Article
Chemistry, Analytical
Delphine Zanella, Adele Henin, Steven Mascrez, Pierre-Hugues Stefanuto, Flavio Antonio Franchina, Jean-Francois Focant, Giorgia Purcaro
Summary: The potential of exhaled breath for disease diagnosis is significant, but standardized sampling and analysis procedures are necessary for reliable results. This study validates the performance of two different comprehensive two-dimensional gas chromatography coupled to mass spectrometry platforms in different laboratories, using the Peppermint Initiative guidelines. The platforms successfully tracked the peppermint oil metabolites in exhaled breath over a 6.5-hour period.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Ronja Weber, Bettina Streckenbach, Lara Welti, Demet Inci, Malcolm Kohler, Nathan Perkins, Renato Zenobi, Srdjan Micic, Alexander Moeller
Summary: Breath analysis can non-invasively assess altered metabolism and disease-associated processes, providing a potential solution to improve the diagnosis and management of pediatric asthma. In this study, exhaled metabolic signatures were identified using SESI/HRMS, allowing for the distinguishing of children with allergic asthma from healthy controls.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Chemistry, Analytical
Zhenzhen Xie, James D. Morris, Stephanie J. Mattingly, Saurin R. Sutaria, Jiapeng Huang, Michael H. Nantz, Xiao-An Fu
Summary: Analysis of volatile organic compounds (VOCs) in exhaled breath has great potential for disease detection, but the analysis of metabolic VOCs is still challenging. This study presents a method combining ultrahigh-performance liquid chromatography with high-resolution mass spectrometry for analyzing carbonyl metabolites in breath. A silicon microreactor was used to capture carbonyl compounds in exhaled breath, and six subgroups of carbonyl compounds were identified. This method may provide a quantitative approach for identifying disease markers in exhaled breath.
ANALYTICAL CHEMISTRY
(2023)
Review
Medical Laboratory Technology
Ting Chen, Tiannan Liu, Ting Li, Hang Zhao, Qianming Chen
Summary: The review introduces the conventional and emerging methods for breath analysis in diagnosing and monitoring various diseases. It discusses the correlation between breath components and specific diseases, as well as unique ideas and devices for the diagnosis of common diseases through exhaled breath analysis. The potential application of exhaled breath analysis for diagnosing and screening different diseases is briefly introduced, offering a new avenue for non-invasive disease detection.
CLINICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Qing Wen, Antonis Myridakis, Piers R. Boshier, Simone Zuffa, Ilaria Belluomo, Aaron G. Parker, Sung -Tong Chin, Stephanie Hakim, Sheraz R. Markar, George B. Hanna
Summary: Volatolomics provides a noninvasive approach for detecting and monitoring human diseases. In this study, we developed a complete pipeline for untargeted urinary volatolomic profiling, optimized the extraction technique, improved the volatolome coverage, and applied strict quality control measures. The pipeline was successfully used to discriminate pancreatic ductal adenocarcinoma patients from control subjects.
ANALYTICAL CHEMISTRY
(2023)
Article
Allergy
Sylvia Kreissl, Sarah Hendler, Manas K. Akmatov, Marie von Meien, Ursula Range, Frank Pessler, Christian Vogelberg
Summary: The study showed that preschool dEBC pH combined with CAP class may serve as a highly sensitive, noninvasive marker for the early detection of young asymptomatic children with increased asthma risk. The need for additional biomarkers with high specificity is also emphasized to optimize early risk stratification in this challenging clinical scenario.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY-IN PRACTICE
(2021)
Article
Chemistry, Analytical
Liu Yi-jun, Qian Yang-yang, Shu Bing, Liu Yang-yang, Tu Xing-hao, Ouyang Hong-jun, Li Yahui, Tan Ge, Yu Zi-wei, Chen Fei, Lin Li-jing
Summary: The study compared the effects of four different drying methods on the volatile organic compounds in Ganoderma lucidum, revealing that the types and contents of VOCs were influenced by the specific drying method used. Heating processes increased the content of certain compounds while decreasing others, with specific compounds remaining identifiable throughout the drying process.
MICROCHEMICAL JOURNAL
(2021)
Article
Cell Biology
Wadah Ibrahim, Michael J. Wilde, Rebecca L. Cordell, Matthew Richardson, Dahlia Salman, Robert C. Free, Bo Zhao, Amisha Singapuri, Beverley Hargadon, Erol A. Gaillard, Toru Suzuki, Leong L. Ng, Tim Coats, Paul Thomas, Paul S. Monks, Christopher E. Brightling, Neil J. Greening, Salman Siddiqui, EMBER Consortium
Summary: Acute cardiorespiratory breathlessness is a significant cause of emergency hospitalizations. This study identified breath volatile organic compound biomarkers that can diagnose acute cardiorespiratory disease and investigated the metabolic enrichment patterns in acute disease. The findings demonstrate that the multibiomarker score can effectively differentiate acute disease from health and different subtypes of acute cardiorespiratory exacerbation. Furthermore, the study revealed specific enrichment patterns in different subgroups of acute disease.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Hunter R. Yamanaka, Cynthia Cheung, Jireh S. Mendoza, Danson J. Oliva, Kealina Elzey-Aberilla, Katelynn A. Perrault
Summary: Fast diagnostic results using breath analysis are a potential future direction for disease diagnosis and health screenings;Testing that does not require sending specimens to medical laboratories has advantages;Baseline breath VOC analysis of control populations is crucial for future research.
Article
Medicine, General & Internal
Michiel A. G. E. Bannier, Sophie Kienhorst, Quirijn Jobsis, Kim D. G. van de Kant, Frederik-Jan Van Schooten, Agnieszka Smolinska, Edward Dompeling
Summary: Exhaled breath analysis has the potential to diagnose various respiratory and non-respiratory diseases. This study found that inhaled corticosteroids can influence the volatile organic compounds in the exhaled breath of wheezing preschool children. Furthermore, the exhaled volatile organic compounds can predict the response to corticosteroid treatment in these children.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Chemistry, Analytical
Shakiba Zeinali, Chiranjit Ghosh, Janusz Pawliszyn
Summary: Breath-composition analysis is a non-invasive method for early disease diagnosis and exposure history investigation. To overcome the limitations of aerosol nature and low concentrations of volatile organic compounds in breath samples, researchers applied a needle-trap device and electrospun polyacrylonitrile filter for breath characterization. The results showed that the needle-trap device had advantages in capturing droplets and extracting free gas-phase components.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Wan Sin Heng, Snehal R. Jadhav, Maiken Ueland, Robert A. Shellie
Summary: A new approach using static headspace-comprehensive two-dimensional gas chromatography (HS-GC x GC) with backflushing is introduced for rapid and reliable bacteria detection in food. This method allows fast detection of Escherichia coli (E. coli) in enriched ultra-high-temperature processed dairy milk by detecting microbial volatile organic compounds. The approach reduces the time-to-response by approximately one full day compared to conventional culture-based methods and can detect single-cell initial bacterial load.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Zhenzhen Xie, James D. Morris, Stephanie J. Mattingly, Saurin R. Sutaria, Jiapeng Huang, Michael H. Nantz, Xiao-An Fu
Summary: Analysis of volatile organic compounds (VOCs) in exhaled breath has shown great potential for disease detection. However, analyzing metabolic VOCs in breath is still a challenge. This study presents a method using ultrahigh-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-MS) to analyze a broad range of carbonyl metabolites in breath. The combination of a silicon microreactor for capturing carbonyl compounds and UHPLC-MS analysis may provide a quantitative method for identifying disease markers in exhaled breath.
ANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Rosaria Cozzolino, Matteo Stocchero, Rosa Perestrelo, Jose S. Camara
Summary: The volatile profiles of eight saffron samples grown in different geographical districts were studied using HS-SPME/GC-MS. Several key odorants and pharmacologically active metabolites were identified, and the samples were successfully differentiated based on variety using multivariate statistical analysis.
Article
Biochemical Research Methods
Xiaoxia Gong, Songyue Shi, Dong Zhang, Gerardo Gamez
Summary: Breath analysis has great potential for early disease diagnostics and clinical drug monitoring, but current mass spectrometer accessibility limitations restrict its point-of-care applications. Researchers have developed an offLine method for quantitative analysis of exhaled breath using low-temperature plasma-mass spectrometry, with optimized conditions showing competitive or significantly better detection limits compared to relevant techniques.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Cardiac & Cardiovascular Systems
Mariana R. Silva, Gualter S. Silva, Sara Fernandes, Joao Almeida, Paulo Fonseca, Marco Oliveira, Helena Goncalves, Francisca Saraiva, Antonio S. Barros, Pedro G. Teixeira, Ricardo L. Lopes, Francisco Sampaio, Silvia O. Diaz, Joao Primo, Ricardo Fontes-Carvalho
Summary: This study aimed to determine the predictors of early recurrence (ER) and late recurrence (LR) after catheter ablation (CA) for atrial fibrillation (AF) and establish the risk of LR in patients who experience ER. The results showed that the risk of LR after CA was significantly higher in patients with ER (five-fold increased risk). These findings highlight the importance of clarifying the clinical role of the blanking period.
JOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Ines Cruz, Sara Lopes Fernandes, Silvia O. Diaz, Francisca Saraiva, Antonio S. Barros, Joao Primo, Francisco Sampaio, Ricardo Ladeiras-Lopes, Ricardo Fontes-Carvalho
Summary: There is no independent association between epicardial adipose tissue (EAT) volume and attenuation and the risk of atrial fibrillation (AF) recurrence after AF ablation.
REVISTA ESPANOLA DE CARDIOLOGIA
(2023)
Article
Food Science & Technology
Serkan Selli, Rosa Perestrelo, Hasim Kelebek, Onur Sevindik, Fabiano Travaglia, Jean Daniel Coisson, Jose S. Camara, Matteo Bordiga
Summary: The impact of Japanese beetles on the quality indicators of Nebbiolo and Erbaluce grapes, specifically their phenolic and volatile composition, was examined. The study revealed that grapes produced by plants attacked by Japanese beetles had higher levels of phenolic compounds compared to healthy plants. Similarly, the volatile composition of the affected grapes was also higher. The plants responded to the beetle attack by increasing the content of certain volatile compounds.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Oncology
Fernando Osorio, Antonio S. Barros, Barbara Peleteiro, Isabel Amendoeira, Jose Luis Fougo
Summary: In this study, we compared the outcomes of breast cancer among three age groups and found that older patients were more susceptible to suboptimal treatment and had a lower overall survival rate. This was not associated with the cancer itself, but rather with undertreatment. The study suggests that tumor characteristics and treatment compliance are better predictors of survival than chronological age.
Article
Cardiac & Cardiovascular Systems
Marta Leite, Francisco Sampaio, Francisca A. Saraiva, Silvia O. Diaz, Antonio S. Barros, Ricardo Fontes-Carvalho
Summary: Recently, HF guidelines have re-classified HF patients with LVEF between 41% and 49% as HFmrEF. The treatment of HFmrEF patients is often considered a grey zone due to the lack of RCTs exclusively conducted on this population. A network meta-analysis was conducted to compare the treatment effect of different medications in HFmrEF cardiovascular outcomes.
Article
Food Science & Technology
Laura Alti-Palacios, Juana Martinez, Jose A. C. Teixeira, Jose S. Camara, Rosa Perestrelo
Summary: This study aimed to investigate the impact of cold pre-fermentative maceration (CPM) treatment on the aromatic and sensory properties of white wines from four grape varieties. A total of 62 aroma compounds were identified using HS-SPME/GC-MS. CPM treatment increased the relative concentration of alcohols, esters, and acids, while the impact on terpenoids varied with grape variety and vintage. Esters made the most significant contribution to the sensory properties, enhancing floral and fruity notes.
Article
Food Science & Technology
Goncalo Jasmins, Rosa Perestrelo, Jean Daniel Coisson, Patricia Sousa, Jose A. Teixeira, Matteo Bordiga, Jose S. Camara
Summary: A study aimed to identify characteristic molecular markers related to the authenticity of Italian fortified wines. The volatilomic fingerprint of popular Italian fortified wines was established using HS-SPME/GCMS. The identified compounds can be used as potential markers for the authenticity and genuineness of these wines, contributing to the identification of fraud or adulteration cases and ensuring quality and safety for consumers.
Article
Chemistry, Analytical
Jose S. Camara, Paulo Fernandes, Nelson Barros, Rosa Perestrelo
Summary: An accurate and selective method combining modified mu-QuEChERS procedure with LC-QqQMS was developed for quantification of PAT in commercial apple juices. This method reduced interference from the matrix and provided a quick analysis within 10 minutes. The method showed good linearity and high recovery for determination of PAT in apple juice.
Article
Chemistry, Analytical
Patricia Sousa, Irene Camacho, Jose S. Camara, Rosa Perestrelo
Summary: Researchers have developed a potential diagnostic method for breast cancer using SDS-PAGE and MALDI-TOF MS, which can distinguish between breast cancer patients and healthy individuals based on peptide ion biosignatures.
Article
Multidisciplinary Sciences
Laura Garcia-Cansino, Maria Angeles Garcia, Maria Luisa Marina, Jose S. Camara, Jorge A. M. Pereira
Summary: Food contamination with pesticides poses risks to consumer safety and undermines food supply chain confidence. This study compares and validates two microextraction techniques for extracting eight pesticides from wastewater samples. Both techniques show good analytical performance with selectivity, linearity, low LODs and LOQs, precision, and high recovery rates. The developed methodologies are simpler, faster, and have a lower environmental impact. The mu SPEed approach is found to be more efficient, easier to perform, and greener. Overall, this study highlights the potential of microextraction techniques for pesticide residue analysis and presents a fast and efficient method for pesticide analysis in wastewater samples.
Article
Allergy
Ana Margarida Pereira, Mariana Pereira, Luis Miguel Araujo, Maria Graca Castel-Branco, Ana Morete, Joao Almeida Fonseca
Summary: This study investigated the prevalence of self-reported adverse reactions (AdR) to subcutaneous airborne allergen immunotherapy (SCIT) in a Portuguese allergy unit. It found that adverse reactions were reported in 1/3 of the administrations, with most occurring at the injection site. Female gender and asthma were associated with a higher risk of adverse reactions, while SCIT preparations with multiple allergens and no dilutional effect had a lower risk.
EUROPEAN ANNALS OF ALLERGY AND CLINICAL IMMUNOLOGY
(2023)
Review
Engineering, Chemical
Cristina V. Berenguer, Rosa Perestrelo, Jorge A. M. Pereira, Jose S. Camara
Summary: Sustainable biomass production has great potential for reducing greenhouse gas emissions and providing alternative energy sources. Portugal's abundant residual biomass resources can be transformed into high-value products through thermochemical conversion technologies, improving economic performance, reducing reliance on fossil fuels, and achieving sustainable waste management.
Review
Microbiology
Rita Almeida Teixeira, Claudia Silva, Antonio Carlos Ferreira, Diana Martins, Adelino Leite-Moreira, Isabel M. Miranda, Antonio S. Barros
Summary: Gestational diabetes, affecting 10% of pregnancies, has implications for both mother and child. This study reviewed the literature on the differences in microbiota composition between pregnant women with gestational diabetes and healthy pregnancies. The findings suggest that certain bacteria are more abundant in healthy pregnancies, while others are more abundant in gestational diabetes. The study also highlighted the potential of microbiome-targeted therapies for gestational diabetes.
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)