P, and Sal P were additionally analyzed. Furthermore, the quantification of various molecular species in each polar lipid was carried out. (C) 2013 Elsevier B.V. All rights reserved.
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