Article
Chemistry, Applied
Zhenhui Ren, Yinhui Chen, Jianyi Wang, Pimiao Zheng, Huixia Zhang, Haiyang Jiang
Summary: A highly specific and sensitive icELISA method was developed to detect Brombuterol in animal feed and pork samples, with results showing good correlation with liquid chromatography-tandem mass spectrometry, demonstrating its potential for improving consumer protection in the area of food safety.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2021)
Article
Food Science & Technology
Xuan Zeng, Yuying Zheng, Yan He, Wei Peng, Weiwei Su
Summary: Naringin is a dietary flavonoid glycoside with multiple bioactivities. This study developed a rapid and sensitive method for simultaneous determination of ten flavonoid metabolites of naringin in rat urine. The results revealed the visible individual difference and low urinary recovery of flavonoid metabolites in the excretion of naringin, which may be helpful for further study to understand the in vivo behavior and action mechanism of naringin.
Article
Chemistry, Multidisciplinary
Xiao Wang, Yuze Li, Jinghan Fan, Liuying He, Junyu Chen, Huihui Liu, Zongxiu Nie
Summary: This study demonstrates the potential use of mass spectrometry and machine learning in rapidly screening genitourinary cancers using urine metabolic fingerprints. Key biomarkers were identified and dysregulated pathways were revealed, highlighting the expanding utility of urine metabolic fingerprints in disease screening.
CHEMICAL COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
G. Magliocco, F. Le Bloc'h, A. Thomas, J. Desmeules, Y. Daali
Summary: A method for quantifying melatonin and 6-hydroxymelatonin in human overnight urine using liquid chromatography-tandem mass spectrometry has been developed and validated. The study found significant correlations between urinary excretion rates of melatonin and 6-hydroxymelatonin with age, suggesting the potential of the urinary 6-hydroxymelatonin to melatonin ratio as a metric for CYP1A2 activity assessment.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Veterinary Sciences
Yong Wu, Xue-Ming Long, Gao-Feng Liu, Xia Bai, Zhi-Liang Sun, Zhao-Ying Liu
Summary: The study aimed to investigate tissue residue depletion in pigs fed G. elegans powder. Two quantitation methods, including a precise method and a semi-quantitation method, were developed and validated for the analysis of multiple components in pig tissues. The results showed that G. elegans alkaloids were widely distributed in pig tissues, and potential residue markers and target tissues were identified. The findings provide important scientific evidence for evaluating the safety of animal-derived food from G. elegans.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Ying Liang, Lin Wang, Ruipeng Zhang, Jiadi Pan, Wenhong Wu, Yuanyuan Huang, Zifan Zhang, Renbang Zhao
Summary: This study developed a universal detection method using high-performance liquid chromatography-tandem mass spectrometry to measure three typical beta-receptor agonists and investigate their metabolism in rats. The results showed that these agonists mainly existed in the form of prototype drugs in rats, and their absorption and metabolism varied between different tissues.
Article
Pharmacology & Pharmacy
Xutong Zhang, Yani Lou, Dongxu Zheng, Jialin Lu, Dansi Qi
Summary: Mu opioid receptor is currently the most important neuroaesthetics receptor in anesthesiology research, and its damage to the nervous system is still unknown. In this study, the effects of loperamide, an agonist of the mu opioid receptor, on protein expression in HT22 cells were investigated using SILAC, IMAC enrichment, and LC-MS/MS. A total of 7,823 proteins were identified. Bioinformatic analysis revealed distinct changes in the proteome of HT22 cells induced by mu opioid receptor agonism. These findings contribute to our understanding of narcotic drugs.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Chemistry, Applied
Qin Huang, Hua Zhou, Xianglun Wu, Chenli Song, Jiangang Zheng, Meikang Lei, Pengqian Mu, Pinggu Wu
Summary: This study developed a method for tracing anesthetics and sedatives in fish using liquid chromatography coupled with quadrupole linear ion trap tandem mass spectrometry. The method achieved reliable qualitative confirmation through the optimized laboratory conditions and the MRM-IDA-EPI mode. The method demonstrated good linear relationship and low detection limits, making it suitable for the determination of anesthetics and sedatives in fish.
Article
Infectious Diseases
Maria Jesus Serrano, Diego Garcia-Gonzalo, Eunate Abilleira, Janire Elorduy, Olga Mitjana, Maria Victoria Falceto, Alicia Laborda, Cristina Bonastre, Luis Mata, Santiago Condon, Rafael Pagan
Summary: This study described the excretion rate and duration of antimicrobials in pig urine during treatment, finding variations in degradation rate and total excretion time among different drugs. Despite similar effectiveness, sulfamethoxypyridazine would be the preferable choice due to only requiring waste treatment for 1 week post-treatment.
Article
Biochemical Research Methods
Fang-Fang Wu, Yan Zhu, Xu-Yang Zhao, Li-Qun Qian, Yi-Li Wei, Xiu-Juan Ma, Ming-Li Ye, Hai-Rong Cui
Summary: The study investigated the impact of molecular monomers on the construction of COFs and examined their application in dispersive solid phase extraction for alkylphenol analysis. The results showed that sheet-like COFs-II had a higher adsorption capacity and required a lower optimal amount for extraction compared to sheet-like COFs-I. The developed method achieved successful analysis of 50 textile samples, detecting alkylphenols in six samples with concentrations ranging from 1.6 to 20.9 µg/kg.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemistry & Molecular Biology
Yu Nagayoshi, Kayo Nishiguchi, Ryosuke Yamamura, Takeshi Chujo, Hiroyuki Oshiumi, Hiroko Nagata, Hitomi Kaneko, Keiichi Yamamoto, Hirotomo Nakata, Korin Sakakida, Akihiro Kunisawa, Masataka Adachi, Yutaka Kakizoe, Takanori Mizobe, Jun-ichi Kuratsu, Shinya Shimada, Yasushi Nakamori, Masao Matsuoka, Masashi Mukoyama, Fan-Yan Wei, Kazuhito Tomizawa
Summary: SARS-CoV-2 infection leads to changes in cellular RNA content, resulting in increased levels of modified nucleosides that serve as biomarkers for COVID-19 infection and severity.
Article
Chemistry, Multidisciplinary
Maria Antonio, Tania Lima, Rui Vitorino, Ana L. Daniel-da-Silva
Summary: The use of gold nanoparticles in drug delivery, photothermal or photodynamic therapy, and biosensing requires understanding of the protein corona formed on the nanoparticles' surface. In this study, the interaction between gold nanospheres, nanorods, nanoflowers, and saliva or urine was investigated. The results showed changes in the surface properties of gold nanoparticles, indicating the presence of biomolecules. Liquid chromatography with tandem mass spectrometry was used to analyze the proteins adsorbed on the nanoparticles' surface. Results indicated the adsorption of proteins with high sulfur content, and the competition between proteins and nanoparticle morphology.
Article
Medicine, Legal
Asli Atasoy, Nebile Daglioglu, Ismail Ethem Goren, Alperay Girisbay, Rukiye Aslan, Serap Annette Akgur
Summary: Synthetic cannabinoids pose a significant public health and safety issue as their ever-changing structures complicate drug tests and most cannot be detected by routine screening tests, leading to increased usage. A study found that immunoassay screening tests were insufficient and validated chromatographic methods should be used for clinical and forensic cases to accurately detect synthetic cannabinoids in urine samples.
FORENSIC SCIENCE INTERNATIONAL
(2021)
Article
Biochemical Research Methods
Sheng Yang, Xue Tian, Yu Chen, Lei Shen, Jianxin Wang
Summary: Beta 2-microglobulin (B2M) is a commonly used detection index for the early detection of kidney disease. A developed ID-LC-MS/MS method provides sensitive and reliable quantification of serum B2M, with good agreement to immunoassay. This method has been successfully applied in a clinical study involving patients with acute renal insufficiency.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Environmental Sciences
Christian Velot, Frederic Poitou, Joel Spiroux de Vendomois
Summary: There are controversies surrounding the measurement of glyphosate urinary levels using different detection methods. The study found that while ELISA method provided more reliable results for glyphosate concentrations in urine, the HPLC/Fluo technique was more accurate when applied to water samples.
ENVIRONMENTAL SCIENCES EUROPE
(2022)
Article
Microbiology
Xinxin Shan, Mengyan Yang, Nannan Wang, Stefan Schwarz, Dexi Li, Xiang-Dang Du
Summary: This study demonstrates the transfer of mobilizable poxtA-carrying plasmids in enterococci via plasmid fusion, and reveals the structural changes in the plasmids and enhanced dissemination of resistance-encoding plasmids during the fusion events.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Zijing Wang, Xiao Liu, Zhaoxin Shi, Rihong Zhao, Yalu Ji, Fang Tang, Yuan Guan, Xin Feng, Changjiang Sun, Liancheng Lei, Wenyu Han, Xiang-Dang Du, Jingmin Gu
Summary: A novel lysin, Ply1228, derived from the prophage of Streptococcus suis type 12, was found to have specific lytic activity against various S. suis strains and demonstrated therapeutic potential in a mouse model of S. suis bacteremia.
VETERINARY MICROBIOLOGY
(2022)
Article
Microbiology
Runhao Yu, Zheng Chen, Danyang Li, Stefan Schwarz, Xinwei Wang, Xiang-Dang Du
Summary: This study investigates the transfer and recombination events of a tet(A) variant conferring tigecycline resistance in Enterobacter hormaechei. The results show that the tet(A) variant was present in E. hormaechei G17 and transferred between plasmids in a two-step recombination process. This transfer of resistance genes enhances the dissemination of tigecycline resistance and compromises the effectiveness of treatment for E. hormaechei infection.
MICROBIOLOGY SPECTRUM
(2022)
Article
Veterinary Sciences
Xing-Yun Li, Rui Yu, Chunyan Xu, Yanhong Shang, Dexi Li, Xiang-Dang Du
Summary: The study aimed to determine the mobile genetic elements involved in the horizontal transfer of erythromycin resistance in Enterococcus faecalis and investigate its transmission ability in heterologous hosts. It was found that a plasmid carrying the erm(T) gene could be transferred to different bacteria and conferred resistance to erythromycin and clindamycin.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Microbiology
Xinxin Shan, Xin-Sheng Li, Stefan Schwarz, Yuxia Chen, Chunyan Xu, Xiang-Dang Du
Summary: This study demonstrates the essential role of a pheromone-responsive conjugative plasmid in the horizontal transfer of a lsa(E)-carrying genomic island in Enterococcus faecalis. The presence of this plasmid enables the dissemination of both plasmid-borne resistance genes and chromosomally-located genomic islands. This finding highlights the importance of pheromone-responsive conjugative plasmids in the genomic evolution of E. faecalis, a leading nosocomial pathogen worldwide.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Xinxin Shan, Hong Yao, Stefan Schwarz, Dexi Li, Xin-Sheng Li, Xiang-Dang Du
Summary: Traditionally, insertion sequences (ISs) and unconventional circularizable structures (UCSs) have been shown to play a major role in disseminating antimicrobial resistance genes (ARGs) in bacteria. This study identified a novel UCS on plasmid pE508-2 in E. faecalis E508, and demonstrated the excision and integration of UCS involving structures that include the erm(B) gene. The widespread presence of these UCSs in various Gram-positive bacteria highlights its importance in the dissemination of ARGs among bacterial pathogens.
VETERINARY MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Aijuan Li, Runhao Yu, Wenbo Zhao, Stefan Schwarz, Chenglong Li, Hong Yao, Xiang-Dang Du
Summary: This study reports the co-existence of tigecycline and carbapenem resistance genes in porcine Acinetobacter towneri isolate, highlighting the importance of antimicrobial resistance surveillance in food-producing animals.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Food Science & Technology
Wenbo Zhao, Wenjun Li, Xiang-Dang Du, Hong Yao
Summary: This study characterized the co-resistance and co-dissemination mechanism of carbapenem and fosfomycin resistance in an Escherichia coli strain isolated from chicken meat. The presence of the blaNDM-5 and fosA3 genes was confirmed, and their transferability to another strain was demonstrated. Whole genome sequencing revealed a novel plasmid carrying the blaNDM-5 and fosA3 genes, and the potential transfer of these genes was identified through translocation units.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Food Science & Technology
Wenjun Li, Dian Jiao, Wenbo Zhao, Chunyan Xu, Ruichao Li, Xiang-Dang Du, Hong Yao
Summary: Lincomycin is frequently used in veterinary medicine and food animal production for treating respiratory and gastrointestinal infections. This study identified a lnu(C) variant in Campylobacter from chickens that confers resistance to lincomycin. The lnu(C) variant showed amino acid substitutions compared to the originally reported lnu(C) from Streptococcus agalactiae. Cloning the lnu(C) variant into lincomycin-susceptible Campylobacter confirmed its function in increasing resistance.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Microbiology
Biao Tang, Chenhui Zou, Stefan Schwarz, Chunyan Xu, Wenbo Hao, Xiao-Mei Yan, Yuting Huang, Juan Ni, Hua Yang, Xiang-Dang Du, Xinxin Shan
Summary: This study aimed to investigate the transferability of acquired linezolid resistance genes and associated mobile genetic elements in Enterococcus faecalis isolate QZ076. By analyzing the genome and conducting conjugation experiments, it was found that the resistance genes were present on multiple plasmids and a pseudocompound transposon. Furthermore, the transfer of these resistance genes was observed between different bacterial species. This study highlights the potential for the rapid dissemination of acquired linezolid resistance genes and emphasizes the importance of monitoring and controlling their spread.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Runhao Yu, Longyu Li, Chenhui Zou, Zheng Chen, Stefan Schwarz, Sheng Chen, Chunyan Xu, Hong Yao, Xiang-Dang Du
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Infectious Diseases
Qiaojun Wang, Kai Peng, Ziyi Liu, Yan Li, Xia Xiao, Xiang-Dang Du, Ruichao Li, Zhiqiang Wang
Summary: This study aimed to determine the molecular mechanisms of linezolid-resistant enterococci (LRE) in swine slaughterhouses in China and analyze the evolutionary dynamics of poxtA-positive E. faecium worldwide. Swine faeces were found to be a significant reservoir for LRE, and optrA and poxtA were the main contributors to linezolid resistance. Phylogenetic analysis revealed the potential global transmission of CC17 poxtA-positive E. faecium between humans and animals.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Letter
Infectious Diseases
Marzia Cinthi, Sonia Nina Coccitto, Serena Simoni, Guido Zeni, Annarita Mazzariol, Antonella Pocognoli, Xiang-Dang Du, Andrea Brenciani, Eleonora Giovanetti
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Engineering, Biomedical
Hong Yao, Ruixue Zhou, Jiaming Wang, Yongbin Wei, Shihong Li, Zhenzhong Zhang, Xiang-Dang Du, Sixuan Wu, Jinjin Shi
Summary: Clinical treatment of multidrug resistant (MDR) pathogens-induced infection is a growing challenge due to the limited selection of antibiotics. Nanozymes, as artificial enzymes, have shown potential for combating MDR pathogens. However, their limited catalytic activity and inability to target pathogens precisely hinder their clinical applications.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Microbiology
Rui Yu, Yindi Xu, Stefan Schwarz, Yanhong Shang, Xuezhen Yuan, Yue Zhang, Dexi Li, Xiang-Dang Du
Summary: This study investigated the presence and location of erm(T) in clinical Streptococcus suis isolates and explored the transmission ability and fitness cost of erm(T)-carrying mobile genetic elements. The results showed that erm(T) was detected on a plasmid and an integrative and conjugative element (ICE) in S. suis isolates, which were transmissible by transformation. The erm(T)-carrying plasmid and ICE posed a fitness cost to the host S. suis isolate.
MICROBIOLOGY SPECTRUM
(2022)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
